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Table of Content

      
    24 June 2007
    Volume 45 Issue 6
    Articles
    Expression and significance of lectin WGA, RCA and ECL receptors in the development of the mouse neural tube
    ZHANG Hui,LIU Kai,HE Xiu-quan,ZHANG Yan-ping
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  541-545. 
    Abstract ( 1320 )   PDF (255KB) ( 687 )   Save
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    Objective: To explore the relationship between neural tube development of the mouse embryos and lectin bindings of WGA, RCA and ECL. Methods: Thirty pregnant mice were randomly divided into six groups. Then biotin labeled WGA, RCA and ECL were used as probes to investigate the distribution and dynamic changes of their receptors in embryonic neural tubes by the affinity cytochemistry method and image analysis. Results: These three kinds of lectin receptors existed in the mouse embryonic neural tube at different developmental stages, but both the expression sites and the expression level of these three kinds of lectin receptors were quite different. The general tendency of expression of WGA and ECL receptors in the neural tube at different developmental stages was gradually decreased and then gradually rose. The general tendency of expression of RCA receptor in the cranial and caudal parts of embryonic neural tube at different stages rose, but that of the lectin RCA in the middle part of the neural tube at different stages decreased. The tendency of these lectin receptors, especially the WGA and RCA receptors, in the cranial and caudal parts of the murine neural tube were always higher than that in the middle part of the neural tube. Conclusion: These three kinds of lectin receptors, especially the WGA and RCA receptors, have a relation to the morphogenesis of the embryonic neural tube. Expression of three lectin receptors takes on the changes of regularity in embryonic neural tube development.
    HLA allele polymorphism in northern Hans of China
    SONG Yong-hong,MA Chun-hong,LV Hong-juan,ZHU Chuan-fu,NIE Xiang-min,WANG Mei,LIU Yan,ZHANG Ping
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  546-553. 
    Abstract ( 1456 )   PDF (269KB) ( 753 )   Save
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    Objective: To analyze the polymorphism of HLA-A,-B, and -DRB1 in northern Hans of China and obtain more comprehensive and accurate data about HLA molecular genetics. Methods: The methods of polymerase chain reactionsequence specific primers and sequence specific oligonucleotide probing(PCR-SSP and -SSOP) were used to determine the HLA-A,-B, and -DRB1 alleles in 8924 unrelated healthy Han individuals in northern China. Results:Low resolution alleles 18 HLA-A, 44HLA-B and 13 HLA-DRB1 including some rare HLA alleles that had not been reported in China before were discovered in this study. The most prevalent HLA types were A*02, B*13 and DRB1*15, with a gene frequency of 0.2886, 0.1430 and 0.1759 respectively. The most common A-B haplotype in this study was A*30B*13 with a haplotype frequency of 0.0895. The most common HLA-A-B-DRB1 haplotype was A*30B*13DRB1*07 with a haplotype frequency of 0.0742. Conclusion: HLA-A, -B, and -DRB1 alleles in the northern Hans of China are high polymorphic. Large scale DNA based on HLA genotyping contributes to the detection of low frequency alleles.
    Package and expression of lentivirus encoding human GCSF genes
    LIN A-li,SUN Qing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  554-557. 
    Abstract ( 1332 )   PDF (262KB) ( 878 )   Save
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    Objective: To construct a lentiviral vector encoding human granulocytemacrophage colony stimulating factor(hGM-CSF) genes and to transfect CT26 cells in order to establish the CT26 strains which has been continuously overexpressing the hGM-CSF gene. Methods: DNA fragments of human GM-CSF were amplified by PCR and were inserted into the vector plasmid L166. The three plasmids expressing lentivirus were packaged into virus packaging cell line 293T using the CaCl2 method. After 72 hours of transfection, the virusproducing cell supernatant was produced. CT26 cells were transfected by lentivirus and the titration was determined. Results: The sequence of the PCR product in the recombinant plasmid was produced. The three plasmids were effectively transferred into 293T. When the CT26 cells were infected as target cells by the lentivirus supernatant, the titration of lentivirus was 4.69×105IU/ml. hGM-CSF was determined in the supernatant of CT26 cells transfected by lentivirus, which was kept for more than two months. The transfected CT26 cells were screened by puromycin at a terminal concentration of 15μg/ml. Conclusion: The lentivirus encoding hGMCSF genes were successfully constructed.
