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Table of Content

      
    16 June 2008
    Volume 46 Issue 6
    Articles
    Articles
    Establishment of RAW264.7-T4 cell line with stable and high expression of murine Tim-4
    XU Li-yun,QI Jian-ni,LIANG Xiao-hong,JU Ying,SUN Wen-sheng,ZHANG Li-ning,ZHAO Pei-qing,GAO Li-fen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  551-555. 
    Abstract ( 1169 )   PDF (647KB) ( 314 )   Save
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    To establish a new macrophage cell line of murine Tim-4. MethodsPeritoneal macrophages were regularly separated from mice. RT-PCR was used to amplify the full gene fragment of murine Tim-4,with which to construct the pcDNA3.0Tim-4 expression vector. Restriction endonucluease digestion and sequencing were used to verify its correctness. Also, RAW264.7 cells were separately tansfected with pcDNA3.0 and pcDNA3.0-Tim-4 by liposomes. After screening with a high level of G418, a new cell line expressing stable and high Tim-4 was established. The mRNA and protein levels were further determined by RT-PCR, FCM and immunochemistry staining. ResultsProducts of RT-PCR amplification were digested with BamHⅠand HindⅢ，then Tim-4 was cloned into pcDNA3.0 and pcDNA3.0-Tim-4 recombinant was constructed. The result was consistent with the sequence of GenBank after digestion and sequencing. The mRNA level of RAW264.7-Tim-4 was significantly higher than that of the control group, as were proteins expressed in these cells, which was shown by FCM and immunochemistry staining. Also, Tim-4 expression was mainly found on the surface of the macrophage and cytoplasmic expression was also found. ConclusionAn expression vector with the full murine Tim-4 gene and a new line with stable and high expression of Tim-4 were successfully established, which will set a good basis for further study on the interaction of Tim-1 and Tim-4 and its impact on immunocytes.
    ERCC1 expression and cisplatin based adjuvant chemotherapy in non-small cell lung cancer
    WU Ming-sheng,WANG Ping,LIN Jun-hao,WANG Qian,WU Shu-ming
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  556-560. 
    Abstract ( 1444 )   PDF (469KB) ( 208 )   Save
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    To explore the relationship between excision repair crosscomplementation group 1 (ERCC1) protein expression and clinical outcome of cis-platinum based adjuvant chemotherapy in non small cell lung cancer (NSCLC), in order to understand the possible mechanism of cisplatinum resistance in NSCLC and provide predictive bio-markers to tailor adjuvant chemotherapy regimens and improve survival and response in NSCLC patients. Methods We examined the ERCC1 expression in 112 NSCLC patients by immunohistochemical staining of paraffin embedded tumor tissues, analyzed the relationship between the ERCC1 expression and NSCLC patients′ age, pathology category, TNM stage, chemotherapy and survival, especially focusing on the relationship between the ERCC1 expression and adjuvant cis-platinum based chemotherapy and survival of NSCLC patients. ResultsIn NSCLC patients with positive ERCC1 protein expression, the survival of those who received adjuvant cis-platinum chemotherapy was significantly shorter than that of those did not receive adjuvant cis-platinum chemotherapy (P＜0.01); in NSCLC patients with negative ERCC1 expression, there were no significant differences between the survival of those with and without adjuvant cisplatin chemotherapy (P＞0.05), but there were trends that the former was longer than the latter. After excluding ERCC1 expression, survival was not related to patient age (χ2=1.008, P=0.297), but related to TNM stages; the earlier the TNM stages, the longer the survival (χ2=51.326, P=0.000); also it was related to pathological categories: the survival of adenocarcinoma was longer than that of squamous cell carcinoma (χ2=6.339, P=0.012). In all subgroups (patient age, TNM stage, pathology category), the survival of ERCC1 positive patients was longer than that of ERCC1 negative patients. Conclusion1. Cis-platinum chemotherapy may benefit the NSCLC patient with negative expression of ERCC1. 2. Positive ERCC1 expression indicates cisplatinum resistance, so ERCC1 may be used as a predictive bio-marker to tailor adjuvant chemotherapy. 3. ERCC1 protein expression might be an important index for prognosis.
