Loading...

Table of Content

      
    24 October 2007
    Volume 45 Issue 10
    Articles
    Articles
    Chlamydia trachomatis infects the dendritic cells and affects the function of dendritic cells
    ZHAO Hong-mei,ZHAO Wei-ming,YU Xiu-ping,ZHAO Lei,WANG Hong,ZHENG Yan,LV Hui,LI Jing,LIU Juan,YU Han
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  973-976. 
    Abstract ( 1622 )   PDF (275KB) ( 803 )   Save
    Related Articles | Metrics
    Objective: To explore the survival of Chlamydia muridarum in dendritic cells (DCs) and its effect on function of DCs so as to give a basis for immunopathology caused by Chlamydia trachomatis infection. Methods: Bone marrowderived DCs, which were generated from female BALB/c mice were infected by Chlamydia muridarum, and 24 hours and 72 hours later, fix cells were separately stained with the anti-Chlamydia LPS. Cytokines in the supernatants of the DCs were determined by ELISA; DC and T cells were co-cultured 48 hours later, and T cell proliferation was assayed using CCK-8 kit. Results: Chlamydia muridarum survived in DCs and atypical inclusions developed; After infection with Chlamydia muridarum, DCs secreted a high level of IL-12 and a low level of IL-10; DCs also stimulated the T cell proliferation, which illustrated that infected DCs had the ability to present antigens. Conclusions: The cell model of Chlamydia muridarum infecting DCs has been successfully established. After infected by Chlamydia muridarum, DCs still can present antigens.
    Effect of long-term dietary protein feeding on glucose-stimulated insulin secretion in normal rats
    LI Ming-long,ZHAO Jia-jun,WANG Ming-yan,LIN Xin-ying,LIU Zhan-feng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  977-980. 
    Abstract ( 1743 )   PDF (263KB) ( 830 )   Save
    Related Articles | Metrics
    Objective: To investigate the effects of long-term protein diet with normal total calorie intake on glucose-stimulated insulin secretion in normal rats. Methods: 51 healthy male Wistar rats (8 weeks old) were selected and divided into three groups: the routine feed group (NC, n=16), the high protein diet group (HP, n=19) and the low protein diet group (LP, n=16). The total calorie ingestion of each rat per day was similar and was used for 24 weeks. Body weight, fasting blood glucose (FBG) and fasting plasma insulin (FINS) were monitored during feedings. The insulin secretary function of β cells, especially the acute insulin response(AIR) were determined by the intravenous glucose tolerance test (IVGTT) in 32-week-old rats. Results: 1. Body weight and visceral fat mass: Compared with the NC group, there were no differences of the HP or LP groups. 2. IVGTT: (1) Blood glucose: There were no differences among the three groups. (2) Plasma insulin: 5min- and 10min- plasma insulin levels were markedly higher in the HP group than in the NC group［(6.5±0.8)ng/ml vs (4.9±0.7)ng/ml, P＜0.001; (3.9±0.6)ng/ml vs (3.1±0.5)ng/ml, P＜0.001］, and there were no differences between the LP and NC groups. Conclusion: The long-term high protein ingestion with total calorie may significantly enhance the AIR by IVGTT in rats, while low protein intakes has not significant effect on insulin secretion in rats.
    Long-term epidemiological effect of hemorrhagic fever with renal syndrome vaccine in a large scale population
    ZHANG Gui-qin,XUE Fu-zhong,WANG Jie-zhen,CHENG Yu,SHAO Qi,XU Gui-chun,LI Xue-gang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  981-984. 
    Abstract ( 1323 )   PDF (272KB) ( 616 )   Save
    Related Articles | Metrics
    Objective: To analyze and evaluate the long-term epidemiological effect of the hemorrhagic fever with renal syndrome (HFRS) vaccine in a large scale population in Junan county, Shandong province, from 1995 to 2005. Methods: The experimental epidemiological method was used to establish two groups (the vaccination group and the control group) and contrast the experimental epidemiological indexes, such as the incidence density, the protective rate and the index of effectiveness between the two groups; The cumulative incidence density was calculated and the morbidity level and the protective effect of HFRS vaccine were compared between the two groups at different vaccination times. Results: The incidence density of the vaccination group was significantly lower than that of the control group (χ2=79.9210，P＜0.05), the total protective rate was 92.61%（95% CI：87.09～98.13）and the effectiveness index was 13.54（95% CI: 6.41～28.57）; Based on the vaccination time, the protective rate in the 1st to 11th year was respectively 100.00%, 95.56%, 92.18%, 92.09%, 91.45%, 92.05%, 92.47%, 92.52%, 92.56%, 92.59%, and 92.61%. Conclusion: Using HFRS vaccine to prevent HFRS in a large scale population is effective, and it is unnecessary to consider the boosting within at least 11 years after the scale population.
