JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (3): 24-29.doi: 10.6040/j.issn.1671-7554.0.2015.359

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CXXC4 promotes sensitivity to chemotherapy drugs in epithelial ovarian cancer cells

ZHAO Yunxia, SONG Jing, ZHANG Cancan, LIN Xueyan, WEI Wei, TIAN Yongjie   

  1. Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2015-04-08 Online:2016-03-10 Published:2016-03-10

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Abstract: Objective To explore the effect of CXXC4 on the proliferation and sensitivity to chemotherapy drugs in ovarian carcinoma cells. Methods Protein and mRNA levels of CXXC4 were detected in nomal ovarian epithelial cells and ovarian carcinoma cells by Western blotting and Real-time PCR, respectively. The recombinant plasmid pcDNA3-CXXC4 and siRNA-CXXC4 were transfected into SKOV3, Caov3 cells and IOSE80 cells via Lipo2000. CCK8 assay was used to evaluate the effect of CXXC4 on the proliferation and sensitivity to chemotherapy drugs. Results Compared with the normal ovarian epithelial cells, CXXC4 expression was lower in ovarian carcinoma cells. Overexpression of CXXC4 inhibited the proliferation capacity and increased the sensitivity to chemotherapy drugs in SKOV3 and Caov3 cells. Decreased CXXC4 expression could promote the proliferation capacity and the chemo-resistance to chemotherapy drugs of normal ovarian epithelial cells. Conclusion Overexpression of CXXC4 could inhibit the proliferation capacity and increase the sensitivity to chemotherapy drugs in ovarian carcinoma cells, suggesting it would be a potential biomarker to predict the efficacy or a molecular target to enhance the efficacy to chemotherapy in ovarian carcinoma cells.

Key words: Ovarian cancer, Chemo-resistance, CXXC4, Proliferation

CLC Number: 

  • R737.31
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