关键词: 巩膜 , B细胞淋巴瘤/白血病2基因, 细胞凋亡, 碱性成纤维细胞生长因子, 近视

Abstract: Objective：To explore the expression of B-cell lymphoma/leukemia 2 and basic fibroblast growth factor in lens-induced myopia sclera of guinea pigs and evaluate the effect of the two factors on apoptosis of the sclera cells. Methods: A -20D concave or -10D concave lens was worn over the right eye in 30 fourweek old pigmented guinea pigs. The eyes were periodically examined by retinoscopy, and A-scan ultrasonography. Two weeks later, the eyeballs were removed and the apoptotic cells were determined by electron microscopy, TUNEL technique and flow cytometry in scleras. Both bFGF and the bcl-2 protein were determined by the immunohistochemisty method and the Western-blot method. Results: All eyes treated with -10.00D or -20.00D concave lens produced defocus-induced myopia. Axial lengths in lensinduced eyes remarkably increased compared with control eyes. Apoptotic cells were observed in fibroblasts of sclera in lens-induced eyes, much more than in control eyes (P＜0.01). Expression of the bFGF and bcl-2 protein was decreased (P＜0.01). With an increase of the diopter in the lens-induced myopia of guinea pigs, expression of bFGF and bcl-2 was decreased. Conclusion: bFGF and bcl-2 may play critical roles in guinea pig′s sclera with lens-induced myopia by adjustment of the apoptosis.

Key words: Myopia, Apoptosis, Fibroblast growth factor2, B-cell lymphoma/leukemia 2, Sclera