Loading...

Table of Content

      
    16 January 2008
    Volume 46 Issue 1
    Articles
    Articles
    Glial cell linederived neurotrophic growth factor promotes substance P release from cultured dorsal root ganglion neurons
    YANG Tao,LIU Zhen,WANG Li-hong,XING Zi-ying,WANG Huai-jing,LI Zhen-zhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  1-4. 
    Abstract ( 1231 )   PDF (513KB) ( 447 )   Save
    Related Articles | Metrics
    To explore the effects of glial cell linederived neurotrophic growth factor (GDNF) on substance P (SP) released from cultured dorsal root ganglion (DRG) neurons. MethodsDRG neurons of Wistar rat embryos were cultured without or with GDNF (5ng/mL, 50ng/mL) for 72h. The living cells were observed by an inverted contrast microscope. The neurons stained by microtubule associated protein 2 (MAP2) and 4′,6-Diamidino-2-phenylindole (DAPI) fluorescent were counted. The amount of SP basal release and of SP evoked by capsaicin was determined by radioimmunoassay (RIA). ResultsThe living status of DRG cells with GDNF incubation was better than that without GDNF incubation. The number of DRG neurons per visual field increased in GDNF treated cultures compared with that in cultures without GDNF. Both the amount of SP basal release and the amount of SP evoked by capsaicin significantly increased in GDNF treated cultures compared with those in cultures without GDNF. ConclusionGDNF may improve survival of cultured DRG neurons and promote SP basal release and sensitize the capsaicin evoked SP release from DRG neurons.
    Neuroprotective effects of Neprilysin on neuronal apoptosis induced by Amyloid-beta peptide
    ZHANG Ya-lun,DING Yan,SHAO Shi-bin,YANG Ling-ling,REN Kai,DUAN Shan,ZENG Ji-ping,CUI Xing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  5-8. 
    Abstract ( 1270 )   PDF (484KB) ( 478 )   Save
    Related Articles | Metrics
    To explore the protective effects of transfection of the Neprilysin gene on primary cells against apoptosis induced by Aβ25-35. MethodsPrimary cortical neurons of fetal Wistar rats in vitro were cultured and the apoptosis cell model was induced by Aβ25-35. The NEP genes were transiently transfected into neurons. Changes of cell apoptosis were determined by MTT assay and flow cytometric DNA analysis. The expression of target mRNA and proteins was determined by RT-PCR and Western blot. ResultsExposure of primary neurons to Aβ25-35 resulted in changes of cell apoptosis. The MTT assay showed that cell activity remarkably decreased (P＜0.01) and cytometric DNA analysis showed an increase in the apoptosis rate. While in the neurons transfected with NEP genes, there were no such remarkable changes when treated with Aβ25-35. The suppression effect of the NEP gene on APP expression was shown by RT-PCR and Western blot, and the expression of Bax was decreased while that of Bcl-xl was increased after transfection. ConclusionTransfection of the NEP gene can protect primary neurons from apoptosis induced by Aβ25-35.
    Proteomic analysis of cerebrospinal fluid in Demyelinative disease of the central nervous system
    QIN Yan-jiang,LIU Shi-lian,YANG Yin-rong,BAI Shu-mei,ZHANG Xu-hua,DENG Xiao-mei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  9-14. 
    Abstract ( 1207 )   PDF (506KB) ( 446 )   Save
    Related Articles | Metrics
    To establish the comparative proteomic technology platform for Demyelinative disease of the central nervous system in cerebrospinal fluid and to screen out disease related protein markers by proteome analysis. MethodsProteins and peptides in CSF were separated by twodimensional gel electrophoresis(2DE) and ultraperformance liquid chromatographer(UPLC) and identified by matrix assisted laser desorption ionization time of flight mass spectrometry（MALDI-TOF-MS）and electrospray ionization(ESI-MS) to study differential proteomic expression in the cerebrospinal fluid(CSF) between patients with Demyelinative disease (the experimental group) and with other neurological diseases (ONDs) (the control group). ResultsDifferent 2DE maps were obtained for the protein spots in the experimental group and the control group gels. Image analysis of silverstained 2DE gels revealed that 10 protein spots showed a significantly differential expression between the two groups of CSF samples. After searching the database, it was found that in the CSF of Demyelinative patients, the expression of hemoglobin was increased. However, the expression of ProstaglandinH2 Disomerase was decreased.Conclusion These identified proteins have potential relations to Demyelinative disease physiology. It would be helpful to find new molecular markers for early diagnosis and treatment of the disease.
    Targeted killing effects of double suicide genes controlled by the MDR1 promoter on C6/ADR cells
    WANG Bo,WANG Zhi-yun,WANG Cheng-wei,WANG Zhi-gang,ZHANG Yuan,ZHANG Qing-lin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  15-18. 
    Abstract ( 1049 )   PDF (506KB) ( 476 )   Save
    Related Articles | Metrics
    Objective To investigate the targeted killing effects of a double suicide gene system controlled by the MDR1 promoter with the prodrug GCV+5-FC on C6/ADR cells. Methods The recombinant vector pcDNA3.MDR1P.CD.TK containing CD and TK double suicide genes controlled by the MDR1 promoter was transfected into C6/ADR cells by using liposome. PCR and RT-PCR were used to identify the integration and expressions of the CD and TK genes. This recombinant vectortransfected C6 cells (C6/CDTK) and normal C6/ADR cells were set as controls. After adding the prodrug, the effects of double suicide genes on cell growth, cell cycle and proliferation were determined by cell growth curve, flow cytometry (FCM) and plate colony formation assay. ResultsPCR proved double suicide genes were integrated into C6/ADR and C6 cells. RT-PCR revealed that the CD and TK genes were expressed in C6/ADR/CDTK cells C6/ADR/CDTK cells was inhibited. G1 phase proportion was significantly higher in C6/ADR/CDTK cells (99.93%) than in C6/ADR cells and C6/CDTK cells (32.68% and 47.57%, P<0.05); the formation rate was significantly lower in C6/ADR/CDTK cells ［(16.7±0.9)%］ than in C6/ADR cells and C6/CDTK cells ［(96.7±2.1)% and (86.7±1.9)%, P<0.05］. ConclusionThe pcDNA3.MDR1P.CDTK/GCV+5FC system has targeted killing effects in C6/ADR cells.
    Effects of immunoglobulin on GABA receptors and expression of GAD in epileptic rats
    YAN Hui-lan,WANG Juan,ZHAO Li-xia
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  19-21. 
    Abstract ( 1170 )   PDF (277KB) ( 538 )   Save
    Related Articles | Metrics
    To study the effects of immunoglobulin on the number of GABA receptors, the expression of GAD and cell apoptosis. Methods Forty healthy SD rats were randomly divided into the blank control group, the epilepsy model control group, the epilepsy model saline (NS) pretreatment group and the epilepsy model immunoglobulin pretreatment group. After being decapitated, the number of GABA receptors, the expression of GAD and cell apoptosis in the brains were determined. ResultsAfter seizures, the number of GABA receptors and the expression of GAD were higher in the model group than those in the control group. After immunoglobulin pretreatment, the number of GABA receptors and the expression of GAD were significantly increased while the number of apoptosis was decreased in the model group. Conclusion Immunoglobulin can control epilepsy by enhancing the expression of GAD and blocking apoptosis.
    Relation between alteration of Cystatin C in cerebrospinal fluid and GuillainBarre′syndrome
    YANG Yin-rong,LIU Shi-lian,LIU Fu-jun,QIN Yan-jiang,BAI Shu-mei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  22-26. 
