山东汉族人群ApoE基因多态性与房颤的相关性

doi:10.6040/j.issn.1671-7554.0.2017.097

摘要/Abstract

摘要： 目的初步探讨山东汉族人群ApoE基因 112位点和158位点基因多态性与房颤发生的相关性。方法经临床诊断为房颤患者92例(房颤组),同期体检者93例(对照组),收集所有患者一般临床资料,并收集全血,提取全血DNA,采用芯片法检测其ApoE基因112位点和158位点基因多态性。计数资料之间的比较采用χ²检验,计数小于5时选用Fisher精确检验法;计量资料比较均采用t检验;房颤发生相关多因素分析采用二元Logistic回归分析。结果房颤组与对照组相比,ApoE基因112位点基因型频率、等位基因频率差异有统计学意义(P<0.05);158位点基因型频率、等位基因频率差异无统计学意义(P>0.05);载脂蛋白E表型差异有统计学意义(P<0.05),ApoE4型者房颤发生率高。结论山东汉族人群ApoE基因多态性与房颤发生具有一定的相关性,ApoE4是房颤的敏感表型,携带ApoE4型者罹患房颤的可能性更大。

关键词: 载脂蛋白E, 多态性, 房颤, 炎症, 氧化应激

Abstract: Objective To investigate the association of the polymorphism of codon 112 and codon 158 in the ApoE gene and risk of atrial fibrillation(AF)in Han people of Shandong Province. Methods A total of 92 diagnosed AF cases were included in the AF group and 93 hospitalized patients over the same period were selected as control group. The general clinical information and whole blood of all patients were collected, and then the genomic DNA was extracted. The polymorphisms of 112 locus and 158 locus in ApoE gene were detected with microarray. The count data were compared with chi square test and the Fisher exact test was used when the count was less than 5. The measurement data were compared with t test. The AF-related factors were analyzed with binary Logistic regression analysis. Results There were significant differences in the genotype frequency and allele frequency at codon 112 between the AF group and control group(P<0.05), while there were no significant difference at codon 158(P>0.05). There was statistical difference in the phenotype frequency of ApoE between the AF group and control group(P<0.05). People who carried ApoE4 had a higher risk of AF. Conclusion ApoE gene polymorphism is associated with the risk of AF among Han people in Shandong Province. ApoE4 is a sensitive phenotype and people who carry ApoE4 are more likely to develop AF than those who do not.

Key words: Apolipoprotein E, Polymorphism, Atrial fibrillation, Inflammation, Oxidative stress

中图分类号:

R714.252

张秀玲,张磊,刘丹,梁江久. 山东汉族人群ApoE基因多态性与房颤的相关性[J]. 山东大学学报 (医学版), 2018, 56(3): 79-84.

ZHANG Xiuling, ZHANG Lei, LIU Dan, LIANG Jiangjiu. Association of ApoE gene polymorphism and risk of atrial fibrillation among Han people in Shandong Province[J]. Journal of Shandong University (Health Sciences), 2018, 56(3): 79-84.

