脑神康胶囊对缺氧复氧损伤大鼠海马神经元凋亡的影响

山东大学学报(医学版)

脑神康胶囊对缺氧复氧损伤大鼠海马
神经元凋亡的影响

李伟，刘德山，常萍，林炜炜

（山东大学齐鲁医院中医科，济南 250012）

收稿日期:1900-01-01 修回日期:1900-01-01 发布日期:2009-04-16
通讯作者: 刘德山

Effect of Naoshenkang Capsule on neuronal apoptosis and expression of Bcl2, Bax mRNA in cultured rat hippocampal neurons with anoxiareoxygenation injury

LI Wei, LIU Deshan, CHANG Ping， LIN Weiwei

（Department of Traditional Chinese Medicine, Qilu Hospital of Shandong University, Jinan 250012, China）

Received:1900-01-01 Revised:1900-01-01 Published:2009-04-16
Contact: LIU Deshan

摘要/Abstract

摘要： 目的观察脑神康胶囊对缺氧复氧损伤大鼠海马神经元凋亡及其Bcl2、Bax mRNA表达的影响。方法随机将原代培养大鼠海马神经元分为对照组、损伤组、中药低、中、高浓度组。对照组正常培养，损伤组及中药组建立缺氧复氧损伤模型，中药各浓度组于复氧时换以不同浓度含药血清的培养液，采用MTT法测各组细胞存活率，采用流式细胞仪检测凋亡率，采用RTPCR法检测Bcl2和Bax mRNA的表达并计算两者比值。结果损伤组细胞存活率及Bcl2 mRNA表达明显下降(P均＜0.01)，凋亡率及Bax mRNA表达显著升高(P均＜0.01)，Bcl2/Bax降低(P＜0.01)；中药各浓度组较损伤组细胞存活率及Bcl2 mRNA表达均升高(P均＜0.01)，凋亡率及Bax mRNA表达降低(P＜0.01，P＜0.05)，Bcl2/Bax增加(P＜0.01)，且呈明显的剂量依赖性。结论脑神康胶囊对海马神经元缺氧复氧损伤有明显的保护作用,其作用机制可能与其调节凋亡相关蛋白、减少凋亡密切相关。

关键词: 海马, 神经元, 再灌注损伤, 细胞凋亡, 基因,bcl2, 脑神康胶囊, 大鼠,Wistar

Abstract: To explore the effect of Naoshenkang Capsule, a traditional Chinese drug with the function of invigorating Qi and tonifying kidneys on neuronal apoptosis and expression of Bcl2, Bax mRNA in cultured rat hippocampal neurons with anoxiareoxygenation injury. MethodsPrimarily cultured neonate rat hippocampal neurons were randomly divided into five groups: the control group, the anoxiareoxygenation group and the small, middle, and large dosage of Chinese Medicineprotective groups. The Chinese Medicineprotective groups were treated with different concentrations of Naoshenkang containing serum before reoxygenation. The survival rate and apoptosis rate of neurons were determined by MTT and flow cytometry. RTPCR was used to evaluate mRNA expressions of Bcl2 and Bax genes. ResultsSurvival rate of cells, Bcl2 mRNA expression and Bcl2/Bax in the anoxiareoxygenation group were significantly lower (all P＜0.01), but apoptosis rate and Bax mRNA expression were higher (all P＜0.01) than those in the control group. Survival rate of cells, Bcl2 mRNA expression and Bcl2/Bax in all Chinese Medicineprotective groups were obviously higher (all P＜0.01), but apoptosis rate and Bax mRNA expression were lower (P＜0.01, P＜0.05) than those in the anoxiareoxygenation group and demonstrated obvious dosageeffective relationship. ConclusionNaoshenkang Capsule has a protective effect on hippocampal neurons against anoxiareoxygenation injury, and its mechanism may be that the Chinese medicine can regulate protein expressions of Bcl2 and Bax and inhibits apoptosis.

Key words: Hippocampus, Neurons, Reperfusion injury, Apoptosis, Genes,bcl2, Naoshenkang Capsule, Rats,Wistar

中图分类号:

R285.5

李伟，刘德山，常萍，林炜炜
. 脑神康胶囊对缺氧复氧损伤大鼠海马
神经元凋亡的影响[J]. 山东大学学报(医学版), 2009, 47(04): 26-29.

LI Wei, LIU Deshan, CHANG Ping， LIN Weiwei
.

Effect of Naoshenkang Capsule on neuronal apoptosis and expression of Bcl2, Bax mRNA in cultured rat hippocampal neurons with anoxiareoxygenation injury

[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(04): 26-29.

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