Table of Content

  

24 August 2006
Volume 44 Issue 8

Articles


Articles

Stress in different intensity and duration effects the habitual expression of HSP70mRNA on the hippocampus of rats

SHAO Shu-hong,LI Zun-ling,JIANG Hong,WU Jiang,PAN Fang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(8):  770-773. 

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To study the habitual expression of HSP70mRNA in hippocampus of rats by forcedswimming stress, and therefore to explore the effect of different intensity stress on the HSP70mRNA expression. Method: RTPCR was used to observe the changes of HSP70mRNA expression in hippocampus. Result: Repeated forced swimming in warm water resulted in expressive habituation, and an additive forced in 4?℃ water led to higher HSP70mRNA expression. Conclusion: Repeated stress may induce the habituation of the HSP70mRNA expression, and intense stress may reverse the habituation induced by repeated chronic stress.

Multinomial logistic regression for affecting factors of cervical erosion

CHEN Hui-bo,LIU Hong-qing,LI Hui-qing,ZHENG Wei,ZHENG Guo-hua,HUANG Ke-feng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(8):  778-780. 

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o explore the affecting factors in cervical erosion. Methods: Cluster randomized sampling method for selecting subjects and multinomial logistic regression were used to analyze these data． Results: The prevalence of cervicalerosion Ⅰ，Ⅱ and Ⅲ degree was 12.47%，7.95%，3.70% respectively, and the affecting factors included age，times of bathe in a whole year, times of artificial abortion, hygiene situation before sexual intercourse, and infection of ureaplasma urealyticum. Conclusion: In order to prevent cervical erosion effectively， artificial abortion should be avoided as possible as they can, good sanitary habits should be built up and infection in reproductive tract should be detected and treated as early as possible too.

arlic oil can prevent the decrease of leucocyte induced by radiation in mice

ZHANG Cui-li,XIE Ke-qin,ZHAO Xiu-lan,ZHU Zhen-ping,YU Li-ua,ZENG Tao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(8):  781-784. 

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To explore the effect and mechanism of garlic oil in preventing the decrease of white blood cell (WBC) induced by radiation in mice. Methods: BALB/c male mice were distributed to 6 groups according to WBC amount: normal, radiative model, positive Li2CO3, garlic oil low, middle and high dose groups. The changes of the peripheral WBC level, the spleen's coefficient, the amount of spleen nodes and the content of DNA in marrow were measured. Results: Compared with the radiative model group, the amount of peripheral WBC of garlic oil low, middle and high dose groups was increased by 51%, 59% and 350%, respectively; The content of DNA in marrow of garlic oil middle and high dose groups was enhanced by 56% and 82%, respectively; The spleen′s coefficient of garlic oil in middle and high dose groups was advanced by 20% and 39%; The amount of spleen nodes of garlic oil in low, middle and high dose groups were increased by 41%, 65% and 71% (P＜0.05). Compared with Li2CO3 positive group, the amount of peripheral WBC of garlic oil in high dose group was increased by 117%; The spleen′s coefficient of garlic oil in high dose group was advanced by 17% (P＜0.05). Conclusion: Garlic oil can prevent the WBC decrease induced by radiation at some extent possibly by stimulating the marrow hyperplasia, the spleen and the spleen nodes hyperplasia.

Cause of the difference on inhospital mortality between male and female patients with acute myocardial infarction

JIANG Shi-liang,JI Xiao-ping,WANG Xiao-rong,SONG Zhao-feng,ZHANG Yun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(8):  785-788. 

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December 2004. Variables including baseline characteristics, inhospital management and outcomes were recorded and compared between different gender groups. Results: Females were generally older than males (67.7±8.5 vs 60.4±11.6 years, P＜0.001) and had a higher prevalence of hypertension（51.40% vs 39.25%, P＜0.001）and diabetes mellitus（28.31% vs 14.69%, P＜0.001).The Killip class greater than or equal to Ⅲ（11.92% vs 5.22%，P＜0.001） and the total cholesterol (TC)＞4.68?mmol/L（73.37% vs 57.22%，P＜0.001）were more common in females. There was no significant difference on the history of previous angina pectoris, myocardial infarction and family coronary heart disease between males and females (64.53% vs 66.85%，P＝0.344；8.91% vs 9.87%，P＝0.519；22.98% vs 20.68%，P＝0.348, respectively）, however, the history of cigarette smoking（69.74% vs 14.71%, P＜0.001) and alcohol drinking（78.49% vs 24.77%，P＜0.001) was more common in males. Reperfusion therapy within the first 24 hours after symptom onset and β－blockers were underused during hospitalization phase in females compared with males (22.16% vs 28.01%, P＝0.010; 64.43% vs 70.14%, P＝0.017, respectively). An increased mortality was demonstrated in females during the hospitalization phase of AMI（11.92% vs 6.90%, P＜0.001). Conclusions: The inhospital mortality of females is higher than that of males following AMI. Females are older and have more risk factors than males, and reperfusion therapy and βblockers are underused, all of which are important causes of the difference on inhospital mortality between male and female patients with AMI.

Antitumor immunity of mouse B71 and B72 vaccine: a comparative study

YAN Shu-xin,QIN Xue-mei,YU Ling,MA Dao-xin,XU Cong-gao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(8):  789-793. 

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To assess and compare the potenti of mouse immunecostimulating signal B71 and B72 vaccine in inducing antitumor immunity effect. Methods: Reverse transcription expressing vector pLXSNmB71 and pLXSNmB72 were constructed to transfect wild EL4 cells, then mB71 gene and mB72 gene were introduced into EL4 cells, the positive clone (EL4/mB71 and EL4/mB72) highly expressing mB71 and mB72 molecular was selected. In vitro, The secretion of IL2 of EL4/mB71 and EL4/mB72 vaccine stimulatedlymphocytes, and the T cell functions of proliferation and cytotoxicity were detected by the method of LDHrelease assay, MTT assay and ELISA. Results: ① IL2 production stimulated by EL4/mB71 and EL4/mB72 vaccine was much higher than wild EL4 cells（P＜0.01）; ② IL2 production stimulated by EL4/mB71 vaccine was higher than EL4B72 vaccineat 24 hours (P＜0.05), whereas IL2 production stimulated by EL4/mB72 vaccine was higher than EL4/mB71 vaccine at 48 hours (P＜0.05); ③ In vitro, both mB71 vaccineactivated CTL and mB72 vaccineactivated CTL exhibited a specific cytotoxicity. These two kinds of activated CTL had no significant difference in killing tumor cells (P＞0.05). Conclusion: Both mB71 and mB72 can activate T cell to produce IL2, which get to the peak not at the same time. mB71 and mB72activated CTL exhibit the similar specific cytotoxicity.

Myocardioprotective effect of adenosine preconditioning on open heart surgery

LI Yue-ua,GUO Feng,GONG Xing-un,PANG Xin-an

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(8):  798-801. 

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To explore the myocardioprotective effect and mechanism of adenosine preconditioning in patients undergoing open heart surgery. Methods：Twentythree patients with simple congenital heart defects were randomly divided into two groups：adenosine preconditioning group (adenosine group，n=12) and control group (n=11). The hearts suspended with cool improved St.Thomas cardioplegia (20?ml·kg-1) which was perfused every thirty minutes after aorticclamping in both groups. Adenosine was administered intravenously to the patients slowly in adenosine group at a dose of 2?mg·kg-1 before beginning of cardiopulmonary bypass(CPB). The serum concentration of creatine kinase(CK), creatine kinase MB isoenzyme (CKMB) and lactic dehydrogenase(LDH) from venous blood were measure at different time points after operation. Myocardial tissues were taken from right atrial appendage at the end of the myocardial ischemia time，and the cytohistomorphological changes were observed. Results: After heartreperfusion，the proportion of spontaneous returned heartbeat was 91.6% in adenosine group，but 54.5% in control group (P<0.05). The levels of serum CK, CKMB and LDH in adenosine group were much less than those in control group at different time points(P<0.05 or P<0.01).　The myocardial histomorphological observation showed very metamorphic changes in control group, but slighter changes in adenosine group. Conclusion: Adenosine preconditioning can reduce the release of myocardial enzyme and the ischemic injury of myocardium, which may be one of the mechanisms of adenosine preconditioning.reperfusion，the proportion of spontaneous returned heartbeat was 91.6% in adenosine group，but 54.5% in control group (P<0.05). The levels of serum CK, CKMB and LDH in adenosine group were much less than those in control group at different time points(P<0.05 or P<0.01).　The myocardial histomorphological observation showed very metamorphic changes in control group, but slighter changes in adenosine group. Conclusion: Adenosine preconditioning can reduce the release of myocardial enzyme and the ischemic injury of myocardium, which may be one of the mechanisms of adenosine preconditioning.

Exression of RAGE in diabetic rat kidney tissue: relationbetween RAGE and podocyte injury

ZHANG Li-ing,JIANG Ling,HOU Zhi-iang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(8):  802-805. 

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To evaluate the changes of RAGE(receptor for advanced glycation endproducts) expression and podocytes in renal tissues of diabetic and control rats, and explore the relation between RAGE and podocyte injuries. Methods: Adult male Wistar rats were injected intravenously with streptozotocin (STZ) to induce diabetes. The rats were sacrificed eight weeks after the induction of diabetes. The renal RAGE and desmin expressions were accessed by semiquantitative immunohistochemistry. The average podocyte number of each glumerulus was determined by dividing the total podocytes by the glomerular profile area as determined by point counting. And correlation analysis was used to evaluate the relationship between RAGE expression and podocytes of each glomerulus. Results: Compare with control rats, the expressions of RAGE and desmin were increased remarkably（P<0.01）, and the podocytes of each glomerulus was decreased significantly（P<0.01） in diabetic rats. There was a negtive relationship between the RAGE expression and the podocytes of each glomerulus（r=-0.736，P<0.01）. Conclusion: RAGE may play an important role in the progression of diabetic nephropathy.

Electrical and geometric changes after percutaneousclosure of atrial septal defect

JIN You-eng,WANG Yu-in,HAN Bo,LIU Ting-iang,ZHANG Jian-un,ZHUANG Jian-in,HAN Xiu-hen,Wang Yi,MA Pei-an

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(8):  806-809. 

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To investigate the electrical and geometric changes of heart after percutaneous closure of atrial septal defect (ASD). Methods: A total of 100 patients received percutaneous closure of ASD, and were measured standard 12lead electrocardiography (ECG) and transthoracic echocardiography(TTE) before closure, 24 hours, 1 month and 3months after closure. The items of ECG included PR interval, QRS duration, PII and RV1+SV5, and that of TTE included the diastolic diameter of right ventricle(RVDD) and left ventricle(LVDD), RVDD/LVDD ratio, the lateral diameter of left atrial (LALD) , the lateral diameter of right atrial (RALD), RALD/ LALD ratio and ejection fraction(EF) of LV. Results: In all patients, the PR interval, RVDD, RALD, RVDD/LVDD and RALD/ LALD ratio decreased and LVDD, LALD, EF increased significantly after closure, but QRS duration, PII and

Effect of fexofenadine hydrochloride sustained capsule for seasonal allergic rhinitis: a doubleblind, randomized, comparative multicenter trial

LEI Da-peng,LI Xue-zhong,XU Feng-lei,PAN Xin-liang,LIN Guo-jing,XUE Wei-guo,LI Chun-yang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(8):  819-822. 

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To study the efficacy and safety of fexofenadine hydrochloride sustained capsule in treating seasonal allergic rhinitis. Methods: A randomized, doubleblind, doubleanalog comparative multicenter trial was performed on 140 patients, who were randomly given either fexofenadine hydrochloride　(n=71) or cetirizine dihydrochloride　(n=69) one tablet in the morning and one in the night.　After a followup on the 7 th day anthe 14 th day, the efficacy and adverse response were monitored at the end of the 2 weeks. Results: Of the 140 patients, 137 or 140 could be evaluated the efficacy or safety, respectively. The total response rate of fexofenadine hydrochloride and cetirizine dihydrochloride was 66.20% and 68.12%, respectively（P＞0.05）. No statistical difference of the adverse reactions was found between the two groups（P＞0.05）. Conclusion: Both fexofenadine hydrochloride and cetirizine dihydrochloride are well tolerated and effective in the treatment of seasonal allergic rhinitis.

Inhibitory effects of recombinant consensus interferon α on different viruses in vitro

XU Bing,WANG Shu-qin,LI Yu-huan,TAO Pei-Zhen

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(8):  831-833. 

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To investigate the antiviral activity of one of the new recombinant consensus interferon α (NIFNcon　α) in vitro. Methods: The cytopathiceffect inhibition assay was applied in this study to investigate the antiviral activity of this drug as well as the other IFNcon α (Infergen) and IFNα as positive control. Vero cells and MDCK cells were infected by different viruses respectively and were cultured with serial dilutions of NIFNcon α. Results: The TD50 of NIFNcon α for MDCK cells and Vero cells was (＞250±0)μg/ml and >192±0?μg/ml， respectively. The IC50 of NIFNcon α for Herpes virus type 1 and type 2 was (2.8×10-4±8.5×10-5) and (1.8×10-4±8.5×10-5) μg/ml, respectively, for coxsackie virus B1 and B3 was (8.8×10-8±1.4×10-8) and (1.5×10-7±4.2×10-8) μg/ml, respectively and for influenza virus A and B was (2.5×10-2±1.2×10-2) and (＞10±0)μg/ml, respectively. Conclusion: The new drug has similar antiviral activities with Infergen, and compare with interferon α, it has more effective antiviral activities for HSV and coxsackie virus except for influenza viruses.

Identification of glyguanid, phenformin, gliclazide and glyburide addedinto traditional Chinese medicine for antidiabetes

LI Bing,WANG Ben-jie,HUANG Ping,WEI Chun-min,GUO Rui-chen

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(8):  843-845. 

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To identify glyguanid, phenformin,gliclazide and glyburide added into traditional Chinese medicine for antidiabetes. Methods: The HPLC and LC/MS methods were established and used to quantitatively identify the components of traditional Chinese medicines, Tangleshu and Ketangmin capsules on the basis of retention time, chemical structure and molecular weight. Results: Phenformin and glyburide were found in Tangleshu capsule, glyguanid and gliclazide were found in Ketangmin capsule. Conclusion: HPLC and LC/MS methods can be used to quantitatively determine the four above mentioned components simultaneously.