尼莫地平对脑挫裂伤后神经细胞内游离钙浓度的影响

山东大学学报(医学版)

尼莫地平对脑挫裂伤后神经细胞内游离钙浓度的影响

郑志明,吴洪喜,滕良珠

山东大学附属省立医院神经外科，济南 250021

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-07-16 发布日期:2008-07-16
通讯作者: 滕良珠

Effects of nimodipine on intracellular free Ca2+ concentration and apoptosis in rat neural cells following cerebral contusions

ZHENG Zhi-ming, WU Hong-xi, TENG Liang-zhu

Department of Neurosurgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:1900-01-01 Revised:1900-01-01 Online:2008-07-16 Published:2008-07-16
Contact: TENG Liang-zhu

摘要/Abstract

摘要：

目的〓〖HTK〗研究尼莫地平对脑挫裂伤后神经细胞胞浆内游离钙离子浓度变化的影响。〖HTW〗方法〓〖HTK〗将Wistar大鼠随机分为对照组、创伤组、治疗组；创伤组按照Feeney自由落体脑损伤模型方式制作大鼠左顶叶脑挫裂伤模型，治疗组在外伤后开始静脉滴注尼莫地平。分别对脑挫裂伤后0.5、1、3、6、12、24、48h各时相点细胞内游离钙离子浓度和细胞凋亡率进行检测。对不同时相的创伤组、治疗组和对照组进行比较分析。〖HTW〗结果〓〖HTK〗脑挫裂伤后大脑皮层神经细胞内游离钙离子浓度迅速升高，0.5h时为正常值的4倍，24h时达到高峰，48h时降低明显，但仍然维持在比较高的水平。应用尼莫地平治疗后，钙离子浓度显著降低，6h时降至接近正常水平。脑挫裂伤后神经细胞凋亡率和胞内游离钙离子浓度存在相关性。〖HTW〗结论〓〖HTK〗脑挫裂伤后神经细胞胞浆内钙离子浓度升高，导致钙超载并引起细胞凋亡。早期应用尼莫地平能抑制钙超载，抑制细胞凋亡，对神经细胞具有极强的保护作用。

关键词: 脑损伤, 尼莫地平, 钙, 细胞凋亡

Abstract:

Objective〓〖WTBZ〗To study the effects of nimodipine on intracellular free Ca2+ concentration in rat neurocytes following cerebral contusions. 〖WTHZ〗Methods〓〖WTBZ〗Wistar rats were randomly divided into three groups: the control group, the trauma group and the treatment group. The cerebral contusion models were established based on the Feeney method and nimodipine was immediately administered after cerebral contusions. The related parameters at different times after cerebral contusion were detected. Intracellular free Ca2+ concentration and apoptosis rate were determined by flow cytometry. 〖WTHZ〗Results〓〖WTBZ〗After cerebral contusion, intracellular free Ca2+ concentration was increased and reached a peak at 24h and then gradually decreased, but it still maintained a high level at 48h, while nimodipine made the intracellular free Ca2+ concentration remarkably decrease and the Ca2+ level was decreased to the normal level at 6h (94.85±15.63nmol/L). 〖WTHZ〗Conclusion〓〖WTBZ〗Intracellular free Ca2+ concentration is related to apoptosis rate. Nimodipine can decrease the Ca2+ levels and has some protective role against cerebral contusion.

Key words: Apoptosis, Calcium, Brain injuries, Nimodipine

中图分类号:

R361+.3

郑志明,吴洪喜,滕良珠. 尼莫地平对脑挫裂伤后神经细胞内游离钙浓度的影响[J]. 山东大学学报(医学版), 2008, 46(7): 672-675.

ZHENG Zhi-ming,WU Hong-xi,TENG Liang-zhu.

Effects of nimodipine on intracellular free Ca2+ concentration and apoptosis in rat neural cells following cerebral contusions

[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(7): 672-675.

参考文献

相关文章 15

[1]	鹿向东杨伟徐广明曲元明. 脑膜瘤中PPAR-γ的表达及曲格列酮对脑膜瘤培养细胞生长的影响[J]. 山东大学学报(医学版), 2209, 47(6): 65-.
[2]	邢凯,郑强,孙金书,刘晓林,王正军. 人源瓣膜间质细胞体外成骨诱导方法比较[J]. 山东大学学报 (医学版), 2025, 63(6): 11-18.
[3]	李响,张艺,王雪纯,徐梦超,王月兰. 氧化应激在创伤性脑损伤诱发急性肺损伤中的研究进展[J]. 山东大学学报 (医学版), 2025, 63(2): 118-124.
[4]	姜子晗,芦兴晨,孙露,赵蕙琛,左丹,马小莉,刘元涛,张玉超. NR4A1通过IκBα/NF-κB通路调控过氧化氢诱导人脐静脉内皮细胞凋亡的机制[J]. 山东大学学报 (医学版), 2024, 62(3): 11-19.
[5]	吴雪燕,高英静,刘思言,王少莲. 以手足及面部麻木为首发表现的青少年孤立肾漏诊1例并文献复习[J]. 山东大学学报 (医学版), 2024, 62(3): 121-124.
[6]	曹华琳,贾彦召,曲莉,尹昕. CircFAT1调节miR-296-3p/MAPRE1轴对鼻咽癌细胞增殖、凋亡和放疗敏感性的影响[J]. 山东大学学报 (医学版), 2023, 61(9): 38-46.
[7]	刘金波,刘凯文,向崇鑫,程雷. 西红花苷对椎间盘退变的保护作用[J]. 山东大学学报 (医学版), 2023, 61(9): 84-93.
[8]	王荣荣,马晓庆. 成人急性B淋巴细胞白血病合并高钙危象1例并文献复习[J]. 山东大学学报 (医学版), 2023, 61(12): 100-106.
[9]	高辉香,宋阳. 特立帕肽治疗常染色体显性遗传低钙血症1例并文献复习[J]. 山东大学学报 (医学版), 2023, 61(11): 116-120.
[10]	杨元凤,熊高才,黎豫川,罗玉玲,张敬杰. 鹿苓安肾颗粒对慢性肾功能衰竭大鼠炎症反应及细胞凋亡的影响[J]. 山东大学学报 (医学版), 2023, 61(10): 9-16.
[11]	赵恩举,赵硕,郭云亮,王锡明. 282例颈动脉钙化与脑小血管病MRI总负荷评分的关联性[J]. 山东大学学报 (医学版), 2023, 61(1): 38-44.
[12]	曾彦平,闫圣涛,刘小瑜,江子晴. 以甲状旁腺危象为首发表现的甲状旁腺癌1例文献报道[J]. 山东大学学报 (医学版), 2023, 61(1): 62-68.
[13]	赵舸,邹存华,宋冬冬,赵淑萍. 丹参酮IIA对子宫内膜癌细胞增殖与凋亡的影响[J]. 山东大学学报 (医学版), 2022, 60(9): 53-58.
[14]	封海岗,刘国文,曹洪. 干扰MAD2L1基因表达对乳腺癌细胞凋亡的影响及机制[J]. 山东大学学报 (医学版), 2022, 60(10): 9-16.
[15]	章露文,范锐心,常颖,张晴. 微生态制剂肠内营养对43例重型颅脑损伤患者的疗效[J]. 山东大学学报 (医学版), 2021, 59(6): 111-116.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed