Table of Content

  

24 September 2007
Volume 45 Issue 9

Articles


Articles

Dynamic evaluation of the right atrial structure and function in atrial fibrillation using the acoustic quantification technique

ZHOU Feng-hua,ZHONG Ming,YANG Gui-rong,WANG Yuan-yuan,ZHANG Wei,LI Li,ZHANG Yun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  865-867. 

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Objective: To evaluate the dynamic changes of the right atrial structure and function in atrial fibrillation using the acoustic quantification (AQ) technique. Methods: Dog models with chronic atrial fibrillation were established by rapid atrial pacing. Right atrial AQ waveforms were recorded before rapid atrial pacing and after pacing for 1, 4 and 8 weeks. Results: Compared with the control group, ESV, EDV and EREV of the atrial fibrillation group were increased, and EF and CV were decreased (P＜0.05) at 4 weeks after pacing. And 8 weeks after pacing, ESV, EDV and EREV were significantly increased (P＜0.001), while EF and PAER significantly decreased (P＜0.001) and CV was also significantly decreased (P＜0.05), and the other parameters had no significant changes in the pacing progress. In the atrial fibrillation group, compared with those before pacing, right atrial AQ parameters had no significant changes at 1 week after pacing (P＞0.05); ESV, EREV and EDV were significantly increased (P＜0.05) and EF was significantly decreased (P＜0.05) at 4 weeks after pacing, while ESV, EREV and EDV were significantly increased (P＜0.001) and EF and PAER were significantly decreased (P＜0.001) at 8 weeks after pacing. Compared with the 1week group, ESV, EREV and EDV were significantly increased (P＜0.01) and EF and PAER were significantly decreased (P＜0.01) at 8 weeks after pacing, and other parameters had no statistical significance. Conclusions: Atrial fibrillation may result in right atrial booster pump and conduit function impairment but no changes of reservoir function. AQ technique may be used as a valuable noninvasive method for evaluating right atrial structure and function in atrial fibrillation.

Signal quality assessment of ECG in the intensive care unit

LI Qiao,YU Meng-sun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  868-872. 

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Objective: To develop an algorithm of signal quality assessment of ECG in the intensive care unit. Methods: The signal quality index (SQI) was obtained by analyzing the morphological and statistical characteristics of each waveform and their relationships. The heart rate was robustly estimated based on SQI. The algorithm was evaluated using more than 6?000 hours of simultaneously acquired ECG from a 437patient subset of the MultiParameter Intelligent Monitoring for Intensive Care II database and adding real ECG noises. Results: The SQI was decreased along with a decrease of the signal noise ratio. The SQI had high-positive correlation with the QRS detective sensitivity and positive predictivity. This method provided an accurate HR estimation even in the presence of high levels of persistent noises and artifacts. Conclusion: The signal quality assessment algorithm could provide an objective estimation of ECG quality.

The targeted therapeutic effect of a lipid-coated microbubble carrying Taxol on C6 glioma in vivo and vitro

GONG Song-feng,LI Xin-gang,LI Gang,WANG Dong-hai,XU Shu-jun,ZHOU Xu-dong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  873-876. 

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Objective: To investigate the therapeutic effect of lipid-coated microbubbles carrying Taxol on rat brain C6 glioma in vitro and vivo. Methods: For the in vitro study, C6 glioma cell lines were treated with Taxol-LCM or LCM. The distribution of LCM and changes of cell morphology and ultra-structure were observed by immunoflurescent microscopy and confocal microscopy. The process of LCMs entering the glioma cells was dynamically observed. For the in vivo study, 12 rats with intra-cranial C6 glioma were divided into 4 groups: groups with LCM-Taxol injection, LCM injection, Taxol injection and the control group. For the in vivo study, each rat was sacrificed to harvest the brain. Oil red O staining was used to determine the therapeutic effect and distributional characters in intracranial C6 glioma. Life spans were also compared. Results: For the in vitro study, Taxol was connected with lipidcoated microbubbles and the compound was endocyted by C6 glioma and had a killing effect. The whole endocyte process was that lipid- coated micro-bubbles first connected with the surface of glioma cells, second were redistributed in the cells, and finally degraded in the cells associated with the acidic component of cytoplasm. Oil red O staining revealed that LCM had aggregated and there was obvious necrosis in the tumor region in the LCM-Taxol injection group. In the LCM injection groups, there was LCM in tumor regions without obvious necrosis. In the Taxol injection group and the control group, there was no nectrosis or LCM. Life span comparison revealed that survival of the LCM-Taxol injection group was much longer than that of the other three groups. Conclusion: Lipid-coated microbubbles may carry Taxol to C6 glioma cells, and the compound may have a killing effect in vitro and in vivo.

