姜黄挥发油对THP-1细胞增殖及凋亡的影响

doi:10.6040/j.issn.1671-7554.0.2014.731

山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (5): 46-51.doi: 10.6040/j.issn.1671-7554.0.2014.731

姜黄挥发油对THP-1细胞增殖及凋亡的影响

涂云华¹, 康颖倩², 周英³, 叶振源¹, 薛月萃¹, 邓仁远¹, 王梅竹², 陈兰¹, 曹煜¹

1. 贵阳医学院附院皮肤性病科, 贵州贵阳 550004;
2. 贵阳医学院微生物学教研室, 贵州贵阳 550004;
3. 贵州大学生命科学学院贵州省中药(民族药)创制工程中心, 贵州贵阳 550025

收稿日期:2014-10-21 修回日期:2014-12-16 出版日期:2015-05-10 发布日期:2015-05-10
通讯作者: 曹煜。E-mail:382541077@qq.com E-mail:382541077@qq.com
基金资助:
贵州省中药现代化专项项目[黔科合中药字(2012)5018号];贵阳市科技局科技创新平台项目[筑科合同(2012303)号]

Effect of turmeric volatile oil on the proliferation and apoptosis of human leukemia cell line THP-1

TU Yunhua¹, KANG Yingqian², ZHOU Ying³, YE Zhenyuan¹, XUE Yuecui¹, DENG Renyuan¹, WANG Meizhu², CHEN Lan¹, CAO Yu¹

1. Department of Dermatology and Sexually Transmitted Diseases, the Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou, China;
2. Department of Microbiology, Guiyang Medical College, Guiyang 550004, Guizhou, China;
3. Guizhou Province Chinese Medicine and Ethnic Medicine Creation Engineering Center, College of Life Sciences, Guizhou University, Guiyang 550025, Guizhou, China

Received:2014-10-21 Revised:2014-12-16 Online:2015-05-10 Published:2015-05-10

摘要/Abstract

摘要： 目的研究姜黄挥发油(TVO)对急性单核细胞白血病THP-1细胞株增殖及凋亡的影响,并探讨其作用机制。方法不同浓度(5~80 mg/L)TVO体外作用于THP-1细胞。应用噻唑蓝(MTT)法检测细胞增殖抑制率;经吉姆萨染色,倒置显微镜观察细胞形态变化;DNA片段化测细胞凋亡;流式细胞仪检测细胞凋亡及周期;比色法测Caspase-3酶活性。结果 TVO具有抑制THP-1细胞株增殖的作用,并表现出时间-剂量依赖性。同时,TVO能诱导THP-1细胞凋亡,呈现剂量依赖性,镜下见典型细胞凋亡形态,Caspase-3酶活性随药物浓度增加而增强。经TVO作用后的细胞周期被阻滞在G₁期,G₂/M期及S期细胞数减少。结论 TVO对THP-1细胞株具有增殖抑制及促凋亡的作用,其机制可能是使细胞周期阻滞在G₁期,且激活了细胞凋亡途径中关键酶Caspase-3活性,从而抑制肿瘤细胞的增殖与分化。

关键词: Caspase-3, THP-1细胞, 增殖, 细胞凋亡, 姜黄挥发油

Abstract: Objective To investigate the effect of turmeric volatile oil (TVO) on the apoptosis and proliferation of human leukemia cell line THP-1. Methods THP-1 Cells were incubated with different concentrations of TVO in vitro. The inhibition rate on cell proliferation was determined with methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. The change of morphology was observed with inverted microscope after Giemsa's staining. Apoptosis was evaluated with DNA fragmentation and flow cytometry (FCM); cell cycle was analyzed with FCM. Caspase-3 activity was measured with a colorimetric method. Results TVO exhibited obvious inhibitive effect on the growth of THP-1 cells in a time-dose dependent manner. Meanwhile, TVO induced apoptosis of THP-1 cells in a dose-dependent manner. Typical morphology changes of apoptosis were presented. Caspase-3 activity was enhanced with high concentration of TVO. After TVO treatment, the number of cells decreased in G₁/M phase and S phase but increased in G1 phase. Conclusion Turmeric volatile oil exhibits marked proliferation-inhibitive and apoptosis-inducing effect on THP-1 cells. The possible mechanism is to arrest cell cycle in G₁ phase, and activate the key enzyme of apoptosis pathway.

Key words: Proliferation, Turmeric volatile oil, Apoptosis, Caspase-3, THP-1 cells

中图分类号:

R932

涂云华, 康颖倩, 周英, 叶振源, 薛月萃, 邓仁远, 王梅竹, 陈兰, 曹煜. 姜黄挥发油对THP-1细胞增殖及凋亡的影响[J]. 山东大学学报（医学版）, 2015, 53(5): 46-51.

TU Yunhua, KANG Yingqian, ZHOU Ying, YE Zhenyuan, XUE Yuecui, DENG Renyuan, WANG Meizhu, CHEN Lan, CAO Yu. Effect of turmeric volatile oil on the proliferation and apoptosis of human leukemia cell line THP-1[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(5): 46-51.

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姜黄挥发油对THP-1细胞增殖及凋亡的影响

Effect of turmeric volatile oil on the proliferation and apoptosis of human leukemia cell line THP-1

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