Journal of Shandong University (Health Sciences)

Survival and differentiation of GFP transgenic mice-derived neuroepithelial cells after being transplanted into rat brains

WANG Jia-zeng,BAO Li-hua,SUN Jin-hao,LI Zhen-hua,ZHANG Jing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 649-654.

Abstract ( 1079 )

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Objective: To observe the survival and differentiation of cells derived from the embryonic neural tube of green fluorescent protein (GFP) transgenic mice after being transplanted into the striatum of rats. Methods: Neuroepithelial stem cells derived from the embryonic neural tube of GFP transgenic mice were cultured and depurated in free serum medium containing B27, bFGF and EGF, and then they were directionally transplanted into the striatum of rats. Animals were sacrificed at different time points after being transplanted and frozen sectioned, and staining by MAP-2, GFAP, and TH was determined by immunohistochemical techniques. Results: Neuroepithelial stem cells derived from the embryonic neural tube could expand into neurospheres in the free serum medium and express nestin. The grafted regions were clearly visible, and the transplanted cells survived well and some of them differentiated into TH-positive cells. Conclusion: Embryonic neuroepithelial stem cells from GFP transgenic mice could be generated in vitro, survive and differentiate into neurons, especially dopaminergic neurons and neuroglia cells after being transplanted into rat brains.

Methylcyclopentadienyl manganese tricarbonyl injuries in PC-3M cells

QIN Wen,WANG Li-xiang,ZENG Ji-ping,CHEN Wei-wen,ZHANG Ya-lun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 655-657.

Abstract ( 910 )

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Objective: To explore the effect of methylcyclopentadienyl manganese tricarbonyl (MMT) on PC-3M cells. Methods: The MTS method was used to determine the time and concentration curves induced by MMT on PC-3M cells. Spectrophotometry was used to determine the changes of ROS, LDH and MDA and the activity of SOD. Results: The survival viability of cells decreased with MMT in a dose-and concentration-dependent manner. After induction by 2mmol/L MMT for 24h, the survival rate of PC-3M cells was 48.55%（P＜0.01）. In this process, the ROS, LDH and MDA obviously increased（P＜0.01）, and the activity of SOD significantly decreased（P＜0.01）. Conclusion: MMT injures PC-3M cells.

Application of a multilevel model in longitudinal data

WANG Yan-mei,WANG Jie-zhen,DING Shou-luan,XUE Fu-zhong,SUN Xiu-bin,WANG Qi-wen,XU Ling-zhong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 658-661.

Abstract ( 947 )

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Objective: To explore the applicability of a multilevel model in longitudinal data. Methods: The income of 45 hospitals with different grades for 8 years in Shandong and Henan Provinces was assessed by a multilevel model. Results: The multilevel model showed that differences of the hospital professional income and velocity and acceleration of increase were not significant between the two provinces, but they were significant in various grades. Conclusion: Multilevel models can efficiently analyze longitudinal data with hierarchical structure and missing data.

Expression and sigificance of COX-2 and VEGF in condyloma acuminate

LI Li-na,WANG Xu-zhou

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 662-664.

Abstract ( 898 )

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Objective: To investigate the role of COX-2 and VEGF in cell proliferation and angiogenesis of condyloma acuminate（CA）. Methods: Expression of COX-2 and VEGF in 40 cases of CA and 15 normal prepuce skins were determined by immunohistochemistry staining (SP). Results: Positive expression rate of COX-2 and VEGF in CA was 62.5% and 92.5%, respectively ,which was significantly higher than that of the controls （P＜0.05）. Expression of COX-2 and VEGF was strongest throughout the epidermis, but was weak in the basal layer in the control group. Expression of COX-2 was closely related to that of VEGF（P＜0.05）. Expression of COX-2 and VEGF had no correlation with the course of CA(P＞0.05). Conclusions: COX-2 and VEGF simultaneously promote the pathogenesis of CA. It may be an important method for CA by inhibiting the expression of COX-2 and VEGF.

Peroxisome proliferatorsactivated receptor α play a critical role in the inhibition of myocardial fibrosis induced by AngⅡ in vitro

HOU Xiao-yang,BU Pei-li,ZHANG Yun,FENG Jin-bo,LIU Chun-xi,LI Chuan-bao,HAO Ming-xiu

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 665-668.