Effect of fragile histidine triad gene transfection into the human gastric cancer cell line MGC-803 on Adriamycin

XUE Shu-fang,XU Hong-wei,ZHANG Chun-qing,HAO Jing-hua,QIN Cheng-yong,WANG Wei-bo

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  877-881. 

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Objective: The wild type FHIT gene was transfected into the human gastric cancer cell line MGC-803 in which the FHIT gene had been totally lost so as to investigate its effect on Adriamycin (ADM). Methods: The FHIT gene was transfected into the gastric cancer cell line MGC-803 by liposome. Western bolt assay was employed to determine the expression of FHIT. Cancer cells which were transfected with empty vector pRcCMV and the nontransfected gastric cancer cell line MGC-803 were viewed as a control and a blank respectively. After treatment with Adriamycin, an MTT colormetric assay was applied to determine the inhibition ratio. The apoptosis and cell cycle were analyzed by flow cytometry (FCM). Results: A stable expression of FHIT protein was obtained in transfected MGC803 cells. After treatment with Adriamycin, apoptosis cells were significantly more in FHITtransfected MGC-803 cells than in control cells and blank cells (40.66% VS 13.94%, 15.81%, P＜0.01). Adriamycin-induced apoptosis can be enhanced by wild FHIT expression (P＜0.05). Before and after treatment of Adriamycin, the rates of G0/G1 phase were statistically higher in FHIT-transfected cells than in control cells (74.43% VS 56.30%, 99.27% VS 95.10%, respectively). Conclusion: The FHIT gene might be involved in apoptosis and blocks the cell cycle of gastric cancer cells. Expression of the wild FHIT gene can increase the sensitivity of gastric cancer cell line MGC-803 to Adriamycin.

mRNA expression of lunx in regional lymph nodes of non-small cell lung cancer and its correlation with prognosis

NIU Rui,LIU Bo,SHAO Ming-ju,WANG Wei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  884-885. 

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Objective： To explore the mRNA expression of lunx in regional lymph nodes of non-small cell lung cancer (NSCLC) and its correlation with prognosis. Methods SYBR greenⅠreal-time RT-PCR was used to determine the lunx mRNA level in 153 regional lymph nodes obtained from 75 patients with NSCLC. All patients had been followed up for 3 years. Six factors that may influence the survival rate were analyzed by a Cox regression model. Results： Expressions of lunx in regional lymph nodes had a statistical correlation with age, nutritional index, relapse within 3 years and metastasis(P＜0.01, P＜0.01, and P＜0.05) but had no correlation with gender, tumor types and pathological grades（P＞0.05). There were statistical significances between the survival of NSCLC patients and post-operative adjuvant therapy and expression of lunx (P＜0.05 and P＜0.01). Conclusions： The expression of Lunx in regional lymph nodes closely correlates with the survival of NSCLC patients and it may be a target gene for detection of micrometastasis from NSCLC and a marker for detection in 3 years prognosis. Quantitative real-time RT-PCR is helpful in early diagnosis of micrometastasis of the lymph node in patients with NSCLC.

Diagnostic value and standard of quantitative PCR in rabbits with pulmonary candidiasis albicance

XING Chun-yan,YANG Yan-ping,PANG Long-bin,LI Jun,WANG Xin,ZHOU Jin-hui

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  886-890. 

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Objective: To explore the diagnostic value and standard of quantitative PCR(qPCR) in pulmonary candidiasis albicance in rabbits referring to pathology. Methods: New Zealand white rabbit models of pulmonary candidiasis were developed. Thirty-six rabbits were randomly divided into two groups: the experimental group and the control group. 18 rabbits were enrolled in each group and they were randomly ranked. Rabbits in the experimental group were formed by per cutem intratracheal injection of 0.2ml bacterium fluid containing 1×108 candida albicans(C.albicans). The same volume of normal saline was given in the control group. Rabbits were killed based on a sequence day by day and the pathology of the lungs was observed. Bronchi lavage fluid (BLF) was collected to determine C.albicans by qPCR. Result: If 500 C.albicans/ml was a critical value, the qPCR and pathological results had a significant consistence: sensitivity, specificity, accuracy, positive likelihood ratio and negative likelihood ratio respectively were 71.9%, 92.5%, 83.3%, 9.59 and 0.3(P<0.001). Conclusion: The qPCR is quite consistent with pathology in diagnosis of the disease. The critical value is 500/ml.

Inhibitory effect and mechanism of matrine on the invasive ability of the colon cancer cell line HT-29 in vitro

DING Yin-lu,TANG Si-feng,WANG Jing-yuan,FU Qin-ye,ZHANG Jian-liang,LI Zhan-yuan,LI Zhao-ting

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  891-894. 

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Objective： To investigate the effect of matrine on the invasiveness and expression of heparanas-mRNA in the human colon cancer cell line HT-29. Methods： HT-29 cells were subjected to matrine of different concentrations. Fortyeight hours later, the expression of heparanase-mRNA was determined by semi-quantitative RTPCR, and the level of HPSE protein by western blot. The effect of matrine on HT29 cells was tested by cell Matrigel adhesion assay. The chemotactic migration and the invasive ability of HT-29 cells were determined by Matrigel invasion assay. Results： The expression of hepanase-mRNA and hepanase protein, adhesion, chemotactic migrations and invasiveness of matrine treated HT-29 cells significantly decreased compared with those of the controls(P＜0.01). The inhibitory effect was dose-dependent(P＜0.01). Conclusion： By down-regulating the expression of heparanase-mRNA and hepanase protein, matrine has a significant inhibitory effect on the adhesion and invasiveness of the human colon cancer cell line HT-29 in a dose-dependent manner within a certain range.

Simvastatin promotes the proliferation and mineralization of osteoblasts cultured in vitro

YANG Yun,ZHANG Li-ping,HU Jian-li,ZHANG Zuo-lun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  895-898. 

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Objective: To study the effect of Simvastatin on the proliferation and mineralization of cultured rat calvaria osteoblasts (RCOBs). Methods: RCOBs were cultured in vitro by an enzyme-digestion method and treated with Simvastatin and rhBMP-2 in series gradient of concentration for 24 hours. Then the viability and proliferation of RCOBs were determined by MTT assay. After implantation in a 12well microtiter plate for 7 days, the mineralization nodes were stained by the von Kossa method. Photos were transferred into a black-white style and the mean mineralization area ratio was measured. Results: Simvastatin could not only stimulate the proliferation of the RCOBs in a dosedependent manner， but also increased the mineralization ratio compared with the control group, which mimicked rhBMP-2. Conclusion: Simvastatin can promote the proliferation and mineralization of RCOBs in vitro, which may partially be attributed to the osteogenesis mechanism of statins.

Effects of rh-GH on experimental remodeling of powered periodontal tissues of rats

HUANG Yan,WANG Xu-xia,ZHANG Wen-juan,LIU Chao,ZHANG Jun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  899-902. 

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Objective: To investigate the effect of rh-GH on experimental remodeling of powered periodontal tissues during orthodontic tooth movement in adult rats. Methods: Thirty female 7 week old SPF Wister rats were randomly divided into three groups: the control group, the NS group, the rh-GH group. The orthodontic tooth movement models were established by operation. The distance of tooth movement was measured and the periodontal tissues were histologically studied. Results: The distance of tooth movement of the control group was the shortest, that of the NS group was the longest, and that of the rhGH groual was shorter than that of the NS group and longer than that of the control group in the three groups. All the results had statistical significances. rh-GH significantly improved the periodontal membrane condition and may promote alveolar bone forming and inhibit bone resorption. Conclusion: rh-GH may slow the tooth movement of adult rats and improve the remodeling of powered periodontal tissues.

Microbial inhibitory effect of resinifying dental fissure contents by phenol-formaldehyde resin

JIANG Hao,JIANG Shu-min,ZHU Wen-min,CHENG Ai-hong,LI Ying,JIANG Guang-shui

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  903-905. 

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Objective: To explore the microbial inhibitory effect of phenol-formaldehyde resin in sealing up pits and fissures. Methods: Thirty extracted human molars whose pits and fissures on the occlussal surface had been inoculated with Escherichia coli were randomly divided into three groups: group A was treated with newly confected phenol-formaldehyde resin which resinified the dental fissure contents, group B was sealed Bis-GMA resin in pits and fissures and group C as the control group was only treated by cleaning. Then, after slicing along the fissure, each of the teeth was put in LB medium for culturing to observe the growth of the microbe. Result: The bacteriostatic effect of the phenol-formaldehyde resin group was the best of the three groups. Conclusion: Phenol formaldehyde resin has a strong bacteriostatic effect when used as a pit and fissure sealant. In this respect it is superior to Bis-GMA resin.

Polystyrene sulfonic lanthanum in the treatment of hyperphosphatemia in rats with chronic renal failure

PAN Ming-ming,MIAO Hua,HONG Hong,ZHU Zhu-xian

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  906-909. 

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Objective: To explore the effect of polystyrene sulfonic lanthanum on hyperphosphatemia in chronic renal failure induced by adenine. Methods: Ninety male Waster rats were fed adenine 300mg/kg·d for 15 days and were divided into five groups. The first three groups were fed 1.5g/(kg·d), 0.75g/(kg·d) and 0.375g/(kg·d) polystyrene sulfonic lanthanum, the fourth group was fed calcium carbonate and the fifth group had no drug. Feeding with adenine continued for 10 days in each group and with the corresponding drugs for 22 days. The weight and serum Ca, P, BUN, Scr, RBC, Hb, PLT and HCT were determined. At 22 days, the rats were killed and the kidneys were used!for analysis. Results: The serum phosphorus level was significantly decreased in the group fed polystyrene sulfonic lanthanum of 1.5g/kg per day compared with the normal group ［（2.47±0.38）mmol/l vs （3.46±1.15）mmol/l］（P<0.05）. However, the serum calcium level was significantly increased in the calcium carbonate group compared with the normal group ［（3.48±0.40）mmol/l vs （2.79±0.16）mmol/l］（P<0.01）. Conclusion: High dose polystyrene sulfonic lanthanum could efficiently reduce serum phosphorus levels in rats with chronic renal failure induced by adenine.

Effect of GM-1 on NF-κB in rat retinas after ischemia-reperfusion injury

XIAO Ying,WANG Qi,ZHANG Xiao-fang,JIN Xue-min,ZHANG Xue-song

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  910-913. 

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Objective: To investigate the protective effect of monosialoganglioside GM-1 on rat retinal tissues and the influence of GM-1 on NF-κB after ischemia-reperfusion injury. Methods: Seventy-five healthy adult Wistar rats were randomly divided into 3 groups: the control group(n=5), the NS group(n=35) and the GM-1 group(n=35). The control group received no treatment. Rats in the other 2 groups received complete retinal ischemia by increasing the intraocular pressure for 60 minutes and they were intraperitonially injected to 3 ml·kg-1 NS or 3 ml·kg-1 GM-1 at 12 and 1 hour before and immediately after ischemia. According to the survival time after reperfusion, the experimental 2 groups were subdivided into 7 time points: 0 (only ischemia), 1, 6, 12, 24, 72 and 168 hours after ischemia. Morphological changes and mean thickness of the inner retinal layers (MTIRL) of the retina were determined under light microscopy. Expression of NFκB was determined by the immunohistochemistry method. Results: Rats inner retinas had the lesion process of edema and atrophy after ischemiareperfusion injury.gradually decreased. Pathological changes of retina and activation of NFκB were obviously relieved by GM-1. Conclusion: GM-1 definitely protects against retinal ischemia-reperfusion injury and it is a potential agent for retinal ischemia-reperfusion injury.

The effect of Cuyubanhenxiao on hypertrophic scars in rabbit ears

YAN Hong-shan,LV Run-hua,WANG Ji-chang,ZHAO Jian-ping,LIU Zhen-zhong,DONG Hong,CAI Jing-long

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  914-918. 

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Objective: To explore the effect of Cuyubanhenxiao on hypertrophic scars in rabbit ears. Methods: Twelve adult New Zealand white rabbits were given hypertrophic scars over the ventral surface of the right ears and were randomly divided into the Cuyubanhenxiao group (group A ) and the control group (group B). They were subjected to the drugs once every day for 1 month. The thickness of the scars was determined at the formation and at 4 weeks after treatment. Immunohistochemical (P53 and PCNA) and histomorphology were used to determine the scar tissues at 1 and 4 weeks. Results: The thickness of the scar was significantly different between the two groups (P＜0.05). Expression of p53 and PCNA was significantly different between the 1 week and 4 weeks groups (P＜0.05). Expression of p53 and PCNA was significantly different in group A between the 1 week and 4 weeks groups (P＜0.05), but there was no significant difference in group B between the 1 week and 4 weeks groups(P＞0.05). Conclusions: Cuyubanhenxiao can heal hypertrophic scars of rabbit ears, which may be caused by the decrease of the expression of p53 and PCNA.

Expression of WWOX in tissues of breast cancer and its association with ER and menopausal status

WANG Xiao,CHAO Lan,ZANG Yi-xiu,CHEN Lin-sheng,TIAN Bin,LIU Hui-ping,SUN Jing-zhong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  919-922. 

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Objective： To evaluate the relationship between WWOX expressions at the protein level and the clinic-pathological characteristics of human breast cancer. Method： Expression of WWOX was determined by immunohistochemical staining in a total of 155 tissue micro-arrays including 56 normal breast tissues, 12 ductal carcinoma in situ cases and 87 primary invasive breast carcinoma specimens. Results： A decreased or lost of WWOX expression was observed more frequently in invasive tumors (62.1%) when compared with normal breast tissues (28.6%) (P＜0.01). 82.1% of ER（-）cases and 52.5% of ER（+）cases showed a decrease or lost of WWOX expression(P＜0.05). 22.4% of premenopausal cases were completely negative for WWOX expression versus 51.8% for postmenopausal breast carcinomas (P＜0.05). Furthermore, 23.1% of stage Ⅰ (6/26), 28.6% of stage Ⅱ (10/35), and 46.2% of stage Ⅲ (12/26) tumors showed a negative WWOX protein expression (P＜0.01). Conclusions： Loss of WWOX is a common event in breast cancer. Associations of WWOX expression with ER status, menopausal status and clinical stage of the disease strengthen the hypothesis that WWOX plays a role in carcinogenesis and development of breast cancer in a pathway or pathways associated with sex steroid metabolism.

Expression of WWOX in tissues of breast cancer and its association with ER and menopausal status

YANG Xu-dong,CHEN Jing-han,WANG Xiao-hang,ZHANG Lin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  923-926. 

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Objective： To investigate the expression of E-cadherin and VEGF in esophageal squamous cell carcinoma (ESCC) and the correlation of them with the invasion, metastasis and prognosis of ESCC. Methods： Expressions of E-cadherin and VEGF in 40 specimens of ESCC were determined by immunohistochemistry. Results： The positive rate of E-cadherin in ESCC was 27.5%, lower than that in the control group (P＜0.01). The low expression of Ecadherin correlated with the invasive degree and lymph node metastasis. The positive rate of VEGF in ESCC was 78.0%, higher than that in the control group. The high expression of VEGF correlated with the invasive degree and lymph node metastasis. The expression of E-cadherin was negatively correlated with the expression of VEGF（rs＝-0.4460，P＜0.01）. Conclusions： Expressions of Ecadherin and VEGF correlate with the biological behavior of ESCC, and so it is valuable in evaluating the intensity of metastasis and prognosis of the lymph node.

Alcohol flushing in predicting the efficacy of nitroglycerin on angina pectoris

ZHANG He,CHEN Yu-guo,XU Feng,XUE Li,ZHANG Yun,

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  927-930. 

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Objective: To investigate the value of alcohol flushing to predict the efficacy of nitroglycerin (GTN) on angina pectoris attacks. Methods: Two hundred and thirty-one patients with coronary heart disease, who were confirmed by coronary angiography, were enrolled in this study and they were divided into two groups according to alcohol flushing: the no-flushing group (n=111) and the flushing group (n=120). The genotype of ALDH2 was determined by polymerase chain reaction and direct sequencing. Risk factors of coronary heart disease and blood routine analysis were determined. The efficacy of nitroglycerin on angina pectoris was gained by a questionnaire. Base clinical characteristics and nitroglycerin efficacy between the two groups were compared. Results: The rate of efficacious response to GTN and the ALDH2*1 genotype in the noflushing group was significantly higher than that in the flushing group (88.3% VS 39.2%, P＜0.01; 85.6% VS 41.7%, P＜0.01). The rate of efficacious response to GTN was significantly higher in patients with the ALDH2*1 genotype than in patients with the ALDH2*2 genotype (80.7% VS 32.6%, P＜0.01). Conclusion: Efficacy of GTN can be predicted by alcohol flushing, which may relate to the effect of gene polymorphisms.

Protective effect of Ligustrazine bisphenylmethyl piperazine derivatives on ECV-304 cells injured by hydrogen peroxide

CHENG Xian-chao,LIU Xin-yong,XU Wen-fang,GUO Xiu-li,OU Yang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  931-933. 

