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Table of Content

      
    24 April 2007
    Volume 45 Issue 4
    Articles
    Effects of transient transfection of pcDNA3-HBV on dendritic cells
    GAO Li-fen,SUN Wen-sheng,MA Chun-hong,ZHANG Li-ning,LIANG Xiao-hong,WANG Xiao-yan,GUO Chun,CHEN You-hai
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  325-328. 
    Abstract ( 1469 )   PDF (289KB) ( 755 )   Save
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    Objective: To study the role of HBV infection on the function of dendritic cells (DCs) and explore the mechanism of immune tolerance in chronic B hepatitis. Methods: The bone marrow cells were isolated, cultured and transfected transiently with pcDNA3-HBV or pcDNA3 plasmid DNA, and then they were cultured under the stimulation of GM-CSF, IL-4, TNF-α to be induced to become mature dendritic cells. RT-PCR was performed to detect the expression of PreS1 mRNA. FCM was used to detect the expression of CD80, NF-κB. 3H-TdR adulteration assay was used to detect the potential for stimulating the proliferation of allogenic lymphocytes. Results: After being transfected with pcDNA3-HBV, mature DCs had the expression of PreS1 mRNA, but had lower level of superficial marker CD80 and transcriptional factor NF-κB, compared with pcDNA3-transfected control cells; the potential for stimulating the proliferation of allogenic lymphocytes was also decreased. Conclusion: Transient transfection of pcDNA3-HBV could decrease the function of DCs directly by inhibiting the maturation of DCs, which may be one of the mechanisms of immune tolerance in chronic B hepatitis.
    Distribution of lectin receptors in the mouse oviduct during the peri-implantation period
    HE Xiu-quan,LIU Kai,BING Lu-jun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  329-332. 
    Abstract ( 1079 )   PDF (240KB) ( 748 )   Save
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    Objective: To explore the distribution pattern and dynamic changes of lectin receptors in the mucosa of mouse oviduct during peri-implantation period. Methods: Using biotin labelled ECL, UEA and PHA-L as probes, we examined the distribution and dynamic changes of their receptors with affinity cytochemistry and image analysis. Results: The receptors of ECL, UEA and PHA-L all existed in the mouse oviduct, but their levels and distribution patterns were different. The level of the ECL and the PHA-L receptors as higher at the estrus than that at the diestrus. ECL and PHA-L receptors maintained the lower level at the day 4 and 5 of pregnancy than those at the diestrus. The level of UEA receptor at diestrus was much higher than that at estrus and that of pregnancy. Conclusion: During peri-implantation, the decrease of the ECL and the PHA-L receptors may lead to the failure of implantation in the oviduct, and the increase of the UEA receptors at diestrus may contribute to the process of fertilization.
    A multivariate and multilevel model in longitudinal data
    WANG Qi-wen,WANG Jie-zhen,XUE Fu-zhong,WANG Yan-mei,XU Ling-zhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  333-335. 
    Abstract ( 1383 )   PDF (211KB) ( 914 )   Save
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    Objective: To explore the applicability of a multivariate and multilevel model in a longitudinal research with several dependent variables. Methods: Influences of the independent variables (year, area and grade) on the two dependent variables (the total value of instruments and equipments in hospital and the operation income) and the relationship between the two dependent variables were assessed by the model. Results: Independent variables of year and hospital level, rather than area were associated with the increase of the two dependent variables. The two dependent variables positively correlated with each other. Conclusion: Multivariate and multilevel models can effectively analyze longitudinal data with several dependent variables.
    Ischemia preconditioning reduces ischemia/reperfusion injury of the hippocampal neurons by inhibiting the release of cytochrome c from mitochondria in rats
    LIU Ya-jun,LIU Ke-jing,XIE Dong-ping,XU Fu-yu,MIAO Bing,CHEN Lian-bi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  336-339. 
    Abstract ( 1086 )   PDF (250KB) ( 683 )   Save
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    Objective: To investigate the role of release of cytochrome c from mitochondria in the protective process of hippocampal neurons against ischemia/reperfusion injury by cerebral ischemic preconditioning (IPC) in rats. Methods: Rats were randomly divided into three groups: an ischemia/reperfusion group (IR), an ischemic preconditioning+ischemia/reperfusion group (IPC+IR), and the control group. Histological changes in the neurons of the hippocampal CA1 region were produced with HE staining. The apoptotic neurons were counted with TUNEL staining and the release of cytochrome c from mitochondria were determined with immunohistochemistry in the same brain area. Results: Compared with IR group, cerebral ischemic preconditioning can increase the number of survival neurons in hippocampal CA1 region(P<0.001), markedly reduce the number of apoptotic pyramidal neurons(P<0.001), and inhibit the release of cytochrome c from mitochondria. Conclusion: Cerebral ischemic preconditioning (IPC) can protect the hippocampal neurons against ischemia/reperfusion injury by reducing the release of cytochrome c from mitochondria and inhibiting the apoptosis.
    Structural changes of myocardium sleeves in canine pulmonary veins due to sustained atrial fibrillation
    MA Xiao,ZHANG Wei,YANG Guirong,ZHONG Ming,LI Li,ZHANG Yun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  340-342. 
    Abstract ( 1482 )   PDF (249KB) ( 823 )   Save
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    Objective: To study structure changes of myocardium sleeves in canine pulmonary veins(PVs) due to sustained atrial fibrillation(AF). Methods: The tissue and cell structure in individual veins was assessed by lightmicroscopy using HE and Masson′s Trichrome staining. Results: Compared with control dogs, sections from AF dogs showed cellular hypertrophy, disarranged cell bundles and increasing connective tissue. Some myocytes were surrounded by collagen tissue and myofilament was disrupted. Masson′s Trichrome stain showed that from the left atrium towards the vein, and the myocardial sleeves were gradually attenuated and ended in a rounded tip surrounded by fibrous tissue. Within myocardial sleeves, sharp transitions in fiber orientation were sometimes observed. It was apparent that myocytes on the endocardial side of myocardial sleeves were cut transversely in longitudinal sections. Samples from AF dogs showed, however, collagen tissue increased markedly and disrupted some areas. Conclusion: The special structure of the myocardial sleeve in PVs and its structural remodeling due to sustained AF may provide a substrate for forming micro reentry, which is an important mechanism for the occurrence and maintenance of AF.
    Effect of Oxylator and impedance threshold valve on coronary perfusion pressure during cardiopulmonary resuscitation in pigs
    ZHONG Jing-quan,Paul Dorian,GAO Hai-qing,ZHANG Wei,LI Li,ZHONG Ming,GE Zhi-ming,ZHANG Mei,JI Xiao-ping,WANG Rong,JIANG Gui-hua,ZHANG Yun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  343-347. 
    Abstract ( 967 )   PDF (270KB) ( 662 )   Save
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    To investigate the hemodynamics and coronary perfusion pressure(CPP) impacted by Oxylator and the impedance threshold valve (ITV) during cardiopulmonary resuscitation (CPR). Methods: ACD-CPR (active compression decompression CPR) was performed in twelve pigs in ventricular fibrillation (VF) models. After two minutes, each pig randomized to receive ITV, Manual, Oxyltor, and ITV combined with Oxylator during a three-minutes of ACD-CPR. Results: The CPP during CPR in Manual, Oxylator, ITV and ITV+Oxyaltor was (21.6±1.98)mmHg, (25.2±2.08)mmHg,(30.7±2.56)mmHg and (22.7±2.06)mmHg, respectively. There were no differences of left peak ventricular pressure and aortic peak pressure during compression phase of CPR in Manual, ITV, Oxylator and ITV+Oxyaltor. The cumulative successful defibrillation energy in ITV, Manaul, Oxylator and ITV+Oxyaltor was (430+57.1) J, (500±0) J, (860±0) J and (530±30.0) J, respectively. Conclusions: (1) ITV+Manual is the best for the CPP among the four episodes. (2) There are no significant differences of left ventricular and aortic peak pressure during compression phase of CPR among four episodes when ACD-CPR is used. The aortic mean pressure during CPR also is not significantly changed among four episodes. (3) ITV+Manual is the best to be defibrillated of the four episodes, probably because its CPP is the largest in this episode.
    Prodromal angina pectoris beneficial factors for patients with acute myocardial infarction
    LIU Li-ming,DING Shi-fang,WANG Qing,GAO Wei,ZHANG Yun,ZHOU Liao-sheng,LI Ji-fu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  348-352. 
    Abstract ( 1113 )   PDF (280KB) ( 795 )   Save
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    Objective: To explore the factors affecting short-term prognosis of acute myocardial infarction(AMI) patients with prodromal angina pectoris( AP).Methods: From October 2001 to December 2004, 234 AMI patients with previous angina pectoris(AP) (group A)and 255 AMI patients without AP (group B)were studied. In group A, 147 patients received intravenous thrombolysis( A1 group) and 87 received PCI( A2 group). In group B, 70 patients received intravenous thrombolysis (B1group) and 185 received PCI (B2 group). The clinical data and the presence of cardial death, left ventricle function failure, arrhythmia, recurrent myocardial infarction were analyzed. Result: ① The clinical and combined endpoint events were not different between A and B group; ② The peak creatine kinase (CK) and CK-MB[(2386±1271)IU/Lvs(3060±1241) IU/L, P<0.001;(166±94)IU/Lvs(198±127) IU/L, P<0.05] were lower, left ventricle function failure, recurrent myocardial infarction, cardial death and combined endpoint events were all lower in A group[10.9% vs 25.7%, P<0.01;6.8% vs 18.6%;44.9% vs 60.0%, all P<0.05]. Diabetes, hyperglycemia correlated with cardial dearth, arrhythmia, recurrent myocardial infarction (P<0.01、 P<0.05、 P<0.05 respectively ), the elder, coronary heart disease correlated with arrhythmia(P<0.05、 P<0.01 respectively) In A group. Multivariate Logistic regression indicated that left ventricle function failure Independent correlates with arrhythmia, recurrent myocardial infarction(OR: 14.9,CI:4.39-50.39, P<0.001; OR:26.4,CI: 15.73-121.64, P<0.01); cardial death is independently related with left ventricle function failure (OR: 84.0,CI :9.20-767.31, P<0.01); arrhythmia correlates with left ventricle function failure, hyperglycemia independently (OR: 14.71,CI:4.57-47.62, P<0.001; P<0.001); ③ The combined endpoint events were no difference between A2group B2 group. Conclusions: AP can reduce the combined endpoint events of AMI that received intravenous thrombolysis. But diabetes and hyperglycemia can counteract the profitable effect of AP in patients with AMI received intravenous thrombolysis. The elder、 coronary heart disease、 left ventricle function failure can decrease the profitable effect of AP in patients with AMI received intravenous thrombolysis.
    Relationship between gene expression of IL-1β and serum endotoxin in a animal model of cerebrogenic multiple organ dysfunction syndrome complicated by cerebral hemorrhage
    QU Chuan-qiang,MA Lin,GUO Hong-zhi,HE Yan,WANG Lei,LI Chun-hai
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  353-356. 
    Abstract ( 1230 )   PDF (290KB) ( 766 )   Save
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    Objective: To discuss the relationship between gene expression of IL-1βand serum endotoxin in a animal model of cerebrogenic multiple organ dysfunction syndrome(CMODS) complicated by cerebral hemorrhage, and to investigate the pathogenesis of CMODS. Methods: Ninty-six Wistar rats were randomly divided into four groups: normal control group (n=6), sham-operative group (n=6) and model group 1 with 0.4U collagenase (n=42) and model group 2 with 0.8U collagenase cerebral (n=42). The content of endotoxin in plasma was evaluated with a test box. Gene expression of IL-1β was assayed using in situ hybridization. The area density and the optical density of positive staining expressing IL-1βmRNA were analyzed for the relative content of IL-1βmRNA using in situ hybridization and CMIA medical imaging analysis system. Results: Expression of IL-1βmRNA in lung, liver, intestines andkidney tissues started to increase at 12 hours in model groups, and reached the peak at 24-36 hours. This was markedly higher in model group 2 than in model group 1 at 24 hours(P<0.01). Compared with normal control and shamoperative groups, expression of IL-1βmRNA was significantly higher at 12-48 hours in models groups(P<0.01). There was endotoxemia administration after acute cerebral hemorrhage. Plasma endotoxin level of model group 2 was higher than that of model group 1(P<0.01). It started to increase at 8 hours after hemorrhage andpeaked at 24 hours, then returned to the baseline value at 72 hours in model group 1, but still maintained high level in model group 2. Relative analysis indicated that there was significant positive correlation between the IL1βmRNA expression in lung, liver, kidney, intestine and plasma endotoxin level(P<0.01). Conclusion: Endotoxemia occurres after cerebral hemorrhage, and the gene expression of IL-1βincreases, which implicate that endotoxemia irritates the release of inflammatory factor, and systemic inflammatory response syndrom(SIRS) developes. This is key process in generation of CMODS.
    Effects of anti-epileptic drugs on the blood levels of homocysteine, folate and vitaminB12
    WANG Xiao-hua,ZAHNG Tong-xia,ZHAO Xiu-he,Gao Jing,LIU Xue-wu,CHI Zhao-fu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  357-359. 
    Abstract ( 1411 )   PDF (243KB) ( 1043 )   Save
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    Objective: To investigate the effect of antiepileptic drugs(AEDs) on the blood levels of homocysteine, folate and vitaminB12. Methods: There were 3 groups tested: ①88 epipleptic patients, taking a single AEDs; ②epileptic control group, 30 epileptic patients not taking AEDs; ③normal control group, 40 healthy controls. The levels of homocysteine, folate and vitaminB12 were determined by a fluorescence quantity method and an electrochemistry radiation immunity. Results: In epileptic patients taking a single carbamazepine (CBZ) and phenytoin(PHT), the levels of homocysteine were significantly higher and the levels of folate were significantly lower than those of the control group(P<0.001). The levels of homocysteine, folate and vitaminB12 were not significantly changed in patients taking valproic acid(VPA)(P>0.05). Conclusions: Homocysteine levels of epileptic patients taking CBZ and PHT were significantly increased whereas the folate levels of epileptic patients taking CBZ and PHT were signifacantly decreased. An adequate dosage of folate and vitaminB12 for epileptic patients is helpful in preventing angiocardiopathy and cerebrovascular diseases.