    Anti-oxidative effect of grape seed extract in the human body
    FENG Dian-juan,XU Gui-fa
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  985-987. 
    Abstract ( 1394 )   PDF (242KB) ( 896 )   Save
    Related Articles | Metrics
    Objective： To study the anti-oxidative effect of grape seed extract (GSE) in human body. Methods: One hundred healthy volunteers aging from 45 to 65 were randomly divided into two groups: the control group and the GSE-treated group based on the SOD, GSH-Px and MDA levels in serum. The GSE capsule was administered to the GSEtreated group 1g/d orally for 3 months, while no drugs were administered to the control group. The SOD, GSH-Px and MDA levels in serum were determined both prior to and after the experiment. Results: The SOD and GSH-Px levels were significantly higher in the GSE-treated group than in the control group (P＜0.05), while the MDA level was significantly lower in the GSE-treated group than in the control group (P＜0.01). Moreover, there were significant differences in the three indexes in the GSE-treated group between prior to and after the experiment (P＜0.01). Conclusion: GSE has a significantly anti-oxidative effect in vivo.
    Slow intra-tumor release of drugs on B16 melonoma in mice
    BU Jie-qiong,YU Bao-fa
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  988-991. 
    Abstract ( 1229 )   PDF (297KB) ( 808 )   Save
    Related Articles | Metrics
    Objective: To explore the effects and mechanism of slow intra-tumor release of drugs on B16 melonoma in mice. Methods: Bearing-tumor mice were randomly divided into four groups: the normal saline group, the Ara-C group, the Ara-C plus releasing drugs group and the the Ara-C plus releasing drugs and DNP group. Four days later, mice were treated again and the gross tumor volume was determined. Then 1 week later, the contents of three fibers were detected in the interstitial substances of the tumor and the infiltrations of T cells were determined in the splenic organs. Results: HE staining results showed that there was a large part of stenosis in the tumor, the fibers in the matrix of tumor were significantly increased and the CD4+T cells were also significantly increased in the two slow-release groups. Conclusion: Slow-release of drugs into the tumor is one of effective managements for directly inhibiting tumor growth and preventing micro-metastasis.
    Effect of Losartan on the permeability of blood-brain barrier and the activity of MMP-9 on focal brain ischemia/reperfusion injury in rats
    QU Si-feng,WANG Jia-ni,WEI Xin-bing,ZHANG Jian-wei,WANG Li-xiang,CHEN Lin,SUN Xia
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  992-995. 
    Abstract ( 1223 )   PDF (277KB) ( 512 )   Save
    Related Articles | Metrics
    Objective： To investigate the effects of AT1 receptor antagonist Losartan on the permeability of blood-brain barrier（BBB）and the activity of matrix metalloproteinase-9 (MMP-9) on cerebral ischemia-reperfusion (IR) in rats. Method： With a model of reversible middle cerebral artery occlusion (MCAO) in rats, the effects of Losartan on the water content of brain, the contents of Evens blue (EB) and the expression of MMP-9 were investigated after 24 hours of reperfusion following two hours of cerebral ischemia. Results： After 24 hours of reperfusion following two hours of cerebral ischemia, the cerebral water content and the contents of EB were increased while MMP-9 was highly expressed. The content of EB in the Losartan group was significantly lower than that in the ischemia/ reperfusion (IR) group (P＜0.05)． Conclusion： Losartan may decrease the activity of MMP-9 and the permeability of bloodbrain barrier in cerebral IR rats．
    Clinical significance of neutrophil myeloperoxidase on peripheral circulation in acute coronary syndrome
    WANG Yuan-yuan,LI Li,ZHOU Feng-hua,MENG Xiao,ZHONG Ming,ZHANG Wei,ZHANG Yun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  996-999. 
