JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (12): 12-15,32.doi: 10.6040/j.issn.1671-7554.0.2015.131

Previous Articles     Next Articles

An in vitro study of the anticancer effect of salidroside on human gastric carcinoma SGC-7901 cells

WEI Lilan, MO Shurong   

  1. Department of Physiology, Guangxi Medical University, Nanning 530021, Guangxi, China
  • Received:2015-02-02 Online:2015-12-10 Published:2015-12-10

PDF (PC)

565

Abstract: Objective To investigate the effect of salidroside on the proliferation and growth of in vitro cultured human gastric carcinoma SGC-7901 cells, and to explore the possible anti-tumor mechanism. Methods Cells were divided into control group, 0.50 mg/mL salidroside group, 1.00 mg/mL salidroside group and 2.00 mg/mL salidroside group. Cell proliferation was assessed by MTT array. Argyrophilic nucleolar organiser region proteins (AgNOR) staining was used to determine the expression of AgNOR in nuclear. The cell cycle and apoptosis were detected by flow cytometry test. Migration rate was measured by cell scratch assay. Results Compared with control group, the proliferation and AgNOR expression of SGC-7901 cells were inhibited significantly in salidroside groups (P<0.05) with a concentration-, time-and effect-depent manner; cell circle arrest increased at G2/M phase, but decreased at S phase (P<0.05); the rate of cell apoptosis increased, while cell migration rate was down-regulated (P<0.05). Conclusion Salidroside can effectively inhibit proliferation, arrest cell cycle, induce apoptosis and decrease migration rate of SGC-7901 cell.

Key words: Gastric carcinoma cells, Proliferation, Salidroside, Anti-tumor

CLC Number: 