    Ethanol decreases the mRNA expression of the molecules involved in glucose metabolizing in the heart of rats
    HOU Xiao-lei,TAO Ying,FENG Li,WANG Lai-cheng,MA Chun-yan,WAN Qiang,GAO Ling,ZHAO Jia-jun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  360-364. 
    Abstract ( 1047 )   PDF (267KB) ( 762 )   Save
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    Objective: To observe the effects of chronic ethanol consumption at different doses on mRNA levels of IR, IRS1, IRS2, GLUT4, AMPK and MEF2 and to approach the possible mechanisms of ethanol on the glucose transportation. Methods: Forty-eight male Wistar rats, randomly divided into four groups, received either distilled water (control group) or ethanol of three different dosages: 5g/(kg·d) (high dose group), 2.5g/(kg·d) (middle dose group), and 0.5g/(kg·d) (low dose group). After treatment for 22 weeks, all animals were sacrificed and the left ventricles were quickly removed and saved for later analysis. The mRNA levels of IR, IRS1, IRS2, GLUT4, AMPKα1, AMPKα2, MEF2A and MEF2D were determined by the RT-PCR method. Results: Compared with the control group, all mRNA levels of IR, IRS1, IRS2, GLUT4, AMPKα1, AMPKα2, MEF2A and MEF2D were decreased in ethanol treatment groups (especially in high dose and middle dose group) (P<0.05). The correlation analysis indicated that GLUT4 had a significantly positive correlationship with IRS1,IRS2, AMPKα1, AMPKα2, MEF2A or MEF2D. Of them, it was closely associated between GLUT4 and IRS1 or MEF2A (P<0.01). Conclusions: Chronic ethanol consumption impairs the mRNA expression of IR, IRS1, IRS2, GLUT4, AMPKα1, AMPKα2, MEF2A and MEF2D of the cardiac muscle in rats.
    Changes of hepatic alpha-SMA and TIMP-1 induced by interferon-alpha-2b combined with ribavirin in patients with chronic hepatitis C
    LIU Zhi-rong,WANG Lei,LU Hui,YU Shu-li
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  365-368. 
    Abstract ( 1271 )   PDF (269KB) ( 825 )   Save
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    Objective: To investigate the changes of liver tissue inhibitors metalloproteinases-1(TIMP-1) and alpha-smooth muscle actin (α-SMA) induced by alpha-interferon-2b plus ribavirin therapy in patients with chronic hepatitis C. Methods: A total of 23 patients were enrolled in this study. All patients had received a 48-week interferon-alpha-2b plus ribavirin and had undergone virological follow-up for 24 weeks. In each patient, two liver biopsies had been performed: 1 week before treatment and 2 weeks after treatment. The patients were divided into two groups according to the viral response on 24 weeks after treatment: sustained viral response (SVR) group and non-SVR group. α-SMA and TIMP-1 in liver tissue were examined by immunohistochemical techniques and BI-2000 image-analysis system. Results: Posttreatment liver α-SMA and TIMP-1 were significantly lower than pretreatment scores(P<0.05) in all patients. The groups between SVR and non-SVR were compatible in pretreatment results(P>0.05). Posttreatment liver α-SMA and TIMP-1 were also significantly lower than pretreatment (P<0.05) in SVR patients. But we did not found the same consequence in non-SVR patients(P>0.05). There was a statistical difference between SVR and non-SVR patients in the changes of α-SMA and TIMP-1(P<0.05). Conclusion: Interferon-alpha-2b plus ribavirin has the ability of anti-fibrosis by reducing the expression of liver α-SMA and TIMP-1. The liver fibrosis is improved significantly in patients receiving SVR, and no worsens in non-SVR patients.
    Nanoparticles of diallyl trisulfide inhibit the growth of orthotopic transplanted hepatocellular carcinoma
    ZHANG Zhi-mian,YANG Xiao-yun,DENG Shu-hai,XU Wei,SHI Cheng-yao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  369-372. 
    Abstract ( 1151 )   PDF (304KB) ( 883 )   Save
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    Objective: To study the antitumor effect and the relevant mechanism of the hepatic targeted polybutylcyanoacrylate nanoparticles of diallyl trisulfide (DATS-PBCA-NP) on orthotopic transplanted HepG2 hepatocellular carcinoma in nude mice. Methods: The orthotopic transplantation HCC models were established by implanting HCC HepG2 xenograft bits under the envelope of the mice liver. Then, they were divided into 4 groups: the normal saline (NS) group, the empty nanoparticles group, the diallyl trisulfide (DATS) group, and the DATS-PBCA-NP group. Two weeks later, the tumor volume was determined, the PCNA expression in cancer tissues was determined by using immunohistochemistry, and the apoptosis and cell proliferation were determined by Western blot. Results: The successful rate of tumor orthotopic transplantation was 100%. Intravenous administration of DATS-PBCA-NP significantly delayed the growth of orthotopic transplantation hepatoma in nude mice (compared with the other three groups, all P<0.05). The results of immunohistochemistry and western blot analysis showed that tumors from DATS-PBCA-NP-treated mice exhibited a markedly down-regulating expression of PCNA compared with the control tumor. There was no significant difference in the expression of Fas and FasL proteins among the four groups (P>0.05). Conclusion: DATS-PBCA-NP has a significant antitumor effect on the orthotopic transplantation HCC model in association with the suppression of proliferation tumor cells.
    Effects of rhG-CSF and VEGF on cognition disorders of senescence accelerated mouse P10
    CONG Yan-bin,BI Jian-zhong,WANG Ping,WANG Yun,XIE Zhao-hong,ZHAO Yan-xin,WANG Xiao-yun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  373-375. 
    Abstract ( 1046 )   PDF (273KB) ( 808 )   Save
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    Objective: To observe the effects of rhG-CSF and VEGF on the cognition disorders of senescence accelerated mouse P10 (SAM-P10). Methods: Seventy-two SAM-P10 were randomly divided into control group, rhG-CSF group, and rhG-CSF combined with VEGF group. Mice in rhG-CSF group were subjected to 100μg/(kg·d) rhG-CSF subcutaeneouslly for 7 days, while mice in the rhG-CSF combined with VEGF group were first subjected to 50μg/(kg·d) VEGF intraperitoneally for 3 days and then 50?μg/(kg·d) rhG-CSF subcutaeneouslly for 7 days. Mice in the control group were subjected to normal saline. The Morris water maze test was applied before an administration and on the 7th, 14th, 28th, and 56th day after an administration to observe the changes of the cognitive ability. Results: The ability of learning and memory declined in the control group. Compared with the control group, the escaping latency declined and the percentage of swimming distance in the target quadrant increased both in the rhG-CSF group and in the rhG-CSF combined with VEGF group,moreover, the above items were better in the rhG-CSF combined with VEGF group than in the rhG-CSF group(P<0.05). Conclusion: rhG-CSF combined with VEGF is able to improve the cognition disorders of SAM-P10.
    Effect of all trans-retinoic acid on the proliferation and apoptosis of human Tenon's capsule fibroblasts in vitro
    DAI Li-hua,LI Wei-ning,QIAO Zhi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  376-379. 
    Abstract ( 1240 )   PDF (278KB) ( 822 )   Save
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    Objective: To study the effect of all trans-retinoic acid (ATRA) on the proliferation and apoptosis of human Tenon′s capsule fibroblasts(HTCFs) in vitro. Methods: Cell cycle distribution, the apoptotic rate and the expression of bcl-2 were determined by flow cytometry. Cell DNA was determined by agarose gel electrophoresis. Mitomycin C was used as a positive control. Results: Cell cycle distribution of HTCFs showed that cells of the G0/G1 phase increased and of the S phase decreased after the treatment with ATRA and MMC. Cellular apoptosis was decreased by ATRA at a lower dose (1×10-9mol/L), and the apoptotic rate was significantly increased in a dose-dependent manner. Expression of bcl-2 was significantly decreased by ATRA. A “ladder” strand of DNA was produced of the ATRA groups by DNA agarose gel electrophoresis. Conclusion: ATRA can significantly inhibit the proliferation of HTCFs and induce the apoptosis of HTCFs without apparent cytotoxicity.
    An animal model of recurrent seizures in the neonatal rat
    SHI Xiu-yu, WANG Ji-wen,LI Xing-xia,SUN Ruo-peng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  380-383. 