    Abstract ( 1451 )   PDF (288KB) ( 581 )   Save
    Related Articles | Metrics
    Objective： To study the changes of peripheral venous blood concentration of myeloperoxidase (MPO) in patients with acute myocardial infarction(AMI), unstable angina pectoris(UAP) and stable angina pectoris(SAP). Methods： 91 patients subjected to coronary angiography, including 19 patients in the AMI group, 30 in the UAP group, 22 in the SAP group and 20 in the control group, were determined the concentrations of MPO and high-sensitivity C-reactive protein (hsCRP) in the femoral vein. The ultra-structure of leukocytes was observed by an transmission electron mirror. Results： The in-gate percentage of MPO in the femoral vein was higher in the UAP group than in the AMI, the SAP and the control groups (P＜0.05, respectively), and there were no differences between the later three groups. The average fluoescent intensity of MPO in the femoral vein was obviously weakened in the AMI group than in the UAP, the SAP and the control groups (P＜0.05, respectively), and still there were no differences between the later three groups. Compared with the control group, concentrations of hsCRP in the UAP and the AMI groups were significantly increased, but no difference existed between the UAP and the AMI groups or between the SAP and the control groups. In the UAP group, patients with type II lesion showed a higher in-gate percentage of MPO (P＜0.05) and a high concentration of hsCRP (P＜0.05) than those with type I lesion. Through an electron mirror, few vacuoles were found in the cytoplasm of the neutrophil in the control group, less particles and more vacuoles in the AMI group, and abundant particles and very few vacuoles in the UAP group. Conclusion: Different changes of MPO between patients with AMI and UAP can not only predict ACS but also reflect the severity of inflammation.
    Effects of α1-adrenoceptor antagonists on cell proliferation and apoptpsis in prostate cancer
    LIU Yi,SUN Ying-hao,WANG Lin-hui,GAO Xu,LI Xiang-tie,ZHANG Ai-min,HAO Jun-wen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1004-1007. 
    Abstract ( 1511 )   PDF (270KB) ( 634 )   Save
    Related Articles | Metrics
    Objective: To investigate the biological action of α1-adrenoceptor antagonists against human androgen-independent prostate cancer cells and to explore its molecular mechanism. Methods: Passage cultures of DU145 cells were exposed to an increasing concentration（0.1，1，10，25，100μmol/L）of doxazosin, terazosin, tamsulosin, or naftopidil for 24，48，72 and 96h, and DU-145 cells not exposing to drugs were served as the control group. The number of viable cells was assessed using an MTT colotmetric. Cells were treated with drugs (25?μmol/L) for 48h, and cell cycles, apoptosis and morphological changes by means of flow cytometry, FACS analysis, and electron microscope technique, respectively. Expressions of apoptosis-related factors and proteins were also determined. Results: After 48h of treatment with doxazosin or terazosin at 25，100μmol/L, the cell viability of DU-145 prostate cancer cells were significantly different from that of the controls（P＜0.01）. Percentages of relative distribution in each phase of the cell cycle were similar to those of the controls. Either drugs resulted in a significant increase in the number of apoptotic cells, and a characteristic appearance of the apoptotic cell morphology was determined by electron microscopy. Immunohistochemical analysis revealed a moderate increased expression of both Fas and TGFβ1, whereas expression of bcl-2 was not significantly different from that of the controls. Tamsulosin and naftopidil had no effect on the prostate cancer cell viability. Conclusions: The quinazoline-derived α1-adrenoceptor antagonists, doxazosin and terazosin, activate apoptosis in prostate cancer cells without interfering with cell cycle progression, which provides a rationale for advancing either drugs as potential antitumor agents in the treatment of advanced prostate cancer.
    A comparison of a rat hind-limb ischemia model established by ligation and embolization
    LIANG Cui-hong,TIAN Hua,XU Yun,LI Gui-bao,TIAN Guang-ping,SONG Tao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1008-1010. 
    Abstract ( 1339 )   PDF (239KB) ( 784 )   Save
    Related Articles | Metrics
    Objective: To explore an effective method for establishing a hindlimb ischemia model in rats. Methods: Wistar rat hindlimb ischemia models were established by three methods: ligation of the femoral artery, ligation of the common iliac artery, embolization of the femoral artery with fine particles of common bletilla tuber (FPCBT). From 1 to 21 days after operation, the ischemia and function had been observed. Results: ① Temporal limp happened in the operated hindlimbs after ligating the femoral artery or the common iliac artery, and no pathological changes were found in the tissue sections; ② At 3 to 21 days after embolizing the femoral artery by FP-CBT, severe ischemia occurred in the operated hindlimbs, such as sustained limping, skin necrosis or ulcers, and muscle fibers necrosis which was recognized by HE staining. Conclusion: Hindlimb ischemia can not be produced by ligating the artery. However, it can be well induced by embolizing the femoral artery with FP-CBT.
