利鲁唑对急性高眼压致大鼠视网膜损伤的保护作用

doi:10.6040/j.issn.1671-7554.0.2015.238

摘要/Abstract

摘要： 目的探讨腹腔内注射利鲁唑对急性高眼压所致的大鼠视网膜损伤的保护作用.方法将120只大鼠分为正常对照组、高眼压对照组及利鲁唑干预组,每组40只.采用前房灌注的方式建立急性高眼压模型,免疫组化及末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记法(TUNEL) 分别测定3组大鼠再灌注后12、24、48、72 h的Caspase-3蛋白表达情况及大鼠视网膜各层细胞的凋亡水平,HE染色观察各组大鼠视网膜结构改变.结果与高眼压对照组相比,利鲁唑干预组视网膜增厚及结构紊乱情况明显减轻,不同时间点Caspase-3的表达水平及凋亡细胞数明显降低(P< 0.05).结论利鲁唑可减少大鼠急性高眼压损伤后视网膜神经节细胞凋亡,对视网膜具有一定的保护作用.

关键词: Caspase-3, 急性高眼压, 凋亡, 利鲁唑

Abstract: Objective To investigate the protective effect of riluzole on the damage of rats' retinal ganglion cells induced by acute intraocular hypertension. Methods A total of 120 SD rats were randomly divided into control group, high intraocular pressure group, and riluzole intervention group, 40 in each group. Acute ocular hypertention rats model, which were established using the anterior chamber perfusion way, were injected intraperitoneally with riluzole. After 12, 24, 48 and 72 h of reperfusion, the expression of Caspase-3 protein and the levels of apoptosis in each layer of rats' retinal cells were detected by immunohistochemistry and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL). The structural changes of the rats' retina in each group were observed by HE staining. Results Compared with the high intraocular pressure group, the thickening and structural disorder in riluzole intervention group were relieved, and the expression of Caspase-3 and the apoptosis cells at different time points in riluzole intervention group decreased (P< 0.05). Conclusion Riluzole can reduce the apoptosis of retinal cells in rats with damaged retina caused by acute ocular hypertention and has significant protective effects to retina.

Key words: Riluzole, Apoptosis, Caspase-3, Acute ocular hypertension

中图分类号:

R574

张楠, 周利晓, 孙成林. 利鲁唑对急性高眼压致大鼠视网膜损伤的保护作用[J]. 山东大学学报（医学版）, 2015, 53(8): 27-31.

ZHANG Nan, ZHOU Lixiao, SUN Chenglin. Protective effect of riluzole on the damage of rat retina induced by acute intraocular hypertension[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(8): 27-31.

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