Table of Content

  

24 October 2006
Volume 44 Issue 10

Articles


Articles

Differential expression of M2PK in normal cervix and cervical cancer tissues

BAI Xiao-hui,KONG Li,ZHANG Ai-rong,JIA Ji-hui,ZHANG Wei-fang,TANG Wei,YU Xiu-ping

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(10):  981-984. 

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To screen out cervical carcinoma related protein markers by a comparative proteome analysis. Methods: Twodimensional gel electrophoresis maps of the proteins extracted from H8 and Caski were applied respectively. The differential expression proteins were identified by MALDITOFMS; Western blot was used to detect M2PK expression from normal cervix and cervical carcinoma tissues. Results: The 2DE maps of H8 cells and Caski cells were established, and the protein M2PK was identified successfully. Western blot confirmed differential expression of M2PK on normal cervix and cervical carcinoma tissues. Conclusion: These proteins from H8 cells and Caski cells are differentially expressed. It will be helpful to find new molecular markers for early diagnosis and treatment of cervical cancer.

Effect of fibronectin in oral leukoplakia

WANG Ling-li,QI Xiang-min,YAN Shi-guo,ZONG Xiao-ming

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(10):  985-988. 

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To study the expression of fibronectin(FN) in oral leukoplakia(OLK). Methods: Fibroblast and epithelial cells of normal oral mucosa and OLK were cultured in vitro. The level of FN in medium was detected by using ELISA method. The distribution of FN in tissues of normal oral mucosa, OLK and oral squamous cell carcinoma (OSCC) was observed by means of inmmunohistochemistry. The expression of FN in fibroblast and epithelial cells of normal oral mucosa and OLK was observed by using RTPCR technique. Results: The level of FN in medium of OLK fibroblast was lower than that of normal oral mucosa fibroblast markedly by ELISA (P<0.01); the level of FN was not markedly different between epithelium of OLK and normal oral mucosa. The result of RTPCR indicated that the expression of FN was obviously derogated in OLK fibroblast. The result of inmmunohistochemistry showed that the expression of FN in OLK epidermas and connective tissues declined incoordinately compared with normal oral mucosa and the distribution was different in them, but the degree of lesion in OLK was lower than that in OSCC. Conclusion: It is significant that the expression and different distribution of FN in OLK lead to OSCC. This provides a new way for treating OLK and interdicting OLK cancerization.

An experimental study on repairing of facial nerve defects with

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(10):  989-992. 

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To evaluate the effect of complex chitosan conduits containing Schwann cells(SCs) in repairing of facial nerve defects. Methods: Complex chitosan conduits were made from chitosan and carboxymethylchitosan by freeze drying method in proportion of 3 to 1. Schwann cells were obtained from sciatic nerves of new born rabbits, and then were cultured and numberenlarged. After being combined with SCs, the complex chitosan conduits were used to repair a 0.8?cmdefect of facial nerve. The nerve regeneration was evaluated by gross, electrophysiological andstological examinations after 4, 8 and 16 weeks, and were compared with the results of using conduits only. Results: New nerve had bridged the defects 8 weeks after operation, and most of the conduits had degraded 16 weeks after operation. Compared with chitosan conduits only , the conduits+ SCs group had better electrophysiological and histological results. Conclusion: The complex chitosan conduit combined with SCs is a better method for repairing of facial nerve defects.

Expansion of the human bone marrow hematopietic stem /progenitor cells ex vivo

ZHANG Chun-hua,WEN Ze-qing,ZHU Yong,CHEN Xiu-ling

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(10):  993-997. 

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To explore the potential ex vivo expansion ability of the human bone marrow mononuclear cells(MNCs) and AC133＋ selection cells under different cell factor combination stimulation. Methods: This research utilizes the Ficoll to separate human bone marrow MNCs and MiniMACS to gather AC133＋cells. The cell factors were divided into 6 groups. Through the ex vivo comparison cultivation under different cell factor combination stimulation, the expansion and AC133 phenotype of MNCs and AC133＋ selection cells were observed, and the clone formation of the MNCs and AC133＋ selection cells under different cell factor stimulation on different time was observed by using Halfsolid Methyl cellulose. Results: In the same condition, human bone marrow MNCs were expanding much more than AC133＋ cells, and their clone density was more than AC133＋ selection cells（P＜0.05）. In cell factor combination 6 stimulation, human bone marrow MNCs and AC133＋ selection cells were expanding much more than other cell factor combinations （P＜0.05）, and the clone density generated by cell factor combination 6 highered than the clone density generated by other combination stimulations under the same conditions（P＜0.05）. Conclusion: Through the 4week shortterm cultivation, human MNCs are generating more stem cells. Cell factor combination 6 is the first choice for ex vivo expansion.

