Abstract: To detect the expression of LATS1 and LATS2 mRNA in human primary glioma by SYBR Green quantitative realtime PCR, and to analyse the relationship between the expression and the clinical data. Methods: The method of SYBR Green quantitative realtime PCR was established. The expression of tumor suppressor gene LATS1 and LATS2 in 30 primary glioma tissues (WHO：GradeⅡ10 cases, Grade Ⅲ10 cases and Grade Ⅳ 10 cases) and 10 normal brain tissues were quantitated by the method of standard curve. The expression differences of LATS1 and LATS2 in different grade glioma compared with the normal brain tissues were studied and the relationship of the expression and the clinical data was also analyzed by a statistical method. Results: The expressions of LATS1 and LATS2were all significantly downregulated in different grade glioma compared with normal brain tissues (P＜0.05). But there were no significant differences in the expression of LATS1 and LATS2 among the different grade glioma (P＞0.05). And there were also no significant differences in different gender and age. Conclusions: The method to detect the expression of LATS1 and LATS2 by SYBR Green quantitative realtime PCR is established successfully. The tumor suppressor genes LATS1 and LATS2 are all downregulated in human glioma compared with those in the normal brain tissues.

Key words: Large tumor suppressor, homolog 1, Large tumor suppressor, homolog 2, Glioma, Realtime polymerase chainreaction, SYBR Green