    Abstract ( 1204 )   PDF (292KB) ( 876 )   Save
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    Objective: To establish a kind of rat model of recurrent neonatal seizures, and evaluate its morphological and behavioral effects. Methods: The rats in this study were divided into experimental group (n=20) and control group (n=12). Neonatal Wistar rats (n=20) of experimental group were subjected to three times of pilocarpine injections intraperitoneally at postnatal day 1, 4 and 7. Neonatal rats (n=12) of the control group were given saline injection intraperitoneally at the same time. All rats were administered bromodeoxyuridine (BrdU) 36 hours before killed to label new born cells. Observe the rats′ behavioral and histological changes at different times. Results: With the increase of seizure number, the mean time to onset and the dosage of pilocarpine decreased, and the manifestations of seizures became intenser. No seizure and death were found in the control group. BrdU-labeled cells decreased significantly in the experimental group comparing with the controls at P7 (P<0.05), while at P52 BrdU-labeled cells increased significantly in the experimental group (P<0.05). There was no evident neuron loss in both the experimental and the control groups at P7 and P52. Conclusion: The established recurrent seizures model neonatal rat is reliable for investigating the temporary and chronic effects of neonatal seizures.
    Differentiation of bla-TEM genes in highly resistant Acinetobacter lwoffi strains
    YU Xiu-juan,YAO Ping,KONG Qing-lian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  384-388. 
    Abstract ( 1612 )   PDF (239KB) ( 815 )   Save
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    Objective: To discover the extended spectrum beta-lactamases (ESBLs) gene types and the characteristics of Acinetinbacter lwoffi strains which were highly resistant to most kinds of antibiotics and to compare the differentiation in nucleotide and amino acid sequences to show the possible influence in laboratory. Methods: Three strains of A.lwoffi with ESBLs phenomenon were isolated from patients′ sputum suffering from respiratory infection. The genes of blaTEM, SHV, OXA, IMP and CTX-M were determined by PCR. Nucleotide sequences were detected by ABI automated sequencer and analyzed by the DNA star software to compare the differentiation in nucleotide and amino acid sequences. Results: The bla-TEM gene type was determined in two strains, but the blaTEM, SHV, OXA, IMP and CTX-M genes were not determined in another strain. Amplified nucleotide sequences of TEM genes were 1012bp and 1007bp, respectively. The identical rate was 98.2%. There were 17 pairs different in them. The blaTEM gene, 832bp, coded for 277 amino acids. Among them, 8 amino acids were different, which mainly happened on Asp, Gly and Ser, substituted by His, Arg/Ala and Cys. They had pI value of 5.479 and 5.083, respectively. Conclusion: The blaTEM gene presented in A.lwoffi highly resistant strains in Shandong province. However, they produce some differentiations in nucleotide and amino acid sequences. These differentiations influence some conditions of laboratory method to pick up the resistant strains.
    Angio-architectural factors related to cerebral arteriovenous malformations hemorrhage
    WANG Zhi-gang,ZHANG Ji-qing,DING Xuan,JI Yong,HAN Lei,LI Tao,QU Chun-cheng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  389-392. 
    Abstract ( 1164 )   PDF (244KB) ( 738 )   Save
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    Objective: To explore the relationship between angioarchitectures of cerebral arteriovenous malformations(AVM) and hemorrhage and to reveal the risks of bleeding. Methods: Aortocranial angiography was taken for 200 patients by digital subtraction angiography(DSA), and then the feeding artery of AVM, the malformation vessel corps, and the draining veins were searched. Bleeding ratios of patients with different angioarchitectures were compared. Results: AVMs feeded by perforators, located in the basal ganglia and post cranial fossa, with small size, exclusively deep drainage, venous stenoses and complicated aneurysms were more likely to present with hemorrhage. Conclusion: The hemorrhage of AVM is significantly concerned with many factors, such as the type of feeding artery, size and location of the AVM, the draining vein and complicated aneurysms or not.
    I implantation combined with Gemcitabine in the treatment of advanced pancreatic cancer
    LI Yu-liang,WANG Yong-zheng,WANG Xiao-hua,ZHANG Fu-jun,ZHU Li-dong,ZHANG Wang-ming,LI Zheng,LI Zhen-jia,ZHANG Kai-xian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  393-396. 
    Abstract ( 1570 )   PDF (279KB) ( 745 )   Save
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    Objective: To study the role of 125〖KG*4/5]I implantation plus gemcitabine in treatment of advanced pancreatic cancer(APC). Methods: Fortythree untreated patients with APC were randomly divided into two groups: 125Results: Overall response rate(CR+PR)in Group A and in Group B was 61.1% and 32.0% respectively(P<0.05). Clinical benefit response was experienced by 72.2% of Group A compared with 40.0% of Group B(P<0.05). The survival rates of 3, 6, 12 and 18 months were100%, 94.4%, 72.2% and 61.1% for Group A and 100%, 76.0%, 32.0% and 12.0% for Group B (P<0.05). Conclusion: 125I plus GEM is effective in easing pain, improving the healthrelated quality of life,and prolonging the life of patients.
    Relationship between the autonomic dysreflexia and calcitonin gene-related peptide in rats following an acute spinal cord injury
    HU Kui,LIU Pei-lai,ZHANG Yuan-kai,DING Ming,ZHOU Lei,LI Ming
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  397-400. 