Histologic and physiological function of cryopreserved ovarian tissues after autologous implantation in female rabbits

XU An-ran,CHAO Lan,ZHEN Jun-hui,YU Hong-ling,DENG Xiao-hui

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(10):  998-1003. 

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To assess the histologic and physiological function of the cryopreserved ovarian tissues after autologous implantation in mesometrium, ovarian bursa and ovary in rabbits. Methods： Fifteen New Zealand White female rabbits were divided into three groups at random, with 5 rabbits in each group. Group 1,fresh ovarian tissues were implanted into mesometrium and ovarian bursa; group 2, cryopreserved ovarian tissues were implanted into mesometrium and ovarian bursa; group 3, cryopreserved ovarian tissues were implanted into ovary. The ovarian and endometrial histology, the survival and development of follicles were detected through light microscope and electron microscope. Vaginal cytology was used to assess the secretary function of implanted ovarian tissues. Results： No significant differences were observed in the proportion of normal and morphologically changed follicles between frozenthawed tissues and fresh ovarian tissues (P>0.05), between cryopreserved tissues and implanted cryopreserved tissues (frozenthawed group compared with group 2 or group3, P>0.05), and among implantation groups (Group 1, 2 and 3, P>0.05, Table 1). TheHJ]percentages of normal follicle in group 1, 2 and 3 were lower than those of the fresh ovarian tissues(P＜0.05). The implanted ovarian tissues in group 1,2,3 all resumed follicle development and no significant differences were observed in the proportion of maturescent follicles between the fresh ovarian tissues and three implanted groups(P＞0.05). The maturescent follicles in tissues of group 2 and 3 were more than those in frozenthawed tissues(P＜0.05). After cryopreservation and implantation, there were no evident changes in ovarian histology and ultrastructure. Conclusion: Cryopreservation of small pieces of ovarian tissues is feasible. After autologous implantation, the cryopreserved ovarian tissues have no significant morphological and ultrastructural changes, the follicles survive and develop well; Mesometrium, ovarian bursa and ovary are all favourable implantation sites.

Expression of MAGE, GAGE and BAGE genes in endometrial carcinoma

WU Xi-mei,ZHANG Shi-qian,ZHOU Xiao-liang,SHI Yan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(10):  1004-1007. 

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To investigate the expression of MAGE and BAGE， GAGE genes in tissues of endometrial carcinoma and normal endometrium，and evaluate the possibility of using these genes as molecular markers and as targets of specific immunotherapy for endometrial carcinoma. Methods：Samples of 35 endometrial carcinomas and 15 normal endometria were examined with reversepolymerase chain reaction（RTPCR） for the expression of MAGE1， MAGE3， BAGE and GAGE12. Results： In 35 endometrial carcinoma tissues， 46％（16/35）， 49％（17/35）, 20％（7/35）and 26％（9/35）of MAGE1， MAGE3， BAGE and GAGE12 mRNAs, respectively were expressed. At least one gene transcription was detected in 77％ of the endometrial carcinoma samples. While no expression was observed in 15 normal endometrial tissues. There was no significant correlation between the expression of MAGE1， MAGE3， BAGE and the clinical stage or histological grade of endometrial carcinoma. There was no significant correlation between GAGE12 and the clinical stage too, but there was a significant correlation between GAGE12 and the histological grade. Conclusions： Our study indicates that MAGE1， MAGE3， BAGE and GAGE12 are relatively high expressed in endometrial carcinoma. These tumorspecific antigens can be used as molecular markers for diagnosis and possible targets for immunotherapy for endometrial carcinoma.

hanges of basic fibroblast growth factor in bladder smooth muscle in patients

YAN Lei,LIU Ben,XU Zhong-hua,ZHOU Zun-lin,CAI Song-liang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(10):  1011-1013. 

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To investigate the changes of basic fibroblast growth factor(bFGF) in human bladder smooth muscle in patients with bladder outlet obstruction(BOO) induced by benign prostatic hyperplasia(BPH). Methods： Sixteen patients with other diseases and forty patients with BPH were included in this study. All patients were divided into three groups, a control group, an obstructive detrusor stability group and an obstructive detrusor instability group. bFGF was measured using an immunohistochemical study. Results： The immunohistochemical study indicated that bFGF was expressed in cytoplasm of bladder smooth muscle cells. The expression of bFGF in the obstructive detrusor stability group and in the obstructive instability group were significantly higher than in the control group (P＜0.05). But there was no significant difference between the obstructive detrusor stability group and the obstructive instability group(P＞0.05). Conclusion： The increased bFGF level of bladder smooth muscle cells is correlated with the alterations of bladder detrusor due to BOO, but may not relate to the detrusor instability.

Brachytherapy by CTguided percutaneous paracentesis implantation of iodine125 seeds for lung cancer

WANG Jing,LI Zhen-jia,WANG Xian-feng,MING De-guo,CHI Xiang-yu

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(10):  1017-1020. 

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To explore the clinical value of 125Methods: Implantation of iodine125 seeds with implanting needle (18gauge) by optical navigating system was carried out in fiftythree patients with lung cancer. After operation， the size of tumors and the changes of blood were observed. Results： The fiftythree patients, with sixtytwo focuses, were given eightyone punctures and observed for more than six months. Twelve of the patients were implanted again. The response rate of one, two, three and six months after operation was 33.87%(21/62), 51.61%(32/62),91.94%(57/62) and 95.16%(59/62) respectively. There were no significant changes in peripheral blood cells, liver functions, renal functions, T cell subgroups and the activity of natural killer cells after operation. Pneumothorax and hemoptysis were seen in 5(6.17%,5/81) and 9 (11.11%,9/81) patients respectively. Conclusion: Brachytherapy by CTguided percutaneous paracentesis implantation of iodine125 seeds is effective and safe for the treatment of lung cancer. The complication is rare and easy to treat.

Differentiation of cardiomyocytes from bone marrow mesenchymal

SONG Zhao-feng,JI Xiao-ping,WANG Xiao-rong,JIANG Shi-liang,ZHANG Xiao-fang,WAGN Rong,ZHANG Yun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(10):  1021-1023. 

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To investigate the factors of differentiation of cardiomyocytes from rat mesenchymal stem cells in vitro． Methods: Mesenchymal stem cells(MSCs) were isolated from cannon born and thighbone of the rats, and were induced by 5azacytidine after culture and expansion. The proliferation and the differentiation were observed. The αactin and the cTnI were detected. Results: αactin and cTnI were found after induced by 5azacytidine. MSCs was similar to cardiomyocytes in physiology. Conclusion: MSCs can be translated to cardiaclike muscle cells after induced by 5azacytidine in vitro.

Antiapoptosis effect of grape seed proanthocyanidin extract

LIANG Ying,GAO Hai-qing,YOU Bei-an,LIU Xiang-ju

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(10):  1036-1039. 

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To study the effect of grape seed proanthocyanidin extract(GSPE) on the apoptosis of myocardial cells induced by myocardial reperfusion injury(MRI) in rabbits. Methods: Twentyfour male New Zealand rabbits were divided into four groups at random: control, model, low dose and high dose groups. After 21 days of treatment, serum CKMB, SOD and MDA of the rabbits of each group were detected at different times before and after reperfusion, the ultrastructure of myocardium and the apoptosis of myocardial cells were observed. Results: The CKMB and MDA decreased, but SOD increased in GSPE group compared with the model group (P＜0.01, P＜0.05). Pathological analysis manifested MRI revived markedly in GSPE group. The amount of apoptotic cells and the apoptosis index (AI) reduced significantly in GSPE group (P＜0.01). Conclusion: GSPE has a significantly preventive effect on myocardial cell apoptosis induced by MRI, except for its potent antioxidant activity ，which may be one of the mechanisms of its anti MRI effect.

Effects of RNAi on HER2/neu gene and proliferation of SKOV3 ovarian cancer cells in vitro

YI Cui-hua,HOU Ming,LI Li-zhen,ZHANG Xin,WEI Jun-min,LI Li,HAO Jing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(10):  1048-1052. 

