Abstract: To construct expressional vector of glycoprotein G1 and G2 of Hantavirus, GM04-38 strain, express it in Vero E6 cells, and to observe cell fusions. MethodsBy PCR and the gene cloning method, Hantavirus GM0438 strain G1 and G2 genes were cloned into plasmid pCAGGS／MCS. After being confirmed by enzyme digestion and sequence analysis, the pCAGGS-G1 and pCAGGS-G2 were co-transfected into Vero E6 cells. After being treated with acidic MEM, the cells were fixed and stained by Giemsa and were observed under microscopy. IFA was also used to observe the expression efficiencies. ResultsIFA showed the G1 and G2 genes were successfully expressed, and the fluorescent signal was robust and concentrated in the perinuclear region of the transfected cells. Giemsa staining showed that cellcell fusion could be induced under the co-expression of G1 and G2 under acidic conditions, while the coexpression of the NP gene could not enhance the fusion. ConclusionThe expressional vectors of glycoprotein G1 and G2 are successfully constructed and expressed in Vero E6 cells,

Key words: Hantavirus, Glycoprotein, Expressional vector, Cell fusion