Abstract:

Taking the disk diffusion method and broth dilution method and their “breakpoint”  standard proposed by the Clinical and Laboratory Standards Institute(CLSI) as an example, problems of these classic antimicrobial susceptibility tests were analyzed based on bacterial growth kinetics and the clinical choice of antimicrobial drugs. Then recommendations were made in the ecology of pathogens, breakpoint division, methodological criteria for antimicrobial susceptibility tests, and the antimicrobial resistance monitoring net.

Key words:  Antimicrobial susceptibility testing； Antimicrobial resistance； Antimicrobial tolerance； Breakpoint