臭氧对大鼠星形胶质细胞作用的体外研究

山东大学学报(医学版)

臭氧对大鼠星形胶质细胞作用的体外研究

周乃宝，傅志俭，孙涛

山东大学附属省立医院疼痛科，济南 250021

收稿日期:2008-04-02 修回日期:1900-01-01 出版日期:2008-09-16 发布日期:2008-09-16
通讯作者: 傅志俭

Effects of ozone on rat astrocytes in vitro

ZHOU Nai-bao， FU Zhi-jian， SUN Tao

Department of Pain Management, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2008-04-02 Revised:1900-01-01 Online:2008-09-16 Published:2008-09-16
Contact: FU Zhi-jian

摘要/Abstract

摘要： 目的观察较短时间内臭氧(O3)对体外培养的大鼠星形胶质细胞(Ast)功能结构的影响，并探讨其作用机制。方法体外培养大鼠Ast并行免疫细胞化学鉴定；大鼠Ast传代接种入24孔培养板，分为3组（n=7），纯氧组（O₂组）加入400μL纯氧作用20min后的完全培养基；O₃40组、O₃80组分别加入400μL的40μg/mL、80μg/mL臭氧作用20min后的完全培养基，分别于2、4h收集细胞悬液及破碎细胞后简易测定法测定细胞乳酸脱氢酶（LDH）漏出率，台盼蓝染色计数死亡细胞百分比。结果与O₂组比较，O₃40组Ast LDH漏出率在2、4h均降低（P＜0.05），O380组在两时点均明显升高（P＜0.01），且O380组4h较2h升高明显（P＜0.05）；与O₂组比较，O₃80组Ast死亡细胞百分比在2、4h均升高（P＜0.01），且4h较2h升高明显（P＜0.01）。结论较短时间（2、4h）内，80μg/mL浓度的臭氧表现出对体外培养大鼠星形胶质细胞的损伤作用，而40μg/mL浓度的臭氧未表现出此作用。

关键词: Wistar, 乳酸脱氢酶, 大鼠, 星形细胞, 臭氧

Abstract: To explore the effects of ozone on astrocytes in vitro and to investigate its mechanism. MethodsAstrocytes were cultured in vitro in accordance with McCarthy′s method and were identified by immunohistochemistry staining. Then cells were seeded into 24well plates and divided into 3 groups(n=7): the oxygen group(O₂ group), the ozone 40(O₃40) group and the ozone 80(O₃80) group to which was respectively added 400μL complete medium(CM) acted by oxygen for 20min, 400μL CM acted by 40μg/mL ozone for 20min and 400μL CM acted by 80μg/mL ozone for 20min. After incubation for 2h or 4h, the lactate dehydrogenase (LDH) leaking ratio was determined by the simple method of Qingtao Hong and dead cell percentage was determined by trypan blue staining. ResultsThe LDH leaking ratio was decreased (P＜0.05) in the O₃40 group and was increased (P＜0.01) in the O₃80 group compared with the O₂ group, and also it was increased (P＜0.05) in the 4haction O₃80 group compared with the 2haction O₃ 80 group. Dead cell percentage in the O380 group was increased (P＜0.01) compared with the O₂ group and also it was increased (P＜0.05) in the 4haction group compared with the 2haction group. Conclusion 80mg/L ozone acting in the short term (2 or 4 hours) has a damage role on astrocytes, while 40mg/L ozone does not.

Key words: Ozone, Astrocyte, LDH leaking ratio, Dead cells′ percentage

中图分类号:

R614

周乃宝,傅志俭,孙涛. 臭氧对大鼠星形胶质细胞作用的体外研究[J]. 山东大学学报(医学版), 2008, 46(9): 863-865.

ZHOU Nai-bao,FU Zhi-jian,SUN Tao. Effects of ozone on rat astrocytes in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(9): 863-865.

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