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Objective: To evaluate the protective effect of Ligustrazine bisphenylmethyl piperazine derivatives on ECV-304 cells injured by hydrogen peroxide. Methods: ECV-304 cells were injured by hydrogen peroxide and assessed by methyl thiazolyl tetrazolium (MTT) assay. Results: Ligustrazine bisphenylmethyl piperazine derivatives showed protective effects on ECV-304 cells injured by hydrogen peroxide, which were more effective than Ligustrazine. Conclusion: Ligustrazine bisphenylmethyl piperazine derivatives can protect ECV-304 cells against hydrogen peroxide injury.

Relationship between cytoimmunologic status and prognosis of patients with breast cancer

YU Xin-shuang,HAN Jun-qing,WANG Xing-wen,SHENG Wei,WANG Yu

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  934-937. 

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To investigate the cytoimmunologic status of patients with breast cancer and its relationship with prognostic risk factors. Methods： One hundred and ten patients with breast cancer in Shandong Provincial Hospital between 1997 and 2002 were retrospectively analyzed. Clinical features, as well as the alternation of CD⁺₃T, CD⁺₄T, CD⁺₈T, CD⁺₄/CD⁺₈ and LTR and their relationship with mass size, metastasis of lymph nodes and CEA were also analyzed. Results： ①There were statistical differences in the percentage of CD⁺₄T and CD⁺₈T and the ratio of CD⁺₄/CD⁺₈ between groups of different mass sizes (P＜0.05 or P＜0.01). The percentage of CD⁺₄T was decreased and of CD+8T was increased when mass size was small. The ratio of CD⁺₄/CD⁺₈ went down with an increase of mass size. ②Percentages of CD⁺₃T and CD⁺₈T in patients with metastasis of axillary lymph nodes［LN(+)］ were significant higher than those without metastasis of axillary lymph nodes ［LN(-)］ (P＜0.05 or P＜0.01). ratioin patients with metastasis of LN(+) than those without metastasis of LN(-) (P＜0.05 or P＜0.01). The percentage of CD⁺₄T was higher in patients with metastasis of LN(+)than in patients without metastasis of LN(-), but no significant difference was found(P＞0.05). ③The CEA level was significant higher in patients with metastasis of LN(+) than in patients without metastasis of LN(-) (P＜0.05), and no such difference was shown between different mass-size groups(P＞0.05). The increase of CD⁺₄T percentage and the decrease of CD⁺₄/CD⁺₈ ratio were obvious in the CEA(+) group compared with the CEA(-) group(P＜0.01), and CEA was significantly correlated with the CD⁺₄T percentage and the CD⁺₄/CD⁺₈ ratio (P＜0.01). Conclusion： Disorder of the cytoimmunologic situation exists in patients with breast cancer, and it is closely correlated with the highly prognostic factors of mass size and the metastasis situation of axillary lymph nodes.

Durability of the effects after cessation of lamivudine for 47 patients with HBeAg-negative chronic hepatitis B

LI Xiao-ying,WANG Lei,LIU Feng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  938-939. 

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Objective: To investigate the durability of the effects after cessation of lamivudine for HBeAg-negative chronic hepatitis B(CHB) patients. Methods: Forty-seven patients subjecting to lamivudine for no less than 24 months and their HBVDNA-negative duration more than 18 months were enrolled in this study. Hepatic biochemical parameters, HBV-DNA determination, and follow-up were determined after cessation of lamivudine. Results: The cumulative rate of relapse after 3, 6, 12, 24 and 36 months after cessation of lamivudine was 25.5%, 25.5%, 41.8%, 48.3% and 54.8%, respectively. Twenty-three patients relapsed and the latest happened on the 39th month. The cumulative relapse ratio at month 4, 9 and 12 was 52.2%. 65.2% and 82.6%, respectively. The durability of the effects after cessation of lamivudine was related to age at the end of treatment. Conclusion: Durability of the effects after cessation of lamivudine of HBeAg-negative patients is lower than that of HBeAg-positive ones. The younger the patient is, the lower the relapse rate.

The association between genetic polymorphism of superroxide dismutase and chronic obstructive pulmonary diseases

HAN Yi-qun,XUE Yu-wen,LI Chuan-fen,CHE Bo

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  940-943. 