    Effects of the siRNA silencing heparanase gene on T24 cells′proliferation and MMP-2 expression in human bladder cancer
    ZHANG Kai-shu,XU Zhong-hua,JIANG Hong,YAN Lei,ZHOU Zun-lin,WANG Rong,LIU Hai-nan,FAN Yi-dong,LIU Zhao-xu,ZHENG Bao-zhong,QI Tian-wei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  561-565. 
    Abstract ( 1057 )   PDF (528KB) ( 498 )   Save
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    To explore the changes of proliferation and MMP-2 expression in T24 cells after siRNA downregulating heparanase (Hpa) gene on bladder cancer cell line-T24. MethodsCells were divided into six groups: the normal control group, the blank control group, the negative control group, the Hpa-siRNAⅠ group, the Hpa-siRNAⅡ group, and the Hpa-siRNAⅢ group. Chemically synthesized small interfering RNA (siRNA) silencing Hpa gene was transfected into the human bladder cancer cell line-T24. Then proliferation of T24 cells was determined by MTT assay, expression of Hpa mRNA was determined by reverse transcription polymerase chain reaction (RT-PCR), and expression of MMP-2 was determined by immunocytochemistry. ResultsMTT showed that the growth of T24 cells was decreased by (28.55±3.66)%, (27.20±3.25)% and (37.29±4.82)% in the three experimental groups. RT-PCR analyses also showed that the Hpa mRNA level was significantly decreased in some experimental groups compared with the control groups. Also immunocytochemical staining showed that the MMP-2 (Gelatinase A) expressions were much lower in the experimental group than those in the control groups(P＜0.05). Conclusions RNA interference (RNAi) could significantly decrease T24 cell proliferation and the Hpa gene mRNA level, and inhibits the MMP-2 expression in the bladder cancer cell line-T24.
    Medication intervention on heme oxygenase/carbon monoxide and nitric oxide synthase/nitric oxide systems in the atherosclerosis model of rabbits
    LIU Tong-tao,YU Qing-xia,TIAN Qing-yin,SUN Chun-li,LI Bo-qin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  566-570. 
    Abstract ( 1148 )   PDF (562KB) ( 602 )   Save
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    To investigate changes and intercorrelation of heme oxygenase-1(HO-1)/carbon monoxide(CO) and nitric oxide synthase (NOS)/nitric oxide(NO) and the effect of simvastatin on them in atherosclerotic progress. Methods16 rabbits had a 1% cholesterol diet for eight weeks and after eight weeks were fed with normal diets for eight weeks and then were divided into two groups: the simvastatin group and the model group, 8 rats fed with normal diets served as the control group. Levels of serum lipids, serum NO and plasma CO were determined at the beginning, the 8th week and the 16th week, and also the expressions of HO-1 and NOS in the thoracia aortic tissues were determined by the immunohistochemistry technique. ResultsAt the end of the 8th week, levels of serum lipids and plasma CO were increased in the simvastatin group (P＜0.01) and the level of serum NO and expressions of HO-1 and inducible nitric oxide synthase (iNOS) were decreased compared with the control group (P＜0.01). At the end of the 16th week, levels of serum lipids and plasma CO and expressions of HO-1 and iNOS were significantly decreased (P＜0.01), with the model group. Conclusions In atherosclerotic progress, HO1/CO and NOS/NO systems have a reciprocal relationship, and simvastatin may delay the atherosclerotic progress by decreasing the two systems.
    Expression of P-selectin deprivation from the platelets of asthmatic BALB/c mice
    WANG LI-sheng,ZHANG Chun-ling,SUN Rong-li,LI Li,XIAO Bao-hong,WU Xiao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  571-573. 
    Abstract ( 1142 )   PDF (476KB) ( 254 )   Save
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    To explore the expression of P-selectin derived from platelets of asthmatic mice. Methods Female BABL/c mice were randomly divided into two groups: the control group and the asthma group, and the expression of P-selectin was determined by flow cytometer (FCM). ResultsPositive expression of P-selectin rates in the control group and the asthma group were（1．23±1.02）% and （4.52±1.45）%（P<0.01）, but after thrombin activation, they were（45.12±4.03）% and（44.68±5.34）% (P>0.05). The leukocyte counts of bronchial alveo lavage(BAL) in the control group and the asthma group were 6.64±1.86 and 25.60±2.66（P<0.01）. Conclusion Platelet -activation exists in asthmatic mice and may participate in the asthmatic process.
    Effects of mycophenolate mofetil on neural function of experimental autoimmune encephalomyelitis in rats
    YOU Wei,BI Jian-zhong,XIE Zhao-hong,ZHU Zheng-yu,WANG Xiao-yun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  574-578. 
    Abstract ( 1070 )   PDF (566KB) ( 388 )   Save
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    To explore the effects of mycophenolate mofetil on the improvement of neural function and brain and spine cord of rats with experimental autoimmune encephalomyelitis (EAE). MethodsWistar rats were induced by guinea pig spinal cord homogenate and complete freund adjuvant to establish EAE models, while the control rats were induced by saline and complete freund adjuvant. There were 7 control rats and 25 EAE rats which were successfully made and survived. The EAE rats were randomly divided into three groups: the high dose of mycophenolate mofetil treatment group（n=8）, the low dose of mycophenolate mofetil treatment group（n=8）, and the model group（n=9）. Medicine was administered for 14 days and then the Duckers score and weight changes were assessed every day. After 14 days, pathological changes of brain and spine cord were observed by Hematoxylin and Eosin staining. ResultsThe achievement ratio of the EAE model was 72.1%. Compared with the control group, the Duckers score of the EAE rats increased with a decrease of weight(P＜0.01), pathological changes manifested inflammatory cells infiltration around vessels with HE staining. Compared with the model group, after treatment with mycophenolate mofetil, the Duckers score of the EAE rats decreased with an increase of weight and pathological changes lessened, moreover， the therapeutic effect of high dose mycophenolate mofetil was superior to low dose mycophenolate mofetil(P＜0.01). ConclusionMycophenolate mofetil can improve the symptoms of EAE rats and promotes the recovery of neural function in a dosedependent manner.
    Effect of VLDL on the expression of the human urate transporter
    LI Cheng-qian,ZHAO Jia-jun,WANG Xia,LI Chang-gui,MIAO Zhi-min
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  579-581. 
    Abstract ( 1236 )   PDF (855KB) ( 517 )   Save
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    To observe the effect of VLDL in different concentrations on the expression of the human urate transporter (hOAT1) gene in HK-2 cells. Methods HK-2 cells were divided into the control (cultured with DMEM), the V1 (cultured with DMEM and 50umol/L VLDL) and the V2 (cultured with DMEM and 400?umol/L VLDL) groups. In each group, 6 bottles of cells were cultured in vitro for 48 hours, and then the relative quantity of hOAT1 mRNA in the HK-2 cells was determined by real-time fluorescent quantitative PCR assay. ResultshOAT1 mRNA could be detected in all samples, and it was significantly higher in the control group than in the other groups, and in the V1 group it was 41.6% of that in the control group and in the V2 group it was only 34.5% of that in the control group. ConclusionsVLDL can downregulate the expression of the hOAT1gene possibly induced by lipodystrophy easily acting with hyperuricemia.
    Effects of substance P and its receptor in hepatic immune injury induced by Concanavalin A in mice
    ZHANG Hai-tao,YAN Ming,SHANG Nan,LIU Hui-min,ZHANG Yu-quan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  582-585. 
    Abstract ( 1071 )   PDF (521KB) ( 434 )   Save
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    Objective To investigate the role of substance P and neurogenic inflammation mediated by SP in hepatic immune injury induced by concanavalin A(Con A). MethodsHepatic immune injury in mice was established by injection with 20mg/kg Con A through the tail vein. A saline control group was established by injection with saline. Liver damage was assessed by histological evaluation; SP contents in liver homogenates of the two groups were determined by enzymelinked immunosorbent assay (ELISA), and neurokinin-1 receptor expression was determined by reverse transcription and PCR(RT-PCR). ResultsHepatic immune injury in mice was successfully established, and hepatocellular apoptosis and granulocyte infiltration were found in livers in the model group. SP contents in liver homogenates were significantly increased in the model group（P＜0.01）. Moreover, NK-1R mRNA expression in the model group was obviously higher than that in the control group （P＜0.05）. ConclusionSP and its receptor participate in the process of inflammatory liver injury induced by Con A and play an important role in induction and maintenance of inflammation.
    Effect of rrVEGF165 on serum endostatin, BDNF and its TrKB receptor after spinal cord ischemia-reperfusion injury
    JIANG Li,YU Ling-zhi,LIU Xu-dong,GUO Lan-min
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  586-589. 
