关键词: 凋亡, 药物敏感性 , 反义, 增殖, survivin 寡核苷酸类, 乳腺肿瘤

Abstract: Objective To investigate the effects of surviving antisense oligonucleotide. (ASODN) on the cell proliferation，apoptosis and chemosensitivity to paclitaxel in human breast cancer cells MCF-7. Methods SurvivinASODN was transfected into MCF-7cells mediated by Lip reagent. The expression of survivin mRNA was determined by RT-PCR, the cell proliferation and chemosensitivity to paclitaxel in MCF-7 were determined by MTT assay, and the apoptosis and cell cycle were determined by flow cytometry. Results Antisense compound efficiently downregulated the survivin mRNA expression in a timedependent manner. The growth inhibition rate of MCF-7 was decreased in a dose- and time-independent manner. The cell cycle was arrested at G2/M phase; apoptosis was obviously found. Paclitaxel could enhance the expression of survivin at early time, and this effect might play a critical role in the escape of apoptosis induced by paclitaxel, which may promote the resistance of paclitaxel. The antisense compound efficiently down-regulated this induction by paclitaxel, and enhanced the chemosensitibity to paclitaxel in MCF-7. Conclusion Survivin antisense oligonucleotide effectively suppresses the expression of survivin mRNA, increases the apoptosis and enhances the chemosensitivity to paclitaxel in MCF-7.

Key words: Breast neoplasms, Survivin oligoribonucleotides, antisense, Cell proliferation, Apoptosis, Chemosensitivity