Abstract ( 983 )

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Objective： To explore the effect of peroxisome proliferators-activated receptor α on myocardial fibrosis induced by angiotensinⅡin vitro. Methods： Cardial fibroblasts (CFs) of neonatal Wistar rats were isolated and cultured, and were stimulated with AngⅡ. CFs proliferation was measured by thiazolyl blue（MTT）assay. mRNA was measured by reverse transcriptionpolymerase chain reaction(RT-PCR). Results： Compared with the control group, after a 12-hour treatment with AngⅡ, mRNA expression of collagen I and TIMP-1 in CFs was increased, but MMP-2 was significantly decreased while the MTT value of CFs was increased. Bezafibrate, a kind of PPARα activators， inhibited the above changes in a dose-dependent manner except for the MTT value, of which no significant changes were produced. MK886, a kind of PPARα antagon, abrogated the action of Bezafibrate. Conclusion： The results suggest that the PPAR αdependent pathway is critically involved in the inhibition of myocardial fibrosis in vitro.

Correlation between pulse wave velocity and ankle-brachial index and cardiovascular risk factors in elderly patients

LI Bao-ying,GAO Hai-qing,LI Xiao-li,MA Ya-bing,LIU Yuan-ping,WANG Min

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 669-672.

Abstract ( 1022 )

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Objective: To obtain reliable data on the epidemiology, co-morbidities and risk factor profile of large artery arteriosclerosis and peripheral arterial disease (PAD), the clinical significance of brachial-ankle pulse wave velocity (baPWV) and ankle-brachial index (ABI) were evaluated as indicators of arteriosclerosis and PAD in aged patients. Methods： One hundred and ninety-one elderly patients (64.4% male, 71.73 years old in mean) were recruited for this study. baPWV and ABI were measured by the VP-1000 automatic arteriosclerosis measurement system. Results: One hundred and ten elderly patients (57.6%) were found to have large artery arteriosclerosis. The risk of large artery arteriosclerosis in elderly patients became higher with an increase of age (OR=1.060, 95% CI=1.010-1.113). Gender (OR=2.494, 95% CI=1.067-5.825) and history of hypertension(OR=10.569, 95% CI=4.74823.527) were independently associated with high baPWV (P＜0. 05). Moreover, 36 elderly patients (18.8%) were found to have PAD. The risk of PAD in elderly patients was related to a history of diabetes mellitus(OR=4.383, 95%CI=1.974-9.733, P＜0.001). Conclusion: BaPWV and ABI measurements are sensitive and independent indicators for evaluating the degree of arteriosclerosis and occlusion in the aged, which can effectively evaluate cardiovascular risk factors in elderly patients.

Hybridization detection of mutation sites in hepatitis B virus using oligodeoxynucleotide and PNA array

LU Yan-qin,HAN Jin-xiang,SHEN Zhong-lin,ZHU Bo

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 673-676.

Abstract ( 1065 )

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Objective: To compare the oligonucleotide array and PNA array in determining mutation of HBV. Methods: Oligonucleotide and PNA probes which targeted 1762A-T and 1764G-A, 1858C-T and 1896G-A mutations and wild sites of HBV were designed and immobilized on aldehyde modified glass slides after synthesis. Target DNA was obtained by asymmetric PCR with a tamara-labeled upper primer. Result was produced by scanning and quantifying analysis after hybridization with labeled target DNA. Meanwhile, the PCR product was sequenced to analyze three mutation sites. Results: The ratio of fluorescence signals for 1762-1764 double site, 1858 and 1896 sites (wild type: mutation type) was 1.44, 1/1.24 and 1.05 in the oligonucleotide array and 2.8, 1/3.02, 1/1.71 in the PNA array. 1858T mutation and 1762A-1764G, 1896G wild sites were found in DNA sequencing. Conclusions: The oligonucleotide array and the PNA array could successfully detect the three mutation sites of HBV, which is in accordance with the result of DNA sequencing. PNA shows a high priority in attaching the target DNA and identifying the single-base mismatch.

Double embolization and pericardial devascularization with splenectomy in the treatment of portal hypertension: a comparative study

FENG Kai,ZHANG Chun-qing,LIU Fu-li,XU Hong-wei,WANG Jing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 677-680.

Abstract ( 1204 )

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Objective: To explore the effect of percutaneous embolization of gastroesophageal varices, with TH glue as the embolus, combined with partial splenic embolization(PTVE+PSE) and pericardical devascularization plus splenectomy in the treatment of portal hypertension. Methods: A total of 147 cases were divided into two groups: 75 cases in the intervention group (PTVE+PSE) and 72 cases in the operation group. The incidence of re-bleeding, death, gastroesophageal revarices, the improvement of splenemagly and the complications were observed in the two groups. Results: All procedures and operations were successful. At the beginning of the five-year follow-up, there were no differences in the mortality and re-bleeding, and the improvement of splenemagly was remarkable in both groups. With time, the rate of re-bleeding and gastroesophageal variceal in the operation group was higher than that of the intervention group. Also, the complications in the operation group were more serious than that in the intervention group during perioperation, which increased the cost and time in hospital. Conclusion: With TH glues as the embolus, PTVE combined with PSE is safe, reasonable and effective for gastroesophageal varices. This treatment is able to replace pericardial devascularization plus splenectomy.

Effects of lamotrigine on the electroencephalogram and the expression of Bax and Bcl-2 in epileptic rats

XU Hong,SUN Chao-yan,YANG-Jia,XU Guo-wei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 681-684.

Abstract ( 1032 )

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Objective： To explore the effect of lamotrigine (LTG) on the behavior and electroencephalogram (EEG) of epileptic rats induced by penicillin and to explore the protective effect of LTG on the neurons of the hippocampus. Methods： Seizures in the group of epileptic rats induced by penicillin, the group of the LTG, and the group of the sodium valproate were measured, and the EEG was recorded for the groups. Immunohistochemical staining was used to observe the expression of Bcl-2 and Bax. Results： The epileptiform discharges were decreased in the group of the LTG and the group of the sodium valproate. The latencies and durations of seizures produced a significant difference between the group of LTG and the group of sodium valproate（P＜0.01）; Compared with the epileptic group induced by penicillin, Bcl-2 positive cells increased and Bax positive cells decreased in the group of LTG and the group of sodium valproate (P＜0.01). Compared with the group of sodium valproate, Bcl-2 positive cells in the group of LTG increased (P＜0.01). Conclusions： LTG is better than sodium valproate in anti-epilepsy, and it can protect the hippocampal neurons in epileptic rats induced by penicillin.

Primary culture and identification of human glomerular mesangial cells

LIU Hai-ying,GUAN Guang-ju,ZHANG Yan,LIU Gang,CHENG Bin,LI Xue-gang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 685-687.

Abstract ( 1556 )

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Objective: To isolate and identify the glomerular mesangial cells in vitro. Methods: Kidneys were isolated from the normal kidney tissues which were separated from kidney tumor patients and fetal kidneys after induction of labor with water bag, then glomerular mesangial cells were separated by using three-layer micropore fillers and they were subsequently characterized by a morphology method and an immunohistochemical method using flourescein isothiocyanate staining as a label for keratin and desmin. MTT and flow cytometry technology were used to determine the growth of mesangial cells. Results: Glomerular mesangial cells were characterized by their morphology and immunohistochemical character, and those cultured from fetal kidneys can be subcultured after 8 to 10 days and 7 to 10 passages in culture while from adult kidneys they can only be sub-cultured after 15 to 16 days and the fourth passage cells presented a poor growth mode. Conclusion: The isolated and cultured cells in vitro are glomerular mesangial cells and they are easier to retain in young kidneys than in adult kidneys.

The role of noninvasive hemodynamic monitoring in evaluating dry weight following hemodialysis

LIU Jin-yan,ZHU Bin,LI Su,WANG Xiao-ping,LIU Zi-dong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 688-691.

Abstract ( 1125 )

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Objective: To evaluate the diversity of thoracic fluid content (TFC) in dialysis patients before and after HD using the noninvasive hemodynamic monitor and to investigate its clinical application in modulating a suitable dry weight (DW). Methods: Thirty-seven patients were selected and divided into two groups based on their blood pressure: 18 patients with controlled hypertension (group A) and 19 patients with hypertension that was difficult to control (Group B). Sixteen healthy volunteers were enrolled in the control group (group C). A noninvasive hemodynamic monitor was utilized to determine the variation of thoracic fluid content (TFC), weight, systolic blood pressure (SBP), diastolic blood pressure (DBP) and ultra-filtration volume in patients before and after HD. The dry weight and volume of ultra-filtration were gradually modulated when TFC levels were above normal. Patients were re-evaluated with a noninvasive hemodynamic monitor 4 weeks after the initial assessment. SPSS software was used to analyze the data. Results: (1) The TFC level was significantly higher in groups A and B than in group C before HD (P＜0.05), and was dramatically reduced following HD in groups A and B. The TEC level in group B was still significantly higher than in group C. However, it decreased to normal after the dry weight and ultrafiltration volume were modified. The DBP/SBP were significantly reduced (P＜0.05) after the initial HD and eventually returned to a normal range 4 weeks after HD. (2) The post-HD TFC level in group B correlated well with the reduced mean arterial pressure (P＜0.01). The difference of the reduced TFC levels between the initial post-HD and post-HD after modification correlated well with the decreased BP post-HD (P＜0.01). Conclusion: The noninvasive hemodynamic monitoring method can be effectively used to evaluate the volume state in patients requiring HD, and has a significant clinical value in determining a suitable dry weight for these patients.

Pathological characters of the bronchial mucus membrane and inflammatory cell changes of BALF in mild and moderately persistent asthmatic patients

SU Li-li,ZHANG Xian-xin,YI Hong-li,ZHANG Meng,LI Huai-chen

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 692-696.

Abstract ( 1184 )

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Objective: To study the pathological characters of the bronchial mucus membrane in mild and moderately persistent asthmatic patients and to explore the possible functions of neutrophils. Methods: Bronchial biopsy specimens and bronchial alveolar lavage fluids(BALF) were obtained from 12 patients with intermittent asthma(group A), 16 patients with mild or moderately persistent asthma(group B) and 14 patients with peripheral lung cancer in an early stage (group C). The pathological characters were observed by light microscopy using HE staining. Expression of MPO in the bronchial mucus membrane was determined by immunohistochemical staining. The cell count and MPO, ECP, IL8 contents in BALF were determined at the same time. Results: The Eos count in BALF of the A, B and C groups（×106/ml）was （7.58±4.42）,（8.89±4.25） and（4.32±2.13）respectively, which was significantly higher in groups A and B than in group C(P＜0.05）. The neutrophil count was （4.04±2.14）,（15.03±6.79） and（4.15±1.09）respectively, which was significantly higher in group B than in groups A and C（P＜0.05）. The ECP content was （72.50±28.27）(mg/ml),（76.88±22.85）(mg/ml) and（42.67±24.00）(mg/ml) respectively, which was significantly higher in groups A and B than in group C（P＜0.05）. The MPO content was（11.35±5.87）(u), （25.31±15.71）(u) and（12.67±6.44）(u)respectively, which was significantly higher in group B than in groups A and C（P＜0.05）. The IL8 content was （3.43±1.46）(ng/ml), （14.12±11.41）(ng/ml) and （2.93±0.93）(ng/ml)respectively, which was significantly higher in group B than in groups A and C（P＜0.05）. The MPO positive cells under the mucus membrane in group B were significantly higher than in group A（P＜0.05）. Conclusion: Both neutrophils and Eos participate in the inflammatory reaction of mild and moderately persistent asthma and neutrophils play a more important role in it.

Comparison of two methods in the isolation of human placenta mesenchymal stem cells

LI Dong,WANG Guo-yun,JU Xiu-li,SHI Qing,HOU Huai-shui

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 697-701.

Abstract ( 1167 )

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Objective: To investigate the protocols of isolating mesenchymal stem cells from the placenta and to evaluate their biological characters. Methods: The explant method and enzyme digestion method were used to isolate mesenchymal stem cells from the placenta. Morphology, immunotyping, growth kinetics and mutitopent differentiation of these cells were assayed. Results: FACS and differentiation experiments showed immunotyping of adherent cells isolated by the enzyme digestion method matched with the character of mesenchymal stem cells and could differentiate into osteoblasts, chondrocytes and adipocytes under an appropriate induction condition. Cells isolated by the explant method weakly expressed CD29 and CD105 and could differentiate only into adipocytes. Conclusion: The enzyme digestion method can effectively isolate mesenchymal stem cells with a high proliferative ability and multipotent differentiation ability.

Correlation between levels of IL-12 and IFN-γ and recurrent spontaneous abortion

WANG Guo-hua,ZUO Chang-ting,WANG Li-jun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 702-704.

Abstract ( 1056 )

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Objective: To explore the correlation between levels of interleukin-12(IL-12) and interferon-γ(IFN-γ) and recurrent spontaneous abortion (RSA). Methods: Thirty patients with RSA( the RSA group), twenty-five normal early pregnant women (the NEP group) and twentyfive normal non-pregnant women( the NNP group) were enrolled in this study. The serum concentrations of IL-12 and IFN-γwere determined by enzyme-linked immunosorbant assay(ELISA). Results: Levels of IL-12 and IFN-γ were significantly higher in the RSA group than in the other groups(P<0.05) and there was a positive correlation between IL-12 and IFN-γ. Levels of IL-12 and IFN-γ were lower in the NEP group than in the NNP group, but there was no statistical difference(P>0.05). Conclusion： IL-12 and IFN-γ may play an important role in the occurrence of RSA.

Correlation between serum TPA and sVCAM-1 and pathogenesis of pre-eclampsia

ZHANG Xiang-li,LIU Feng-ying

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 705-707.

Abstract ( 1064 )

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Objective： To explore the correlation of serum tissue polypeptide antigen（TPA）and the soluble vascular cell adhesion molecule-1(sVCAM-1) and pathogenesis of pre-eclampsia. Methods： ELISA was used to determine the serum of TPA and sVCAM-1 in pre-eclamptic(the experimental group) and normotensive pregnant( the control group) women, and the correlation of pre-eclampsia (PE) and neonatal outcomes was analyzed by rank correlation. Results： (1) Concentration of serum TPA in the experimental group was obviously higher than that in the control group (P＜0.05) and it was the highest in patients with serious pre-eclampsia（P＜0.01）. (2) Concentration of serum sVCAM-1 in the severe experimental group was obviously higher than that in the mild experimental and the control groups (P＜0.01). (3) There was a significantly positive correlation between the TPA and the sVCAM-1 in the experimental and control groups. (4) There were a significantly positive correlation between the TPA and sVCAM-1 and the DBP and urine protein in the experimental group, and a significantly negative correlation between the TPA and the sVCAM-1 and the weight of newborns. Conclusion： With the state of the disease worsening, serum TPA and sVCAM-1 is increased, indicating that the apoptosis of the trophoblast cells and the dysfunction of the vascular endothelial cells are aggravated.

Correlations between the blood motilin and gastrin levels in premature infants

WANG Yin,ZHU Wei-wei,LUO Huan-hua,JI Chao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 708-710.

Abstract ( 1033 )

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Objective: To explore the clinical significance of gastrointestinal hormone secretion in premature infants and premature infants with hypoxic-ischemic brain damage(HIBD), and to observe the therapeutic effect of erythromycin in small doses on premature infants with a feeding intolerance. Methods: Fifty premature infants including 23 with HIBD and 27 without HIBD (18 of the 27 with a feeding intolerance) were enrolled in this study. Twenty normal newborns were the control. The plasma motilin and serum gastrin levels were determined by radioimmmunoassay (RIA). The 12 infants with a feeding intolerance were subjected to 3-5mg/(kg·d)erythromycin. Results: ① Compared with the control and the premature infants without HIBD group, the blood MTL and GAS levels of premature infants with HIBD were higher(P＜0.01 ) . ②The blood MTL and GAS levels of premature infants without HIBD were lower than those of the control group(P＜0.05). There was a significant difference between the premature infants with a feeding intolerance and the premature infants without a feeding tolerance (P＜0.05), while there were not significant differences between the premature infants without feeding intolerance and the control group (P＞0.05). ③The effect of erythromycin was significantly different between the interventional group and the control group (P＜0.05). Conclusions: Premature infants have abnormal secretions of plasma motilin and serum gastrin. The blood level of MTL and GAS has a relationship to feeding intolerance and erythromycin therapy has a good effect on premature infants with feeding intolerance.

COX-2 expression in rectal adenocarcinoma and its relationgship with lymphangiogenesis and lymph node metastasis

WANG Jing-yuan,FU Qin-ye,DING Yin-lu,LIANG Si-feng,ZHANG Jian-liang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 711-713.

Abstract ( 987 )

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Objective: To investigate the expression of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor-C (VEGF-C) in human rectal adenocarcinoma． Methods: COX-2 and VEGF-C expressions were determined in 40 cases of rectal adenocarcinoma and 40 cases of normal rectal mucous membrane by the immunohistochemical PV-8000 two-step staining method． Lymphanigogenesis was evaluated by immunohistochemical staining with the specific antibody, VEGFR-3． Results: Expression rate of COX-2 and VEGF-C was 62.5%(25／40) and 65.0%(26／40)， respectively in 40 rectal adenocarcinoma specimens． VEGFR3 was positive in 21 cases（21/40， 52.5%）indicating lymphangiogenesis in the tumors．Expression of COX-2 was significantly correlated with that of VEGF-C， tumor lymphangiogenesis and lymphatic metastasis and serosa invasion（P＜0.05)， however, it did not correlate with the tumor size， location and histological typing（P＜0.05）． Conclusions: In rectal adenocarcinoma， expression of COX-2 is significantly associated with VEGF-C， lymphangiogenesis and lymphatic metastasis． COX-2 may up-regulate the expression of VEGF-C， which induces lymphangiogenesis and contributes to lymphatic metastasis．

In vivo vaccine effect and tumorigenicity of human breast carcinoma cells transfered by interleukin-18 gene

HAN Ming-yong,LIU Qi,TANG Bu-jian,DENG Yan,CAO Ming-feng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 714-717.

Abstract ( 966 )

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Objective: To study the effect of the interleukin-18 gene transfection on tumorigenesis of the human breast carcinoma cell line Bcap37. Methods: Bcap37 cells were transfected with a mammalin expression vector containing human IL-18 complementary DNA. The biological expression of IL-18 was determined by the RT-PCR and ELISA methods. Nude mice were injected with Bcap37 cells with or without the IL-18 gene. Results: The IL-18 cDNA was successfully integrated into the Bcap37 cells. There were (113.8±4.7)pg IL-18 secreted by one million transduced cells in 24 hours. In vivo tumor growth curves, based on measured tumor size, Bcap37, Bcap37-pcDNA3.1 and Bcap37-IL-18 resulted in tumor formation from 8 to 11 days after injection. The growth rate of Bcap37-IL-18 in vivo was significantly slower than that of Bcap37 and Bcap37-pcDNA3.1 cells. Tumor formation by parental Bcap37 cells was significantly delayed in mice that had been immunized with Bcap37-IL18 irradiated cells. Conclusion: Human breast carcinoma cells were successfully modified by IL-18 cytokine genes and reduced the tumorigenicity. Breast cells transduced with IL-18 genes might be used as human colon cancer vaccines.

An experimental study on the attachment and growth of human skin fibroblast on a porcine collagenic dermal matrix in vitro

ZHANG Ai-jun,SONG Guo-dong,CAO Cheng-bo,WANG Yun-shan,BIAN Dong-hui

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 718-721.

Abstract ( 907 )

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Objective: To observe the attachment and growth of fibroblasts on a new porcine collagenic dermal matrix（PCDM） and investigate a method to improve the cell compatibility of the porcine dermal scaffold. Method: The fibroblasts were seeded on two sides of the PCDM. 1, 7 and 14 days later， the composites were respectively observed by HE staining, scanning electron microscope（SEM）and transmission electron microscope（TEM）. Results: Fibroblasts attached better on the papillary dermis than on the reticular dermis in the early period, but more samples of the reticular dermis demonstrated fibroblast stratified structure and infiltration below the cellseeded surface than the papillary dermis. Conclusion: The new PCDM has good compatibility with human fibroblasts in vitro. It is the most similar to human skin. Modification of the processing technology and removal of the papillary dermis is capable of improving the cell compatibility of the PCDM.

Surgical treatment of distal radius fractures

LIU Pei-lai,LI Ming,ZHANG Yuan-kai,DING Ming

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 722-725.

Abstract ( 1205 )

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Objective: To evaluate the effect of surgical treatment on distal radius fractures. Methods: Sixty-seven patients with distal radius fractures were subjected to surgical treatment, including fixation with a plate in 33 patients, with a K-wire in 14 patients, with external fixation in 12 patients and with external fixation combined with a K-wire in 8 patients. The final anatomical results were scored based on Sarmiento′s criteria, and the functional results were valuated based on the criteria of Fernandez. Results: All the cases were followed up for an average of 10 months (8 to 12 months). Based on Sarmiento′s criteria, the results were excellent in 31 cases， good in 28 cases，fair in 5 cases， and poor in 3 cases． The good-excellent rate was 88%. Based on Fernandez criteria， the results were excellent in 28 cases， good in 29 cases， fair in 7 cases， and poor in 3 cases． The goodexcellent rate was 85%. All the fractures healed in 2 to 6 months (averaging 3 months). Conclusion: A suitable surgical method for the un-expectant treatment patients is effective.

Pharmacokinetics and bioequivalence of paeonol capsules and tablets by the HPLC method

WU Jing,WANG Ben-jie,WEI Chun-min,KONG Xiang-lin,GUO Rui-chen

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 726-729.

Abstract ( 1058 )

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Objective: To establish the HPLC method for determination of paeonol in human plasma and to evaluate the pharmacokinetic parameters and the bioequivalence of its capsules and tablets in healthy Chinese volunteers. Methods: A liquor of plasma was collected at scheduled time points before and after a single dose of 160mg paeonol was orally given to 20 healthy volunteers in a two-way cross-over design test. The plasma samples were extracted with 300?μl acetonitrile. The paeonol concentration in plasma was determined by HPLC method using a XB-C18(250mm×4.6mm,5μm) column as a stationary phase, and THF-methanol-water-phosphonic acid(6∶60∶34∶0.1,V∶V)as a mobile phase. The pharmacokinetic parameters were determined and the bioequivalence of paeonol capsules and tablets was evaluated with DAS 2.0. Results: The limit of detection for paeonol was 10ng/ml, and a linearity obtained in the range of 10～500ng/ml was excellent（r=0.9998）.The relative standard deviations of intra-day and inter-day determination were less than 13.72%. The main pharmacokinetic parameters of paeonol after a single oral dose of 160 mg paeonol capsules and tablets were as follows: t1/2(h)1.03±0.35 and 1.09±0.62, Tmax(h)1.02±0.13 and 1.03±0.15， Cmax(ng/ml) 116.39±45.57 and 111.16±41.24，AUC0～3(ng·h/ml) 173.91±45.41 and 1.26±42.63，AUC0-∞(ng·h/ml) 217.13±56.55 and 220.27±67.24, respectively. The relative bioavailability of paeonol capsules was （101.56±9.31）%. Conclusion: The established HPLC method is highly sensitive and accurate, and can be successfully used in the determination of plasma paeonol and is its pharmacokinetics studies. The pharmacokinetic parameter of paeonol shows no significant differences between capsules and tablets. The two preparations are bioequivalent.

Quality of life of asthma patients and its influencing factors

DUAN Xi-ming,LIU Chun-hong,SHI Shou-sen,LI Yu,PAN Fang,CHEN Zheng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 730-734.

Abstract ( 1053 )

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Objective： To explore the quality of life(QOL) of asthma patients and its systematical influencing factors. Methods： 42 asthma patients and 35 normal people were tested with psychological rating scales including AQLQ, IDA, PANAS, SES, SQ. Results： The scores of asthma patients were higher than those of the control group in depression mood and negative affect. Compared with the normal controls, the self-esteem and safety-sense of relationship in patients were low while the emotional loneliness was high. The QOL of asthma patients had a negative association with vocation, the course of disease internal irritation and negative cognition concept and had a positive association with the degree of education, FEV1, positive affect, selfesteem, and asthma knowledge. The QOL had a significant negative association with depression mood and negative affect. Depression mood had a negative association with asthma knowledge and positive cognition concept, while it had a positive association with negative cognition concept. Internal irritability had a positive association with negative cognition concept. Negative affect had significant association with negative cognition concept. Selfesteem had a positive association with positive cognition concept. Emotional loneliness had a significant negative association with asthma knowledge. The safetysense of relationship had a positive association with positive cognition concept. The controlling-sense had a significant negative association with negative cognition concept. Conclusion： There are psychological problems in asthma patients whose QOL is influenced by many factors. Psychological intervention should be reinforced; asthma knowledge and positive cognition concept should be developed in asthma patients to improve their QOL.

A random control study on the attachment of children with attention deficit-hyperactivity disorder

LI Yun,XIA Ying,ZHANG Hong-jing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 735-738.

Abstract ( 1136 )

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Objective: To investigate the features of attachment in children with attention deficit-hyperactivity disorder(ADHD) and of children with different clinical subtypes of ADHD. Methods: The Attachment Security Scale was used for 89 children with ADHD(DSM-Ⅳ criteria) and 150 normal children. Children with ADHD were divided into three groups: the ADHD-I group (n=47), the ADHD-H group (n=10) and the ADHD-C group(n=32). Results: Scores of mother-dependence, father-dependence, mother-intimacy, father-intimacy, mother-child attachment, and father-child attachment of the ADHD children were all lower than those of the controls(P＜0.01). In the three subtype groups, scores of mother-dependence and mother-child attachment in the ADHD-C group were lower than those in the ADHD-H group (P＜0.05). Scores of the father-dependence, father-intimacy, and father-child attachment in ADHD children were higher than mother-dependence, mother-intimacy, and mother-child attachment(P＜0.05). Conclusion: Attachment security of ADHD children is lower than that of the controls. The mother-child attachment security in the ADHD-C group is the worst. The father-child attachment security is lower than the mother-child attachment security in ADHD children.

Effect of the Shumai capsule on apoptosis in rats with myocardial ischemia

YIN Hui-qiu,ZHANG Ji-dong,QIAO Yun,WANG Rong,WANG Bo,LIU Fen-ye

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 739-742.

Abstract ( 1049 )

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Objective: To explore the effect of the Shu-mai capsule (SMC) on apoptosis in rats with myocardial ischemia (MI). Methods: Rats with a ligated left anterior descending coronary artery (MI model) were randomly divided into the high and low dose of the SMC groups (SMCH and SMCL), the Dan-shen drop pill group（DSP）and the MIR group. A sham-operation group (Sham) was also enrolled in this study. Four weeks later, the ratio of apoptotic to normal cells, the expression of bax and bcl-2 protein, and the ultramicrostructure of the myocardium were respectively determined by terminal deoxynucleotide transferase-mediated dUTP nick-end labeling (TUNEL) assays, immunohistochemical staining, and transmission electron microscopy（TEM）. Results: The SMC can reduce the expression of bax, increase the expression of bcl-2 and reduce the apoptosis. Under TEM, in the MIR group some left ventricular tissues were obtained， the nuclear membrane shrank and nuclear chromatin condensed and congregated under the nuclear membrane like speckle，abutting against the nuclear envelope, and pyknosis was found by chance. The mitochondria of some myocardial cells were markedly swollen with some disorganized or disrupted mitochondrial cristae which displayed as an uneven vacuole． Compared with the MIR group, the ultramicrostructure of the SMCH and the DSP groups was improved.Conclusion: High dose SMC can effectively suppress the apoptosis and protects rats against myocardial ischemia.

Protective effect of allicin on homocysteine induced endothelial cell injury

HAO Yuan-yuan,LIU De-shan,WANG Shu-li

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 743-745.

Abstract ( 940 )

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Objective: To explore the reverting effect of allicin on homocysteine induced endothelial cell injury. Methods: Cultural endothelial cells were divided into 5 groups: the homocysteine group, the high dose allicin group, the middle dose allicin group，the low dose allicin group and the control group. Different cultural solutions were added, 12, 24 and 36 hours later, and LDH separated from the upper fluid were determined. The MTT colorimetric method was used to observe the survival of endothelial cells. Results: The low and the middle dose allicin groups had an antagonism to homocysteine in endothelial cell injury. The LDH of the middle and the low allicin groups was significantly lower than that of the homocysteine group(P＜0.05). The antagonism of the high dose allicin group to homocysteine in endothelial cell injury was decreased at 36 hours. There was no significant difference between the high dose allicin group and the homocysteine group (P＞0.05). The result of LDH coincided with that of MTT colorimetric method. Absorptiometry of the middle and the low allicin groups was significantly higher than that of the homocysteine group(P＜0.01). There was no significant difference between the high dose allicin group and the homocysteine group (P＞0.05). Conclusion: The level of homocysteine induced injury in endothelial cells is significantly decreased by low and middle doses of allicin, suggesting allicin may protect and cure homocysteinemia.

Effect of Xingnaojing injection on inflammatory reaction and brain edema and preparations on rats with acute brain ischemia

WU Yu-sheng,ZHANG Wen-gao,ZHENG Guo-ling,ZHANG Jian

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(7): 746-748.

Abstract ( 914 )

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Objective: To explore the protection of Xingnaojing injection(XI) on rats with acute brain ischemia. Methods： Seventy-five rats were randomly divided into three groups( 25 in each). The control and model groups were subjected to 0.9% Nacl(4ml/Kg)and the XI group was subjected to XI (4ml/Kg) through the abdominal cavity, twice a day for 5 days. Then the two sides of the general carotids of the model and XI groups were tied-up to cause acute brain ischemia. After the operations, they were subjected to double dosages. Serum tumor necrosis factor-a(TNF-α), intercellular adhension molecule-1(ICAM1) levels, brain edema and preparations were determined. Results： Serum TNF-α, ICAM-1 levels and brain edema of the model group were higher than those of the control and XI groups(P＜0.01). The injury degree of preparations of the model group was heavier than that of the XI group. There were positive correlations between TNF-α and ICAM-1（P＜0.01）, between TNF-α and brain edema(P＜0.01),and between ICAM1 and brain edema（P＜0.01. Conclusion： XI could inhibit the inflammatory reaction and decrease brain edema, and protect the acute brain ischemia of rats.

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