Table of Content

  

24 May 2006
Volume 44 Issue 5

Articles


Articles

Proteomic analysis of multiple sclerosis cerebrospinal fluid

ZHANG Xuhua,LIU Shilian,QIN Yanjiang,JING Yongsheng,XU Guangrun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(5):  438-442. 

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Objective: To explore the role of some special cerebrospinal fluid proteins on the pathogenesis of multiple sclerosis. Methods: Twodimensional gel electrophoresis and peptide mass fingerprinting were used to identify the proteins in cerebrospinal fluid (CSF) from patients with multiple sclerosis (MS) and patients with nonMS inflammatory central nervous system (CNS) disorders. Results: Different twodimentional maps were received because of the above differences. A total of 35 protein spots were identified that were not seen on the control gel. Most of the proteins were known to be present in normal human CSF, and 6 of them had been identified as MS special by others, but only one protein presented in this study. Conclusion: Four proteins have been identified in multiple sclerosis, and their relation should be further studied.

tromal fibroblasts downregulates the transcription and expression ofHLAA and CD86 inoral squamous cell carcinoma

WANG Dong-guan,SUN Shan-zhen,SHI Lin,WANG Zhen-guang,WANG Xia

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(5):  451-454. 

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Objective:To study the role of tumorstromal fibroblasts(TSF) on the transcription and expression of human leukocyte antigen A (HLAA) and CD86. Methods: Tca8113 cells (oral tongue squamous carcinoma cell line) were cocultivated with TSF in a noncontact manner by Millicell chamber, and then the transcription and the expression of HLAA, HLA B, HLAG and CD86 of Tca8113 cells were detected by semiquantitive RTPCR, Western blotting and flow cytometry analysis. Results: The transcripts of HLAA and CD86 of Tca8113 were downregulated by TSF to 66.49% and 52.31%, respectively compared with the control (P<0.05); But HLAB and HLAG were not influenced almost. Conclusion: TSF downregulates the expression of HLAA and CD86 in oral squamous cells, and therefore the escape from immune may result in tumor progression and metastasis in vivo.

Role of saponins on MCP1 and ET in serum and cerebrospinalfluid of patients with acute brain infarction

WANG Pin,YU Jun, ZHOU Qing-bo,XIE Zhao-hong,BI Jian-zhong,WANG Xiao-yun,LIU Zhen,SUN Lin,WANG Ping

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(5):  455-458. 

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To investigate the concention changes of the monocyte chemoattractant protein1(MCP1) and the endothelia(ET) in serum and cerebrospinal fluid(csf) of patients with acute brain infarction treated by saponins. Methods: The level of MCP1and ET in different times in serum and cerebrospinal were determined by enzymelinked immunoadsordent (ELLISA) and radioreceptor assay (RRA). CSF samples were obtained on the first day of the onset and at 48 hours later during the lumbar puncture procedure. Results: Both the serum levels of MCP1 and ET in different times were notably higher in ischemic stroke than healthy man(P<0.05). The level of MCP1 reached the peak on the day 3 and then decreased on the day 510, and that of ET, too.The levels of MCP

Controlling the depth of propofol anesthesia using the bispectral indexand the systolic blood pressure

WANG Huan-liang,SUN Bao-zhu,DU Hong-mei,ZHOU Chang-qing,ZHANG Li

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(5):  471-474. 

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To compare the effect of the bispectral index (BIS) and the systolic blood pressure (SBP) on controlling the depth of propofol anesthesia. Methods: BIS (the B group) or SBP (the S group) at random was used to titrate the propofol anesthesia in 40 patients scheduled for hysterectomy. The consistency of the value of BIS and SBP to the set control value, the consumption of propofol and the emergence time were compared. Results: About (89 ±10) % of the measured BIS time points in group B, and (49±29) % in group S were maintained in the target field (50±10), (P<0.001). Significantly higher incidence of BIS levels < 40 was recorded in the group S than in the group B ［(44±31)% vs (9±10)%］ and that of BIS> 60 was recorded in the group S than in the group B［(7±16) % vs (2±2) %］ (P<0.01). Although SBP was the controlled variable in the group S, adequate hemodynamic stability (within the 15mmHg range around baseline) occurred more frequently in the group B (51±27) % in comparison with group S (34±31)%, (P< 0.05). The incidence of too low SBP, ［(41±33)% in the group B vs (64±31)% in the group S］ and the incidence of too high SBP, ［(7±11)% in the group B vs (1±2)% in the group S］ were both significantly different between the two groups (P<0.05). Recovery was quickd the consumptions of propofol were lower in the group B than those in the group S (P<0.05). Conclusion: As a controlled variable of anesthesia, BIS has more advantages in keeping the stability of anesthesia and hemodynamics than SBP.

Docetaxel sensitizes human lung adenocarcinoma cellsto TRAILinduced apoptosis

ZHOU Wei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(5):  531-535. 

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microscope and by MTT assay, respectively. Annexin ⅤFITC and Propidium iodide(PI) were used to stain A549 cells, and flow cytometry(FCM) was used to determine the apoptosis after the treatment of TRAIL or/and TXT. As well as reverse transcriptionpolymerase chain reaction (RTPCR) was applied to semiquantitatively assay the mRNA expression of death receptors DR4 and DR5 in A549 cells before and after the treatment of subtoxic doses of TXT for different hours. Results: ① The growth inhibition by TXT worked in a dosedependent fashion on A549 cells. The value of IC50 of TXT was 62.1ng/ml. The growth inhibition rate (GIR) was (6.84±1.14)%, and (26.10±4.00)% respectively by TRAIL at the concentration of 100?ng/ml and 1?600?ng/ml and in an acting time of 24 hours, and it was (19.98±4.15)% by TRAIL at 100?ng/ml in the presence of TXT 4?ng/ml. There was significant difference in the growth inhibition rate (GIR) by TRAIL before and after the combination of TXT(P＜0.05); ② The apoptotsis rate detected by FCM was 4.44%, and 12.26% respectively after the 24 hours treatment by TRAIL(100?ng/ml) or TXT(4?ng/ml), whereas it was increased to 16.84% after the 24 hours treatment of TRAIL(100?ng/ml) combined with TXT(4?ng/ml); ③ The expression level of DR4 and DR5 mRNA in A549 cells had no significant change after the treatment by TXT(4?ng/ml) for 4 hours and 8 hours (P>0.05). Conclusions: Human lung adenocarcinoma A549 cells are not sensitive to TRAIL, but are sensitive to docetaxel on TRAILinduced apoptosis. The effect of TXT reversal on TRAIL apoptosis pathway is not supposed to be related to the upregulation of death receptors DR4 and DR5 mRNA in A549 cells.