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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (4): 43-48.doi: 10.6040/j.issn.1671-7554.0.2014.927

• 基础医学 • 上一篇    下一篇

罗格列酮、胰岛素对Ⅱ型糖尿病大鼠脑内TIPE2的表达和细胞凋亡的影响

游洁冰1, 孙忠文2, 杜鹃3, 胥文娟4, 王雅琳1, 李帅1, 朱梅佳5   

  1. 1. 山东大学医学院, 山东 济南 250012;
    2. 烟台毓璜顶医院神经内科, 山东 烟台 264000;
    3. 山东大学附属千佛山医院医学研究中心, 山东 济南 250014;
    4. 济南军区总医院神经内科, 山东 济南 250031;
    5. 山东大学附属千佛山医院神经内科, 山东 济南 250014
  • 收稿日期:2014-12-09 修回日期:2015-02-28 出版日期:2015-04-10 发布日期:2015-04-10
  • 通讯作者: 朱梅佳.E-mail:zhumeijia1818@163.com E-mail:zhumeijia1818@163.com
  • 基金资助:
    山东省自然科学基金( ZR2010HM101,Y2008C124,ZR2009CL021);山东省科技攻关基金(2007GGWZ02056,2009GG10002024);济南市科技发展计划( 201202053).

Effects of rosiglitazone and insulin on the expression of TIPE2 and cell apoptosis in the brain of type 2 diabetic GK rats

YOU Jiebing1, SUN Zhongwen2, DU Juan3, XU Wenjuan4, WANG Yalin1, LI Shuai1, ZHU Meijia5   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Neurology, Yuhuangding Hospital of Yantai City, Yantai 264000, Shandong, China;
    3. Medical Research Center, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China;
    4. Department of Neurology, General Hospital of Jinan Military Area, Jinan 250031, China;
    5. Department of Neurology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China
  • Received:2014-12-09 Revised:2015-02-28 Online:2015-04-10 Published:2015-04-10

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摘要： 目的 探讨罗格列酮、胰岛素干预下Ⅱ型糖尿病大鼠脑内肿瘤坏死因子α诱导蛋白8样因子2(TIPE2)表达与细胞凋亡的变化,以及它们之间的相关性.方法 选取3月龄Wistar大鼠(n=5,3Wistar组)和3月龄GK大鼠(n=5,3GK组);4月龄GK大鼠喂养至6月龄,根据期间的不同处理分为6月龄GK大鼠(n=5,6GK组)、罗格列酮干预6月龄GK大鼠(n=5,RSG+6GK组)、胰岛素干预6月龄GK大鼠(n=5,INS+6GK组).采用免疫组化法检测各组大鼠脑内TIPE2的表达部位及特征,运用RT-PCR法和Western blotting法检测各组大鼠脑内TIPE2 mRNA和蛋白的表达,采用免疫荧光法检测各组大鼠脑内细胞凋亡的情况.结果 3GK组在染色强度、阳染细胞数、阳染血管数、mRNA、蛋白水平均较3 Wistar组有明显增加(P< 0.05),6GK组较3GK组有明显增加(P< 0.05),RSG+6GK组、INS+6GK组均较6GK组有明显减少(P< 0.05).大鼠脑内细胞凋亡数目与TIPE2的表达呈正相关性(r=0.9816,P< 0.05).结论 Ⅱ型糖尿病可促进大鼠脑内TIPE2的表达和细胞凋亡,随年龄的增长和糖尿病病程的进展, TIPE2的表达和细胞凋亡数目相应增加,罗格列酮、胰岛素干预可减少糖尿病大鼠脑内TIPE2的表达并抑制细胞凋亡.在糖尿病大鼠脑内细胞凋亡的进程中,TIPE2能够发挥促凋亡作用.

关键词: 肿瘤坏死因子&alpha, 骨折, 腰骶部, 糖尿病, PPAR&gamma, 细胞凋亡, 激动剂, 诱导蛋白8样因子2, 胰岛素, 髋部, 褥疮

Abstract: Objective To explore the effects of rosiglitazone and insulin on the expression of tumor necrosis factor-α induced protein 8 like-2 (TIPE2) and cell apoptosis in the brain of type 2 diabetic GK rats, and to investigate the mechanism involved. Methods A total of 25 rats were enrolled and divided into 5 groups, including 3-month-old Wistar rats in the 3Wistar group, 3-month-old GK rats in the 3GK group, 6-month-old GK rats in the 6GK group, rosiglitazone treated 6-month-old GK rats in the RSG+6GK group, and insulin treated 6-month-old GK rats in the INS+6GK group, with 5 rats in each group. Immunohistochemistry (IHC) was used to observe the quantity and features of TIPE2expression in different rats' brains. RT-PCR and Western blotting were adopted respectively to detect the mRNA and protein level of TIPE2 in different rats' brains. Immunofluorescence (IH) was applied to observe the numbers of apoptotic cells in different rats' brains. Results There were significant differences among the groups in the staining intensity, numbers of positively-stained cells and positively-stained vessels, level of mRNA and protein, and expression of TIPE2. The above indexes were highest in the 6GK group, followed by the 3GK group, 3Wistar group, RSG+6GK group and INS+6GK group (all P< 0.05). The number of apoptotic cells was positively associated with the expression of TIPE2 (r=0.9816, P< 0.05). Conclusion Type 2 diabetes can promote the expression of TIPE2 and cell apoptosis. With age and progression of diabetes mellitus, expression of TIPE2 and the number of apoptotic cells gradually increase. After rosiglitazone and insulin treatment, the expression of TIPE2 reduces and cell apoptosis is inhibited. In the course of diabetes mellitus, TIPE2 accelerates the cell apoptosis.

