JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

• 无栏目 • Previous Articles     Next Articles

Neuroprotective effects of insulinlike growth factor 1 on cultured dorsal
root ganglion neurons with neurotoxicity induced by glutamate

ZHANG Fengjun, WANG Lihong, LIU Zhen, XING Ziying, WANG Huaijing, LI Zhenzhong
   

  1. (Department of Anatomy, School of Medicine, Shandong University, Jinan 250012, China)
  • Received:1900-01-01 Revised:1900-01-01 Published:2009-04-16
  • Contact: LI Zhenzhong

PDF (PC)

163

Abstract: To determine the neuroprotective effects of insulinlike growth factor 1 (IGF1) on cultured dorsal root ganglion (DRG) neurons injured by glutamate (Glu). MethodsDRG neurons of Wistar rat embryos were cultured for 48?h in vitro and then exposed to Glu (200?μmol/L) with or without IGF1 (10?nmol/L). The living cells were observed with an inverted contrast microscope. The cultures were processed for detecting the apoptosis rate using flow cytometry and for detecting the fluorescent intensity of intracellular Ca2+ using confocal laser scanning microscopy (CLSM). ResultsSurvival status of the DRG cells with IGF1 incubation was better than that without IGF1 incubated cultures. The apoptosis rate and fluorescent intensity of Ca2+ were lower in cultures with IGF1 than those without. ConclusionIGF1 may reduce intracellular Ca2+ concentration, inhibits neuronal apoptosis, and protects the DRG neurons from neurotoxicity induced by Glu.

Key words: Insulinlike growth factor 1, Ganglion, Neuron, Glutamate, Rat,Wistar

CLC Number: 

  • R322.85
[1] ZHANG Dao-Lai, SUN Tao, XIE Shan-Shan, WANG Yu-Zhuo, ZHAO Ling, FENG Yu-Xin, XIN Hua. Developmental expression of NMDAR1 subunit on cultured cerebral cortical neurons of fetal rats in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 28-32.
[2] ZHANG Hongjia, YAO Wenhuan, KOU Ruirui, CHENG Dong, ZHANG Tianliang, ZHAO Xiulan. Effect of alone and combined exposure to cadmium and microplastics on cognitive function in mice [J]. Journal of Shandong University (Health Sciences), 2025, 63(3): 44-54.
[3] LIU Wenzhu, LIU Fuchen. Differentiation and molecular pathological analysis of motor neurons in a case of spinal muscular atrophy [J]. Journal of Shandong University (Health Sciences), 2024, 62(4): 92-100.
[4] LU You, QIE Di, WU Jinhui, YANG Fan. Effects of the intervention of Sonic Hedgehog signaling pathway on learning and memory ability in rats with intrauterine growth restriction [J]. Journal of Shandong University (Health Sciences), 2021, 59(5): 82-89.
[5] ZHAO Yongheng, GAO Liang, LI Baomin. Two cases of anti-Ma2 antibody positive encephalitis in children and literature review [J]. Journal of Shandong University (Health Sciences), 2021, 59(5): 96-103.
[6] ZHANG Chunyun, HE Wei, JIANG Bin, WEI Zhaosheng, WANG Zhigang. Safety and efficacy of neuronavigation-guided minimally invasive aspiration for 17 patients of supratentorial intracerebral hemorrhage with hernia [J]. Journal of Shandong University (Health Sciences), 2020, 58(2): 44-48.
[7] XING Qianqian, FU Zhijian. Dorsal root ganglion pulsed radiofrequency combined with lumbar lateral recess block in the treatment of lumbar spinal stenosis [J]. Journal of Shandong University (Health Sciences), 2018, 56(7): 76-80.
[8] ZHANG Lihong, LI Xian, WANG Linsheng, LI Honglei, LI Lei. Fat-containing ganglioneuroma: imaging features and clinicopathological correlation [J]. Journal of Shandong University (Health Sciences), 2018, 56(12): 73-78.
[9] YU Yujuan, GAO Chengjie, WANG Xiaopeng, LI Bo. Effects of parecoxibsodium on inflammatory response and its neuroprotection in patients undergoing brain tumor surgery [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(6): 65-68.
[10] LI Guohua, CHI Heng, XING Huimin, TANG Jiyou. Effects and potential mechanism of orexin-A on hippocampal neurons under hypoxia condition [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 17-24.
[11] ZANG Lijiao, QIU Jie, ZHUANG Genmiao, AN Li. Correlation among serum S100B protein, NSE and neonatal hypoglycemic brain damage [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 51-54.
[12] WANG Limin, WANG Xudong. Effects of oxygen with different concentrations on the expressions of S100β protein and neuron-specific enolase after cardiopulmonary resuscitation in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(3): 5-8.
[13] ZHANG Zhiqiang, WU Yanhua, WANG Maoshui, WANG Xinfeng, WANG Yunshan. Diagnostic value of combination of PCT and NSE in serum for detecting small cell lung cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(11): 36-39.
[14] HAO Fengqin, LI Na. Neuroprotective effect of flavanoids extracted from onion on retinal ganglion cells with diabetes mellitus [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(1): 7-10.
[15] ZHANG Kai, LIANG Fei, HAN Bo, MA Xiaochun, ZHU Xiaolong, ZHANG Jun, ZHANG Tao, ZOU Chengwei. Radiofrequency modified maze Ⅲ plus ganglionic plexus ablation vs radiofrequency modified maze Ⅲ alone in the treatment of atrial fibrillation with rheumatic mitral lesion [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(5): 66-70.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd