Abstract:

To study the effects of KI on proliferative activity of human skin fibroblasts at different doses. MethodsHuman skin fibroblasts (HSF) were treated with KI 0, 1?000,3?000,5?000,7?000,9?000, and 11?000?μg/L for 24?h and to observe the morphology of fibroblasts.  The methods of FCM and ELISA were used to detect the proliferation activity of human skin fibroblasts. ResultsKI at a certain range of concentration(＜5?000?μg/L)could significantly increase proliferative activity of human skin fibroblasts but decreases the proliferative activity at a certain range of concentration(＞7?000?μg/L). The proliferative activity was the highest from 5?000?μg/L to 7?000?μg/L, but there were no significant differences between them. ConclusionKI with an exposure level＜5?000?μg/L can increase the proliferative activity of human skin fibroblasts, whereas it decrease the activity when its exposure ＞7?000?μg/L.

Key words:  Fibroblasts; Potassium iodide；Cell culture; ProliferationThorn