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山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (12): 30-34.doi: 10.6040/j.issn.1671-7554.0.2014.688

• 基础医学 • 上一篇    下一篇

雌激素及顺铂对卵巢癌细胞A2780PADI4的表达及其细胞增殖的影响

周静1,2, 郑亚冰2, 闫莉2, 常晓天2, 赵珊2, 荣风年2   

  1. 1. 山东大学医学院, 山东 济南 250012;
    2. 山东大学附属千佛山医院妇产科, 山东 济南 250014
  • 收稿日期:2014-10-10 修回日期:2014-11-11 发布日期:2014-12-10
  • 通讯作者: 荣风年。E-mail:fnrong@163.com E-mail:fnrong@163.com
  • 基金资助:
    山东省自然科学基金(ZR2013HM037)

Effects of estrogen and cis-platinum on PADI4 expression and cell proliferation in ovarian cancer cell line A2780

ZHOU Jing1,2, ZHENG Yabing2, YAN Li2, CHANG Xiaotian2, ZHAO Shan2, RONG Fengnian2   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Gynecology and Obstetrics, Qianfoshan Hospital of Shandong University, Jinan 250014, Shandong, China
  • Received:2014-10-10 Revised:2014-11-11 Published:2014-12-10

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摘要： 目的 探讨雌激素及顺铂对卵巢癌细胞A2780中肽酰基精氨酸脱亚胺酶4(PADI4)表达的作用及其对癌细胞增殖的影响。方法 在培养的卵巢癌细胞系A2780细胞中加入不同浓度17β-雌激素作用96 h后(10-12、10-10、10-8、10-6、10-4 mmol/L),采用实时定量 PCR及Western Blotting检测PADI4表达,并用CCK-8试剂盒检测细胞增殖,选取PADI4的表达变化最明显的雌激素浓度,将A2780细胞分为空白对照组、雌激素组、顺铂组、雌激素加顺铂组(联合组),采用上述方法检测各组PADI4的表达和细胞的增殖情况。结果 ①雌激素作用96 h后PADI4表达及细胞增殖随雌激素浓度升高而逐渐降低,但当雌激素浓度升至10-4mmol/L 时PADI4表达及细胞增殖率开始升高。②与空白对照组比,联合组和顺铂组培养96 h后PADI4表达均明显降低,且联合组细胞增殖率较雌激素组低。结论 雌激素可随浓度不同调控卵巢癌细胞A2780中PADI4的表达和细胞增殖;雌激素对卵巢癌细胞增殖的影响可能与PADI4表达有关。

关键词: 细胞增殖, 顺铂, 肽酰基精氨酸脱亚胺酶4, 17β-雌激素, 卵巢肿瘤

Abstract: Objective To investigate the effects of estrogen and cis-platinum on PADI4 expression and cell proliferation in ovarian cancer cell line A2780. Methods A2780 cells were cultured in medium with estrogen (10-12, 10-10, 10-8, 10-6, 10-4mmol/L). The expression of PADI4 was detected using real-time PCR and Western blotting after 96 hours. The cell proliferation was measured with CCK-8 assay. The concentration of estradiol-17β was chosen at which PADI4 expression changed the most significantly. A2780 cells were grouped into blank control group,single-agent estradiol-17β group, single-agent cis-platinum group and estradiol-17β plus cis-platinum group (combination group). The PADI4 expression and cell proliferation were measured by real-time PCR, Western blotting and CCK-8 assay. Results ① The PADI4 expression and cell proliferation decreased in A2780 cells treated with estradiol-17β in the range of 10-12mmol to 10-6mmol, but increased with higher concentration of estradiol-17β. A significant association was detected between PADI4 expression and cell proliferation. ② Compared with the blank control group, PADI4 expression decreased in cells cultured with estradiol-17β plus cis-platinum and single-agent cis-platinum group, and the proliferation of combination group was lower than that of the single-agent cis-platinum group. Conclusion Estradiol-17β regulates the expression of PADI4 and cell proliferation of ovarian cancer. The role of estradiol-17β may be associated with PADI4 expression.

Key words: Ovarian tumor, Peptidylarginine deiminase 4, Estradiol-17β, Cis-platinum, Cell proliferation

中图分类号: 

  • R711.75
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