参考文献

[1] Ruiz JR, Labayen I, Ortega FB, et al. Birth weight and blood lipid levels in Spanish adolescents: influence of selected APOE, APOC3 and PPARgamma2 gene polymorphisms. The AVENA Study[J]. BMC Med Genet, 2008, 9(10): 98.
[2] Torres-Perez E, Ledesma M, Garcia-Sobreviela MP, et al. Apolipoprotein E4 association with metabolic syndrome depends on body fatness[J]. Atherosclerosis, 2016, 245: 35-42.
[3] Cheema AN, Bhatti A, Wang X, et al. APOE gene polymorphism and risk of coronary stenosis in Pakistani population[J]. Biomed Res Int, 2015: 1-5.
[4] Karahan Z, Ugurlu M, Uçaman B, et al. Relation between Apolipoprotein E gene polymorphism and severity of coronary artery disease in acute myocardial infarction[J]. Cardiol Res Pract, 2015: 1-4.
[5] Abd El-Aziz TA, Mohamed RH. LDLR, ApoB and ApoE genes polymorphisms and classical risk factors in premature coronary artery disease[J]. Gene, 2016, 590(2): 263-269.
[6] Raygani AV, Rahimi Z, Kharazi H, et al. Association between apolipoprotein E polymorphism and serum lipid and apolipoprotein levels with Alzheimer's disease[J]. Neurosci Lett, 2006, 408(1): 68-72.
[7] Raygani AV, Zahrai M, Raygani AV, et al. Association between apolipoprotein E polymorphism and Alzheimer disease in Tehran, Iran[J]. Neurosci Lett, 2005, 375(1): 1-6.
[8] Das S, Kaul S, Jyothy A, et al. Association of APOE(E2, E3 and E4)gene variants and lipid levels in ischemic stroke, its subtypes and hemorrhagic stroke in a South Indian population[J]. Neurosci Lett, 2016, 628: 136-141.
[9] Watanabe H, Tanabe N, Yagihara N, et al. Association between lipid profile and risk of atrial fibrillation[J]. Circ J, 2011, 75(12): 2767-2774.
[10] Alonso A, Yin X, Roetker NS, et al. Blood lipids and the incidence of atrial fibrillation: the multi-Ethnic study of atherosclerosis and the framingham heart study[J]. J Am Heart Assoc, 2014, 3(5): 1211-1221.
[11] Wong CX, Ganesan AN, Selvanayagam JB. Epicardial fat and atrial fibrillation: current evidence, potential mechanisms, clinical implications, and future directions[J]. Eur Heart J, 2017, 38(17): 1294-1306.
[12] Liu F, Li Y, Xu Y. Weight loss to prevent atrial fibrillation: The role of epicardial adipose tissue[J]. Int J Cardiol, 2016, 204: 124-129.
[13] 魏涛涛, 郭雪娅. 心房颤动新型危险因素的研究进展[J]. 中国循环杂志, 2016, 31(7): 720-722.
[14] Lee JJ, Yin X, Hoffmann U, et al. Relation of pericardial fat, intrathoracic fat, and abdominal visceral fat with incident atrial fibrillation(from the Framingham Heart Study)[J]. Am J Cardiol, 2016, 118(10): 1486-1492.
[15] Garcia-Sayan E, Patel M, Wassouf M, et al. Derivation and validation of E/e' ratio as a parameter in the evaluation of left atrial appendage thrombus formation in patients with nonvalvular atrial fibrillation[J]. Int J Cardiovasc Imaging, 2016, 32(9): 1349-1356.
[16] Nalliah CJ, Sanders P, Kottkamp H, et al. The role of obesity in atrial fibrillation[J]. Eur Heart J, 2016, 37(20): 1565-1572.
[17] Jofre-Monseny L, Minihane AM, Rimbach G. Impact of apoE genotype on oxidative stress, inflammation and disease risk[J]. Mol Nutr Food Res, 2008, 52(1): 131-145.
[18] Huebbe P, Lodge JK, Rimbach G. Implications of apolipoprotein E genotype on inflammation and vitamin E status[J]. Mol Nutr Food Res, 2010, 54(5): 623-630.
[19] 吴丹丹, 陈瑜, 张腾. 房颤发病机制研究新进展[J]. 中西医结合心脑血管病杂志, 2016, 4(12): 1342-1346.
[20] Hu YF, Chen YJ, Lin YJ, et al. Inflammation and the pathogenesis of atrial fibrillation[J]. Nat Rev Cardiol, 2015, 12(4): 230-243.
[21] Bas HA, Aksoy F, Icli A, et al. The association of plasma oxidative status and inflammation with the development of atrial fibrillation in patients presenting with ST elevation myocardial infarction[J]. Scand J Clin Lab Invest, 2017, 77(2): 77-82.
[22] Harada M, Van Wagoner DR, Nattel S. Role of inflammation in atrial fibrillation pathophysiology and management[J]. Circ J, 2015, 79(3): 495-502.
[23] Rollo J, Knight S, May HT, et al. Incidence of dementia in relation to genetic variants at PITX2, ZFHX3, and ApoE epsilon4 in atrial fibrillation patients[J]. Pacing Clin Electrophysiol, 2015, 38(2): 171-172.
[24] Velagaleti RS, Massaro J, Vasan RS, et al. Relations of lipid concentrations to heart failure incidence: the Framingham Heart Study[J]. Circulation, 2009, 120(23): 2345-2351.
[25] Rye KA, Barter PJ. Cardioprotective functions of HDLs[J]. J Lipid Res, 2014, 55(2): 168-179.
[26] Guo Y, Lip GYH, Apostolakis S. Inflammation in atrial fibrillation[J]. J Am Coll Cardiol, 2012, 60(22): 2263-2270.
[27] Yang KC, Dudley SC. Oxidative stress and atrial fibrillation: finding a missing piece to the puzzle[J]. Circulation, 2013, 128(16): 1724-1726.
[28] Eichner JE, Dunn ST, Perveen G, et al. 2002. Apolipoprotein E polymorphism and cardiovascular disease: a HuGE review[J]. Am J Epidemiol, 2002, 155(6): 487-495.
[29] Lee JJ, Yin X, Hoffmann U, et al. Relation of pericardial fat, intrathoracic fat, and abdominal visceral fat with incident AF(from the Framingham Heart Study)[J]. Am J Cardiol, 2016, 118(10): 1486-1492.
[30] Luscher TF. Atrial fibrillation: novel risk factors, mechanisms and ablation techniques[J]. Eur Heart J, 2016, 37(20): 1553-1555.
[31] Scridon A, Dobreanu D, Chevalier P, et al. Inflammation, a link between obesity and atrial fibrillation[J]. Inflamm Res, 2015, 64(6): 383-393.
[32] Zhu Y, Nwabuisi-Heath E, Dumanis SB, et al. APOE genotype alters glial activation and loss of synaptic markers in mice[J]. Glia, 2012, 60(4): 559-569.

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