    Inhibitory effect of the Yishenhuoxue Capsule on the proliferation of vascular smooth muscle cells induced by HCY in rabbits
    LAI Li-ping,ZHANG Ji-dong,LI Li-zhen,LIU Fen-ye
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1011-1015. 
    Abstract ( 1279 )   PDF (319KB) ( 692 )   Save
    Related Articles | Metrics
    Objective: To explore the effects of the Yishenhuoxue Capsule(YSHXC) on the proliferation of vascular smooth muscle cells (VSMCs) induced by HCY in rabbits. Methods: VSMCs were cultured by tissue explants in vitro and were induced proliferation by HCY. Cells were divided into four groups: the control group, the HCY group, the Yishenhuoxue group and the folic acid group. The proliferation of cells, the expression of PCNA and cell cycles were determined by MTT, immunohistochemistry and flow cytometric technique respectively. Results: After separately incubated with Yishenhuoxue and folic acid, the expression of PCNA was effectively reduced（P<0.01）　and that was especially in the Yishenhuoxue group（P<0.05）. Cell cycles were also homologously changed. Compared with the HCY group, the cell number of the Yishenhuoxue group in the G0/G1 period was increased while in the S period was decreased, which showed that the cell number in the synthesis period of DNA remarkably went down（P<0.01）. Conclusion: Yishenhuoxue can significantly inhibit the proliferation of cells induced by HCY and bring down the level of PCNA, which may be related to the change of cell cycle. Therefore Yishenhuoxue Capsule can delay the process of atheroscleorsis in the earlier period.
    Effect of the Shenqi Jiedu decoction on chronic renal failure: an experimental study
    LI Yun,SI Guo-min,ZHANG Yun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1016-1019. 
    Abstract ( 1232 )   PDF (295KB) ( 719 )   Save
    Related Articles | Metrics
    Objective： To investigate the effects of the Shenqi Jiedu decoction on chronic renal failure(CRF). Methods: Rat models of CRF were established by feeding Adenine. All the rats were randomly divided into five groups: the normal group, the model group, the high dose Shenqi Jiedu decoction group, the low dose Shenqi Jiedu decoction group and the losartan group. After 8 weeks, levels of serum creatinine (SCr), blood urea nitrogen(BUN), plasma albumin(ALB), triglyceride(TG), cholesterol(CHO), Leptin were determined. In addition, the results of pathology were found and the expression of TGF-β1 was determined. Results： Compared with the model group, levels of SCr and BUN in the Shenqi Jiedu decoction groups and the losartan group were decreased while level of ALB was increased (P＜0.05). Pathological results of the Shenqi Jiedu decoction groups and the losartan group were better than those of the model group, and those of the high dose Shenqi Jiedu decoction group were the best. Compared with the model group, expression of TGF-β1 in the Shenqi Jiedu decoction groups and the losartan group were decreased. Conclusions: Shenqi Jiedu decoction can efficiently decrease the SCr, BUN, and Leptin levels and increase the ALB level, reduces the pathological injury and the TGF-β1 expression, and postpones the aggravation of the renal function.
    Different cell factor combinations on human bone marrow AC133+ and CD34+ cells ex vivo
    ZHANG Chun-hua,WEN Ze-qing,ZHU Yong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1020-1024. 
    Abstract ( 1208 )   PDF (260KB) ( 692 )   Save
    Related Articles | Metrics
    Objective: To explore the effects of different cell factor combinations on the human bone marrow CD34+ cells and AC133+ cells ex vivo. Methods: The CD34+ cells and AC133+ cells were gathered by MiniMACS, then the cell factors were divided into 6 groups. Through the ex vivo comparison cultivation under different cell factor combination stimulation, expansion of the CD34+ and AC133+　selection cells was observed and the clone formation of the CD34+ and AC133+ cells was also observed by using Half-solid Methyl cellulose under different cell factor stimulation (in7d, 14d, 21d.). The rate of cell apoptosis was determined. Results: The number the expansion folds and clones of the AC133+ selection cells were higher than those of the CD34+ selection cells under the same condition(P＜0.05).The rate of cell apoptosis from the AC133+ selection cells was lower than that from the CD34+ selection cells(P＜0.05). Conclusion: The AC133+ cells contain more hematopoietic progenitor cells than the CD34+ cells.