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To investigate the effects of HER2/neu gene silencing on SKOV3 ovarian cancer cells by RNA interference. Methods： Design and synthesize two siRNAs based on the sequence of HER2/neu mRNA. They were separately subcloned into the plasmid of pGenesil1 containing U6 promoter. The pGenesil1 vectors of the RNA interference eukaryotic expression specific to HER2/neu gene were constructed. The pGenesil1 vectors were transfected into SKOV3 ovarian cancer cells with METAFECTENE in vitro. The positive cell clones were obtained after being screened with 250?μg/ml G418. Then the effects of RNAi on the expression of HER2/neu gene were detected by RTPCR and Western Blot. The cellular growth activities were assayed by CCK8 colorimetry and flow cytometry in vitro. Results： DNA sequencing of the plasmids verified the successful construction of the HER2/neu siRNA vectors. Compared with the blank and the control group, the expressions of HER2/neu mRNA and protein 185 were remarkably downregulated in the RNAi groups; and the cell proliferations decreased in the RNAi groups. Conclusion： We successfully construct the recombinant plasmids of siRNAs specific to HER2/neu gene and transfect them into the SKOV3 ovarian cancer cells in vitro. The RNAi inhibits the expression of HER2/neu mRNA and protein 185, and subsequently suppresses the proliferation of SKOV3 cells.

Effects of BRMS1 gene transfection on invasion potential of breast

TANG Lu-bing,SUN Jing-zhong,GAO Hai-dong,WANG Tian-tian

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(10):  1057-1060. 

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To evaluate the invasion potential of breast cancer cell MDAMB231 transfected by BRMS1 gene in vitro. Methods: A eukaryotic expression vector containing BRMS1 gene was transfected into breast cancer cell MDAMB231 by lipofectamine，and then RTPCR was used to detect the expression of BRMS1mRNA in cells before and after transfection, scanning electron microscope to observe the ultrastructure, and Boyden Chamber to assay the change of invasion. Results: After transfection, BRMS1mRNA was upregulated significantly, the ultrastructure obviously changed, and the ability of invasion significantly dropped off in vitro. Conclusion: BRMS1 gene suppresses the invasion of breast cancer cell MDAMB231 in vitro.

Vesania after kindney transplantation

WANG Hong-wei,LIU Shuang-de,TIAN Chuan,YAN Jie-ke,XU Dong-sheng,ZHENG Wan-hong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(10):  1072-1075. 

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To investigate the causes for vesania in postrenal transplantation patients. Methods： We reviewed and analyzed the process of diagnosis and treatment of 9 patients caught vesania after kidney transplantation. They were all treated using sedatives, 8 of them were transfused using Diazepam, the largest dosage was 90mg within 24 hours.However, 5 of them still showed the symptom of vesania after transfusion, so they were injected with 25?mg Chlorpromazine and 25?mg Promethazine. Results： After being used sedative, the patients were calmed down gradually and then could be treated easily, 5 of the patients were in the state of lethargy with stable vital signs. After 24 to 72 hours, patients were given less sedative till not according to their condition. Being stable, 8 patients were discharged 2872 days later. The transplanted kidney of one sufferer was broken due to anxious. After nephrectomy, he was given Diazepam, and the symptom disappeared 28 hours later. One year later, He accepted transplantation and no symptom of vesania reappeared. Conclusions： Vesania after kidney transplantation contributes to comprehensive causes.

Expression of Maspin and its significance in human nonsmall cell lung cancer

SUN Ping,PAN Xiang-lin,QU Gui-mei,WANG Wei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(10):  1078-1080. 

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To study the expression of Maspin in human nonsmall cell lung cancer. Methods: A immunohistochemical method PictureTM was used to detect maspin in 94 cases of lung cancer, 54 of normal bronchial mucosas and 28 of innocent diseases. Results: Maspin expressed itself in 100.00%， 89.28%, 54.26% of normal bronchial epithelial cell,innocent disease, non small cell lung cancer，respectively（P＜0.05). It correlated with LN(+)and TNM staging but did not with histological type and classification degree. Conclusion: Maspin significantly decreases in NSCLC, and it may be a sensitive predictor of metastasis and prognosis for NSCLC.