    Effect of 2-methoxyestradiol on proliferation and apoptosis of neurons and C6 glioma cells in rats
    ZHANG Hong-ri,LI Gang,ZHAO Yong-gang,CHENG Xiao-bing,PENG Jing-hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  558-560. 
    Abstract ( 1073 )   PDF (241KB) ( 390 )   Save
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    Objective: To investigate the effect of 2-methoxyestradiol on growth inhibition and apoptosis of C6 glioma cells and neurons in vitro. Methods: C6 glioma cells and neurons were divided into the control group and four 2-methoxyestradiol treatment groups. C6 glioma cells and neurons were treated by different doses of 2-methoxyestradiol for different times and observed by microscopy and electromicroscopy. The viability was determined by MTT assay and the cellcycle and apoptosis were determined by flow cytometry. Results: 2-methoxyestradiol caused a dose and timedependent inhibition on the C6 glioma cells viability(P<0.05). The population of C6 glioma cells in the G1 and S phase was decreased while in the G2 phase it wasincreased by 2-methoxyestradiol in a dose and time-dependent manner(P<0.05); However, 2-methoxyestradiol had no effect on neurons. Conclusion: 2-methoxyesradiol can inhibit proliferation and induce apoptosis of C6 glioma cells, but has no effect on neurons.
    Aparoscope or/and ventriculoscope in the treatment of hydrocephalus
    SUN Jin-long,ZHANG Qing-lin,XI Guang-ming,SHEN Shui-jun,LI Wei-guo,SONG Qian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  561-564. 
    Abstract ( 1111 )   PDF (283KB) ( 724 )   Save
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    Objective: To explore the effect of aparoscope or/and ventriculoscope on hydrocephalus. Methods: Ninetythree cases of hydrocephalus were treated by aparoscope or/and ventriculoscopic techniques. Among them, 52 cases were subjected to ventriculohepatodiaphramatic space shunt under aparoscope; 41 cases were treated under ventriculoscope, of them, 23 cases were subjected to septa pellucidum fistulation, 18 cases to choroid plexus electrofulguration, 18 cases of occlusive hydrocephalus neuroendoscopic third ventriculostomy, 11 cases of cyst fistulation in ventricle, 8 cases to adjustment of the shunt channel ventricle tip, 7 cases of saddle cystic tumor combined with hydrocephalus neuroendoscopy, 5 cases of ventriculoperitoneal (V-P) shunt by neuroendoscopy combined with laparoscopy, and 3 cases of pineal tumor combined with hydrocephalus neuroendoscopy. Results: After a followup of 3 months to 4 years, the ventriculohepatodiaphramatic space shunt for hydrocephalus under aparoscopy was superior to routine ventriculoperitoneal shunt for hydrocephalus. Of the 41 cases treated by ventriculoscopy, 35 improved and 6 did not. Conclusion: Most hydrocephalus can be treated by neuroendoscopy or ventriculoscopy associated with aparoscopy.
    Expression of ACT A in neonatal rats with hypoxic ischemic brain damage
    WANG Xiao-qin,AN Li,WANG Ping,ZHENG Yuan,ZHAO Dong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  565-568. 
    Abstract ( 1065 )   PDF (275KB) ( 681 )   Save
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    Objective: To investigate the expression of activin A (ACT-A) in brain tissues of neonatal rats with hypoxia and ischemia (HI). Methods: The left common carotid arteries of neonatal rats at 7 days in the hypoxic ischemic brain damage(HIBD) group were ligated, and then they were subjected to 8% oxygen for 2 hours. Expressions and origins of ACT-A were determined by immunohistochemical and histopathologic methods. Results: Positive ACT-A cells were found in the left cingulum cortical area 1h after HI, and obviously increased compared with those in the control group and in the contralateral area [(2.50±1.51) vs (1.13±0.64),and (2.50±1.51) vs (1.00±0.76), P<0.05]. Expression of ACT A reached a peak at 10 hours after HI. Large amounts of positive ACT A cells were found in the left cingulum cortical, corpus striatum, dentate gyrus, and were obviously increased compared with that in the control group [(19.75±2.19) vs (1.25±0.71), P<0.01] and in the contralateral area [(19.75±2.19) vs (1.00±0.76), P<0.01]. Overexpression of ACT-A was found till 72 hours after HI [(12.75±3.62) vs (1.38±0.74), and (12.75±3.62) vs (1.00±0.76), P<0.05]. Conclusion: Hypoxiaischemia strongly induces the expression of ACT-A. This suggests that ACT-A may play an important role in the pathogenic process of HIBD in newborn rats.
