罗格列酮对高脂饲养大鼠骨骼肌AMPK活性的影响

山东大学学报(医学版)

罗格列酮对高脂饲养大鼠骨骼肌AMPK活性的影响

孙颖^1，2，刘毅³，张捷²，崔彬²，孔磊¹，赵家军¹

（山东大学附属省立医院 1.内分泌科，2.科研中心，3.呼吸内科，济南 250021）

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-03-16 发布日期:2009-03-16
通讯作者: 赵家军

Effects of Rosiglitazone on activities of AMPK
in the skeletal muscle of highfat fed rats

SUN Ying^1，2， LIU Yi³，　ZHANG Jie²，　CUI Bin²，　KONG Lei¹，　ZHAO Jiajun¹

(1. Department of Endocrinology； 2. Central Laboratory； 3. Department of
Respiratory, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-03-16 Published:2009-03-16
Contact: ZHAO Jiajun

摘要/Abstract

摘要： 目的观察罗格列酮对高脂饲养大鼠骨骼肌AMP激活的蛋白激酶(AMPK）α、葡萄糖转运体4(GLUT4）、胰岛素受体底物1(IRS1）及过氧化物酶体增殖物激活受体γ(PPARγ）表达的影响，为临床有效的预防和控制脂毒性提供科学依据。方法雄性Wistar大鼠30只，随机分为对照、高脂和高脂加罗格列酮［1?mg/(kg·d)］三组，每组10只。喂养5个月后，采用Real time PCR法测定大鼠骨骼肌中AMPKα1、AMPKα2、GLUT4的mRNA水平，RTPCR法测定IRS1、PPARγmRNA的表达，Western blot法测定PAMPK、TAMPK和GLUT4的含量。结果高脂组大鼠除AMPKα1和IRS1的mRNA表达无变化外，AMPKα2、 GLUT4、PPARγ的mRNA水平及PAMPK、TAMPK、 GLUT4的蛋白水平均比对照组降低（P<0.01或P<0.05）。与高脂组相比，罗格列酮显著增加大鼠骨骼肌GLUT4、PPARγ、IRS1的mRNA水平及 PAMPK、GLUT4的蛋白水平（P<0.01或P<0.05）, 而对AMPKα1、AMPKα2的mRNA水平和TAMPK的蛋白水平无明显影响。结论罗格列酮可增加高脂喂养大鼠的骨骼肌PAMPK、GLUT4、PPARγ和IRS1的表达，提示其具有参与改善胰岛素抵抗、缓解脂毒性的作用。

关键词: 罗格列酮, 蛋白激酶,AMP激活, 高脂, 大鼠,Wistar

Abstract: To investigate effects of Rosiglitazone on activities of AMPK (AMPactivated protein kinase)，GLUT4 (glucose transport 4)，IRS1 (insulin receptor substrate 1)， and PPARγ (peroxisome proliferator activated receptor γ) in the skeletal muscle of highfat fed rats. MethodsMale Wistar rats were randomly divided into three groups：the control group (N, n=10), the highfat diet group (HF, n=10), and the Rosiglitazonetreated group (HF+Ros，n=10). After a high fat feeding for four months, rats in the HF+Ros group were treated with Rosiglitazone at a dose of 1?mg/(kg·d) orally for one month. Skeletal muscles of all animals were gained for further analysis. The mRNA levels of AMPKα1, AMPKα2, and GLUT4 were determined using real time PCR and of IRS1 and PPARγ using RTPCR. Protein levels of total AMPK, PAMPK, and GLUT4 were determined by Western blot. ResultsCompared with the controls, the mRNA levels of AMPKα2, GLUT4, and PPARγ were reduced, and also protein expressions of total AMPK, PAMPK and GLUT4 were decreased in rats with a highfat diet. But the AMPKα1 and IRS1 mRNA levels had no changes. Compared with the HF group, Rosiglitazone increased mRNA levels of GLUT4, PPARγ and IRS1 and enhanced protein expressions of PAMPK and GLUT4. But there were no changes in AMPKα1, AMPKα2, and TAMPK. ConclusionRosiglitazone could increase expression of PAMPK, GLUT4, PPARγ, and IRS1 in highfat fed rats, which suggests that it could ameliorate insulin resistance.

