Abstract: To study the effects of tetromethylpragine and aminoguanidine for the metabolism of glucose and the expressions of inducible nitric oxide synthase mRNA(iNOS mRNA) in experimental insulin resistance rats. MethodsFifty male Wistar rats were randomly divided into the NC, FC, AG, TMP, and AT groups. The FC, AG, TMP and AT groups were fed with 12％ fructose, and the NC group was fed with tap water. After 3 months, the AG group was treated with 50mg／(kg·d) aminoguanidine, the TMP group with 40mg／(kg·d) tetromethylpragine, and the AT group with 40mg／(kg·d) tetromethylpragine and 50mg／(kg·d) aminoguanidine for 6 months. The levels of blood sugar, insulin, NO-2 and the expression of iNOS mRNA were determined before the experiment and at the end of the 1st, 2nd, 3rd, 5th, 7th and 9th month. ResultsThe level of blood NO-2(P＜0.01) and the expression of the iNOS mRNA (P＜0.01) were increased in the fructose control group compared with the control group and they remained at a high level till the end of 2 months′ fructose intake. The plasma insulin and the blood glucose were both increased, and the insulin sensitivity index was significantly decreased in the fructose control group compared with the control group(P＜0.01) at the end of 3 months′ fructose intake. The blood glucose was decreased(P＜0.05), the insulin sensitivity index was increased (P＜0.05), and the NO-2 and the iNOS mRNA were decreased(P＜0.01) at the end of 4 months′ treatment with tetromethylpragine and aminoguanidine. ConclusionTetromethylpragine and aminoguanidine can inhibit the expression of the iNOS mRNA and improve insulin resistance in experimental rats of fructoseinduced insulin resistance.

Key words: Fructose, Insulin resistance, Nitric oxide, iNOS mRNA, Rats, Wistar