    Cell growth and phenolic compounds induced by chitosan in suspension cultures of Marchantia polymorpha L.
    JI Mei,YIN De-ming,LOU Hong-xiang*
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1025-1029. 
    Abstract ( 1421 )   PDF (268KB) ( 519 )   Save
    Related Articles | Metrics
    Objective: To study the role of chitosan elicitor on cell growth and phenolic compound contents of Marchantia polymorpha L. Methods: Cell biomass, chlorophyll, reactive oxygen species (H2O2 and O-2), cell membrane permeability and membrane lipid peroxidation production (MDA) and phenolic secondary metabolism (phenylalanine ammonialyase and phenolic compounds) were investigated in the suspension cultures of Marchantia polymorpha induced by chitosan elicitor. Results: Cell biomass decreased(P<0.05), and chlorophyll content also decreased(P<0.01), and pH in the medium tended to be alkalization(P<0.01). Reactive oxygen species greatly increased (P<0.01) and membrane lipid peroxidation markedly intensified (P<0.01). Meanwhile, the PAL activity and phenolic compounds of secondary metabolism had a significant increase (P<0.01). Conclusion: The ability to de-oxidation decreases and an oxidation increase of Marchantia polymorpha cells induced by chitosan may favor the phenolic compounds biosynthesis.
    Relationship between the expression of HPV/E7,ki-67 and The microvessel dentisy in laryngeal papilloma in adults
    ZHAO Qiu-liang,LI Hai-yan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1030-1033. 
    Abstract ( 1526 )   PDF (248KB) ( 602 )   Save
    Related Articles | Metrics
    Objective: To explore the role of HPV16/E7, the cellular proliferation activity and the tumor angiogenesis in laryngeal papilloma(LP) in adults. Methods: Twenty tissues of LP in adults were selected in the experimental group, and 14 tissues of vocal cord polyp (VCP) and 14 tissues of laryngeal squamous cell carcinoma(LSCC) were enrolled in the control groups. Expression of HPV16/E7 was determined by the polymerase chain reaction(PCR) method, and of Ki-67 and CD34 by the immuneohistochemical SP method. Results: The expression rate of HPV16/E7 in VCP, LP and LSCC was 14.3%, 66.7%, and 85.7%, respectively. The labeling index(LI) of Ki-67 was 6.30±2.03, 13.73±3.42, and 25.55±3.92, respectively. The number of MVD was 3.97±1.38,14.61±1.64, and 25.34±2.63, respectively. The difference was significant (P＜0.01). The level of HPV16/E7, CD34 was positively correlated with that of Ki-67 in the tissues（r=0.854,P＜0.01; r=0.878,P＜0.01）. Conclusion: Ki-67 and MVD highly correlates with the tumor angiogenesis, cellular proliferation activity and pathological types, and they can be served as the malignant indicators for LP in adults.
    Expression and significance of nuclear factor-kappa B ligand and osteoprotegerin in cholesteatoma tissues
    DING Shou-ling,XIA Ming,ZHANG Han-bing,ZHANG Ju-hong,YANG Ying,LIU Fang,XU An-ting
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1034-1037. 
    Abstract ( 1201 )   PDF (258KB) ( 609 )   Save
    Related Articles | Metrics
    Objective: To investigate the expression of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG) in human cholesteatoma tissues. Method: Thirty cholesteatoma samples and twenty normal auditory canal skin specimens were enrolled in this study, and expressions of RANKL and OPG were determined by reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry. Results: The mRNA level of RANKL, but not OPG was significantly increased in the cholesteatoma group compared with the control group（t=4.315,P<0.05；t=0.578，P>0.05）. Meanwhile, the ratio of OPG/RANKL was increased (t=6.127；P<0.05). Expression of the cytokine RANKL was increased in infiltrated lymphocytes in the cholesteatoma tissues compared with the normal external meatal skins（t=7.758，P<0.05）, however, expression of OPG was not significantly changed in the cholesteatoma tissues compared with the normal external meatal skins（t=0.623；P>0.05）. Ratio of OPG/RANKL was decreased in the cholesteatoma tissues compared with the normal external meatal skins（t=8.183；P<0.05）. Conclusion: This study reveals that the expression level of RANKL is markedly increased in the perimatrix of cholesteatoma and is closely related to bone resorption induced by cholesteatoma. The decreased OPG/ RANKL is probable a risk factor of the bone resorption induced by cholesteatoma.