    The allele polymorphisms of HLA class I (HLA-A and -B ) of the umbilical cord blood of 5844 Shandong people
    DAI Yun-peng,YAN Wen-ying,SHEN Bai-jun,WANG Hong-mei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  569-572. 
    Abstract ( 1167 )   PDF (251KB) ( 614 )   Save
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    Objective: To investigate the HLA-A and -B allele polymorphisms in the umbilical cord blood of Shandong people. Methods: 5844 umbilical cord blood samples were drawn from the Shandong umbilical cord bank and determined by the polymerase chain reactionsequence specific oligonucleotide (PCR-SSO) method. Results: Twenty alleles at HLAA locus and fortysix alleles at HLAB locus were detected. Of the twenty HLAA alleles, the most prevalent five alleles were A*02(0.3041), A*11(0.1443), A*24(0.1434), A*30(0.0975) and A*33(0.0859), and the least prevalent alleles were A*34(0.0006), A*25(0.0005, A*66(0.0005), A*74(0.0004) and A*36(0.0001). Of the fortysix HLAB alleles, the most prevalent five alleles were B*13(0.1348), B*51(0.0713), B*62(0.0712), B*61(0.0676)and B*60(0.0642), and the least prevalent alleles were HLAB*77(0.0001), B*76(0.0002), B*47(0.0003), B*42(0.0003)and B*72(0.0004). Comparing the HLA-A and -B gene frequencies of Shandong umbilical cord blood samples with those of other Han Chinese, there were unique distribution features of the HLA-A and -B alleles among the populations studied from various regions with the same racial origin. The differences from various regions of the same race were fewer than those from different races. Conclusion: The HLA-A and -B alleles in Shandong umbilical cord blood have a rich polymorphism, which represents the distribution characters of the HLA-A and-B alleles in Shandong Hans. For a patient of northern Han Chinese it is easy to search for a HLA-A or -B matched cord blood donor from the Shandong umbilical cord blood bank. For a patient of southern Chinese it is possible to receive a transplant of cord blood stem cells matched with HLA-A or B from the Shandong umbilical cord blood bank.
    Effect of tamoxifen on the proliferation and expression of ER and Smad4 in human hepatocellular carcinoma cells HepG2
    BAI Wen-kun,WANG Wen-qi,SHI Chang-wen,WU Hong-wen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  573-576. 
    Abstract ( 1178 )   PDF (244KB) ( 645 )   Save
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    Objective: To study the effect of tamoxifen on the proliferation and expression of the estrogen receptor(ER) and Smad4 in human hepatocellular carcinoma cells HepG2. Methods: Three experimental groups were subjected to 7.5μmol/L, 15μmol/L,  and 30μmol/L tamoxifen. The control group was not subjected to tamoxifen. Each group contained six wells, and there were 0.1ml containing 1×105/ml cells in each well. After being observed for 24h, 48h, and 72h, the inhibiting rate of HepG2 cells was determined by MTT. The expression of ER and Smand4 in HepG2 cells was determined by immunohistochemistry. Results: Tamoxifen inhibited the proliferation of human hepatocellular carcinoma cells in a time and dose dependent manner. It also suppressed the ER expression but raised the Smad4 expression in a time and dose dependent manner. Conclusion: Tamoxifen can suppress human hepatocellular carcinoma cell proliferation by affecting the expression of ER and Smad4 in HepG2 cells.
    Effect of CTGF shRNA on expressions of TGFβ1, CTGF mRNA and extracellular matrix secretion in rat hepatic stellate cells
    ZHU Yu-hua,LI Xiao-hong,ZHANG Chun-qing,REN Wan-hua,ZHAO You-an,MA Yan-li
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  577-581. 
    Abstract ( 1237 )   PDF (280KB) ( 591 )   Save
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    Objective:To investigate the effect of CTGF short hairpin RNA(shRNA) on the expression of TGF-β1, CTGF, type Ⅰ procollagen, type Ⅲ procollagen mRNA and extracellular matrix secretion in HSC-T6. Methods: The CTGFshRNA plasmids containing EGFP successfully constructed and screened, were transfected to HSC-T6 with the transfection reagent Metafectene. There were another three HSC-T6 groups: one with plasmids and without CTGFshRNA, one with only Metafectene, and one untreated. The expression of TGF-β1, CTGF, type Ⅰ procollagen and type Ⅲ procollagen mRNA levels were determined by reverse transcriptionpolymerase chain reaction (RTPCR) after 24 and 48 hours. The content of precollagen type Ⅲ, Ⅳtype collagen, and hyaluronate (HA), laminin (LN) in the supernatants were determined by radioimmunoassay. Cell transfection efficiency was analyzed by flow cytometry. Results: The transfection efficiency of CTGFshRNA plasmids in HSC were (60±5)%, (42±3)% at 24 and 48 hours respectively.Compared with those of the blank control, there was no difference in the expression of genes and extracellular matrix secretion in HSC-T6 in the other groups, the group with plasmids and without CTGF shRNA and the group with only Metafectene. Expression of CTGF, type Ⅰ collagen and type Ⅲ collagen mRNA were markedly downregulated in CTGFshRNA transfected HSC-T6(P<0.01 or P<0.05). But there was no difference in the expression of TGF-β1 mRNA. The contents of precollagen type Ⅲ, Ⅳtype collagen, hyaluronate (HA) , and laminin (LN) in the supernatants decreased significantly in CTGFshRNA transfected HSCT6(P<0.01 or P<0.05). Conclusion: CTGFshRNA can regulate the gene expression of the growth factor and the extracellular matrix molecule and inhibits the extracellular matrix secretion in HSCT6.
    Effect of interferon on the apoptosis in the hepatoma cell line HepG2.2.15
    ZHANG Jiao,LIU Qian,MAO Hai-ting,WANG Jie
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  582-585. 
    Abstract ( 1148 )   PDF (221KB) ( 716 )   Save
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    Objective: To investigate the immune escape of hepatoma cell line HepG2.2.15 with low Fas expression as well as the effect of interferonα(IFN-α) on the apoptosis in heptoma cells through upregulating Fas expression. Methods: Flow cytometry was used to determine the Fas expression of the hepatoma cell line HepG2.2.15 and that treated with IFN-α. Resistance of hepatoma cells to Fas mediated apoptosis and the effect of IFN-α on the apoptosis were studied using antiFas agonistic monoclonal antibody CH11. Results: ①Fas expression of HepG2.2.15 was low and increased after the cells were treated with IFN-α; ②CH11 could not induce HepG2.2.15 cells to apoptosis. But the apoptosis induced by CH11 increased when the cells upregulated the Fas expression after treatment with IFN-α. Conclusions: The hepatoma cell line HepG2.2.15 could resist Fasmediated apoptosis. IFN-α is able to upregulate the Fas expression and subsequently promotes the CH11mediated apoptosis .
    Structure and characteristics of the mechanosensitive receptors in rat gut
    YANG Xia,LI Yan-ing/sup>
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  586-589. 
    Abstract ( 1245 )   PDF (259KB) ( 542 )   Save
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    Objective: To investigate the structure and characteristics of mechanotransduction by intraganglionic laminar endings (IGLEs) as the mechanoreceptors in rat gut. Methods: ① IGLEs were displayed by styryl dye FM1-43 with stretch stimulation to the rat esophagus. P2X2 immunohistochemistry staining was used to confirm that the structure displayed by FM1-43 was that of IGLEs; ② Different drugs (TTX, Ca2+, Benzamil, Capsaicin, and α,β- meATP)were used to block or stimulate stretchevoked IGLEs staining by FM1-43 for further investigating the mechanism of mechanotransduction by IGLEs. Results: ① The amount of IGLEs displayed by stretchevoked FM1-43 staining was much more than that without stretch stimulation (P<0.01). The structure of IGLEs displayed by FM1-43 was consistent with that by P2X2; ② TTX, Ca2+ had no effect on stretchevoked IGLEs staining, while benzamil could significantly block it. Caqsaicin could activate the entrance of FM1-43 into IGLEs without any stretch, while α,β- meATP, and the potent ATP anologue could not stimulate the staining at rest status. Conclusion: ① IGLEs are the mechanoreceptors of vagal afferent endings in the gut; ② IGLEs transduce the mechanical stimulation in the gut through some mechanosensitive ion channels which are a type of sodium ion channels. Neurotransmitters or action potentials are not involved in this process.
    Effect of the HSV-TK gene with adenovirus mediation on osteoarthritic synoviocytes
    DING Yuan-jing,ZHANG Wei,LI Wei,ZHANG Lei,SUN Shui,WANG Jian,WANG Xian-quan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  590-594. 
