Alport综合征iPSCs差异性表达新小核糖核苷酸的分析

doi:10.6040/j.issn.1671-7554.0.2015.307

Abstract

Abstract: Objective To investigate the expression profile of iPSCs of novel microRNAs between patients with Alport syndrome (AS) and normal controls (NC), and to analyze the target genes. Methods The expression profile of novel microRNAs was acquired from previously induced iPSCs, using high-throughput sequencing platform. After that, TargetScan software was adopted to predict target genes, which were then compared with reference genes, and the significantly encriched GO items and KEGG pathways were selected. Results A total of 49 differently expressed novel microRNAs were selected, 33 of which were up-regulated and 16 were down-regulated. GO enrichment analysis indicated that the target genes mainly enriched in biological regulation and metabolism; cell component and signal transduction; enzymic catalytic reaction; transporter activity. KEGG pathway analysis showed that the target genes mainly participated in metabolic pathways, purine metabolism, and cancer transcriptional regulation. Conclusion Novel microRNAs of iPSCs from AS and from NC are differently expressed, and the target genes mainly take part in molecular function, cellular component, and biological activities. Those differently expressed novel microRNAs and target genes may play an important role in the pathogenesis of AS.

Key words: Induced pluripotent stem cells, Gene ontology enrichment, microRNA, Alport syndrome, Kyoto encyclopedia of genes and genomes pathway

CLC Number:

R394-33

CHEN Wenbiao, YU Xiangqi, DAI Yong. Different expressions of novel microRNAs of iPSCs from Alport syndrome[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(9): 80-85.

References

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Different expressions of novel microRNAs of iPSCs from Alport syndrome

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