    18F-FDG PET-CT for lymph node staging in non-small cell lung cancer
    ZHANG Cheng-qi,WANG Guang-li,SONG Ji-qing,YAO Shu-zhan,LIU Qing-wei,CHE Jing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1038-1041. 
    Abstract ( 1419 )   PDF (284KB) ( 599 )   Save
    Related Articles | Metrics
    Objective： To investigate the clinical value of 18F-FDG PET-CT for non-small cell lung cancer in lymph node staging. Methods：18F-FDG PET-CT (Discovery LS PET-CT in Department of Radiology of Shandong Provincial Hospital) images of 79 patients who had undergone surgeries for histopathologically proved non-small cell lung cancers without distant metastases were analyzed. PET-CT results were compared with the CT data. Findings of both modalities were compared with pathological results and were statistically analyzed. Results： There were 268 lymph node metastases in 79 patients. The sensibility, specificity and accuracy of PET-CT and CT for detecting lymphoid node metastasis were 82.84%, 96.70% and 89.83% and 58.58%, 72.89% and 65.80%, respectively. The former was obviously better than the latter. Overall tumor stage was correctly classified as N stage with CT in 58 patients and with PET-CT in 73 patients. Differences in the accuracy of lymphoid node staging between PET-CT and CT were significant (χ2=10.05, P＜0.01). Conclusion： PET-CT is a very effective imaging modality for lymph node staging of nonsmall cell lung cancer.
    64-slices spiral CT angiography and selective coronary angiography for the coronary artery:a comparative study
    WANG Xi-ming,CHENG Zhao-ping,WU Le-bin,WU Da-wei,CHAO　Bao-ting,LIU Cheng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1042-1045. 
    Abstract ( 1372 )   PDF (272KB) ( 706 )   Save
    Related Articles | Metrics
    Objective： To study the clinical effect of 64-slices spiral CT in coronary artery. Methods： 64-slices spiral CT angiography was performed on 256 patients. Simultaneously, 66 patients of them were also subjected to selective coronary artery angiography. Results： Coronary artery branches and part sub-branches were clearly displayed in 249 patients. Stenosis was found in 201 coronary artery branches of 138 patients. In 62 cases of conventional angiography, all main branches and parts of sub-branches of coronary artery were clearly displayed. All patients demonstrated coronary artery stenosis. The diagnosis accuracy of 64-slices spiral CT was 93.94%. Conclusion： As a minimally invasive examination, 64-slices spiral CT is a valuable method to detect and diagnose coronary artery diseases.
    Aberrant methylation of the RASSF1A gene in tissues and serums of ovarian cancer patients and its clinical significance
    LI Qi-rong,LIU Pei-shu,ZHANG Yan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1046-1049. 
    Abstract ( 1483 )   PDF (260KB) ( 705 )   Save
    Related Articles | Metrics
    Objective: To explore the correlation between hypermethylation of RASSF1A gene and ovarian cancer. Method: Sixty-seven tissue samples and their corresponding serum samples from ovarian cancer patients were collected for hypermethylation measurement by semi-nested methylation-specific PCR(MSP). Results: Hypermethylation of the RASSF1A was presented in 28.4%(19/67) of the cancer serum samples and 40.3%(27/67) of the cancer tissues. None of benign ovarian disease tissues and serums was methylated. Hypermethylation of the RASSF1A was not found in serum samples from healthy volunteers. There was statistical association between hypermethylation of RASSF1A in serums and in cancer tissues (P＜0.01). Hypermethylation of the RASSF1A in serum and the corresponding cancer tissues were not significantly correlated with the clinical stage, histologic cell types, degree of cell differentiation of the tumor(P＞0.05); but it was strongly correlated with the lymph node metastasis(P＜0.01). Conclusion: Hypermethylation of the RASSF1A may be a useful marker in the auxiliary diagnosis of ovarian cancer .
    Anti-calcification function property of allogenic arteries cross-linked by dye0mediated photooxidation
    GU Zhi-tao,LIU Lu-qi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1050-1053. 