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Objective： To explore the association of genetic polymorphism of superoxide dismutase（SOD） and chronic obstructive pulmonary diseases（COPD）. Methods： 70 patients with COPD （the COPD group）and 70 healthy volunteers (the control group ) were enrolled in this study. Limosis peripheral blood was taken and whole blood corpuscle genomic DNA was extracted. The genetic polymorphism of SOD1-3 genes was determined by polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP). Result： There were no logistic differences between the COPD and the control groups in the genetic polymorphism of SOD1（CuZn-SOD） and SOD2（Mn-SOD）, however, there were logistic differences in the genetic polymorphism of SOD3（EC-SOD）（P＜0.05）. Conclusion： SOD3（EC-SOD）genetic polymorphism (C+760G，Arg213Gly) is related to the development of COPD and it may be the COPDrelated gene.

Expression of HSP70 and Caspase3 in human glioma and its relationship with pathological grades

ZHENG Hong,GENG Ming

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  944-946. 

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Objective: To explore the correlation between Caspase3 and HSP70 expressions and biologic behaviors and pathological grades of human glioma. Methods: Expressions of Caspase3 and HSP70 and their correlation in human glioma were determined by immunohistochemistry(SP). Results: With the increase of tumor clinical grades，the positive rate of HSP70 was increased and the negative rate of Caspase3 was decreased. Expression rates of HSP70 were 25%, 69%, 92% and 100% in grades of Ⅰ-Ⅳ and expression rates of Caspase3 were 87.5%, 61.5%, 38.5% and 31% in grades of Ⅰ-Ⅳ. There was a negative correlation between Caspase3 and HSP70 expression. Conclusion: Expressions of Caspase3 and HSP 70 are correlated with biological behavior and cell differentiation degrees of glioma. They are important markers for the evaluation of prognosis in glioma.

Neurological protective effect of heat-clearing and detoxication treatment for intracerebral hemorrhage in rats

ZHOU Qing-bo,LI Lu-yang,JIA Qing,BI Jian-zhong,SHAO Nian-fang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  947-950. 

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Objective: To investigate the mechanism of neurological protective effect of the Naoningkang granula on brain tissues of rats with intracerebral hemorrhage (ICH). Methods: Rats were randomly divided into five groups: the sham operation group， the ICH model group and the experimental groups (1.2, 2.4 and 4.8?mg·kg^-1 Naoningkang, respectively). CollagenaseⅦ was injected into the caudate nucleus to establish a rat model of ICH. After 2 hours, the Naoningkang granula was given to the experimental groups by gastrogavage, and saline was given to the other 2 groups by gastrogavage. At 3 and 7 days after treatments, brain tissues were taken. HE staining was used to observe the pathological changes. An immunohistochemical method was used to determine the PAR-1 content in brain tissues surrounding hematoma and the dry-wet weight method to assay the water content of brains. Result: The Naoningkang granula significantly improved the pathological changes, inhibited PAR-1 expression and reduced brain edema. Conclusion: PAR-1 expression is up-regulated after ICH in rats, which might play an important role in inducing nerve cell damage and brain edema. The heat-clearing and detoxication method has a significant neurological protective effect on ICH rats probably by inhibiting PAR-1 expression and reducing brain edema.

Pharmacodynamic study of the Yifuqing granula on upper respiratory infection

LI An-yuan,XIE Jan-ying,LI Jie,WANG Peng,XU Shu-lan,ZHAO Lin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  951-954. 

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Objective: To investigate the pharmaco-mechanism of the Yifuqing granula on upper respiratory infection. Methods: The Yifuqing granula was given to rabbit with febrilis caused by the TAB vaccine. The effects of the Yifuqing granula on the ache threshold value of Jimpy mice caused by Hot plate, on auricle inflammatory swelling of Jimpy mice caused by dimethyl benzene, on the bacterium of Jimpy mice in vivo, and on CPE caused by virus were observed. Result: The Yifuqing granula prominently relieved febrilis caused by the TAB vaccine, elevated the pain threshold of Jimpy mice caused by a hot plate, inhibited the swelling of Jimpy mice caused by dimethyl benzene, degraded the fatality of Jimpy mice infected by Staphylococcus aureus and effectively inhibited RSV and Ad3. Conclusion: The Yifuqing granula has an evident effect on relieving fever and pain, dephlogistication, bacteriostasis and antivirus and it is effective in treating upper respiratory infection.

Cervical carotid artery stent placement

GE Hai-tao,WANG Zhi-gang,PAN Shun,DING Xuan,WANG Cheng-wei,QU Chun-cheng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  955-957. 

