JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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PDGFC variant mRNA expression in renal cell carcinoma cells

LIU Juanping1, LIU Zhaoxu1，2, XU Zhonghua2, LIU Cheng2, ZHOU Zunlin2, YAN Lei2, FAN Yidong2

  

  1. （1. School of Nursing，Shandong University, Jinan 250012， China;
    2. Department of Urology, Qilu Hospital of Shandong University, Jinan 250012， China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-03-16 Published:2009-03-16

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Abstract: To explore expressions of Plateletderived growth factor C(PDGFC) and a novel spliced variant (designated as PDGFCb) of human PDGFC in clear cell renal cell carcinoma and to explore its significance. MethodsUsing semiquantitative reverse transcriptionpolymerase chain reaction (RTPCR) method, expressions of PDGFC and PDGFCb in 12 clear cell renal cell carcinoma tissues and matched adjacent normal tissues were determined. The relationship between expressions of PDGFC and PDGFCb and the impact of Cisplatin on mRNA expression of PDGFC and PDGFCb in the renal cell line was analyzed. ResultsCompared with the matched normal tissues, 9 of the 12 samples had a decreased PDGFCb mRNA expression, 10 had an increased PDGFC expression, and 11 had an increased PDGFC/Cb expression. On the mRNA level, Cisplatin downregulated expressions of PDGFC and PDGFCb and the effect on the latter was dosedependently. Expressions of PDGFC and PDGFCb between the carcinoma tissues and the matched normal tissues were significantly different and expression of PDGFC was upregulated in the carcinoma tissues. ConclusionPDGFCb presents a reverse regulation on the secretion of PDGFC, and this might be involved in RCC tumorigenesis. The downregulation of PDGFC and Cb induced by Cisplatin provides a potential pathway for research of the tumorigenesis of renal cell carcinoma.

Key words: Kindey neoplasms, Cisplatin, Plateletderived growth factorC

CLC Number: 

  • R737.11
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