    Abstract ( 1105 )   PDF (292KB) ( 722 )   Save
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    Objective: To study the relationship between the autonomic dysreflexia(AD) and positive neurons of calcitonin gene-related peptide(CGRP) in rats following an acute spinal cord injury. Method: Changes of the AD and the dendrite of the CGRP positive neurons were produced at the day of 0, 3, 6, 9, 12, and 15 in Wistar rats following an acute spinal cord injury (SCI). Result: CGRP increased in a time-dependent manner by the quadratic function in 15 days. There was correlation between the AD and the restitution of the CGRP positive neurons. Conclusion: CGRP increases in a timedependent manner by the quadratic function. There are relationships between AD and CGRP positive neurons.
    Effects of valsartan and low molecular weight heparin on the growth of glomeruli mesangial cells stimulated by high glucose
    WANG Yun-shan,LIU Fang-e,JIAO Bo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  401-403. 
    Abstract ( 1333 )   PDF (201KB) ( 801 )   Save
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    Objective:To observe the effect of valsartan and low molecular weight heparin on the proliferation of glomeruli mesangial cells stimulated by high glucose. Methods:Mesangial cell line was treated by valsartan and low molecular weight heparin in different concentration and time, the proliferation activity was measured by MTT. Results:Both valsartan and low molecular weight heparin could inhibit the proliferation of mesangial cells significantly, and when combined the effect was intensified. Conclusion:The result suggests that the inhibition on mesangial cells is intensified when combined valsartan with low molecular weight heparin, compared with single dose.
    Changes of myocardial ultrastructure induced by grape seed proanthocyanidin extracts in STZ diabetic rats
    CHENG Mei,GAO Hai-qing,XU Ling,LI Bao-ying,LI Xian-hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  404-406. 
    Abstract ( 1183 )   PDF (272KB) ( 797 )   Save
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    Objective: To observe the effect of grape seed proanthocyanidin extracts (GSPE)on plasma advanced glycation end products (AGEs) and myocardial ultrastructures of streptozotocin(STZ) induced diabetic rats. Methods: Male Wistar rats were subjected to 0.1% STZ through the tail vein to induce diabetics.Then, they were randomly divided into two groups: diabetic model (DM) group (DM1,n=8)and GSPEtreatment diabetic group (DM2,n=12, 250?mg·kg-1·d-1). Nondiabetic Wistar rats were enrolled in other two groups: the control group(C1, n=10) and the GSPE treatment group(C2 group, n=10, 250?mg·kg-1·d-1). After 24 weeks, all rats were killed, and the fasting plasma glucose, glycosylated hemoglobin and AGEs were determined. Changes of myocardial ultrastructure were produced by an electronic microscope. Result: Plasma AGEs of DM2 group was lower than that of DM1 group (t=2.792,P=0.02). The myofibrillaes and the chondriosomees disordered in DM1 group, but changed after the GSPE treatment. Conclusions: GSPE can inhibit nonenzymatic reactions of glycosylation in STZ-induced diabetic rats and protect the myocardial ultrastructure.
    Mood,personality and copying styles in non-dialysis petients with chronic kidney disease
    LI Xian-hua,JIANG Hong,JIANG Bei,HU Zhao,PAN Fang,GAO Hai-qing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  407-411. 
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    Objective: To explore the role of negative mood, psychological health, personality in nondialysis patients with chronic kidney disease. Methods: Fiftyeight nondialysis patients with chronic kidney disease (based on K/DOQI) and thirtyone healthy persons were enrolled in this study. The general data, renal function, SAS, SDS, SCL90, copying styles and EPQRSC questionnaire were subjected to all. Result: Scores of the mood of depression and anxiety, negative copying of the experimental group were significantly higher than those of the control group(P<0.01). The neuroticism of the experimental group was significantly higher than that of the control group(P<0.01). Scores of omatization, sensitivity of interpersonal relation, depression, anxiety, hostility, fear, monomania, psychiatric and total scores, total average scores, positive numbers, positive average numbers of the experimental group were higher than those of the control group(P<0.01). Correlation analysis showed that age was positively correlated with the mood of depression and negative copying(P<0.05), educational level was negatively correlated with the mood of depression and anxiety, and serum albumin was negatively correlated with negative copying(P<0.05). Conclusion: The mood of depression and anxiety is an obvious symptom in nondialysis patients with chronic kidney disease. Psychological health of patients is obviously decreased than that of the controls. Age and educational level are associated with the mood of depression and anxiety, while renal function is not associated with the mood of depression and anxiety and the psychological health.
    Effect of the Yishenhuoxue Capsule on the growth factor of hyperhomocysteinemic rats
    YANG Rui-xue,ZHANG Ji-dong,ZHANG Wei-dong,JIA Qing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  412-415. 
