酶酚酸酯对实验性自身免疫性脑脊髓炎大鼠神经功能的影响

山东大学学报(医学版)

酶酚酸酯对实验性自身免疫性脑脊髓炎大鼠神经功能的影响

由玮，毕建忠，谢兆宏，朱正禹，王晓云

山东大学第二医院神经内科，济南 250033

收稿日期:2007-12-11 修回日期:1900-01-01 出版日期:2008-06-16 发布日期:2008-06-16
通讯作者: 毕建忠

Effects of mycophenolate mofetil on neural function of experimental autoimmune encephalomyelitis in rats

YOU Wei, BI Jian-zhong， XIE Zhao-hong， ZHU Zheng-yu， WANG Xiao-yun

Department of Neurology, Second Hospital of Shandong University, Jinan 250033, China

Received:2007-12-11 Revised:1900-01-01 Online:2008-06-16 Published:2008-06-16
Contact: BI Jian-zhong

摘要/Abstract

摘要： 目的观察酶酚酸酯(MMF)对实验性自身免疫性脑脊髓炎(EAE)大鼠神经功能改善及脑脊髓组织的影响。方法用豚鼠全脊髓匀浆和完全弗氏佐剂制成的完全抗原免疫Wistar大鼠，制备EAE大鼠模型，以生理盐水和完全弗氏佐剂注射的Wistar大鼠作为对照。对照组大鼠7只，造模成功且存活的大鼠为25只，随机分为MMF大剂量组（n=8只）,MMF小剂量组（n=8只）,模型组（n=9只）。连续给药14d，每天观察记录神经功能评分及体重变化，14d后HE染色观察脑、脊髓组织病理变化。结果EAE大鼠的造模成功率为72.1%。与对照组相比，各组EAE大鼠神经功能评分明显增加，体重明显下降(P＜0.01)，脑脊髓组织病理学变化主要表现为血管周围有大量炎细胞浸润；与模型组相比，用MMF治疗后神经功能评分有所下降，体重增加，病理表现有不同程度的减轻，且大剂量MMF疗效优于小剂量MMF(P＜0.01)。结论MMF能改善EAE大鼠的症状，促进神经功能恢复，并具有剂量依赖性。

关键词: 大鼠, Wistar, 自身免疫性, 脑脊髓炎, 多发性硬化, 酶酚酸酯

Abstract: To explore the effects of mycophenolate mofetil on the improvement of neural function and brain and spine cord of rats with experimental autoimmune encephalomyelitis (EAE). MethodsWistar rats were induced by guinea pig spinal cord homogenate and complete freund adjuvant to establish EAE models, while the control rats were induced by saline and complete freund adjuvant. There were 7 control rats and 25 EAE rats which were successfully made and survived. The EAE rats were randomly divided into three groups: the high dose of mycophenolate mofetil treatment group（n=8）, the low dose of mycophenolate mofetil treatment group（n=8）, and the model group（n=9）. Medicine was administered for 14 days and then the Duckers score and weight changes were assessed every day. After 14 days, pathological changes of brain and spine cord were observed by Hematoxylin and Eosin staining. ResultsThe achievement ratio of the EAE model was 72.1%. Compared with the control group, the Duckers score of the EAE rats increased with a decrease of weight(P＜0.01), pathological changes manifested inflammatory cells infiltration around vessels with HE staining. Compared with the model group, after treatment with mycophenolate mofetil, the Duckers score of the EAE rats decreased with an increase of weight and pathological changes lessened, moreover， the therapeutic effect of high dose mycophenolate mofetil was superior to low dose mycophenolate mofetil(P＜0.01). ConclusionMycophenolate mofetil can improve the symptoms of EAE rats and promotes the recovery of neural function in a dosedependent manner.

Key words: Mycophenolate mofetil, Multiple sclerosis, Autoimmune, Encephalomyelitis, Rats, Wistar

中图分类号:

R744.5

由玮,毕建忠,谢兆宏,朱正禹,王晓云. 酶酚酸酯对实验性自身免疫性脑脊髓炎大鼠神经功能的影响[J]. 山东大学学报(医学版), 2008, 46(6): 574-578.

YOU Wei,BI Jian-zhong,XIE Zhao-hong,ZHU Zheng-yu,WANG Xiao-yun. Effects of mycophenolate mofetil on neural function of experimental autoimmune encephalomyelitis in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(6): 574-578.

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