Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (5): 74-80.doi: 10.6040/j.issn.1671-7554.0.2021.1475

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Expression of aryl hydrocarbon receptor nuclear translocator-like 2 in endometrial cancer

SHEN Xiaochang1, SUN Yiqing1, YAN Lei2, ZHAO Xingbo1   

  1. 1. Department of Gynecology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250021, Shandong, China;
    2. Department of Gynecology, Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan 250001, Shandong, China
  • Published:2022-06-01

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Abstract: Objective To explore the expression and function of aryl hydrocarbon receptor nuclear translocator-like 2(ARNTL2)in endometrial cancer(EC)and its possible signal pathway. Methods The expression of ARNTL2 in EC and normal tissues was detected with immunohistochemistry, RT-PCR and Western blotting. After ARNTL2 knock down with Si-RNA(Si-ARNTL2), cell proliferation, invasion and migration were detected with CCK-8 assay, scratch healing test and Transwell assay, respectively. The epithelial-mesenchymal transition(EMT)indicators, including E-cad, N-cad, Vimentin, Snail and Slug, and Wnt/β-catenin signal pathway related proteins, including Wnt and β-catenin, were detected with Western blotting. Results Immunohistochemistry, RT-PCR and Western blotting showed that the expression of ARNTL2 was up-regulated in EC tissue(tImmunohistochemistry=2.213, P=0.033; tRT-PCR=4.766, P<0.001; tWestern blotting=2.659, P=0.012). Si-ARNTL2 transfection successfully reduced ARNTL2 expression in EC cell lines(KLE and Ishikawa). After knockdown of ARNTL2, CCK8 assay showed reduction in the absorbance of both cell lines(P<0.05); Scratch healing test showed a suppression on wound healing rate(P<0.001); Transwell invasion and migration assay showed that the number of transmembrane cells decreased(P<0.01). Western blotting showed that the expression of E-cad in the two cell lines increased, while the expressions of N-cad, Vimentin, Snail and Slug decreased(P<0.05), and the expressions of Wnt and β-catenin decreased(P<0.05). Conclusion The expression of ARNTL2 is higher in EC tissue than in normal tissue. Knockdown of ARNTL2 can inhibit cell proliferation and metastasis in EC. ARNTL2 may promote tumor proliferation and metastasis through Wnt/β-catenin pathway.

Key words: Aryl hydrocarbon receptor nuclear translocator-like 2, Endometrial cancer, Proliferation, Metastasis, Epithelial-mesenchymal transition, Signal pathway

CLC Number: 

  • R737.33
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