JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Effects of hCG on invasive activity of HTR8SVneo trophoblastic
cells and its mechanism

LI Zewu1, YANG Meixiang2, SUN Jintang2, XIE Qi2, DENG Biping2,
LIU Jia2,　SONG Bingfeng2, MAO Haiting2, QU Xun2
   

  1. (1. Institute of Basic Medical Sciences, Shandong Academy of Medical Sciences, Jinan 250062, China;
    2. Basic Medical Science Institute，Qilu Hospital of Shandong University， Jinan 250012， China)
  • Received:1900-01-01 Revised:1900-01-01 Published:2009-04-16
  • Contact: QU Xun

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Abstract: To study the effect of hCG on invasion of HTR8SVneo cell line and to explore its mechanism. MethodsFirst, cultured HTR8SVneo cells were divided into the control group, the low concentration of hCG (10?IU/mL) to stimulate group, and the high concentrations of hCG (100?IU/mL) to stimulate group, then for further experiments they were divided into the control group, the hCG (100?IU/mL) stimulation group, and the DPCPX (A1R specific blocker) +hCG (100?IU/mL) joint stimulation group. The transcription levels of MMP2 in HTR8SVneo cells were determined by RTPCR, and the secreting protein trend of MMP2 was determined by gelatin mass spectrometry. ResultsProduction of MMP2 protein was consistent with levels of MMP2 mRNA treated with different concentration hCG. Expression of A1R was increased at high concentration (100?IU/mL), however, levels of MMP2 mRNA and its secreted protein were reduced after blocking the A1R by DPCPX. ConclusionhCG promotes HTR8SVneo cell invasion by increasing expression of A1R.

Key words: Trophoblastic cell, Human chorionic gonadotropin, Adenosine receptor, Matrix Metalloproteinase

CLC Number: 

  • R714.12
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