    Abstract ( 1308 )   PDF (280KB) ( 695 )   Save
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    Objective: To investigate the effect of the HSVTK gene on adenovirusmediation on osteoarthritic synoviocytes. Methods: Cultured osteoarthritic synoviocytes were transduced with the adenovirus containing the HSV-TK gene. The expression of the adenovirus was determined. Cells without treatment served as the controls and the infected cells were treated with GCV. Then the inhibition ratio was determined. The preliminary morphological changes were observed by light microscopy and the nucleus subjected to fluorescent staining was observed by fluorescent microscopy. The proportion of necrosis and apoptosis was quantitatively analyzed. Results: The adenovirus had the ability to infect the cultured osteoarthritic synoviocytes. The proliferation of the treatment group was significantly inhibited (P<0.05). Necrosis and apoptosis were both found in transduced cells. Conclusion: The HSV-TK/GCV system significantly inhibits the proliferation of osteoarthritic synovial cells.
    Protective effects of grape seed proanthocyanidins on endothelial cells intervened by advanced glycosylation end products
    MA Li,GAO Hai-qing,LI Bao-ying,MA Ya-bing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  595-598. 
    Abstract ( 1087 )   PDF (282KB) ( 604 )   Save
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    Objective:To investigate the protective effect of grape seed proanthocyanidins (GSPC) on human umbilical vein endothelial cells (HUVEC) injured by advanced glycosylation end products (AGE) and its possible mechanism. Methods: AGEmodified bovine serum albumin (AGEBSA) was prepared by incubating the BSA with a high concentration of glucose. The cultured HUVECs were divided into six groups: the control group,the BSA group, the AGE group, the low dose GSPC +AGE group, the middle dose GSPC+AGE group, and the high dose GSPC+AGE group. The cell proliferation of HUVEC, the Von Willebrand factor (vWF) and nitric oxide (NO) were determined. Results: After treatment with AGE, the proliferation of HUVEC was significantly reduced. The proliferation rate was 90.53% in the control group, while the proliferations of cells pretreated with GSPC of different concentrations were 0.95fold, 1.12fold, and 1.23fold that of the control group. The vWF level in AGEtreated HUVEC was higher than that of the control group (P<0.01). Preincubation with GSPC of different concentrations could decrease the vWF level increased by AGE in a dosedependent manner. The NO content in the AGEtreated group was significantly lower than that of the control group(P<0.01), while the AGEdecreased NO level was significantly increased in the GSPCpretreated groups. Conclusion: AGE can inhibit HUVEC proliferation, injure HUVEC and decreased the NO content. GSPC could protect HUVEC against damage induced by AGE and increase the NO level in the AGEexposed HUVEC. It is possible that the increased NO plays a key role in protecting the GSPC against damage induced by AGE.
    Combination drugs delay renal interstitial fibrosis of rats with Adriamycin nephropathy
    LI Feng,ZHEN Jun-hui,FAN Xiao-hong,JIANG Bei,LI Xian-hua,GUO Ling,HU Zhao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  599-603. 
    Abstract ( 1302 )   PDF (286KB) ( 567 )   Save
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    Objective: To investigate the protective effect and regulating mechanism of Benazepril combined with Paecilomyces hepiali Chen on rats with renal interstitial fibrosis. Methods: Rats were randomly divided into three groups: the model group(M group), the Benazepril group(B group) and the combination drugs group(U group). 24hour urinary protein excretion and renal function were determined at 8 weeks. Renal pathology and immunohistochemistry were assayed to measure the expression of fibronectin(FN), collagenⅣ(ColⅣ), and the transformor growth factorβ1(TGF-β1). RT-PCR was used to examine the TGF-β1 mRNA, plasminogen activator inhibitor-1(PAI-1)mRNA, and tissue inhibitors of metalloproteinase-1(TIMP-1)mRNA. Results: Combination drugs and Benazepril effectively reduced the levels of 24hour urinary protein excretion, Bun, Scr, TG and Cho(P<0.01), but the levels of TP and ALB were significantly increased(P<0.01)compared with the M group. Furthermore, the levels of BUN, Scr, TG and Cho were also significantly reduced in the U group compared with the B group(P<0.05). The levels of 24hour urinary protein excretion, Bun, Scr, TG and Cho were significantly decreased(P<0.01) and the expression of LN, FN, ColⅣ, TGF-β1, TIMP-1 and PAI-1 was decreased by the combination drugs. Conclusions: Paecilomyces hepiali Chen combined with Benazepril can effectively delay the development of renal interstitial fibrosis.
    Transplantation of fetal bloodderived mesenchymal stem cells in the treatment of cerebral ischemia in rats
    JU Xiu-li,HUANG Zhi-wei,WEI Xin-bing,HOU Huai-shui,SHI Qing,LI Dong,SUN Nian-zheng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  604-608. 
    Abstract ( 1192 )   PDF (311KB) ( 620 )   Save
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    Objective: To observe the role of purified mesenchymal stem cells (MSCs) derived from fetal blood on the neurological function in rats with middle cerebral artery occlusion (MCAO). Methods: Thirty adult male Wistar rats were randomly divided into three groups: the operation group, the transplantation of MSCs group and the injection of saline group. Functional outcome measurements were performed based on the modified neurological severity score(mNSS) at day 1, 7, 14, 21 and 28 after MACO. The survival, migration, expression of Nestin, glial fibriliary acidic protein (GFAP) and neuronspecific enolase(NSE) of 5bromo2deoxyuridine(BrdU)-labeled MSCs were detected by immunohistochemical double staining. Results: MSCs from the fetal blood had a powerful proliferation ability. MSCs significantly improved the neurological function (P<0.05). The engrafted cells survived and migrated to the ischemic tissues. (2.88±0.76) %, (5.24±1.06)% and (1.34±1.29)% of the MSCs respectively expressed Nestin, GFAP and NSE. Conclusion: After proliferation and purification, the MSCs in the fetal blood greatly improved the neuro logical function in rats with cerebral ischemia, meanwhile they may survive, migrate and differentiate into astrocytes or neurons.
    Antiapoptotic effect of recombinant human growth hormone on myocardial ischemia/reperfusion injury in rats
    WANG Zhengjun,ZHANG Hai-zhou,ZOU Cheng-wei,LI De-cai,LI Hong-xin,FAN Quan-xin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  607-612. 
    Abstract ( 1237 )   PDF (293KB) ( 496 )   Save
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    Objective: To probe into changes of the cardiomyocyte apoptotic index and to observe the effect of recombinant human growth hormone(rGH) on cardiomyocyte apoptosis and mitochondria at different times after ischemicreperfusion(IR). Methods: Seventy-eight Wistar rats were randomly allocated into the IR group, the IR+rGHⅠgroup, the IR+rGHⅡ group and a sham operation group( without I/R injury). The IR group was given a normal sodium solution subcutaneously each night for 7 days before the experiment; the IR+GHⅠgroup was subcutaneously given 0.75U/kg recombinant human growth hormone each night for 7 days before the experiment; the IR+GHⅡgroup was given 1U/kg recombinant human growth hormone 30 seconds prior to ischemia. TUNEL was used to detect cardiomyocyte apoptosis. Results: The apoptosis cells appeared after 2 hours of reperfusion, increasing to a zenith after 24 hours of reperfusion and then decreasing. The difference of the apoptosis index between the IR group and the IR+rGHⅠ group was statistically significant(P<0.05), however, that between the IR group and the IR+rGHⅡ group was not. Conclusions: Recombinant growth hormone, used for 7 days before ischemicreperfusion can reduce the number of apoptosis cells and attenuate the damage to mitochondria, however, used 30 seconds prior to ischemia it does not have this effect.
    Evaluation of serum phospholipase A2 in patients with strangulated bowel obstruction
    CHEN Jiang-yi,LIU Jun-cheng,MA Shao-feng,CHEN Pu-qi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  613-615. 
    Abstract ( 1282 )   PDF (242KB) ( 452 )   Save
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    Objective: To explore the value of serum phospholipase A2(PLA2) in acute strangulated bowel obstruction. Methods: The serum PLA2 level was determined in different types of bowel obstruction, including strangulated obstruction, simple obstruction,torpid obstruction, and in controls by ELISA. Strangulated obstruction was subdivided into two groups: the ischemia group and the necrosis group. Their linear relations were statistical analyzed. Results: The mean serum concentration of PLA2 in the bowel obstruction group was significantly elevated compared with the healthy controls[(1.22±0.66) mg/L vs (0.17 ±0.02) mg/L] (t=9.05,P<0.001), the strangulated obstruction group was significantly higher than those of the simple obstruction group[(1.72±0.36) mg/L vs (0.57±0.24)mg/L (t=17.89,P<0.001), and the ischemia group was significantly higher than of the necrosis group[(1.96±0.14 )mg/L vs (1.54±0.37)mg/L (t=5.16,P<0.001). Conclusions: The serum PLA2 level correlates closely with the severity of obstruction. It has great value for early diagnosis of strangulated bowel obstruction and offers solid evidence for early treatment.
    Health economical evaluation of four thirdgeneration chemotherapy regimens in advanced nonsmall lung cancer patients in China
    HAO Jing,WANG Xiu-mei,YI Cui-hua,LI Li,WANG Xiu-wen,SUN Li-mei,YU Jin-ming
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  616-620. 
    Abstract ( 1345 )   PDF (279KB) ( 679 )   Save
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    Objective: To evaluate and compare the costs of four thirdgeneration chemotherapy regimens in advanced nonsmall lung cancer (NSCLC) patients in China. Methods: The Chinese biomedical database (CBMdisc) and Chinese Medical Current Contents (CMCC) were used to search for all literature from the year 1983 to 2005 which conformed to the following key words: vinorelbine, gemcitabine, paclitaxel, docetaxel and cisplatin and NSCLC. The corresponding four combination regimens were summarized and compared in response rate, overall survival and 3/4 degrees of adverse effects. Costminimization analysis was used according to the following cost model: CT(total cost)=C1 (chemotherapy)+C2 (drug administration)+C3 (concomitant medication)+C4(cost for 3/4 degrees of adverse effects). Sensitivity analyses were performed to quantify the effects of various cost drivers. Results: 18 out of 143 articles were available, and the response rates of the four combination chemotherapies in total for 721 patients were 47.11%, 48.50%, 43.28% and 37.82% (P>0.05) respectively for vinorelbine (NP), gemcitabine (GP), paclitaxel (PC), docetaxel (DC). Costs per cycle were respectively 4322.2RMB (NP), 7420.0 RMB (GP), 8113.8RMB (PC) and 6372.3RMB (DC). C1 covered the largest part in all regimens. Sensitivity analysis presented the conclusion that NP had the lowest cost and was not significantly affected by variance of C2, C3 and C4. Conclusions: Among the four thirdgeneration chemotherapy regimens in NSCLC, NP has the lowest cost, while PC is the most costly. Drug acquisition covers the largest part in all regimens.
    Comparison of flow-sensitive alternating inversion recovery and dynamic susceptibility constrast MR perfusion technique in evaluating intracranial tumor vascularization
    ZHANG Kai,LI Chuan-fu,LIU Ying,FENG De-chao,MENG Xiang-shui,ZHENG Jin-yong,CONG Pei-xin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  621-624. 
    Abstract ( 1259 )   PDF (261KB) ( 548 )   Save
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    Objective: To compare the effects of flowsensitive alternating inversion recovery (FAIR) and dynamic susceptibility contrast MR (DSCMR) perfusion technique in evaluating intracranial tumor vascularization. Methods: Thirtythree patients suffering from intracranial tumors were determined by using the FAIR and DSCMR perfusion techniques. The values of FAIR and CBV were recorded and analyzed by the linear correlation analysis method. The values of FAIR and CBV between benign and malignant gliomas were statistically analyzed. Results: There was a positive linear correlation between FAIR and CBV(r=0.450,P=0.009). The difference of the CBV value between benign and malignant gliomas was statistical (Z=-2.213,P=0.027). So was that of the FAIR value (Z=-2.021,P=0.043). Conclusion: FAIR does not need a contrast agent and could offer some comparable perfusion information to DSCMR technique to clinicians, so it has the potential to be clinically applied.
    MSCTA in evaluation of anatomical variation of hepatic arteries
    OUYANG Ai-mei,MA Xiang-xing,CUI Feng-yu,SU Xin-you,LI Du-min,WANG Qing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  625-628. 
    Abstract ( 1317 )   PDF (237KB) ( 574 )   Save
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    Objective: To discuss the types of anatomical variations of hepatic arteries. Methods: Hepatic arteriography was given to 242 patients by using the 16slice MSCT. The anatomical variations of hepatic arteries were analyzed and classified with Michels′ classification criteria. Results: Of these patients, 169 had normal heptic arteries (69.8%), 73 had variations (30.2%), and 25 had infrequent aberrant hepatic arteries not included in Michels′ classification (10.3%). Conclusion: The variation of hepatic arteries is complex. It is necessary for surgeons to evaluate hepatic arteries by MSCTA before interventional therapy, surgery and liver transplantation.
