miR-25-3p在40例子宫内膜腺癌组织中的表达及对KLE细胞生物学功能的影响

doi:10.6040/j.issn.1671-7554.0.2020.0906

摘要/Abstract

摘要： 目的探讨miR-25-3p在子宫内膜腺癌组织中的表达及其对KLE细胞增殖、迁移、侵袭、凋亡的影响。方法选取2018年1月至12月在河北医科大学第四医院妇科行手术治疗的子宫内膜腺癌患者40例,采用Real-time PCR方法检测癌和癌旁正常组织中miR-25-3p的表达水平;应用细胞转染技术将miR-25-3p模拟物转染至子宫内膜腺癌细胞KLE中,转染后分为过表达组和对照组;采用Real-time PCR方法检测转染后两组KLE细胞中miR-25-3p的表达水平;分别应用流式细胞术、Transwell试验、基质胶试验及流式细胞Annexin v-PI 双染实验检测两组细胞的增殖、迁移、侵袭及凋亡能力。结果 miR-25-3p在40例子宫内膜腺癌和癌旁组织中的相对表达量分别为0.38±0.91、0.17±0.51,癌组织表达高于癌旁组织,差异有统计学意义(t=12.28,P=0.003);增殖实验检测结果显示,两组增殖指数分别为(59.41±0.78)%、(40.69±0.78)%,过表达组高于对照组,差异有统计学意义(各期两组间均值差异均有统计学意义: G0G1 期t=12.504, P<0.001; S 期t=6.902, P=0.020; G2M期t=4.203, P=0.014;PI期t=12.475,P<0.001);迁移实验检测结果显示,两组迁移细胞个数分别为(28.54±1.73)个、(19.21±1.62)个,过表达组高于对照组,差异有统计学意义(t=8.404, P=0.001);侵袭实验检测结果显示,两组的侵袭细胞个数分别为(36.66±1.53)个、(17.66±1.53)个,过表达组高于对照组,差异有统计学意义(t=15.234, P<0.001);凋亡检测结果显示,两组的细胞凋亡率分别为(2.75±0.58)%、(4.81±0.31)%, 过表达组低于对照组,差异有统计学意义(t=5.443, P=0.006)。结论 miR-25-3p在子宫内膜腺癌组织中呈高表达。过表达miR-25-3p可能促进子宫内膜腺癌细胞KLE的增殖、迁移和侵袭,抑制细胞的凋亡。

关键词: miR-25-3p, 子宫内膜腺癌, 增殖, 迁移, 侵袭, 细胞凋亡

Abstract: Objective To investigate the expression of miR-25-3p in endometrial cancer tissues, and to explore its effects on the proliferation, migration, invasion and apoptosis of KLE cells. Methods A total of 40 patients with endometrial cancer treated during Jan. and Dec. 2018 were enrolled. The cancer tissues and adjacent tissues were collected, and the miR-25-3p expressions were detected with Real-time PCR. After KLE cells were transfected with miR-25-3p mimics, they were divided into overexpression group and control group, whose miR-25-3p expressions were detected with Real-time PCR. The proliferation, migration, invasion and apoptosis of the two groups of KLE cells were detected with flow cytometry, Transwell assay, matrigel invasion assay, and Annexin v-PI double staining assay, respectively. Results The expression of miR-25-3p was 0.38±0.91 in cancer tissues and 0.17±0.51 in the adjacent tissues, and the difference was statistically significant (t=12.28, P=0.003). Flow cytometry showed the proliferation index was(59.41±0.78)% in the overexpression group and(40.69±0.78)% in the control group, and the difference was statistically significant(G0G1 phase: t=12.504, P<0.001; S phase: t=6.902, P=0.020; G2M phase: t=4.203, P=0.014; PI phase: t=12.475, P<0.001). Transwell assay showed the number of migration cells was 28.54±1.73 in the overexpression group and 19.21±1.62 in the control group, and the difference was statistically significant(t=8.404, P=0.001). Matrigel invasion assay showed the number of invasion cells was 36.66±1.53 in the overexpression group and 17.66±1.53 in the control group, and the difference was statistically significant(t=15.234, P<0.001). Annexin v-PI double staining assay showed the apoptosis rate was(2.75±0.58)% in the overexpression group and(4.81±0.31)% in the control group, and the difference was statistically significant(t=5.443, P=0.006). Conclusion miR-25-3p is highly expressed in endometrial cancer tissues. Overexpression of miR-25-3p promotes the proliferation, migration and invasion but inhibits the apoptosis of KLE cells.

Key words: miR-25-3p, Endometrial cancer, Proliferation, Migration, Invasion, Apoptosis

中图分类号:

R737.33

史丽,马静,赵喜娃,关英霞,赵连梅,单保恩. miR-25-3p在40例子宫内膜腺癌组织中的表达及对KLE细胞生物学功能的影响[J]. 山东大学学报 (医学版), 2020, 58(12): 86-91.

SHI Li, MA Jing, ZHAO Xiwa, GUAN Yingxia, ZHAO Lianmei, SHAN Baoen. Expression and function of miR-25-3p in endometrial cancer[J]. Journal of Shandong University (Health Sciences), 2020, 58(12): 86-91.

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