    Abstract ( 1187 )   PDF (334KB) ( 330 )   Save
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    Objective To investigate the effect of recombinant rat vascular endothelial growth factor 165 (rrVEGF165) on serum endostatin variations and brain derived neurotrophic factor (BDNF) with its tyrosin-kinase B（TrkB）receptor mRNA expression in spinal cord tissues. Methods Forty-eight rats were randomly divided into three groups: the sham-operation group(S), the model group(M) and the treatment group of rrVEGF165(V). The spinal cord ischemia-reperfusion lesion models were established by using Naslund abdominal aorta occlusion and declamped 90 minutes later. Histopathological changes in the spinal cord were observed by hematoxylin and eosion (HE) staining. Serum endostatin and BDNF levels were determined by enzyme linked immunosorbent assay (ELISA). Expressions of TrkB receptor mRNA were determined by reversetransferent polymerase chain reaction (RTPCR). ResultsInconvertible spinal damage was model group, histopathological changes in the spinal cord of group V were remarkably improved. For serum endostatin(ES): in group M, the level of endostatin began to increase after 6h post-reperfusion and remained till day 7. In group V, ES was increased at 6?h after reperfusion but that was not maintained in the following days. Serum BDNF and expression of TrkB mRNA: in group M, serum BDNF was decreased at 6?h, then returned to the control level at day 2, but was decreased more severely at day 7 which was only about 59.1% of the control level and was significant lower than that at 6h(P＜0.05). The expression of mRNA of spinal cord TrkB receptor was up-regulated at 6?h, returned to the control level at day 2, and then kept down-regulating to the lowest level at day 7 after reperfusion. In group V, in contrast, serum BDNF was significantly increased at 6?h, then returned back to the control group at day 7, and the expression of TrkB mRNA maintained a sustainable upregulation level. ConclusionSpinal rrVEGF165 administration can alleviate the increase of ES and the decrease of serum BDNF induced by spinal cord ischemic reperfusion injury, and enhances the up-regulation of mRNA expression of TrkB receptor.
    Construction of a plasmid expressing siRNA aimed at the H-2Kd gene and expression of H-2Kd in mouse LAK cells
    ZHUANG Xue-wei,XIA xi-yan,SHAN Ning-ning,WANG Hong-chun,ZHANG Yi,YANG Xiao-jing,LI Xiao-li,ZHAO Sheng-mei,ZOU Xiong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  590-593. 
    Abstract ( 1096 )   PDF (556KB) ( 386 )   Save
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    To construct the siRNAs expressing plasmid aiming at the H-2Kd gene and to explore the H-2Kd expression in mouse LAK cells and function of H-2Kd with the RNA interference technology. MethodsFirst, a target sequence of the H-2Kd gene was selected, then according to the sequence, two complementary oligonucleotides were lisated into psilencer 3.0-H1, which was transformed into DH5a bacteria, and then it was amplified and purified to get the plasmid. The purified plasmid was identified by gel electrophoresis and sequencing. H-2Kd-targeting siRNA plasmid was used in spleen lymphocytes of BALB/C mice. Flow cytometry(FCM) was used to determine the expression of H-2Kd on the transfected and control cells. ResultsGel electrophoresis and sequencing results showed that the plasmid was about 2.8kb which was identical with the positive control, and the sequence was identical with what had been inserted. Also siRNA treatment significantly inhibited the expression of the targeted protein. Conclusion siRNAs expression plasmids aiming at the H-2Kd gene have been successfully constructed and could inhibit the H-2Kd expression.
    CABG with off-pump technique or cardiopulmonary bypass for serum Endothelin-1 and left ventricular function
    ZHANG Meng-yuan,XU Yan-bing,YAN Qing-fu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  594-596. 
