Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (10): 86-92.doi: 10.6040/j.issn.1671-7554.0.2017.925

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Effects of mucin1-C on the biological behaviors of esophageal squamous cell carcinoma cells

GONGSUN Xin, XIN Zhongwei, SHI Mo, QIN Qiming, JIANG Bin, SONG Xuemin, LIU Xiangyan   

  1. Department of Thoracic Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2017-09-26 Online:2018-10-10 Published:2022-09-27

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Abstract: Objective To explore the expression of mucin1-C(MUC1-C)in esophageal squamous cell carcinoma(ESCC)and its role in the malignant biological behaviors of ESCC cells. Methods The expressions of MUC1-C in the ESCC tissues and normal tissues were detected with Western blotting. After MUC1-C entrance into the nucleus was inhibited with Go-201, the activity, growth and proliferation of ESCC cells were assessed with colony-forming assay and CCK-8 test; the cell migration and invasion abilities were measured with wound healing and Transwell assay, respectively; the apoptosis rate was determined with flow cytometry. Results The expression of MUC1-C in ESCC tissues was higher than that in normal tissues. The activity, growth, proliferation, migration and invasion of ESCC cells decreased while the apoptosis rate increased after MUC1-C entrance into the nucleus was inhibited with Go-201. Conclusion MUC1-C can promote the growth, proliferation, migration and invasion while inhibit the apoptosis of ESCC cells.

Key words: Esophageal squamous cell carcinoma, Mucin1-c, Migration, Apoptosis

CLC Number: 

  • R574
[1] Ferlay J, Shin HR, Bray F, et al. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008[J]. Int J Cancer, 2010, 127(12):2893-2917.
[2] Kufe DW. Mucins in cancer: function, prognosis and therapy[J]. Nat Rev Cancer, 2009, 9(12):874-885.
[3] Alam M, Ahmad R, Rajabi H, et al. MUC1-C oncoprotein activates ERK→C/EBPβ signaling and induction of aldehyde dehydrogenase 1A1 in breast cancer cells[J]. J Biol Chem, 2013, 288(43):30892-30903.
[4] Martin JC, Herbert BS, Hocevar BA. Disabled-2 downregulation promotes epithelial-to-mesenchymal transition[J]. Bit J Cancer, 2010, 103(11):1716-1723.
[5] Leng Y, Cao C, Ren J, et al. Nuclear import of the MUC1-C oncoprotein is mediated by nucleoporin Nup62[J]. J Biol Chem, 2007, 282(27):19321-19330.
[6] Takahashi H, Jin C, Rajabi H, et al. MUC1-C activates the TAK1 inflammatory pathway in colon cancer[J]. Oncogene, 2015, 34(40):5187-5197.
[7] Huang HL, Wu HY, Chu PC, et al. Role of integrin-linked kinase in regulating the protein stability of the MUC1-C oncoprotein in pancreatic cancer cells[J]. Oncogenesis, 2017, 6(7):e359. doi:10.1038/oncsis.2017.61.
[8] Bouillez A, Rajabi H, Pitroda S, et al. Inhibition of MUC1-C suppresses MYC expression and attenuates malignant growth in KRAS mutant lung adenocarcinomas[J]. Cancer Res, 2016, 76(6):1538-1548.
[9] Tagde A, Rajabi H, Bouillez A, et al. MUC1-C drives MYC in multiple myeloma[J]. Blood, 2016, 127(21):2587-2597.
[10] Kharbanda A, Rajabi H, Jin C, et al. MUC1-C confers EMT and KRAS independence in mutant KRAS lung cancer cells[J]. Oncotarget, 2014, 5(19):8893-8905.
[11] Rajabi H, Tagde A, Alam M, et al. DNA methylation by DNMT1 and DNMT3b methyltransferases is driven by the MUC1-C oncoprotein in human carcinoma cells[J]. Oncogene, 2016, 35(50):6439-6445.
[12] Siddique HR, Saleem M. Role of BMI1, a stem cell factor, in cancer recurrence and chemoresistance: preclinical and clinical evidences[J]. Stem Cells, 2012, 30(3):372-378.
[13] Hiraki M, Maeda T, Bouillez A, et al. MUC1-C activates BMI1 in human cancer cells[J]. Oncogene, 2017, 36(20):2791-2801.
[14] David JM, Hamilton DH, Claudia P. MUC1 upregulation promotes immune resistance in tumor cells undergoing brachyury-mediated epithelial-mesenchymal transition[J]. Oncoimmunology, 2016, 5(4):e1117738. doi:10.1080/2162402X.2015.1117738.
[15] Bouillez A, Rajabi H, Jin C, et al. MUC1-C integrates PD-L1 induction with repression of immune effectors in non-small-cell lung cancer[J]. Oncogene, 2017, 36(28):4037-4046.
[16] Raina D, Agarwal P, Lee J, et al. Characterization of the MUC1-C cytoplasmic domain as a cancertarget[J]. PLoS One, 2015, 10(8):e0135156. doi:10.1371/journal.pone.0135156.eCollection 2015.
[17] Sun ZG, Wang Z, Liu XY. Mucin 1 and vascular endothelial growth factor C expression correlates with lymph node metastatic recurrence in patients with N0 esophageal cancer after Ivor-Lewis esophagectomy[J]. World J Surg, 2011, 35(1):70-77.
[18] Li SH, Wang Z, Liu XY, et al. Lymph node micrometastasis: a predictor of early tumor relapse after complete resection of histologically node-negative esophageal cancer[J]. Surg Today, 2007, 37(12):1047-1052.
[19] 王衍泽, 管向臣, 史墨, 等. RNA干扰沉默黏蛋白1基因表达对食管癌细胞株Eca109顺铂耐药性的影响[J]. 中华实验外科杂志, 2017, 34(9):1476-1478. WANG Yanze, GUANXiangchen, SHI Mo, et al. Effects of silencing mucin 1 with RNA interference on drug resistance of esophageal squamous cell carcinoma Eca109 to cisplatin[J]. Chin J Exp Surg, 2017, 34(9):1476-1478.
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