Key words: Rosiglitazone, AMPactivated protein kinase, Highfat, Rats，Wistar

中图分类号:

孙颖1，2 ，刘毅3 ，张捷2 ，崔彬2 ，孔磊1 ，赵家军1
. 罗格列酮对高脂饲养大鼠骨骼肌AMPK活性的影响[J]. 山东大学学报(医学版), 2009, 47(03): 48-52.

SUN Ying^1，2， LIU Yi³，　ZHANG Jie²，　CUI Bin²，　KONG Lei¹，　ZHAO Jiajun¹

Effects of Rosiglitazone on activities of AMPK
in the skeletal muscle of highfat fed rats

[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(03): 48-52.

参考文献

相关文章 15

[1]	袁孟绮,霍凤蕾,任会萍,郭秋爽,蓝菁. Sdccag3通过Wnt通路对高脂血症大鼠种植体骨结合的影响[J]. 山东大学学报 (医学版), 2022, 60(7): 66-73.
[2]	龙宇晗,罗霞,姜洁. 妊娠期异常高脂血症2例报告并文献复习[J]. 山东大学学报 (医学版), 2021, 59(3): 103-106.
[3]	张霁娟,于汉成,王蓝,陈诺,崔书萌,高希宝. 高脂膳食、硒对大鼠抗氧化功能的影响[J]. 山东大学学报 (医学版), 2021, 59(1): 95-101.
[4]	李艳,孙凤娇,张天然,王雨心,张正铎,高希宝. 高糖、高脂饮食与不同浓度硒对大鼠脂代谢及氧化应激的影响[J]. 山东大学学报 (医学版), 2020, 58(5): 98-106.
[5]	李源,王强,石晓蕾,张晓丽,徐欣. 卷曲受体蛋白4在高脂血症大鼠种植体周围组织中的早期表达[J]. 山东大学学报 (医学版), 2020, 58(2): 29-35.
[6]	尹妮,杨关林,姜钧文,王春田,王凤耀,贾连群,高晓宇,潘嘉祥,李芹,李佳,冯元洁,高玉竹,周鹤,张哲. 巴马小型猪冠状动脉粥样硬化模型的评价方法[J]. 山东大学学报（医学版）, 2017, 55(7): 1-5.
[7]	张光,王广银,吴红彦, 张红玉,王停停,李吉庆,李敏,康凤玲,刘言训,薛付忠. 健康管理人群高脂血症风险预测模型[J]. 山东大学学报（医学版）, 2017, 55(6): 72-76.
[8]	沙莎,Chowdhury Sumon Rahman,陈丽,何芹,张婧,范珊珊,孙磊. 利拉鲁肽对高脂诱导肥胖小鼠瘦素、脂肪分布及含量的影响[J]. 山东大学学报（医学版）, 2016, 54(8): 22-27.
[9]	李志爽,于琼,张春华. 罗格列酮与GW9662对人绒癌JEG-3细胞中PPAR-γ表达的影响[J]. 山东大学学报（医学版）, 2016, 54(3): 72-76.
[10]	姚志娜, 韩婷, 范秀玲, 章由生, 宋佳伦. 胰高血糖素样肽-1对高脂饲养大鼠卵巢生物钟节律的影响[J]. 山东大学学报（医学版）, 2015, 53(2): 19-21.
[11]	容春莉, 姚文静, 杨倩, 李如意, 曹东平, 刘美霞, 彭应心. 阿托伐他汀钙联合依折麦布治疗冠状动脉粥样硬化并高脂血症的临床疗效[J]. 山东大学学报（医学版）, 2015, 53(11): 46-49.
[12]	褚倩倩1，许刚2，任桂杰1，徐霞1，胡中一1，刘永青1，苑辉卿1，田克立1. 过氧化物酶体增殖物激活受体γ激动剂对增强人结肠癌化疗敏感性的作用[J]. 山东大学学报(医学版), 2012, 50(1): 66-71.
[13]	车筱琪,李茵茵,王哲,陈海燕,周洁. 改善胰岛素抵抗对2型糖尿病患者胰高血糖素样肽-1水平的影响[J]. 山东大学学报(医学版), 2011, 49(6): 5-7.
[14]	张敏1，2，徐贵发1. 单纯高脂血症患者非药物干预的疗效评价[J]. 山东大学学报(医学版), 2010, 48(8): 117-119.
[15]	祖增艳1，张贵宇1，马本红1，王红霞2. 罗格列酮联合顺铂对子宫内膜癌裸鼠移植瘤生长的抑制作用[J]. 山东大学学报(医学版), 2010, 48(5): 62-66.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed