Abstract:

Objective   To investigate the expression of tumor necrosis factor-α-induced protein 8 (TNFAIP8) in human gastric cancer tissues and its relationship with clinical and pathological features. To study the influence of TNFAIP8 on migration and invasion ability of SGC7901 in vitro. Methods   TNFAIP8 protein expressions in 30 cases of gastric cancer and cancer adjacent tissues were detected and its correlation with clinical pathological features was analyzed by the immunohistochemical staining SABC method. The liposome mediated reorganization plasmid pcDNA3.0-TIPE was transferred into SGC7901 which were cultured in vitro. The differences in the ability of gastric cancer cell invasion and migration were studied through the Transwell assay after the transfection. Results   Immunohistochemical staining results showed the expression of TNFAIP8 was significantly higher in gastric cancer compared with the adjacent tissue(P<0.001). The expression of TNFAIP8 was associated with the tumor invasion depth and lymph node metastasis(P<0.05), but not related to gender, age and tumor size(P>0.05).  Transwell assay showed the migration and invasion ability significantly enhanced when compared with the control group, and the difference was statistically significant(P<0.001). Conclusion   TNFAIP8 may play an important role in gastric cancer occurrence, development and metastasis, which can provide a new target for the treatment of gastric cancer.

Key words: TNFAIP8; Gastric cancer; Metastasis; Clinical pathology