JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (9): 39-43.doi: 10.6040/j.issn.1671-7554.0.2014.170

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COX-2 specific inhibitor induced apoptosis and autophagy in Hep-2 cells in vitro

ZHAO Yongqiang1,2, XIE Xiaoye2, CHEN Xuemei1, FENG Huiwei1, JIA Tao1, ZHANG Hui1, FAN Xianliang1   

  1. 1. Department of Otorhinolaryngology, the Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-03-26 Revised:2014-07-01 Online:2014-09-10 Published:2014-09-10
  • Contact: 范献良。E-mail:sdentfan@126.com E-mail:sdentfan@126.com

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Abstract: Objective To investigate the ability of celecoxib inducing apoptosis in Hep-2 cells and its possible mechanisms, as well as to observe the autophagy of the cells. Methods MTT was used to observe the proliferation of Hep-2 cells treated with celecoxib at different doses(0-100 μmol/L) and for different hours(0-72 hours). Cell ultrastructure was observed by electron microscope. Hep-2 cells were treated with celecoxib at different doses and for different hours and then the cell apoptosis rate was measured by flow cytometry. AIF expression was examined by Western blotting. Results Celecoxib induced a time- and dose-dependent growth inhibition in Hep-2 cells. It also induced the apoptosis of Hep-2 cells in a dose-dependent manner. No significant difference existed in the apoptosis rate of the cells treated by celecoxib for 72 and 48 hours(P>0.05). Autophagy was observed in Hep-2 cells treated by celscoxib for 72 hours. Celecoxib showed the ability of transporting AIF from mitochondria to cell nucleus. Conclusion Celecoxib can induce cell apoptosis, in which AIF mechanism may be involved. Autophagy induced by celecoxib may protect Hep-2 cells against apoptosis.

Key words: Hep-2 cells, Apoptosis, Celecoxib, Autophagy, Cyclooxygenase-2

CLC Number: 

  • R739.65
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