    Effect of Gluma desensitizer on tensile bond strength and shear bond strength of different dental cements
    LU Mei,GAO Ning,CEN Yuan-kun,JI Ping
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  629-631. 
    Abstract ( 1122 )   PDF (259KB) ( 385 )   Save
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    Objective: To evaluate the effect of Gluma desensitizer on bond strength of different dental cements in vitro. Methods: Twentyfour freshly extracted human molars were prepared to flatdentin and cemented at random to testing specimens made of NickelChromium alloy respectively by VitremerTM resinmodified glass ionomer cement and polycarboxylate cement (PC), twelve in each group. Six teeth of each group were treated by Gluma desensitizer before cementation. All samples were stored in distilled water at 37?℃ for 24 hours, and then they were tested on a universal testing machine (1?mm/min) for tensile bond strength (TBS). The above procedure was repeated for shear bond strength (SBS) with another twentyfour molars. Results: Application of Gluma desensitizer significantly increased the TBS and SBS of VitremerTM luting cement (P<0.05). The TBS and the SBS of PC were also slightly increased, but the changes in TBS had no statistical significance (P>0.05). Conclusion: Gluma desensitizer has no negative impacts on the bond strength of VitremerTM luting cement and PC.
    Role of rapamycin on cultured human Tenon's capsule fibroblasts
    ZHAO Jie,CAI Ke-li
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  632-634. 
    Abstract ( 1136 )   PDF (240KB) ( 630 )   Save
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    Objective: To explore the effect of rapamycin on the proliferation and differentiation of human Tenon′s capsule fibroblasts in cell cycles. Methods: The cultured fibroblasts were treated in DMEM medium containing various concentrations of rapamycin for 24 hours. The growth of fibroblasts was measured by a methylthiazlyl tetrazolium (MTT) assay. The flow cytometry method was used to evaluate the cell cycles. Results: Proliferation of fibroblasts were suppressed by rapamycin from a dose of 10?ng/ml to 160ng/ml(P<0.05). The cell growth inhibition rate treated with rapamycin concentrations of 10ng/ml, 20ng/ml, 40ng/ml, 80ng/ml, and 160ng/ml were 9.32%, 22.55%, 41.42%, 42.25%, and 43.57% respectively. The cell population was significantly increased at the G0/Gl phase from 41.76% to 44.19% and decreased at the S phase from 45.42% to 40.18% after the rapamycin treatment. Conclusion: Rapamycin is a potential inhibitor that blocks the proliferation of human Tenon′s capsule fibroblasts at the Gl restriction point.
    Preparation and quality control of penciclovir liposome
    SONG Li-jie,WANG Wei-hong,LU Jin-ling
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  635-638. 
    Abstract ( 1246 )   PDF (242KB) ( 477 )   Save
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    Objective: To study the preparation of penciclovir liposomes and its quality control. Methods: The optimum preparation technology was established by a uniform design. Negative staining and the laser scattering particle size distribution analyzer were used to study the structure and size of liposomes. A gel column and Kratio spectrophotometry were developed to determine the entrapment efficiency. Results: The average size of liposomes was 426.8nm. The entrapment efficiency was 60.76%. Conclusion: The preparation of penciclovir liposome is practicable and should be investigated further.
    Oral absorption effect of liposomes with different sizes on TFu
    SUN Wei-peng,ZOU Wei-wei,XI Yan-wei,ZHANG Na
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2007, 45(6):  639-642. 
    Abstract ( 1438 )   PDF (251KB) ( 357 )   Save
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    Objective: To investigate the oral absorption effect of liposomes with different sizes on TFu in mice. Methods: Based on former studies, TFu liposomes of different particle sizes (530nm, 320nm and 180nm) were prepared using the film dispersion-homogenization technique. The Zeta potentials and the polydispersity were evaluated by laser scatter analysis. The entrapment efficiencies were determined and the release characteristics in vitro were investigated. The mice were orally administered suspensions of TFu and liposomes of different particle sizes and venous blood was collected from the eyeholes of mice at a regular time. The plasma concentration of TFu was measured using the HPLC method and the pharmacokinetic parameters were calculated by the DAS(2.0) program. Results: The relative bioavailabilities of TFu liposomes with different particle sizes (530nm, 320nm and 180nm) were 160.5%, 221.6%, and 260.3%, respectively. Conclusion: The particle size of liposomes has a significant influence on oral absorption of TFu in mice: the relative bioavailability of liposomes increases with a decrease of particle size.