  • R331
[1] 季加孚.我国胃癌防治研究三十年回顾[J].中国肿瘤临床, 2013, 40(22):1346-1351.
[2] 吴菲,林国桢,张晋昕.我国恶性肿瘤发病现状及趋势[J].中国肿瘤, 2012, 21(2):81-85.
[3] 宋汉君,吕少春,李丽疆,等.红景天苷的抗肿瘤作用[J].中国老年学杂志, 2011, 31(20):3991-3992.
[4] Noreen EE, Buckley JG, Lewis SL, et al. The effects of an acute dose of Rhodiola rosea on endurance exercise performance[J]. J Strength Cond Res, 2013, 27(3):839-847.
[5] Getova DP, Mihaylova AS. Effects of Rhodiola rosea extract on passive avoidance tests in rats[J]. Cent Eur J Med, 2013, 8(2):176-181.
[6] Udintsev SN, Shakhov VP. The role of humoral factors of regenerating liver in the development of experimental tumors and the effect of Rhodiola rosea extract on this process[J]. Neoplasma, 1991, 38(3):323-331.
[7] 许朝晖,王永才,张蕾,等.嗜银蛋白(AgNOR)对针吸脱落细胞癌肿早期诊断研究[J].中国实验诊断学, 2010, 14(12):2034-2035.
[8] 韩益玲,钟璐,陈芳源,等.急性非淋巴细胞白血病细胞核仁组织区相关嗜银蛋白的研究[J].上海交通大学学报:医学版, 2009, 29(1):73-75.HAN Yiling, ZHONG Lu, CHEN Fangyuan, et al. Study of argyrophilic nucleola organizer region proteins in acute myeloid leukemia cells[J]. Joumal of Shanghai Jiaotong University:Medical Science, 2009, 29(1):73-75.
[9] 李冰.温度和时间对核仁组成区嗜银蛋白染色结果的影响[J].齐齐哈尔医学院学报, 2010, 31(15):2433-2434.
[10] 曹蕊,吴雄志.中药对肿瘤细胞周期影响的研究进展[J].中国肿瘤临床, 2012, 39(10):749-752.
[11] 魏向阳,涂悦,徐忠伟,等.紫杉醇对U373细胞周期阻滞及增殖抑制的机制研究[J].中国药理学通报, 2011, 27(4):543-547. WEI Xiangyang, TU Yue, XU Zhongwei, et al. The mechanism of the induction of cell cycle arrest and inhibition on U373 cells by PTX[J]. Chinese Pharmacological Bulletin, 2011, 27(4):543-547.
[12] Feng Y, Dai X, Li X, et al. EGF signaling pathway regulates colon cancer stem cell proliferation and apoptosis[J]. Cell Prolif, 2012, 45(5):413-419.
[13] Burikhanov R, Shrestha-Bhattarai T, Qiu S, et al. Novel mechanism of apoptosis resistance in cancer mediated by extracellular PAR-4[J]. Cancer Res, 2013, 73(2):1011-1019.
[14] 何莉莎,李枋霏,孙桂芝,等.中药诱导肿瘤细胞凋亡的实验研究进展[J].河北中医, 2014, 36(2):309-311.
[15] 张晔,赵明芳,曲秀娟,等.β-榄香烯联合紫杉醇诱导胃癌细胞凋亡的体外研究[J].现代肿瘤医学, 2013, 21(5):965-968. ZHANG Ye, ZHAO Mingfang, QU Xiujuan, et al. β-elemene combined with paclitaxel induced cell apoptosis in human gastric cancer cells[J]. Modern Oncology, 2013, 21(5):965-968.
[16] 宋文瑛,许冠华,张光霁.猕猴桃根多糖对人胃癌SGC-7901细胞增殖凋亡及P-P38表达的影响[J].中国中西医结合杂志, 2014, 34(3):329-333.
[17] 马勇,吴新民,郭亚民.胃癌转移相关分子标志物的研究进展[J].现代肿瘤医学, 2014, 22(12):3017-3019. MA Yong, WU Xinmin, GUO Yamin. Research progress of molecular markers in metastasis gastric carcinoma[J]. Modern Oncology, 2014, 22(12):3017-3019.
[18] Kobayashi H, Boelte KC, Lin PC. Endothelial cell adhesion molecules and cancer progression[J]. Curr Med Chem, 2007, 14(4):377-386.
[19] 涂祥俊,邓述恺.肿瘤转移的分子机制[J].肿瘤基础与临床, 2013, 26(3):269-272.
[20] Zlotnik A, Burkhardt AM, Homey B. Homeostatic chemokine receptors and organ-specific metastasis[J]. Nat Rev Immunol, 2011, 11(9):597-606. (编辑:相峰)
[1] WANG Xiao-Lei, ZHANG Hai-Thao, ZHANG Hui, GUO Cheng-Hao. Protective effects of shuxuening injecta on vascular  endothelial  cell injury in vitro due to iodine excesses [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 38-.
[2] YIN Yue, MO Zhenfei, WU Peixin, LIU Jinxia, WEI Yuanhui, REN Jiabo, LI Chunsun. Expression of GPX1 in lung cancer cell lines and its influence on the proliferation, migration, invasion abilities and apoptosis in lung adenocarcinoma cell lines [J]. Journal of Shandong University (Health Sciences), 2026, 64(1): 65-73.
[3] HAN Jueming, WANG Hui, WU Qian, ZHENG Huiling, ZHU Lin. B4GALNT4 promotes proliferation, migration and invasion of lung adenocarcinoma cells [J]. Journal of Shandong University (Health Sciences), 2025, 63(7): 23-31.
[4] LI Guanqiang, SHI Yucheng, ZHU Kehan, HU Bo, HUANG Xianchen, SUN Yuan, LI Duxin, ZHANG Xicheng. Active peptide SK-14 derived from snail mucus promotes fibroblast proliferation and migration [J]. Journal of Shandong University (Health Sciences), 2025, 63(11): 1-7.
[5] ZHANG Jie, ZHANG Fangfang, WANG Jingnan, LI Zeyu, SONG Ying, LI Na. Expression of circ_0000144 in breast cancer and its effect on the proliferation, migration and invasion ability of breast cancer cells [J]. Journal of Shandong University (Health Sciences), 2025, 63(1): 35-42.
[6] CAO Hualin, JIA Yanzhao, QU Li, YIN Xin. Impacts of CircFAT1 on the proliferation, apoptosis and radiosensitivity of nasopharyngeal carcinoma cells by regulating miR-296-3p/MAPRE1 axis [J]. Journal of Shandong University (Health Sciences), 2023, 61(9): 38-46.
[7] JIN Shan, GAO Jie, XIE Yujiao, ZHAN Yao, DU Tiantian, WANG Chuanxin. Effects of methyltransferase PRMT5 on stabilizing USP15 to promote the occurrence and development of breast cancer [J]. Journal of Shandong University (Health Sciences), 2023, 61(7): 1-11.
[8] HE Jing, YAN Rugen, WU Zhihong, LI Changzhong. Effects of Xiaozheng Yiai decoction on the proliferation and migration of ovarian cancer SKOV3 cells [J]. Journal of Shandong University (Health Sciences), 2023, 61(5): 1-10.
[9] DONG Xiangjun, LI Juan, KONG Xue, LI Peilong, ZHAO Wenjing, LIANG Yiran, WANG Lili, DU Lutao, WANG Chuanxin. Effects of circular RNA hsa_circ_0008591 on tumor biological behavior of breast cancer cells [J]. Journal of Shandong University (Health Sciences), 2023, 61(2): 78-87.
[10] ZHAO Ge, ZOU Cunhua, SONG Dongdong, ZHAO Shuping. Effects of tanshinone IIA on the proliferation and apoptosis of endometrial carcinoma cells [J]. Journal of Shandong University (Health Sciences), 2022, 60(9): 53-58.
[11] ZHANG Zhenwei, LI Jia, CHEN Keming. IGF2BP2/m6A/ITGA5 signal axis regulates the proliferation and migration of renal clear cells [J]. Journal of Shandong University (Health Sciences), 2022, 60(9): 74-84.
[12] SHEN Xiaochang, SUN Yiqing, YAN Lei, ZHAO Xingbo. Expression of aryl hydrocarbon receptor nuclear translocator-like 2 in endometrial cancer [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 74-80.
[13] SONG Tian, FU Linlin, WANG Qiumin, YANG Xiao, WANG Ying, BIAN Yuehong, SHI Yuhua. Expression of fatty acid transport protein 1 in granulosa cells of patients with polycystic ovary syndrome [J]. Journal of Shandong University (Health Sciences), 2022, 60(2): 22-26.
[14] LU You, QIE Di, WU Jinhui, YANG Fan. Effects of the intervention of Sonic Hedgehog signaling pathway on learning and memory ability in rats with intrauterine growth restriction [J]. Journal of Shandong University (Health Sciences), 2021, 59(5): 82-89.
[15] WANG Zhengyang, XIA Yan, SHI Kaixuan, TAO Kun, WANG Xiaojie. Effects of Trametinib on PAX8 expression in ovarian cancer [J]. Journal of Shandong University (Health Sciences), 2021, 59(10): 25-31.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd