成人脂肪细胞β3肾上腺素受体表达与肥胖的相关性

doi:10.6040/j.issn.1671-7554.0.2017.750

摘要/Abstract

摘要： 目的探讨成人脂肪细胞 β3 肾上腺素受体(β3AR)表达水平的变化及与体质量指数(BMI)的关系,为通过干预 β3AR 信号通路治疗肥胖症提供理论依据。方法纳入择期腹部手术患者42例,取脂肪组织,根据BMI分为超重组(25≤BMI<30)和正常体质量组(BMI<25)。通过实时定量PCR和Western blotting技术检测成熟脂肪细胞、前脂肪细胞中β3AR的mRNA及蛋白表达水平。结果超重组成熟脂肪细胞 β3AR mRNA(t=2.594, P=0.013)和蛋白(t=2.760, P=0.010)及前脂肪细胞β3AR蛋白(t=2.442, P=0.023)表达水平均低于正常体质量组。成熟脂肪细胞 β3AR mRNA表达与BMI呈负相关关系(r=-0.406, P=0.008)。结论肥胖前期的超重患者脂肪细胞 β3AR 表达水平下调,可能参与肥胖的发生过程。

关键词: β3肾上腺素受体, 脂肪细胞, 超重, 成人

Abstract: Objective To detect relationship between adipocyte β3AR expression and adiposity in adults, and to explore the potential therapeutic target for obesity. Methods A total of 42 adipose tissues were collected from the patients who accepted abdominal operation and divided into overweight group(25≤BMI<30)and normal weight group(BMI<25). β3AR expressions in mRNA and protein levels were detected by qRT-PCR and Western blotting. Results The β3AR mRNA of mature adipocyte(t=2.594, P=0.013), β3AR protein level of mature adipocyte(t=2.760, P=0.010)and preadipocyte(t=2.442, P=0.023)in overweight group were lower than that in normal weight group. There was negative correlation between β3AR mRNA expression and BMI(r=-0.406, P=0.008). Conclusion The down-regulation of adipocyte β3AR expression in overweight group may play an important role in development of obesity.

Key words: β3 adrenergic receptor, Adipocyte, Overweight, Adult

中图分类号:

R589.2

曹文悦,蒲迎,刘钊,郭峰,于静,尹晓. 成人脂肪细胞β3肾上腺素受体表达与肥胖的相关性[J]. 山东大学学报 (医学版), 2018, 56(1): 81-85.

CAO Wenyue, PU Ying, LIU Zhao, GUO Feng, YU Jing, YIN Xiao. Relationship between adipocyte β3 adrenergic receptor expression level and adiposity in adults[J]. Journal of Shandong University (Health Sciences), 2018, 56(1): 81-85.

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