JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (11): 1-5.doi: 10.6040/j.issn.1671-7554.0.2015.185

• Preclinical Medicine •     Next Articles

Fat-derived adipokine disorder in high cholesterol diet rats

JIANG Fangjie1,2, SHAO Shanshan1,2, JING Fei1,2, YU Chunxiao2, ZHAO Jiajun1,2   

  1. 1. Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. Shandong Clinical Medical Center of Endocrinology and Metabolism, Institute of Endocrinology and Metabolism, Shandong Academy of Clinical Medicine, Jinan 250021, Shandong, China
  • Received:2015-02-13 Online:2015-11-10 Published:2015-11-10

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Abstract: Objective To investigate the effects of high cholesterol diet on adipokines (leptin, adiponectin, resistin, visfatin and glypican4) levels in serum and visceral adipose tissue (VAT). Methods A total of 60 SD rats were randomly divided into normal diet group (NC, standard diet, n=30) and high cholesterol diet group (HC, high cholesterol diet, n=30). Blood samples and VAT were collected at week 0, 4, 12 and 18, respectively. The levels of fasting blood glucose and fasting serum insulin were measured, and insulin resistance values were calculated. Adipokines in serum and VAT were detected with enzyme-linked immunosorbent assays. Results At week 18, the serum visfatin and adiponectin in the HC group were significantly increased compared with those in the NC group (P<0.05); levels of leptin, visfatin, adiponectin, and glypican4 in VAT were significantly higher in the HC group than in the NC group (P<0.05); insulin resistance was also observed in HC group. Conclusion Long-term excessive cholesterol intake induces disorder of adipokine synthesis and secretion, and such disorder may be correlated to insulin resistance.

Key words: High cholesterol diet, Adipokines, Insulin resistance

CLC Number: 

  • R589.2
[1] Goossens GH. The role of adipose tissue dysfunction in the pathogenesis of obesity-related insulin resistance[J]. Physiol Behav, 2008, 94(2):206-218.
[2] Antuna-puente B, Feve B, Fellahi S, et al. Adipokines:the missing link between insulin resistance and obesity[J]. Diabetes Metab, 2008, 34(1):2-11.
[3] Ronti T, Lupattelli G, Mannarino E. The endocrine function of adipose tissue:an update[J]. Clin Endocrinol (Oxf), 2006, 64(4):355-365.
[4] Schreibman PH, Dell RB. Human adipocyte cholesterol. Concentration, localization, synthesis, and turnover[J]. J Clin Invest, 1975, 55(5):986-993.
[5] Bluher M. Adipose tissue dysfunction in obesity[J]. Exp Clin Endocrinol Diabetes, 2009, 117(6):241-250.
[6] Guerre-Millo M, Guesnet P, Guichard C, et al. Alteration in membrane lipid order and composition in metabolically hyperactive fatty rat adipocytes[J]. Lipids, 1994, 29(3):205-209.
[7] Chaldakov GN, Stankulov IS, Hristova M, et al. Adipobiology of disease:adipokines and adipokine-targeted pharmacology[J]. Curr Pharm Des, 2003, 9(12):1023-1031.
[8] Zheng H, Zhang C, Yang W, et al. Fat and cholesterol diet induced lipid metabolic disorders and insulin resistance in rabbit[J]. Exp Clin Endocrinol Diabetes, 2009, 117(8):400-405.
[9] Bhat NR, Thirumangalakudi L. Increased tau phosphorylation and impaired brain insulin/IGF signaling in mice fed a high fat/high cholesterol diet[J]. J Alzheimers Dis, 2013, 36(4):781-789.
[10] Halaas JL, Gajiwala KS, Maffei M, et al. Weight-reducing effects of the plasma protein encoded by the obese gene[J]. Science, 1995, 269(5223):543-546.
[11] Barnes KM, Miner JL. Role of resistin in insulin sensitivity in rodents and humans[J]. Curr Protein Pept Sci, 2009, 10(1):96-107.
[12] Steppan CM, Bailey ST, Bhat S, et al. The hormone resistin links obesity to diabetes[J]. Nature, 2001, 409(6818):307-312.
[13] Fukuhara A, Matsuda M, Nishizawa M, et al. Visfatin:a protein secreted by visceral fat that mimics the effects of insulin[J]. Science, 2005, 307(5708):426-430.
[14] 孙勤, 李伶, 杨刚毅, 等. 内脂素基因过表达对胰岛素抵抗大鼠胰岛素敏感性的影响[J]. 中华医学杂志, 2008, 88(6):365-368. SUN Qin, LI Ling, YANG Gangyi, et al. Effects of visfatin gene overexpression on insulin sensitivity in the insulin-resistant rats[J]. National Medical Journal of China, 2008, 88(6):365-368.
[15] Hu E, Liang P, Spiegelman BM. AdipoQ is a novel adipose-specific gene dysregulated in obesity[J]. J Biol Chem, 1996, 271(18):10697-10703.
[16] Yamauchi T, Kamon J, Waki H, et al. The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity[J]. Nat Med, 2001, 7(8):941-946.
[17] Kubota N, Terauchi Y, Yamauchi T, et al. Disruption of adiponectin causes insulin resistance and neointimal formation[J]. J Biol Chem, 2002, 277(29):25863-25866.
[18] Gesta S, Bluher M, Yamamoto Y, et al. Evidence for a role of development genes in the origin of obesity and body fat distribution[J]. Proc Natl Acad Sci U S A, 2006, 103(17):6676-6681.
[19] Ussar S, Bezy O, Bluher M, et al. Glypican-4 enhances insulin signaling via interaction with the insulin receptor and serves as a novel adipokine[J]. Diabetes, 2012, 61(9):2289-2298.
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