Abstract:

Objective    To study the expressions of Th17 lymphocytes related cytokines including interleukin 17A(IL17A) and interleukin 23(p19/p40)(IL23p19/p40) in prostate tissue of experimental autoimmune prostatitis(EAP) of C57BL/6 mice. Methods  Prostate glands from Wistar rats were used to make purified prostate protein, and then the mice were subcutaneously injected with the purified protein combined with complete Freund′s adjuvant(CFA) and diphtheria pertussis tetanus (DPT) vaccine at 0 and 30 days. 30 days after the second injection the prostates of the immune mice were observed, and one-step real-time RT-PCR was used to check mRNA expressions of Th17 lymphocytes related cytokines including IL17A and IL23 (p19/p40) in the prostate tissue of EAP mice, and the differences of the cytokines between the EAP and control groups were compared to study the effectiveness of the cytokines in the onset of autoimmune prostatitis of C57BL/6 mice. Results  The mice model of EAP was successfully made. The mRNA relative amounts of IL17A, IL23p19 and IL23p40 in the mice prostate of the  EAP and the control group were 0.0530±0.0265 vs 0.0177±0.0065， 0.0289±0.0283 vs 0.0036±0.0029， 0.0051±0.0025 vs 0.0041±0.0028 respectively, and the differences between the two groups were significant  (all P＜0.05). Conclusion    lymphocytes related cytokines in the prostate tissue of EAP mice are higher than those in the controls, and these cytokines are related to the onset of EAP.

Key words: Chronic prostatitis; T-Lymphocytes; Interleukin-17; Interleukin-23