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    Objective: To explore the therapeutic effect of the Yishenhuoxue capsule on basic fibroblast growth factor (bFGF) and transformation growth factorβ1 (TGF-β1) of hyperhomocysteinemia rats. Methods: Using randomized single blind method, 80 Wistar rats were divided into 8 groups: the model group, the normal group, the large dose of the Yishenhuoxue capsule group, the little dose of the Yishenhuoxue capsule group, the Western Medicine group (folic acid, VitB12, and VitB6), the Dahuangzhechong pill group, the preventive group and the blank group. The rats were induced hyperhomocysteinemia except for those in the normal group. The basic fibroblast growth factor(bFGF), transformation growth factorβ1 (TGF-β1) mRNA in aorta vessel were determined by the in situ hybridization. Result:Yishenhuoxue capsule could decrease the expression of bFGFmRNA(P=0.000), TGF-β1 mRNA(P=0.032) in hyperhomocysteinemia rats better than other medicines. Conclusion: Yishenhuoxue capsule could improve the endothelial cell function, reduce the part inflammation reaction of the part vessel, restrain the proliferation of vessel smooth muscle cells and endothelial cells, prevent the development of atherosclerosis.
    Effects of various treatments on bowel function after shigella flexneri infection in rats
    GUO Min,WANG Jing,WANG Fa-cheng,LI Nan,WEMG Yan,JIA Zhi-wei,LI Yan-qing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  416-419. 
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    Objective: To observe the changes of bowel sensory-motor function after shigella flexneri infection and the effects of various treatment for bowel function in rats, and therefore to provide guidance for preventing PI-IBS. Methods: Forty female Wistar rats weighted 170~180g were divided into normal control and model, antibiotics-treatment, probiotics-treatment and combination treatment groups. The 32 rats in the latter 4 groups were firstly infected with shigella flexneri by gavage to establish the model of acute infectious enteritis, and the normal controls were treated with 0.9% saline. Secondly in 16~22 days after bowel inflammation, the perceptual threshold was detected and the contraction of enteric smooth muscle was measured in vitro to observe the changes of bowel sensitivity and motility and the same time the histological changes were observed. Results: No obvious histological changes were found in different groups. The perceptual threshold decreased and the tension integral of enteric smooth muscle increased greatly in model group, antibioticstreatment and probioticstreatment groups(P<0.05), while that of combination treatment group had no difference from normal control. Conclusion: Shigella flexneri infection can lead to bowel sensorymotor disorder which prolongs for a period of time after inflammation diminishes. Treatment with antibiotics or probiotics singlely can not normalize the functional disturbance, but combination treatment can.
    Application of the direct digital dental radiography system to diagnose proximal caries in premolars
    LIU Mei,WANG Duo,WANG Xu-xia
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  420-422. 
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    Objective: To explore the clinical value of dental radiograph process in digital dental radiography system (DDRS)in diagnosing and treating the proximal caries. Methods: The patients, who were diagnosed as proximal caries with clinical exploration, were taken dental radiographs. The radiographs′ noises were processed and the radiographs were arranged in random order according to their computer number. One kind of image processing setup, following by another one assessed at least one week later, which could eliminate mutual obstruction, was examined jointly by three observers. Relative clinically diagnostic yields and four kinds of image processing setup were compared statistically. Results: Relative diagnostic accordance rate of the clinical exploration and four kinds of image processing setup was higher(P>0.01)in the first premolars. Relative diagnostic accordance rate of the clinical exploration and the pseudocolor image setup and negative image setup was higher(P>0.01), and that of the clinical exploration and the pseudo3D image setup and positive image setup was lower(P< 0.01), showing statistical differences in the second premolars. Conclusion: The computed image processing technique in DDRS can be applied to clinical examination of the proximal caries in premolars as an adjunct method.
    A Meta-analysis between the helicobacter pylori infection and gastric carcinoma
    AN Ning,LI Deng-xin,CHEN Tong,ZHANG Jian-ye
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(4):  423-426. 
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    Objective: To investigate the correlation between Helicobacter pylori infection and gastric carcinoma. Methods: According to the consistent of the test results, twenty-three articles of epidemiology referred were analyzed by the D-L method. Results: There was a significant correlation between gastric carcinoma and Helicobacter pylori infection(OR=2.72, 95%CI:1.91~3.87). There was a statistical significance between Helicobacter pylori infection with gland carcinoma and gastric non-cardia carcinoma(OR=2.72,3.94), and not a statistical significance between Helicobacter pylori infection with non-gland carcinoma and gastric cardia carcinoma(OR=1.55). Conclusion: There is significant correlation between gastric carcinoma and Helicobacter pylori infection.