    Abstract ( 1013 )   PDF (329KB) ( 385 )   Save
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    To explore the effects of coronary artery bypass graft(CABG) surgery performed by using both standard cardiopulmonary bypass (CPB) or the octopus technique on Endothelin-1(ET-1) levels and left ventricular function. MethodsSixty-two patients undergoing CABG surgeries were randomly divided into two groups: the G1 group (n=30) which received CPB-CABG and the G2 group(n=32) which received off-pump CABG (OPCABG). Blood samples were obtained before anesthesia (T1), before operation (T2), 6h(T3) and 24h(T4) after the end of operation. Serum Endothelin-1, CI and LVSWI were determined at each time-point. ResultsSerum ET-1 of the G1 group was significantly increased at T3 and T4 time-points and of the G2 group was decreased at T2 timepoint and increased at T4 timepoint. Serum ET-1 of the G2 group was decreased at T3 and T4 time-points compared with the G1 group (P<0.05). Compared with T1 time-point, CI of the G1 group at T2 and T3 time-points and that of the G2 group at T2 time-point were markedly decreased (P<0.05), and also LVSWI of the G1 group at the T3 and T4 timepoints were markedly decreased (P<0.05). Compared with the G1 group, CI and LVSWI of the G2 group at the T3 time-point were increased (P<0.05). Conclusion Off-pump CABG can decrease the output of ET-1 and has a protective effect on myocardial injury and it is superior to CPB-CABG.
    Effects of ketamine and midazolam on heart rate variability in children
    WANG Shu-qin,MENG Qing-li,LEI Wei-fu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  597-599. 
    Abstract ( 1064 )   PDF (259KB) ( 321 )   Save
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    To study the effects of ketamine and midazolam on heart rate variability (HRV) in children. Methods Forty-four children aged 5 to 6 years old (ASA I) were randomly divided into two groups, and intravenously received 2mg/kg ketamine (n=23) or 0.2mg/kg midazolam (n=21). All patients kept spontaneous respiration. Heart rate (HR), mean arterial pressure (MAP) and pulse oxygen saturation (SpO2) were monitored. HRV parameters including total power (TP), low frequency component (LF) and high frequency component (HF) were recorded before and after drug administration. Normalized unit power was derived as follows: nuLF=LF/TP×100%, nuHF = HF/TP×100%. ResultsThe levels of TP and HF decreased (P<0.05), nuLF increased from （63±16）% to （71±13）% (P<0.05) and nuHF attenuated from （32±15）% to （21±13）% (P<0.05) after ketamine administration. While after receiving midazolam, the value TP and LF reduced（P<0.05）, nuLF decreased from（65±15）% to （48±16）%（P<0.05）and nuHF increased from （33±15）% to（ 42±16）%（P<0.05）. ConclusionKetamine and midazolam have different effects on HRV in children, indicating enhancement of sympathetic nerve tension by ketamine and inhibition of sympathetic nerve tension by midazolam.
    Expression of CK19 mRNA and CEA mRNA in peritoneal cavity washes of patients with gastric carcinoma and its clinical significance
    XU De-guo,WANG Wei-bo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  600-603. 
    Abstract ( 1075 )   PDF (282KB) ( 479 )   Save
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    To explore the effect of CK19 mRNA and CEA mRNA expressions in peritoneal cavity washes for gastric cancer. MethodsPeritoneal irrigating solution or abdominal dropsy was collected from 67 patients with advanced gastric cancer and 8 patients with benign lesion on the stomach. Nested RT-PCR and cytopathological examination were used to determine the CK19 mRNA and CEA mRNA expressions. Meanwhile, the gastric cancer cell line SGC7901 served as a positive control. ResultsPositive expression of CK19 mRNA was found in 39 patients (58.2%), of CEA mRNA was in 36 patients(53.7%), and of CK19 mRNA and CEA mRNA was in 45 patients(67.2%) by nested RT-PCR. However, positive expression of both CK19 mRNA and CEA mRNA was found in 13 patients(19.4%) by cytopathological examination. There was a close correlation between CK19 mRNA and CEA-mRNA expressions and pathology and TNM staging in gastric cancer patients. ConclusionsNested RT-PCR is a sensitive method for CK19 mRNA and CEA mRNA expression in gastric cancer, and it is promising as a valuable method for predicting celiac implantation metastasis in gastric cancer.
    Classification and diagnostic prediction of lung cancer using protein profiling of serum and bioinformatics
    HAN Ming-yong,LIU Qi,YU Jie-kai,ZHENG Shu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  604-607. 