Key words: Tumor necrosis factor-&alpha, Diabetes mellitus, agonist, induced protein 8 like-2, Cell apoptosis, PPAR&gamma, Insulin

中图分类号: 

  • R741
[1] Sun H, Gong S, Carmody RJ, et al. TIPE2, a negative regulator of innate and adaptive immunity that maintains immune homeostasis[J]. Cell, 2008, 133(3):415-426.
[2] Freundt EC, Bidere N, Lenardo MJ, et al. A different TIPE of immune homeostasis[J]. Cell, 2008, 133(3):401-402.
[3] 孙忠文, 杜鹃, 鲍梦欣, 等. 2 型糖尿病大鼠脑组织TIPE2 的表达特征及其与Aβ1-40 沉积的相关性[J]. 山东大学学报:医学版, 2013, 51(3):11-14. SUN Zhongwen, DU Juan, BAO Mengxin, et al. Expression of TIPE2 and its association with Aβ1-40 deposition in brain tissue of type 2 diabetic GK rat[J]. Journal of Shandong University: Health Sciences, 2013, 51(3):11-14.
[4] Seed Ahmed M, Kovoor A, Nordman S, et al. Increased expression of adenylyl cyclase 3 in pancreatic islets and central nervous system of diabetic Goto-Kakizaki rats:a possible regulatory role in glucose homeostasis[J]. Islets, 2012, 4(5):343-348.
[5] Chan JC, Malik V, Jia W, et al. Diabetes in Asia:epidemiology, risk factors, and pathophysiology[J]. JAMA, 2009, 301(20):2129-2140.
[6] Jack M, Wright D. Role of advanced glycation endproducts and glyoxalase I in diabetic peripheral sensory neuropathy[J]. Transl Res, 2012, 159(5):355-365.
[7] Yamagishi S. Role of advanced glycation end products (AGE) and soluble receptor for AGE (sRAGE) in vascular complications in diabetes[J]. Nihon Rinsho, 2012, 70 (Suppl 5):243-247.
[8] Satoh J, Yagihashi S, Toyota T. The possible role of tumor necrosis factor-alpha in diabetic polyneuropathy[J]. Exp Diabesity Res, 2003, 4(2):65-71.
[9] Brands AMA, Kessels RPC, de Haan EHF, et al. Cerebral dysfunction in type 1 diabetes:effects of insulin, vascular risk factors and blood-glucose levels[J]. Eur J Pharmacol, 2004, 490(1-3):159-168.
[10] Kuhad A, Bishnoi M, Tiwari V, et al. Suppression of NF-kappabeta signaling pathway by tocotrienol can prevent diabetes associated cognitive deficits[J]. Pharmacol Biochem Behav, 2009, 92(2):251-259.
[11] Li A, Chen Y, Zhao X, et al. Characterization and transcriptional regulation analysis of the porcine TNFAIP8L2 gene[J]. Mol Genet Genomics, 2010, 284(3):185-195.
[12] Paravicini TM, Touyz RM. NADPH oxidases, reactive oxygen species, and hypertension:clinical implications and therapeutic possibilities[J]. Diabetes Care, 2008, 31 (Suppl 2):S170-180.
[13] Gill PS, Wilcox CS. NADPH oxidases in the kidney[J]. Antioxid Redox Signal, 2006, 8(9-10):1597-1607.
[14] Lee IT, Luo SF, Lee CW, et al. Overexpression of HO-1 protects against TNF-alpha-mediated airway inflammation by down-regulation of TNFR1-dependent oxidative stress[J]. Am J Pathol, 2009, 175(2): 519-532.
[15] Zhang S, Zhang Y, Wei X, et al. Expression and regulation of a novel identified TNFAIP8 family is associated with diabetic nephropathy[J]. Biochim Biophys Acta, 2010, 1802(11):1078-1086.
[16] Holm TH, Draeby D, Owens T. Microglia are required for astroglial Toll-like receptor 4 response and for optimal TLR2 and TLR3 response[J]. Glia, 2012, 60(4):630-638.
[17] Ekdahl CT, Kokaia Z, Lindvall O. Brain inflammation and adult neurogenesis:the dual role of microglia[J]. Neuroscience, 2009, 158(3):1021-1029.
[18] Jansson, PA. Endothelial dysfunction in insulin resistance and type 2 diabetes[J]. J Intern Med, 2007, 262(2):173-183.
[19] Madonna R, Massaro M, De Caterina R. Insulin potentiates cytokine-induced VCAM-1 expression in human endothelial cells[J]. Biochim Biophys Acta, 2008, 1782(9):511-516.
[20] Daynes, RA, Jones DC. Emerging roles of PPARs in inflammation and immunity[J]. Nat Rev Immunol, 2002, 2(10):748-759.
[21] Dandona P, Ghanim H, Bandyopadhyay A, et al. Insulin suppresses endotoxin-induced oxidative, nitrosative, and inflammatory stress in humans[J]. Diabetes Care, 2010, 33(11):2416-2423.
[22] Eizirik DL, Millard I. Chronicle of a death foretold:endoplasmic reticulum stress and beta cell apoptosis in diabetes[J]. Med Sci (Paris), 2014, 30(5):496-499.
[23] Scatena R, Bottoni P, Botta G, et a1. The role of mitochondria in pharmacotoxicology:a reevaluation of an old, newly emerging topic[J]. Am J Physiol Cell Physiol, 2007, 293(1):12-21.
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