Abstract: To investigate the effects of NEP1-40 and GM-1 in newborn rats with hypoxic ischemic brain damage (HIBD). Methods100 rats were randomly divided into 10 groups and five groups were treated in different ways at 6h: the normal control group, the shamoperation group, the HIBD model group, the GM-1 treatment group and the NEP1-40 treatment group, and the other five groups were treated at 24 h. Contents of Nogo-A mRNA in each group were determined by the method of in situ hybridization. ResultsThe expression of Nogo-A mRNA in the HIBD groups was higher than that in the control groups， and was lower in the NEP140 groups than in the HIBD groups at both time points. There were no significant differences between the GM1 group and the HIBD group when they were treated at 6?h. However, the expression of Nogo-A mRNA was lower in the GM1 group than in the HIBD group but was higher than the normal control group. Conclusion Nogo-A mRNA is significantly increased in newborn rats with HIBD. Nogo-A encoded by mRNA can inhibit the regeneration of the central nerve after injury, and NEP1-40 could antagonize the function and promotes the regeneration. GM-1 is also able to antagonize the expression of mRNA and stabilizes the cellular membrane, eases cellular edema of the injured nerve and accelerates the regeneration of new nerves.

Key words: Rats, newborn, HypoxiaIschemia, brain, Myelinassociated glycoprotein, Ganglioside, Nogo receptor antagonists