    Abstract ( 1132 )   PDF (546KB) ( 212 )   Save
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    To identify serum biomarkers that distinguish lung cancer from healthy individuals by protein fingerprint pattern. Methods86 lung cancer patients and 80 healthy controls were determined by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Results4 protein peaks were selected and used by artificial neural network (ANN) to establish a diagnostic model. The specificity of the diagnostic pattern was 100% and sensitivity of the pattern was 93.6％. ConclusionsA diagnostic model of lung cancer was successfully established, which has high sensitivity and specificity.
    Expressions and clinical significance of ICAM-3 in acute leukemia
    CHEN Ling,YU Gui-lan,LI Yuan-tang,ZHAO Guang-ling,XU Gong-li
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  608-611. 
    Abstract ( 1107 )   PDF (290KB) ( 624 )   Save
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    To explore the expressions of the intercellular adhesion molecule-3（ICAM-3） in acute leukemia and to investigate its role in clinical therapeutic efficacy and extramedullary infiltration (EI). MethodsThe expressions of ICAM-3 in 33 cases of acute nonlymphoblastic leukemia（ANLL）, 21 cases of acute lymphoblastic leukemia (ALL) and 10 normal bone marrow mononuclear cells were determine by bone marrow smear immunohistochemistry. ResultsThe expression of ICAM-3 in AL patients was significantly higher than that in the controls, and it had no correlation with patients′ age, gender, Hb and BPC, but had direct correlations with the ratio of primitive bone marrow cells. Also the expression of ICAM-3 was higher in patients whose WBC≥100×109/L than in patients whose WBC＜100×109/L(P＜0.05), and also was much higher in AL patients without complete remission (CR) than in AL patients with CR(P＜0.01), but there were no significant differences between patients with CR and the controls(P＞0.05). Meanwhile the expression of ICAM-3 was much higher in leukemic cells with EI than that in leukemia cells without EI(P＜0.01). ConclusionICAM-3 could be used as a helpful marker to assess the therapeutic efficacy and prognosis for AL, and its increase might relate to infiltration of acute leukemic cells.
    Relationship between XRCC3 gene polymorphism and bladder cancer in the Han population
    HAO Gang-yue,ZHANG Yue-ying,ZHANG Wei-dong,YANG Ming-shan,JIA Qing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  612-615. 
    Abstract ( 1250 )   PDF (342KB) ( 486 )   Save
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    To investigate the relationship between polymorphisms of DNA repair gene XRCC3 and the risk and clinical outcome of bladder cancer. Methods In a hospital based case-control study for the Chinese population, a total of 307 bladder cancer cases and 316 age- and sex-matched controls with urological non-neoplastic diseases were collected. We analyzed the genotypes of XRCC3 (Thr241Met) by PCR-RFLP and determined their association with bladder cancer risk and clinical outcome. ResultsThe frequency of Thr/Thr, Thr/Met and Met/Met genotype was 87.3%, 12.1% and 0. 6% in cases and was 92.4%, 7.3% and 0.3% in controls. When Thr/Thr served as the reference group, variant genotypes (Thr/Met and Met/Met) had a significant increased risk of bladder cancer（OR, 1.77；95%CI, 1.04-3.02）. Combined with smoking and clinical types, no significant interaction could be found. However, the variant genotypes were associated with an increase of recurrence risk among superficial bladder cancer(OR，3.08；95%CI，1.13-8.41). Conclusions XRCC3 gene polymorphism may be a risk factor for bladder cancer and it may serve as a molecular marker for monitoring bladder cancer recurrence.
    Distribution of microlymphatic vessels in lung adenocarcinoma and its clinical significance
    CONG Bo,PENG Chuan-liang,DONG Xiao-peng,ZHAO Xiao-gang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  616-619. 
    Abstract ( 1105 )   PDF (589KB) ( 400 )   Save
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    To investigate the relationship among vascular endothelial growth factor-C, (VEGF-C), VEGF-D, microlymphatic vessel density (MLVD) and lymph node metastasis in lung adenocarcinoma, and to explore the mechanism of cancerous peripheral tissue lymphatic vessels. Methods The expressions of VEGF-C and VEGF-D mRNA were determined by the semi-quantitative reverse transcriptase-polymerase chain reaction method and the expressions of VEGF-C, VEGF-D and MLVD were determined in the center of the tumors and peripheral tissues by means of immunohistochemistry in 48 cases of lung adenocarcinoma. ResultsExpressions of VEGF-C and VEGF-D mRNA were higher in lung cancer than in normal lung tissues, positive ratio of VEGF-C and VEGF-D was 70.8%(34/48) and 58.3(28/48). The expressions of VEGF-C and VEGF-D in cancerous peripheral tissues were significantly higher than those in the center of the tumors. The MLVD and positive lymph node metastasis in the positive VEGF-C or VEGF-D group were higher than those in the negative group. Conclusion The expression of VEGF-C and VEGF-D can induce lymphangiogenesis of lung adenocarcinoma and lymph metastasis.
