JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2009, Vol. 47 ›› Issue (12): 17-20.

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ZHANG Xiaoli 1 , LI Xiao 2 , WANG Xin 1 , ZHENG Lin 1 , ZHAO Weiming 1 , QI Mei 1   

  1. 1. Department of Medical Microbiology, School of Medicine; 2. School of Stomatology, Shandong University, Jinan 250012, China
  • Received:2009-10-15 Online:2009-12-16 Published:2009-12-16

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Abstract:

Uo study the possibility of the human papillomavirus type 58 early gene E2 as a candidate antigen of DNA vaccine. MethodsRecombinant eukaryotic vector pcDNA3/58E2 was constructed and was transfected into SiHa cells. HPV58 E2 mRNA transcripts were examined by RTPCR.  BALB/C mice were immunized by muscle injection with plasmid pcDNA3/58E2, and serum specific antiHPV58E2 IgG was detected by ELISA. ResultsHPV58 E2 mRNA transcripts were detectable in SiHa cells by RTPCR, and specific antiHPV58E2 IgG could be induced in mice immunized with plasmid pcDNA3/58E2. ConclusionThe recombinant plasmid pcDNA3/58 E2 was successfully constructed, and it could stimulate an E2 specific immune response in mice as a DNA vaccine.

 

Key words:  , Human papillomavirus type 58(HPV58), Gene, E2, DNA vaccine, Cervical cancer

CLC Number: 

  • Q344.13
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