    Abstract ( 1257 )   PDF (297KB) ( 560 )   Save
    Related Articles | Metrics
    Objective: To study the anti-calcification function property of rabbit thoracic aorta cross-linked by dye-mediated photo-oxidation. Methods: Rabbits whose allogenic thoracic aorta was cross-linked by dye-mediated photo-oxidation (DMP, n=6) were in the DMP group, rabbits whose allogenic thoracic aorta was cross-linked by glutaraldehyde (GA, n=6) were in the GA group, and rabbits whose homogenic abdominal aorta was orthotropically transplanted (OT, n=6) were in the OT group. Then the donor arteries were transplanted in the position of abdominal arteries of rabbits. After operations, animals were fed for 1 month, and then the grafted artery was taken off for observation by HE, SEM and tissue calcium content which was analyzed by flame atomic absorption spectrophotometer. Results: Results of calcium content in the DMP group, the GA group and the OT group was 3.58±0.22μg/mg, 22.5±0.53μg/mg and 2.99±0.33μg/mg, respectively. There was no statistical difference between the DMP group and the OT group (P＞0.05). However, it revealed a significant difference between the DMP group and the GA group (P＜0.01). The calcium content in the DMP group was much lower than that in the GA group. Conclusion: The artery cross-linked by dye-mediated photo-oxidation treatment appears to have less calcification in rabbit allogenic transplantation model.
    Therapeutic effects of Huayulifei on expression of SMAD7 and TGF-β in rats with bleomycin-induced pulmonary fibrosis
    JIAO Fang-fang,LIU Shi-qing,LI Fei,LI Chang-sheng,WANG Qin,SUN Qing,LU Wei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1054-1058. 
    Abstract ( 1287 )   PDF (320KB) ( 564 )   Save
    Related Articles | Metrics
    Objective: To evaluate the therapeutic effects and mechanism of Huayulifei on blemycin-induced pulmonary interstitial fibrosis in rats. Methods: Rats were randomly divided into four groups: the control group, the model group, the Huayulifei group and the hydrocortisone group. Pulmonary fibrosis was induced by blemycin(5mg/kg) in the Huayulifei and hydrocortisone groups, while 0.9% saline was given to the control group. At the second day, Huayulifei（oral, 13410mg/kg）was given to the Huayulifei group, intraperitoneal Hydrocortisone sodium succinate（25mg/kg）to the Hydrocortisone group, and oral saline to the control and model groups. Rats were killed at14 and 28 days after bleomycin. The lung was isolated and calculate the lung coefficients. Hematoxylin and eosin-staining, and expressions of TGF-β and Smad7 proteins were determined by the immunohistochemical technique. Results: Forty-four rats were involed in the analysis. ①:The airsacculitis and fibrosis of lungs were fairly severe and much deposition of the collagen fibers was found in the model group.There was little inflammatory cells, the alveoli structure was nearly normal, and most of the airsacculitis and fibrosis of lungs was mild in the Huayulifei group. The alleviation of airsacculitis and fibrosis of lung were not signficantly decreased in the hydrocortisone group. ② Expression of TGF-β protein was significantly lighter in the Huayulifei group than in the model and hydrocortisone groups; expression of Smad7 protein was significantly higher in the Huayulifei group than in the model and hydrocortisone groups. Conclusion: Huayulifei can ameliorate the blemycin-induced pulmonary fibrosis in rats probobly by improving the expression of Smad7 in lung tissues and decreasing the expression of TGF-β protein by feedback inhibition.
    Relationship between expression of cyclin E and nerve infiltration and prognosis in cholangiocarcinoma
    BI Xiao-feng,DU Qi-hang,MU Qing-ling,ZHANG Bao-ning
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1059-1062. 
    Abstract ( 1173 )   PDF (270KB) ( 574 )   Save
    Related Articles | Metrics
    Objective: To explore the expression of cyclin E in cholangiocarcinoma and investigate the relevance of cyclin E and several clinical factors with infiltration of nerve and prognosis. Methods: The S-P immunohistochemical staining method was used to determine the expression of cyclin E in 35 cases of cholangiocarcinoma tissues, 25 cases of normal bile duct tissues beside tumor and 11 cases of bile duct′s benign disease. Results: The positive expression rate of cyclin E in cholangiocarcinoma was higher than that in infiltration of surroundings（P＜0.01） and tissues of bile duct′s benign diseases（P＞0.05）. The positive expression of cyclin E was related to infiltration of nerve（P＜0.01）, but was not related to the size of tumor, pathologyic stage, infiltration and clinical TNM stage（P＞0.05）. The median survival time was significantly longer in patients negative for the cyclin E expression than that in those positive for the expression（P＜0.01）. Conclusions: The positive expression of cyclin E is related to infiltration of nerve. cyclin E may be one of markers to evaluate the prognosis of cholangiocarcinoma.
