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Table of Content

      
    24 December 2007
    Volume 45 Issue 12
    Articles
    Articles
    Effect of sesquiterpenoids on proliferation activity of prostate cancer cells
    WANG Xiao-ling,KONG Feng,XU Ai-hui,JI Kai,CAI Jie,REN Kai,YUAN Hui-qing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1189-1192. 
    Abstract ( 1260 )   PDF (253KB) ( 694 )   Save
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    Objective To investigate the effects of sesquiterpenoids on proliferation of prostate cancer cells. MethodsThe growth of prostate cancer cells treated with sesquiterpenoids from myrrh was determined by MTT. The cell cycle was analyzed by flow cytometry. Also the expressions of p21WAF/CIP1(p21)（p21） and cyclinD at mRNA levels were determined by reverse transcription PCR (RT-PCR). ResultsThe two sesquiterpenoids significantly inhibited the proliferation of prostate cancer cells and caused a G0/G1 arrest, also it induced the expression of p21 and decreased the expression of cyclinD. ConclusionOverexpression of p21 and decrease of cyclinD might contribute to the two sesquiterpenoidmediated inhibitory effects on prostate cancer cell growth.
    Effects of caspase-3 activation and cytochrome c release in cerebral ischemia reperfusion injury
    ZHANG Wen-hua,ZHOU Lu,XU Shu-jun,CHENG Teng,JIA De-ze,ZHOU Mao-de,WU Cheng-yuan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1193-1196. 
    Abstract ( 1428 )   PDF (279KB) ( 696 )   Save
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    ObjectiveTo evaluate the effects of caspase-3 activation and cytochrome c release in cerebral ischemia reperfusion induced neuronal cell death. MethodsFocal cerebral ischemia C57L mice model was established by the middle cerebral occlusion (MACo) method. Caspase-3 activity was determined by an enzyme activity assay at different time points after ischemia reperfusion. Caspase-3 activation and cytochrome c release were determined by immunohistology staining and Western blot. Results①Caspase-3 activation immediately occurred at 3h after ischemia reperfusion and was gradually increased till to the peak at 12h to 24h. ②Caspase-3 was significantly activated at 6h after completion of ischemia reperfusion and it was increased by 7.6 times in comparison with the sham control (P＜0.01). ③Cytochrome c release occurred at 6h after completion of ischemia reperfusion and it was increased by 8.5 times in comparison with the sham control (P＜0.01). Conclusions Caspase-3 activation and cytochrome c release play a critical role in neuronal cell death induced by cerebral ischemeia reperfusion. It provides insights into the mechanism of events involved in ischmeia reperfusion injury.
    Association analysis of single nucleotide polymorphism in STAT4 gene promoter region with asthma in Chinese Han population from Shandong Province
    ZHANG Wen-jing,LIU Qi-ji,GAO Gui-min,LI Huai-chen,WEI Chun-hua,GONG Yao-qin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1195-1200. 
    Abstract ( 1186 )   PDF (269KB) ( 717 )   Save
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    ObjectiveTo explore the association of a single nucleotide polymorphism (SNP)， rs16833431 A＞G， in the promoter region of signal transducer and activator of transcription 4 (STAT4) with asthma in Chinese Han population from Shandong Province. MethodsSNP rs16833431 A＞G was genotyped by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) in 220 asthmatic subjects and 233 controls from Shandong Province. Chi-square test was used to determine the association of this SNP with asthma. ResultsThe frequencies of genotypes AA, AG and GG at SNP rs16833431 A＞G were 0.536, 0.377 and 0.086 in asthma patients, while 0.515, 0.399 and 0.086 in healthy controls. ConclusionNo significant difference is observed between STAT4 promoter polymorphism and asthma in Chinese Han population from Shandong Province.
    Effects of 2R, 4R-APDC on learning and memory of rats after epilepsy
    YAO Hong,FENG Ya-bo,ZHAO Xiu-he,CHI Ling-yi,CHI Zhao-fu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1201-1204. 
    Abstract ( 1173 )   PDF (293KB) ( 628 )   Save
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    ObjectiveTo study the effects of 2R, 4R-APDC, a selective group Ⅱmetabotropic glutamate receptor (II mGluR) agonist, on learning and memory of rats after epilepsy. MethodsSD rats were used to reproduce lithium and pilocarpine epileptic model, then they were intracerebroventricularly(icv) given 2R, 4R-APDC or normal saline. 24 hours later, rats′ behaviors were determined by the elevated plus maze test and the Morris water maze test. ResultAfter the epileptic rats having been intra-cerebroventricularly given 2R and 4R-APDC, time spent in the open arms was shortened and number of entries to the open arms was reduced(P<0.05), the mean escape latency was shortened(P<0.01), and the ability of spatial memory and the efficiency of their search strategy were improved (P<0.05). Conclusion2R, 4R-APDC by icv can improve the epileptic rats′ ability of learning and memory.
    Effects of Tongxinluo and Simvastatin on vulnerable plaques in rabbits: a comparison study
    LU Xiao-ting,ZHANG Lei,LIU Yan,LIU Yun-fang,YAN Fang-fang,ZHAO Yu-xia
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1205-1208. 
    Abstract ( 1364 )   PDF (293KB) ( 742 )   Save
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    Objective To compare the stabilizing effects of Tongxinluo and simvastatin on vulnerable plaques in rabbits. Methods30 rabbits were subjected to ballooninduced abdominal aortic wall injuries and then were fed with a dietary of 1% cholesterol. After 10 weeks induction, they were randomly divided into three groups: the regression group, the Tongxinluo group and the simvastatin group. At the end of week 24, recombinantp53 adenoviruses were locally delivered to the atherosclerotic plaques and plaque rupture was triggered by the intraarterial viper venom and histamine. The serum lipids, the high frequency duplex, the acoustic densitometry and the pathologic staining were all determined. ResultsCompared with the regression group, TC, LDL-C（P＜0.01）and IMT （P＜0.050.01）in the Tongxinluo group and the simvastatin group were significantly decreased while the AIIc %（P＜0.050.01）was significantly increased. The TC level in the simvastatin group was lower than that in the Tongxinluo group（P＜0.01）. Conclusion Both Tongxinluo and simvastatin can increase the stability of atherosclerosis in rabbits with vulnerable plaques.
    Expression and significance of Connexin 43 on neural epithelium during neural tube defects
    MA Yong-zhen,WU Yu-ling
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1209-1212. 
    Abstract ( 1233 )   PDF (303KB) ( 805 )   Save
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    Objective To investigate the expression of Connexin 43(Cx43) in neural tube and to explore its role in the pathogenesis of neural tube defects(NTDs). MethodsNTDs models were established in mouse embryo by overdose ratinoic acid(RA). The neural tube developmental condition and the expressed position and intension of Cx43 between the control group and the experimental group were determined by SABC immunohistochemistry method and analyzed by image processing system. ResultsIn the control group the neural tube well grew and closed up at the right moment. In the experimental group the neural tube was poorly developed and unclosed. Brain vesicle could not grow well. The Cx43 positive immunostaining located not only on the membranes but also in the cytoplasms of neural epithelium, and the Cx43 positive rate in the experimental group was higher than that in the control group（P＜0.05）. The expression of Cx43 had a temporal variability. Conclusion The hyperexpression of Cx43 may play an important role in the development of TNDs induced by RA.
    Combination of Selenium and vitC inhibits Psoriasis
    HU Wei,MA Sai,GUO Dong-mei,JI Jing,YANG Xiao-qian,CUI Xi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1213-1215. 
    Abstract ( 1491 )   PDF (277KB) ( 804 )   Save
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    Objective To investigate the effects of Na2SeO3 combined with Vit C on the activities of SOD, GSH-PX， the content of MDA in the liver and kidney of the mice, and mitotic division of vaginal epitheliums. Methods Female Kunming mice were used to establish the mitotic division model of vaginal epitheliums. They were randomly divided into eight groups: the negative control, the positive control, the low, middle and high dosage of Na2SeO3, and the low, middle and high dosage of Na2SeO3 and Vit C. The corresponding reagent was administered for twelve consecutive days.　 One hour after last administration, colchicines was injected into the abdomen of the mice, and then the mice were sacrificed by taking the cervical vertebrae off. Kidney and liver tissues were made into homogenate for determining the enzymatic activity. Simultaneously, the vaginal mitotic division was observed under a light microscope. ResultsActivities of SOD and GSH-PX in livers and activities of GSH-PX in kidneys in each selenium replenished group and methotrexate group were significantly higher than in the negative control group(P＜0.05), while the MDA content difference was not statistically significant among them. Both Na2SeO3 and Na2SeO3-Vit C could obviously inhibit the mitoses of vaginal epithelial cells(P＜0.05), but no statistically significance was found between them and the positive control group. Conclusion Na2SeO3 could raise activities of GSHPX in liver and kidney, promote the effect of antioxidizing system and thus effectively inhibits the mitotic division of vaginal epitheliums. Na2SeO3 and Vit C may not act in coordination.
    Expression and significance of TRAIL and its receptors DcR1 and DR4 in cervical cancer tissues
    ZHANG Wen-feng,ZHU Lin,ZHANG Ping
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1216-1219. 