    Association between two concurrent gene polymorphisms and type 2 diabetes with macro-angiopathy
    ZOU Cai-yan,LI Yin-yin,LI Qiu,CHEN Han-yan,WANG Lai-cheng,LV Xiao-xia
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  620-623. 
    Abstract ( 1098 )   PDF (445KB) ( 418 )   Save
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    To investigate the correlation between polymorphisms of both +299G/A at resistin gene and Pro12Ala at PPARγ2 gene and diabetic macro-angiopathy. MethodGenotypes and alleles of polymorphisms of both genes were determined with polymerase chain reactionrestriction fragment length polymorphism assay (PCRRFLP) of 296 subjects in Han Chinese. Results Both resistin gene +299G/A alleles frequencies in group A (the type 2 diabetes group) and alleles frequencies and genotypes in group B(the type 2 diabetes combined with the macroangiopathy group) were statistically significant from those in the control group(P＜0.05), but P/A alleles frequencies and genotypes to of the PPARγ2 gene were statistically different in groups A (P＞0.05 vs the control group) and were statistically different in group B (P＜0.05 vs the control and the A group). Type 2 diabetes with the coexistence of the A allele/AA genotype of resistin gene and the A allele/PA genotype of the PPARγ2 gene had a lower incidence of macro-angiopathy than those with one gene alone or without the gene(P＜0.05). ConclusionThe A allele/AA genotype at resistin gene of +299 and the Ala allele at the PPARγ2 gene of Pro12Ala are related to diabetic macroangiopathy.
    Pentacam and IOLMaster in keratometry and anterior chamber depth measurements: a contrast study
    PAN Hong,WANG Li-hua,MA Lu-xin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  624-627. 
    Abstract ( 1194 )   PDF (358KB) ( 325 )   Save
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    To explore the differences of keratometry and antesior chamber depth (ACD) determined by Pentacam and IOLMaster and to evaluate the agreement of the two instruments. Methods169 left eyes of 169 subjects aged （20.80±1.29） with spherical Equivalent-（-3.44±2.04）D were determined keratometry and ACD on both eyes with Pentacam and IOLMaster. Paired t test was used to analyze the difference of mean keratometry (K) ［K=（K1+K2）/2］ and ACD between the two methods, and the BlandAltman method was used to evaluate the agreement of the two methods. ResultsThe mean K value was（43.35±1.49）D for Pentacam and （43.84±1.54）D for IOLMaster (P＜0.01), and the ACD value was（3.65±0.21）mm for Pentacam and（3.70±0.22）mm for IOLMaster（P＜0.01). The BlandAltmann showed that the two modalities had incomparable results for K values and comparable results for ACD. ConclusionPentacam and IOLMaster cannot be interchanged for keratometry, but can be interchanged for ACD.
    Modified ultra-filtration on recovery of infants after open heart surgery
    SHI Guang-yong,ZHANG Gong,LI Yue-hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  628-630. 
    Abstract ( 1031 )   PDF (261KB) ( 314 )   Save
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    To investigate the effects of modified ultrafiltration on cytokines after open heart surgeries in infants. MethodsA total of 22 patients with congenital heart diseases were randomly divided into the modified ultrafiltration(MUF)group and the conventional ultrafiltration(CUF) group. Blood specimens taken from the radial artery were determined for HCT, PLT, COP, BUN and Cr levels at the end of surgery. Also serum levels of TNF-a, IL-6, 8 and C3, C4 were determined at 0, 3 and 24?h after the operations. And the urine volume 2h after operations was recorded. ResultsThe HCT, PLT and COP levels were significantly different between the two groups(P＜0.05), and the TNF-a and IL-6, 8 levels were significantly lower in the MUF group than in the CUF group(P＜0.05), but the C3 and C4 levels were not significantly different between the two groups（P＞0.05）. ConclusionModified ultrafiltration could decrease the inflammatory response and the kidney′s water load after open heart surgeries in infants.
    Orthodontic technique and rigid internal fixation with titanium microplates
    in treatment of maxillo-facial bone stale fractures
    ZOU Ya-nan,ZHANG Jun,LIN Cong,REN Xu-sheng,WANG Xu-xia
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  631-633. 
    Abstract ( 1153 )   PDF (339KB) ( 316 )   Save
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    To explore the effect of rigid internal fixation of titanium microplates allied with the orthodontic technique against maxillo-facial bone stale fractures. MethodsOrthodontic technique and rigid internal fixation with titanium microplate were used in 45 patients suffering from maxillo-facial bone stale fractures. 3D spiral CT was used to define the position and extent of fractures. Rigid internal fixation of titanium microplates was used in fixation of fractures. Orthodontic treatments were proved after surgeries. ResultsAfter a follow-up for 4-18 months, all patients regained bilateral symmetrical faces, normal mouth opening and occlusion recovery. ConclusionRigid internal fixation of titanium micro-plates allied with orthodontic technique is a good choice for maxillofacial bone stale fracture treatment.
    Semi-laminectomy spinal canal reconstruction in treatment of burst thoracolumbar fractures
    ZOU Yun-tao,CHEN Wei-ge,GAO Xue-feng,GONG Wei-bin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  634-636. 
    Abstract ( 970 )   PDF (633KB) ( 421 )   Save
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    To explore a new operative method for thoracolumbar burst fractures(TLBF). MethodsFortyseven patients with TLBF received posterior improved semilaminectomy spinal canal decompression and GSS internal fixation. Results All were followed up for an average of 32.6 months. The preoperative average Cobb's angle was 30°, and the post-operative average angle was 9°. Based on Frankel′s grading, nervous function improved by grade one to three in 38 patients. ConclusionThis method has advantages of less injury, less bleeding, simple manipulation, thorough decompression and reliable fixation. It can return the volume of the spinal canal to normal, reconstruct spinal stability and improve spinal cord or nerve function.
    Echocardiography and BNP in assessment of the cardiacfunction in acute myocardial infarction
    LIU Jun,ZHANG Rong-cheng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  637-639. 
    Abstract ( 1178 )   PDF (232KB) ( 321 )   Save
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    To discuss the alteration of echocardiography and serum Btype natriuretic peptide(BNP) level in patients with ST-elevated myocardial infarction(STEMI) after facilitated percutaneous coronary intervention (PCI). Methods 27 cases were first attacked by STEMI in 12 hours and divided into two groups: 10 cases were treated with primary PCI and 17 with facilitated PCI. Results Compared with primary PCI groups, facilitated PCI groups had good cardiac function as detected by echocardiography at 1 month after PCI, while they had decreased serum BNP levels at 3 days and 1 month after PCI. Conclusion Facilitated PCI can effectively and safely protect cardiac function of patients with STEMI.
    Inhibitory effects of lead-excretion glycan on genetoxic and reproduction toxicity induced by lead in mice
    ZHAO Hui-juan,LIU Ping,ZHUO Jin-hua,FENG Guo-chang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(6):  640-643. 
    Abstract ( 1015 )   PDF (271KB) ( 279 )   Save
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    To study the influence of lead-excretion glycan on micronuclei formation in bone marrow and peripheral blood polychromatic erythrocyte (PCE) and on sperm abnormalities induced by lead in mice. Methods The KM strain mice were randomly divided into the negative control group, the positive control group, the lead control group, the high dosage group, the middle dosage group, the low dosage group and the preventative group based on body weight. The mice were given lead acetate by intraperitoneal injection and treated with lead-excretion glycan by intragastric administration. Micronucleus tests and sperm aberration tests were used． Results The micronuclei formation in bone marrow and peripheral blood PCE of the high and middle dosage groups were significantly less than that of the lead control group（P＜0.05）. The sperm abnormality rate of the high and middle dosage groups and the preventative group was significantly lower than that of the lead control group（P＜0.001）. Conclusion Leadexcretion glycan can to some extent inhibit the increase of micronuclei formation in bone marrow and peripheral blood PCE and sperm abnormalities in leadexposed mice.
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