Abstract:

Objective    To detect expression of hepatocyte cell adhesion molecule(HepaCAM) in renal clear cell carcinoma (RCCC), and to study the relativity of HepaCAM expression with occurrence and development of RCCC.  Methods    Paraffin-embedded tissue sections from 73 patients with RCCC and 12 RCCC adjacent renal tissues were included in the study.  Expression of HepaCAM in all the samples was analyzed with the immunohistochemical(PV-9001) method.   Integrated optical density(IOD) of the immunohistochemical image was estimated by Image-ProPlus 6.0 software, and the correlation of IOD and clinical features of RCCC was analyzed. Statistical analysis was performed by SPSS13.0 software. Results    HepaCAM expression was down-regulated in RCCC, while it was up-regulated in RCCC adjacent tissues. IOD in the two groups was 8203.87±8431.88 and 36639.28±20851.94, respectively(P<0.05). Expression of HepaCAM had no relation with clinical features such as gender, age, the tumor size, the T stage and TNM stage. Conclusions     HepaCAM expression is lower in RCCC compared with RCCC adjacent tissues, and may has a close relation with the development of RCCC. HepaCAM is supposed to act as a marker for carcinogenesis in the kidney.

Key words: Renal clear cell carcinoma;  Hepatocyte cell adhesion molecule; Immunohistochemistry