    Abstract ( 1171 )   PDF (264KB) ( 555 )   Save
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    Objective To determine the expression of TNF-related apoptosis inducing ligand (TRAIL) and its receptor DcR1, DR4 in cervical cancer and normal cervical tissues. MethodEnvision immunohistochemical method was used to determine the expressions of TRAIL, DcR1 and DR4 in tissues of 43 cases of cervical cancer and 30 cases of normal cervical tissues. ResultsThe expressions of TRAIL, DcR1 and DR4 were found in the cell membrane and cytoplasm and the expressions of TRAIL and DcR1 in cervical carcinoma tissues were significantly lower than those in the normal cervical tissues, while the expression of DR4 was higher in the cervical carcinoma tissues than in the normal cervical tissues(P＜0.05). The positive expression of TRAIL, DcR1, and DR4 in cervical carcinoma tissues was not related to patient's age, stage, lymphoid node diversion. Conclusion TRAIL may be one of the markers of poor differentiation of carcinoma cells. Cervical carcinoma cell apoptosis might be easily induced by TRAIL because of the high expression of DR4 and the low expression of DcR1.
    Ursolic acid induces apoptosis in endometrial carcinoma HHUA cells
    ZHANG Xian,YANG Xing-sheng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1220-1223. 
    Abstract ( 1107 )   PDF (284KB) ( 476 )   Save
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    Objective To study the effects of ursolic acid (UA) on growth of the endometrial cancer cell line HHUA. MethodsThe HHUA cells were treated with ursolic acid at different concentrations. The proliferation inhibition was determined by MTT assay. Morphological examination and flow cytometry were used to determine the apoptosis. RTPCR and immunohistochemical method were used to determine the expressions of apoptosis related genes caspase-3 and bcl-2. The semiquantification of protein expression was analyzed by pathological imageanalysis. ResultsUA strongly inhibited the proliferation of HHUA cells. Apoptosis was induced by UA treatment. The morphology of HHUA showed changes such as chromatin aggregation, nuclear and cytoplasmic condensation and apoptotic body appearance. UA caused significant G0/G1 phases arrest with a concomitant decrease of cell population in S and G2/M phases. Apoptotic cells were found by flow cytometry. The maximal early apoptotic rate was (29.1±2.43)% when exposed to 40umol/L for 72?h. The expression of caspase-3 gene was enhanced while the expression of antiapoptotic gene bcl-2 was decreased. All these effects were in a dose- and time-dependent manner. Conclusion UA induces the apoptotic processes in the endometrial cancer HHUA cell line by involving the caspase-3 pathway and bcl-2 family proteins.
    Construction and expression of the vector of CD137L under the control of hTERT promoter in human ovarian tumor cell lines
    ZHANG Xi,CUI Bao-xia,MA Dao-xin,KONG Bei-hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1224-1228. 
    Abstract ( 1023 )   PDF (283KB) ( 588 )   Save
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    ObjectiveTo construct an expression vector of CD137L under the control of hTERT promoter and to investigate the expression of this gene in human ovarian tumor cell lines and the possibility of the targeted gene therapy of CD137L. MethodsThe CD137L gene was cloned from the K562 cell lines and was inserted into the pcDNA3 vector to form a new plasmid named pcDNA3-hCD137L. The plasmid was digested by Hind Ⅲ and Xba Ⅰ, then the CD137L fragment was inserted into the pBTdel279 plasmid to get a new plasmid pBTdel-279-hCD137L, in which the expression of CD137L was under the control of hTERT promoter. The new plasmid was identified by digestion of restriction enzyme and sequencing. pcDNA3-hCD137L and pBTdel-279-hCD137L plasmids were transfected into human ovarian tumor cell line OVCR3 and human embryonic lung fibroblast cell line HELF. The expression of hCD137L was determined by RT-PCR and flow cytometry. ResultsThe plasmids were properly constructed. Both OVCR3 and HELF cell lines can express hCD137L after transfection with pcDNA3-hCD137L plasmids, but only OVCR3 cell line can expression hCD137L gene after transfection with pBTdel-279-hCD137L plasmids. The results of flow cytometry showed that the transfection efficiency of pBTdel-279-hCD137L was much lower than that of the pcDNA3-hCD137L plasmid. ConclusionsThe expression vector of CD137L under the control of hTERT promoter is successfully constructed and the expression of hCD137L gene can be targeted in tumor cells by the hTERT promoter. But the activity of hTERT promoter is much lower than that of the CMV promoter. How to increase the activities of the hTERT promoter needs further investigation.
    Relationship between proinflammatory cytokine gene polymorphisms and diseases of HBV infection
    XING Pei-xianga,ZOU Ming-jina,XING Quan-taib,WANG Hong-chuna,WANG Yun-shan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1229-1233. 
    Abstract ( 1225 )   PDF (297KB) ( 663 )   Save
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    ObjectiveTo investigate relationships between the single nucleotide polymorphisms(SNPs) of tumor necrosis factor-α(TNFα) or interleukin-6(IL-6)promoter genes and susceptibility to patients with chronic hepatitis B (HB)in Jinan area. MethodsThe SNPs of the TNF-α(-238G/A and -308G/A) and IL-6(-597G/A, -174G/C and -572G/C) were determined by gene chips in 150 patients infected with hepatitis B virus(HBV) and 100 unrelated healthy controls of selflimited HBV infection. ResultFrequencies of TNF-α-308GG genotype and G allele in patients with HBV infection were significantly higher than those in healthy controls（P＜0.01，OR=6.71， and P＜0.01，OR=2.22， respectively）. TNF-308AA genotype had a cooperative effect on the antiviral therapy （P＜0.05，OR=2.91）. No association was found between any of the other polymorphisms and patients with HBV infection. Conclusion TNF-α-308GG genotype and G allele are significantly related to patients with HBV infection, and TNF-308AA genotype is associated with the response to antiviral therapy.
    Inhibitory effect of c-FLIP-ASODN on transplantation tumor of esophagus cancer cell line EC109 in nude mice: an experimental study
    YANG Kui-zhong,SUN Xue-fei,XIANG Ji-shun,DU Qing-cong,HUANG Feng-chang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1234-1238. 
    Abstract ( 1178 )   PDF (310KB) ( 809 )   Save
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    ObjectiveTo explore the inhibitory effect of cellular Fas-associated death domainlike IL-1 beta converting enzyme inhibitory protein (c-FLIP) antisense oligodeoxynucleotides (ASODN) on transplantation tumor of esophagus cancer cell line EC109 in nude mice. MethodsThe nude mice bearing EC109 cancer cells were established and divided into the control group, the liposome group, the sense oligonucleotide(SODN)group and the ASODN group．The transplantation tumor growth curve was drawn, and the inhibition ratio was calculated according to the tumor weight. Immunohistochemistry SP method was used to determine the expression of c-FLIP and Ki-67 antigen of the transplantation tumor. RTPCR was used to determine the expression of c-FLIP-mRNA. Terminal deoxynucleotidyl transferase mediated UTP nick end labeling (TUNEL) was used to determine the apoptosis of the transplantation tumor. ResultsThe transplantation tumor of the ASODN group slowly grew after taking drugs. The tumor volume and the tumor weight of the ASODN group were decreased compared with those of the other control groups. The tumor inhibitory rate was 68.19％. The expressions of c-FLIP mRNA and protein，and the Ki-67 antigen of the ASODN group were lower than those of other control groups. The apoptosis of the transplantation tumor of the ASODN group was higher than that of the other control groups. There were statistically significant differences between the ASODN group and the other groups(P<0.05), but no statistically significant differences were found among the control group, the liposome group and the SODN group(P>0.05). Conclusion c-FLIP ASODN has an anticancer activity on esophagus cancer cell line EC109 in vivo, which is related to its role in decreasing of the expressions of c-FLIP mRNA and protein, inhibiting the growth of the transplantation tumor and inducing the apoptosis of cancer cells.
    Effects and mechanisms of VPA on cell apoptosis in the human hepotoma cell-HepG2
    SHI Chang-wen,ZHAO Xia,LI Jie,CAO Li-li,SUN Jing-jie
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1239-1242. 
    Abstract ( 1118 )   PDF (245KB) ( 677 )   Save
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    Objective To investigate the effects of Valproate acid sodium(VPA), a selective inhibitor of HDACs, on cell apoptosis of human hepotoma cell -HepG2 and study the probable mechanisms. Methods Apoptosis rate was determined by FCM with Annexin Ⅴ/PI staining. The activities and protein expressions of Caspase3, Caspase8 and Caspase9 were determined by spectrophotometry and indirect immunofluorescence techniques. ResultsBeing treated with different concentrations of VPA, the apoptosis rate of human hepotoma cells significantly increased in a time and dosedependent manner. The activities and protein expressions of Caspase3 and Caspase9 were upregulated in the experimental group compared with the control group (P<0.001). On the other hand, both the activity and the protein expression of Caspase8 were not significantly different between the experimental group and the control group (P>0.05). Conclusion VPA can effectively induce the HepG2 cell apoptosis by activating the intrinsic apoptosis pathway.
    Effect of Urokinase on bleomycin-induced pulmonary fibrosis and the expression of transforming growth factor-β1 in rats
    WAN Bing,ZHENG Jin-xu,HUANG Xin-jian,YAN Yu-lan,WU Jian-nong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1243-1246. 
    Abstract ( 1280 )   PDF (276KB) ( 772 )   Save
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    Objective To study the effects of urokinase on bleomycininduced pulmonary fibrosis in rats and its possible mechanism. Methods 45 male SD rats were randomly divided into three groups: the model group, the control group and the urokinase treatment group. The rats were intratracheally instilled with bleomycin(5mg/kg)or sterile saline. From the second day after bleomycin or sterile saline challenge, by intraperitoneal injection every two days, the urokinase treatment group was given urokinase (27500U/kg), the other two groups were given sterile saline. All the rats were sacrificed on day 28. Histological changes of the lungs were determined by HE staining and Masson′s trichrome staining. The level of transforming growth factor-β1 in BALF was determined by ELISA. Results At day 28, the neutrophil proportion in BALF was 3.1±1.8 in the treatment group, which was significantly lower than that of the model group（9.3±4.1） and higher than that of the control group(2.5±1.3) (P＜0.01)；the score of alveolitis was 0.9±0.7 in the treatment group, which was significantly lower that in the model group (1.7±0.6) (P＜0.01) but was higher than that in the control group(0.2±0.4) (P＜0.05). The pulmonary fibrosis was significantly decreased in the treatment group compared with the model group ［(1.1±0.7) vs (2.0±0.8), P＜0.01). The transforming growth factor-β1 level in BALF in the treatment group also significantly decreased compared with the model group ［(42.5±1.7)ng/ml vs (45.6±0.6), P＜0.01). ConclusionsUrokinase alleviates the bleomycininduced pulmonary fibrosis in rats possibly by inhibiting the expressions of transforming growth factor-β1 in lung.
    Protective effect of ginseng total saponin on the neuroal injury in rat models with focal cerebral ischemic reperfusion
    ZHANG Dong-jun,LI Yi-zhao,WU Xiao-yan,ZHANG Zhen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1247-1252. 
    Abstract ( 1159 )   PDF (336KB) ( 544 )   Save
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    Objective To investigate the protective effects of ginseng total saponin on brain tissue injury following ischemiareperfusion in rats with brain ischemia-reperfusion. Methods40 adult male Wistar rats were randomly divided into 5 groups: the control, the sham, the ischemic, the ginseng total saponintreated 1, and the ginseng total saponin-treated 2 groups. The rats received either ginseng total saponin (50mg/kg of body weight.) or saline i.p. just before the beginning of reperfusion and the brains were removed after 48h, and the ginseng total saponintreated 2 group was subjected to ginseng total saponin for 3 days and their brains were removed after 72?h. The brain tissues were observed by Nissle staining and TUNEL staining, and the data were calculated by using the image analysis system. ResultsIn the ischemic group, the number of neurocyte cells obviously decreased. TUNELpositive cells were not found in the normal rats but were found in the brain tissues of the sham and the ginseng total saponintreated groups. However, TUNEL-positive cells were found in the ischemic group. Deep brown staining was found in the nuclei of hippocampal and cortical neurons. Ginseng total saponin resulted in significantly fewer TUNELpositive cells in the neurons compared with the ischemic group (P<0.01). Both the and 2 groups had higher neuronal function values than the ischemic reperfusion nontreatment group (P<0.01), and the ginseng total saponin-treated 2 group was superior to the 1 group (P<0.05). ConclusionsGinseng total saponin may restrain the apoptosis of hippocampal neurons and increases the neuronal function value of focal cerebral ischemicreperfusion rats, and well protects the neurons from ischemiainduced apoptosis.
    Expression of TGFβ1 and BMP-2 in the experimental ligamentum flavum of the unstable cervical spine
    ZHANG Xi-shan,CAI Guo-dong,LI Jian-min,YANG Ming-feng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1253-1256. 
    Abstract ( 1202 )   PDF (266KB) ( 613 )   Save
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    Objective To study the effects of the unstable cervical spine on transforming growth factor-β1(TGFβ1) and bone morphogenetic protein-2 (BMP2) of the ligamentum flavum in rats. MethodsTotal RNA was prepared from ligamentum flavum of the experimental segment of different groups and the expressions of TGFβ1 and BMP2 was determined by reverse transcriptase polymerase chain reaction using specific primers. Result The gene expression of the unstable cervical spinegroup was higher than that of the control groups in each time spots. Conclusion The expression of TGFβ1 and BMP2 in the ligamentum flavum in rats with unstable cervical spine is different by time spots, which may accelerate the degeneration of the ligamentum flavum.
    Multi-slice computed tomography imaging for congenital sensorineural hearing loss in infants
    BIAN Zhi-qiang,LIU Ya-qun,LI Du-min,CUI Feng-yu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1257-1260. 
    Abstract ( 1214 )   PDF (264KB) ( 684 )   Save
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    Objective To discuss the value of multislice computed tomography imaging (MSCT) in infants with congenital sensorineural hearing loss（SNHL）. Methods74 infants with SNHL were subjected to MSCT. Axial highresolution MSCT scan and MPR reconstruction were performed on in all patients. The 3D reconstructions were performed on with the volume rendering technique on the workstation. Results 42 patients (84 ears) were normal and 21 patients (42 ears) had congenital inner ear malformations（of which 29 ears were accompanied by other malformations）. Of the 63 patients with prespeech deafness, 1 patient (2 ears) had incomplete partition type I deafness, 4 (8 ears) had incomplete partition type II, 12 (24 ears) had vestibulelateral semicircular canal dysplasia, 13 (26 ears) had large vestibular aqueduct，3 (3 ears) had internal auditory canal abnormality， and 1 (1 ear) had bony external auditory canal absence. Of the malformations, all the axial, MPR and VRT images could display the site and degree in 37 ears. VRT images were superior to the axial images in displaying the malformations in 5 ears with Mondini malformation and 12 ears with secretory otitis media. Of the 11 patients with postspeech deafness, 8 patients (16 ears) were proved be normal， 3 patients (6 ears) had large vestibular aqueduct syndrome, 3 patients (6 ears) had secretory otitis media. Conclusion MSCT allows a comprehensively assessing for various congenital ear malformations by high quality MPR and VRT reconstructions，and it plays an important role in diagnosis of infant SNHL.
    Diagnosis of perianal fistula with different MR sequences at 3.0 Tesla
    LI Ying,LI Chuan-fu,DAI Yong,MENG Xiang-shui,KANG Xiao-shui
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1261-1263. 
    Abstract ( 1407 )   PDF (235KB) ( 793 )   Save
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    ObjectiveTo explore the clinical value of different MR sequences in diagnosis of perianal fistula. MethodsMRI was performed on fifteen cases of clinically suspected as perianal fistula. The MR pulse sequences included sagittal T2 WI (SAG T2 WI), coronal T2 WI with fat suppression (COR T2 FS)， axial T1WI(AX T1WI), axial T2WI(AX T2WI), axial T2WI with fat suppression(AX T2 FS) and dynamic contrast enhancement(DCE). The MR findings were compared with the surgical results. Results16 internal opening and 14 tracks were found in 15 cases. Based on John Morris′s study, 3 cases were grade1, 6 cases were grade2, 3 cases were grade3, 2 cases were grade 4, and 1 case was grade 5. 2 cases only had perianal abscess. The internal opening display rate of the DCE imaging was much higher than that of other sequences, but there were no statistical differences between the DCE and AX T2 FS（P＞0.05）. The fistula track display rate of the DCE imaging was also much higher than that of the other sequences, and there were statistical differences only between the DCE and AX T1WI（P＜0.05). ConclusionOptimized coronal T2 fat suppression and dynamic contrast enhanced MR imaging are critical for perianal fistula, which provides great help for surgical planning and treatment.
    Color doppler ultrasonography in microsurgeries for deep supratentorial gliomas
    WANG Xing-qiang,XIONG Feng,DING Wei,JIE Lian-li,LIU Qing-hua,LIU Qing-lin,LI Gang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1264-1266. 
    Abstract ( 1430 )   Save
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    Objective To explore the effects of color Doppler ultrasonography in microsurgeries for deep supratentorial glioma. MethodsBefore removing the tumor under the microscope, color Doppler ultrasonography was used to locate the right position of tumor epidurally or subdurally. ResultsA comparison between the images of pre and postoperation showed that tumors were right positioned in 36 cases. 16 cases had total resection under microscopy, 12 had subtotal resection, and 8 had partial resection. After the operations, neurological impairment was aggravated in 4 cases, hemiparalysis was aggravated and recovered later in 3 cases, and motor aphasia was aggravated in 1 case in a short term. Conclusion Color doppler ultrasonography can be used to locate the position of tumors and decreases the sideeffects in a largest degree.
    Wavefront aberration in children with refraction amblyopia
    YANG Jun,ZHANG Xiao,CAO Qian-zhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1267-1270. 
    Abstract ( 1195 )   PDF (226KB) ( 624 )   Save
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    Objective To study the characteristics and role of wavefront aberrations in children with refractive amblyopia. Methods 83 children with refractive amblyopia were involved. After using 1% atropine ointment, once a day for three!days, optometry was determined by retinoscopy optometry and computer optometry, then the wavefront aberrations were determined by Allegretto wavefront analyzer after 20 days. The characteristics and disturbs of all kinds of high order wavefront aberrations in amblyopia patients of refraction error were observed, and the relationship between amblyopia and wavefront aberration was also observed. ResultThe total root mean square (RMS) values were significantly related to the refractive power in diopters (P＜0.05), and the aberrations of trefoil, coma and sperical increased with an increase of the astigmatism power. Conclusion Aberrations increase with an increase of refractive power in diopters and astigmatism is significantly related to the high aberrations. Analysis of the high order aberrations is helpful in discovering the cause of refractive astigmatism.
    Effect of pilocarpine on apoptosis of corneal endothelium in rabbits
    TAO Yuan,WANG Hong,QIAO Zhi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1271-1274. 
    Abstract ( 1243 )   PDF (285KB) ( 652 )   Save
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    Objective To investigate the effects and mechanisms of pilocarpine on corneal endothelium in rabbits. Methods20 rabbits in the experiment group were subjected to 2% pilocarpine 3 times a day for 1 month or 3 months. The expressions of bcl-2 and bax protein were determined by Westernblot, the apoptosis rate of rabbit corneal endothelium cells was determined by flow cytometry, and the morphological changes of the corneal endothelium cells were determined by transmission electron microscopy. ResultsThe expression of bax protein in the experiment group was higher than that in the control group, however the expression of bcl-2 protein was lower than that in the control group. The apoptosis rate of the rabbit corneal endothelium cells using pilocarpine for 3 months was significantly higher than that of the control group (P＜0.05). Also the transmission electronic microscopy revealed there were morphological characteristics of apoptosis including swelling of organelles, increasing of vacuoles in the cytoplasm, compaction and margination of nuclear chromatin in the groups after using pilocarpine for 1 month and 3 months. Conclusion Topical use of 2% pilocarpine can induce the apoptosis of corneal endothelium cells in rabbits.
    Effects of didanosine on the outgrowth of dissociated cultured dorsal root ganglion neuronal
    LIU Hua-xiang,LIU Zhen,YANG Xiang-dong,WANG Li-hong,LI Zhen-zhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1275-1277. 
    Abstract ( 1171 )   PDF (249KB) ( 548 )   Save
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    Objective To investigate the effects of didanosine (ddI) on the outgrowth of dissociated cultured dorsal root ganglion (DRG) neuronal. MethodsDissociated cultured DRG cells of the rat embryos were treated with ddI at different concentrations (1μg/mL, 5μg/mL, 10μg/mL and 20μg/mL, respectively). After 3 days exposure to ddI, the dissociated DRG cells were processed by microtubule associated protein 2 (MAP2) immunofluorescent labeling and the changes of neuronal cell body and neurites were observed by confocal laser scanning microscopy (CLSM). ResultsThe number of neurites in each neuron was decreased and the length of neurites in each neuron was shortened after 3 days treatment with ddI as compared with those in the control group. Whereas alterations of the diameter of neuronal cell bodies were not observed compared with those in the control group. ConclusionddI directly effects the outgrowth of neurites of the DRG neurons but dose not cause neuronal cell body shrinking in dissociated DRG cell cultures.
    Association of single nucleotide polymorphism of the C1040T gene encoding TAFI with myocardial infarction
    WANG Li-li,XU Cheng-wei,YU Chao,ZHAO Jing-jie,WU Xiao-ben,JIANG Cui-ying,DU Yi-meng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1278-1281. 
    Abstract ( 1346 )   PDF (254KB) ( 428 )   Save
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    Objective To explore the association between encoding gene CPB2 polymorphism of thrombin activatable fibrinolysis inhibitor（TAFI）and myocardial infarction. MethodsPCRRFLP (Restriction fragment length polymorphism) method was used to determine the CPB2 gene polymorphism in patients with myocardial infarction（n=100） and healthy controls（n=90）. ResultsThe CPB2 genotyping（Thr/Thr，Thr/Ile and Ile/Ile） frequencies were 32（32%），53（53%）and 15（15%）in the MI group and 31（34.4%），49（54.4%），10（11.2%）in the control group, and the allelic frequencies（C and T）were 117（58.5%）and 83（41.5%） in the MI group and 111（61.7%）and 69（38.3%）in the control group. χ2 analysis showed nonsignificant differences in the Thr325Ile polymorphism distributions（χ2=0.6482，P=0.7232；χ2=0.3958，P=0.5292）；genotype and alleles distribution frequencies were in accordance with the HardyWeinberg equilibrium. ConclusionThe polymorphisms of TAFI gene are not related to myocardial infarction.
    Clinical effect of the plasma BNP levels in hypertensive patients
    LI Yu-xiang, LI Ji-fu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1282-1284. 
    Abstract ( 1304 )   PDF (254KB) ( 447 )   Save
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    Objective To explore the correlation between plasma BNP and left ventricular hypertrophy (LVH) and left ventricular diastolic dysfunction (LVDD) in patients with hypertension. Methods Eightyfour patients were divided　into the LVH group and the control group based on the LVMI, also they were divided into the LVDD group and control group based on the LVDD. Results The BNP levels were positively correlated with LVMI and LVPWT (r=0.74 P<0.01; r=0.61 P<０.０1) and were negatively correlated with E/A and FPV (r=-0.66 P<0.01; r=-0.65, P<0.01). Conclusions BNP is a valuable biochemical quota for predicting LVH and LVDD in patients with normal left ventricular function.
    Resistin serum levels in different groups and related factors
    XIAO Zheng-da,ZHANG Rong-hua,FEI Hong-hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1285-1288. 
    Abstract ( 1262 )   PDF (263KB) ( 887 )   Save
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    Objective To investigate the role of resistin in obesity and type 2 diabetes mellitus. Methods 55 patients with type 2 diabetes mellitus and 34 healthy controls were randomly enrolled in this study. No dysfunctions of heart, liver or kidney or other endocrine diseases were found. Based on plasma glucose and BMI, they were divided into four groups: the normal control group, the obese control group, the nonobese T2DM group, and the obese T2DM group. The levels of serum resistin, HbAlc, bloodfat, fasting plama glucose, and fasting insulin were determined. The insulin resistance index was calculated based on the HOMA model(HOMAIRI=FINS×FPG／225). Results The serum level of resistin of the obese T2DM group ［(1402±468)ng／mL］ was significantly higher than that of the other three groups (P＜0001). The serum resistin levels of the obese group［(814±284)ng／mL］ and the nonobese T2DM group［(856±502)ng／mL］ were significantly higher than that of the normal control group［(562±156)ng／mL］ (P＜005, P＜001). There were positive correlations between the level of serum resistin and the levels of HbA1c, LDL, IRI, SBP and BMI(r=0222,　r=0233,　r=0286,　r=0407,　 r=0480, respectively), and negative correlations between serum resistin and HDL and FINS (r=-0198, r=-0138, respectively). Stepwise regression analysis showed that BMI, HbA1c and systolic blood pressure highly influenced the serum level of resistin. Conclusion Resistin is an important factor linking obesity and type 2 diabetes mellitus. It may play a role in insulin resistance and obesity. Resistin is not only related to the metabolism of blood glucose and lipids, but also related to hypertension.
    Protective effect of propofol on brain following the whole body hyperthermia in rabbits
    WANG Zhi-gang,WANG Zhou,ZHAO Xin,BU Xiang-mei,FENG Chang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1289-1293. 
    Abstract ( 1204 )   PDF (332KB) ( 688 )   Save
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    Objective To observe the changes of LAC, SOD and MDA in blood and the apoptosis in brain of rabbits following a 42℃ whole body hyperthermia for 2 hours and to explore its mechanisms of propofol in protecting the acute brain injury. MethodsRabbits were randomly divided into four groups: the normal control group (A group), the model group (B group), the 5mg·kg-1·h-1 propofol group (C group) and the 10mg·kg-1·h-1 propofol group (D group). The B, C and D groups were irradiated by a heat instrument at about 42℃ for 2 hours. Then the levels of SOD, MDA and LAC were determined by apparatus, the pathological changes of brain tissues were observed by HE coloration, the nerve cell apoptosis in brain cortex was determined by TUNEL method, and the relationship between the nerve cell apoptosis and the SOD and MDA levels at a resumed moment anus temperature was analyzed by pertinency method. ResultsThere were significant differences among the A, B, C and D groups in the levels of SOD, MDA and LAC, the number of apoptosis cells and the injury degree of HE coloration　（P<0.05 or P<0.01），SOD：A group >D group >C group>B group； MDA, LAC, the number of apoptosis cells, the injury degree of HE coloration：A group
    Clinical analysis of 20 cases gastrointestinal mucosaassociated lymphoid tissue lymphoma
    WU Kai, LI Nan, ZHANG Lin,ZHAI Jun-shan,WANG Xin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(12):  1294-1296. 
    Abstract ( 1244 )   PDF (240KB) ( 668 )   Save
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    Objective To summarize the experiences in diagnosis and treatment of mucosaassociated lymphoid tissue (ＭＡＬＴ) lymphoma. MethodThe clinical data of 20 cases of MALT lymphoma treated in our hospital in five years were retrospectively analyzed. Results① Most of the patients were more than 50 years old, and the male patients were more than the female patients. ②MALT lymphoma had no significant characteristics under endoscopy. ③MALT lymphoma should be operated, and if patients lost the opportunity of operation, chemotherapy may used to improve their prognosis. ④The classical CHOP plan was effective and economical. ⑤A few ring cells could be found in pathological tissues and it should be differentiated with ring cell adenocarcinoma. Conclusion Gastroenterologic MALT lymphoma has no typical clinical symptoms but it is rising in recent years, so it should be taken seriously by surgeons.
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