    Relativity between pulse wave velocity and coronary atherosclerosis
    LI Pei,LIANG Jiang-jiu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1063-1067. 
    Abstract ( 1302 )   PDF (277KB) ( 578 )   Save
    Related Articles | Metrics
    Objective: To explore the correlation between pulse wave velocity(PWV) and atherosclerosis. Methods: Twenty-four male New Zealand rabbits were randomly divided into three groups with 8 in each: the control group, the model group and the atorvastatin group. They were respectively fed with standard diet, standard diet containing 3% cholesterol and standard diet containing 2.5mg/(kg·d) atorvastatin plus 3% cholesterol diet. After 12 weeks, aortas were removed under deep anesthetization. The degree of the atherosclerosis stenosis of coronary artery branches in the heart walls and the PWV were determined. Masson staining was performed to observe the collagen deposition in atherosclerostic plaques. Level of COX2 protein and NFκB protein was determined by SABC immunohistochemistry. Results: Compared with the control group, degree of the atherosclerosis stenosis of coronary artery branches and the PWV were significantly increased in the model group (P＜0.05). COX-2 protein expression and collagen accumulation in plaques of atherosclerosis rabbits were significantly increased compared with the control group, and were significantly decreased by atorvastatin (both P＜0. 05). The degree of atherosclerosis stenosis was related to the PWV, and the level of COX-2 protein was related to NF-κB protein in atherosclerostic plaques(r=79 and 0.81, both P＜0.05). Conclusion: PWV is related to atherosclerosis stenosis and can reflect the degree of atherosclerosis stenosis of coronary artery branches in the heart walls.
    Effect of psychological treatment for patients with irritable bowel syndrome
    SONG Dong-ling,WANG Meng-xin,ZENG Juan,LI Yan-qing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1068-1071. 
    Abstract ( 1414 )   PDF (257KB) ( 737 )   Save
    Related Articles | Metrics
    Objective: To observe the therapeutic effect of psychological treatment on patients with irritable bowel syndrome. Methods: Fiftyseven patients were enrolled in this study and were randomly divided into two groups: the experimental group （EG）and the control group（CG）. Both groups were treated for 12 weeks. Results: After treatments, global symptoms and all the other symptoms except for abdominal distension, especially abdominal pain or discomfort were released in the EG (5.10±0.74 vs 3.10±1.10, P＜0.05). The overall average scores of Symptom Cheecklist90, Hamilton Anxiety Scale and Hamilton Depression, also especially the scores of depression (2.45±0.03 vs 1.34±0.04, P＜0.01) and anxiety (2.56±0.07 vs 1.46±0.07, P＜0.01) were significantly decreased in the EG. Conclusion： Psychological treatment is an effective and safe treatment for patients with irritable bowel syndrome.
    Jaw thrust device (JTD) on obesity patients for maintaining the airway patency during general anesthesia induction
    SHU Ya,QI Feng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(10):  1072-1074. 
    Abstract ( 1676 )   PDF (247KB) ( 736 )   Save
    Related Articles | Metrics
    Objective： To explore the efficacy and safety of jaw thrust device(JTD) in keeping the airway patency on obesity patients during general anesthesia induction. Methods： Anesthetic induction with midazolam, propofol, fentanyl and atracurium was performed on 25 patients, whose BMI≥30, scheduled for selective surgery. JTD was connected to the anesthetic machine via anesthetic face mask to control respiration. After the muscle thoroughly relaxed, airway peak pressure (cmH2O) and airway patency were clinically assessed with Stridor score, breathing sounds detected by auscultation over the trachea were recorded twice at the head neutral posture， head back tilt，jaw thrust maneuver and jaw thrust device positions randomly. Then an endotracheal intubation and control respiration were given at once. Results： The airway peak pressure was higher at the head neutral and head back tilt posture than that at jaw thrust maneuver and JTD posture （F=356.2，P＜0.001）. The Stridor median score was significant higher at the head neutral and head back tilt posture than that at jaw thrust maneuver and JTD posture （χ2=587.6，P＜0.001）. Values of airway peak pressure and Stridor score showed no significant difference between jaw thrust maneuver and JTD posture. Conclusion： JTD is safe and stable for maintaining the airway patency during anesthetic induction for obesity patients and can effectively substitute the jaw thrust maneuver. JTD also provides an easy airway management on difficult airway patients for the anesthesiologists.
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd