Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (5): 73-81.doi: 10.6040/j.issn.1671-7554.0.2020.1534

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Knockdowa of LncRNA H19 inhibits sympathetic nerve remodeling after myocardial infarction by regulating nerve growth factor

ZHANG Xueli, ZHENG Lu, WANG Yu, WANG Kang, YAN Suhua   

  1. Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan 250014, Shandong, China
  • Online:2021-05-20 Published:2021-06-01

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Abstract: Objective To explore the roles of H19 and nerve growth factor(NGF)in the development of ventricular arrhythmias(VAs)after myocardial infarction(MI). Methods MI rat models were established by left coronary artery occlusion. (1)A total of 14 healthy Sprague Dawley(SD)rats were randomly divided into the MI group and sham group, with 7 rats in each group. Seven days after MI was induced, the rats were sacrificed to determine the H19 expression with qRT-PCR. (2)A total of 32 rats were randomly divided into 4 groups: sham+NC group, sham+siH19 group, MI+NC group and MI+siH19 group, with 8 rats in each group. H19-siRNA or the control virus was injected into the left ventricular myocardium of the rats. Programmed electrical stimulation was performed 7 days after MI was induced to observe the susceptibility to VAs. The effects of H19 on NGF, tyrosine hydroxylase(TH)and growth-associated protein 43(GAP43)were detected with qRT-PCR, Western blotting and immunofluorescence staining. (3)A total of 24 rats were randomly divided into the MI+NC group, MI+siH19 group and MI+siH19+LV-NGF group for rescue test, with 8 rats in each group. The expressions of NGF, TH and GAP43 were detected with Western blotting and immunofluorescence staining. Results The expression of H19 were up-regulated 7 days after MI was induced(P<0.001). H19 knockdown suppressed the positive staining area of TH and GAP43(P<0.001), decreased the susceptibility to VAs(P<0.01), and inhibited the expression of NGF(Z=-2.402, P=0.016). The inhibitory effect of H19 knockdown on sympathetic remodeling could be saved by overexpressed NGF(PTH<0.001, PGAP43=0.001). Conclusion H19 knockdown after myocardial infarction can inhibit sympathetic nerve remodeling by regulating the expressiou of NGF, thus reducing the incidence of arrhythmia.

Key words: Myocardial infarction, Sympathetic remodeling, Ventricular arrhythmias, LncRNA H19, Nerve growth factor

CLC Number: 

  • R541
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