JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (11): 101-105.doi: 10.6040/j.issn.1671-7554.1.2014.011

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Capillary culture method using modified semisolid Rappaport-Vassiladis medium for Salmonella detection in poultry

LIU Hui, HU Guangchun, SHI Yuwen, LI Jian, LIU Lanzheng   

  1. Department of Bacterial Disease Inspection, Jinan Municipal Center for Disease Control and Prevention, Jinan 250021, Shandong, China
  • Received:2014-04-27 Published:2014-11-10

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Abstract: Objective To develop capillary culture method using modified semisolid Rappaport-Vassiladis (MSRV) medium (referred to as modified culture method) which was introduced for preliminary screening of Salmonella in poultry products. Methods Capillary tube loading MSRV medium was inserted into liquid medium (enrichment medium or preenrichment medium) to screen Salmonella in samples. The sensitivity and specificity of the modified culture method were evaluated in 129 Salmonella strains and 58 non-Salmonella strains. The detectability of the modified culture method was assessed using poultry samples, referred to ISO 6579:2002 microbiology of food and animal feeding stuffs—horizontal method for the detection of Salmonella spp. Results The sensitivity of the modified culture method was 96.9% (125/129) for Salmonella strains, and the specificity was 96.6% (56/58) for non-Salmonella strains. The modified culture method which detected 33.3% (20/60) of poultry samples was not statistically different from ISO 6579:2002 that detected 35.0% (21/60) of poultry samples (P >0.05). Compared with ISO 6579:2002, 91.7% (33/36), negative samples did not need further isolation and identification after using the modified culture method; samples were early screened in 36 h that was half of the original time. Conclusion The detectability of the modified culture method for poultry products is not significantly different from ISO 6579:2002. The advantage of the modified culture method is saving labor, resources and time.

Key words: Motility, Capillary, Culture, Poultry, Salmonella

CLC Number: 

  • R155
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