JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (9): 53-57.doi: 10.6040/j.issn.1671-7554.0.2015.296

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Over expression of lipin1 attenuates diabetic peripheral neuropathy in rats

XU Yi1, ZHUANG Xianghua1, LIU Yuantao1, SUN Aili1, LI Xiaobo1, JIANG Dongqing1, ZHANG Liang1, XU Min1, YAN Heng2, CHEN Shihong1   

  1. 1. Department of Endocrinology, Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. Department of Anesthesiology, Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2015-03-15 Online:2015-09-10 Published:2015-09-10
  • Contact: 陈诗鸿。E-mail:chenshihong26@163.com E-mail:chenshihong26@163.com

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Abstract: Objective To investigate the effects of lipin1 on diabetic peripheral neuropathy in rats. Methods Type 2 diabetes mellitus was induced in male Wistar rats which were fed with high fatty and high sugar diet for 8 weeks, followed by streptozotocin (STZ) injection intraperitoneally. And then the models were randomly divided into Lipin1 high expression (LV-Lipin1) group and empty vector (LV-control) group. Another 12 age-matched non-diabetic rats served as controls. Rats in LV-Lipin1 group were transfected with lentiviral vector expressing rat lipin1; rats in LV-control group were transfected with lentiviral empty vector. After two months, fasting blood glucose, body weight and the motor nerve conduction velocity (MNCV) were measured. Histopathological changes of sciatic nerve were analyzed with light microscope and electron microscope; Lipin1 expression was evaluated with immunohistochemistry. Results Two months after diabetes was induced, expression of Lipin1 in sciatic nerves was significantly decreased in the LV-control group compared with control group. The nerve fiber diameter, nerve fiber area and nerve fiber density in LV-control group were also decreased compared with control group. All of the above changes were significantly attenuated in LV-Lipin1 group. Motor nerve conduction velocity (MNCV) of sciatic nerve in LV-Lipin-1 group was significantly improved compared with LV-control group (P<0.05). Conclusion Expression of Lipin1 in sciatic nerve decreases in diabetic peripheral neuropathy rats. Upregulation of Lipin1 expression significantly improves the MNCV and nerve pathological morphology.

Key words: Lipin1, Peripheral neuropathy, Diabetes, Lentivirus

CLC Number: 

  • R587.1
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