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Objective: To explore the clinical effects of stent placement for cervical carotid stenosis. Methods: Twenty-six patients with carotid stenosis suffering from repeated transient ischemic attack(TIA) or cerebral infarction were treated with stent placement. All cases were treated with anticoagulants during the peri-operation. Results: Stents were accurately implanted. Stenosis completely disappeared in 19 patients, attenuated by more than 90% in 5 patients and 70% in 2 patients. Cerebral protection devices were used in 10 patients during the operation. No other complications recurred except for cerebral hemorrhages in 1 patient after the operation. No stenosis reoccurred during a follow-up of 3-12 months. The short-term effect was satisfactory. Conclusion: Stent placement for carotid stenosis is safe and effective and its short-term effect is satisfactory.

Effect of UPPP combined with nasal surgery for patients with obstructive sleep apnea-hypopnea syndrome

LEI Da-peng,PAN Xin-liang,LI Xue-zhong,XU Feng-lei,LIU Da-yu,ZHANG Li-qiang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  958-960. 

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Objective: To evaluate the clinical efficiency of uvulopalatopharyngoplasty(UPPP) combined with sub-mucous resection of the nasal septum or inferior turbinate for patients with obstructive sleep apnea-hypopnea syndrome(OSAHS). Methods: All the patients were diagnosed by polysomonography and treated by UPPP. At the same time, sub-mucous resection of the nasal septum was performed on 18 patients, submucous resection of the bilateral turbinate on 20 patients, and adenoidectomy on 2 patients. All of the nasal surgeries were performed by an endoscope. Prophylactic tracheotomy was performed on 5 severe cases. In another 2 cases tracheotomy were performed about 6-7 days before. All the patients were followed up for 1 to 2 years. Results: Symptoms of all the patients were alleviated to some degree: disappeared in 5 patients, reduced in 14 patients, alleviated in 6 patients. No complications occurred. Patients on whom tracheotomies were performed were decannulated in 6-13 days postoperatively. Conclusion: For OSAHS patients, UPPP combined with sub-mucous resection of nasal septum or inferior turbinate could give a better result. For severe OSAHS patients, prophylactic tracheotomy could avoid surgical complications.

Significance of lamellar sectional anatomy of the drainage path of the frontal sinus in operation of the frontal sinus

ZHONG Yan-xia,WANG Qi-rong,TANG Yu-chun,LIU Shu-wei,LIANG Hui,GU Lin-tao,ZHANG Yong-bo

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  961-964. 

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Objective： To provide anatomical evidence for frontal sinus surgery through the agger nasi cell approach and the uncinate process approach by using the lamellar sectional anatomy image. Methods： The computerized cryomilling technique was used to obtain the continuous sectional data of the nasal parts. The frontal sinus′s anatomical boundary and the drainage path were observed. The relationship among the agger nasi cells, the uncinate process and the frontal recess was found. The distance of the center of the agger nasi cell′s anterior wall to the anterior extremity of the middle nasal concha′s root, the thickness of the agger nasi cell′s anterior wall, of the uncinate process′s root, and of the nasolacrimal canal′s medial wall connecting with the uncinate process′s root were determined. Results： The distance of the center of the agger nasi cell′s anterior wall to the anterior extremity of the middle nasal concha′s root was (7.12±0.74) mm. The thickness of the agger nasi cell′s anterior wall was (0.47±0.09)mm, of the uncinate process′s root was (1.27±0.22)mm, and of the nasolacrimal canal′s medial wall connecting with the uncinate process′s root was (1.71±0.08) mm. Conclusion： A precise data of the agger nasi cell′s anterior wall and the uncinate process can be found by the lamellar sectional anatomy image of the frontal sinus.

Neuronavigatio-guided percutaneous radio-frequency thermo-coagulation in treatment of intractable trigeminal neuralgia

WANG Lei,XU Shu-jun,ZHOU Mao-de,JIANG Yu-quan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(9):  965-967. 

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Objective: To investigate the effects of neuronavigatio in radio-frequency thermo-coagulation for trigeminal neuralgia. Methods: Patients with trigeminal neuralgia had continuous and thin-slice CT scans before surgery. The CT data were transported to the neuronavigation system. Being marked and reconstructed in three dimensions, the oval foramen was punctured under neuronavigation and radio-frequency thermo-coagulation was then performed on the patients. Results: The oval foramen was smoothly and accurately punctured under neuronavigation in all. Pain was eliminated in 9 cases and alleviated in 6 cases. No serious complications occurred. Conclusion: Neuronavigatio-guided radio-frequency thermo-coagulation is successful in puncturing the oval foramen and reducing the incidence of complications for trigeminal neuralgia.