    Abstract ( 1204 )   PDF (470KB) ( 472 )   Save
    Related Articles | Metrics
    Proteomic analysis was carried out in the cerebrospinal fluid (CSF) of patients with GuillainBarre′syndrome(GBS) and of controls to find the protein presenting the most obvious changes and to investigate its relation with GuillainBarre′syndrome. MethodsCSF was extracted from patients with GBS and controls， and proteins were precipitated with icecold acetone． Isoelectric focusing electrophoresis(IEF) was employed as the first dimension and sodium dodecylsulfatepolyacrylamide gel electrophoresis (SDSPAGE) was employed as the second dimension. The twodimensional analysis gel maps were analyzed by Imagemaster 2D software to find the obvious alteration proteins and attain the proteins in the prepared gels. The peptides mass fingerprint was obtained by MOLDI/TOF/MS, and differential proteins were identified in the SWISSPROT database. ResultsThere were 6 protein spots that had significant differences in expression between the two groups. Expressions of 4 protein spots in the GBS group increased and 2 decreased. Cystatin C which was the most significantly decreased protein was firstly discovered in this study. The subsequent ELISA confirmed that the concentration of Cystatin C was significantly lower in the GBS group than that in the control group［（2.79±0.65）vs（3.65±0.82）mg/L，P＜0.01］. Conclusion That the level of Cystatin C significantly decreases in the CSF of GBS may offer clues for studies on the mechanisms of GBS.
    Expression of CD70 in hepatocellular carcinoma and its suppressive impact on the proliferation of lymphocytes
    WU Han,HAN Zhen-long,CAO Ying-lin,GUO Chun,ZHU Fa-liang,CAO Li-li,ZHANG Li-ning,GAO Shang-xian,SHI Yong-yu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  27-30. 
    Abstract ( 1170 )   PDF (342KB) ( 477 )   Save
    Related Articles | Metrics
    To investigate the expression of the CD70 gene in hepatocellular carcinoma and its role in the immune escape of hepatocellular carcinoma. MethodsCD70 expression was determined by RTPCR. The eukaryotic expression vector（CD70pcDNA3）was constructed and transiently transfected into the HepG2 cell line. After coculturing HepG2 with Jurkat, the effects of CD70 on inhibiting lymphocyte proliferation were assessed by the Cell counting kit 8(CCK8). Results① CD70 gene presented in BEL7402 and SMMC7721 but not in HepG2. CD70 was positive in 3 cases of hepatocellular carcinoma while it was negative in 2 cases of liver tissues adjacent to cancer tissues. ② The eukaryotic expression vector (CD70pcDNA3) was successfully constructed and transiently transfected into the HepG2 cell line. The HepG2 cell line transfected with the CD70 gene can significantly inhibit the proliferation of PHAactivated Jurkat cells. The proliferation inhibition rate of Jurkat cells cocultured with HepG2 cells transfected by the CD70 gene was 65.03%, which was significantly higher than that of Jurkat cells and Jurka cells cocultured with HepG2 cells (38.01% and 30.15%). ConclusionThe CD70 gene is expressed in hepatocellular carcinoma and can inhibit the proliferation of lymphocytes. The CD70 gene may play an important role in the immune escape of hepatoma cells.
    Protective effects of the Huayu Kesai decoction pretreatment on the brains of rats with focal cerebral ischemia and its effect on HSP70 expression
    WANG Yun-man,LI Chang-sheng,YANG Xiao-ni
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  31-35. 
    Abstract ( 1076 )   PDF (594KB) ( 543 )   Save
    Related Articles | Metrics
    To investigate the effects of Huayu Kesai decoction (HYKSD) pretreatment and its related mechanisms on the brains of rats with focal cerebral ischemia. MethodsFocal brain ischemia was induced by the intraluminal suture MCA occlusion method, then the rats were randomly divided into four groups (n=15 per group): the shamoperated group, the ischemia group, the high dose HYKSD pretreatment group（2.34g/mL，1mL/100g）and the low dose HYKSD pretreatment group（1.17g/mL，1mL/100g）. The HYKSD groups were treated with HYKSD for 7 days before MCAO, 1 time per day, whereas the shamoperated group and the ischemia group were given NS. The infarct volume of the rat brains in each group was determined, the neurons were observed under microscopy, and the HSP70 mRNA expression was determined by RTPCR and the HSP70 protein expression by immunohistochemistry 6 hours after ischemia. Results High and low dose HYKSD pretreatment can decrease the damage of neurons and the infarct volume of the brains. The expressions of mRNA and protein were remarkably increased in the ischemia group, and they were remarkably decreased by HYKSD. Conclusion can inhibit the expression of HSP70 mRNA and protein, which may be related to its effect on brain protection against cerebral ischemia.
    Effects of the survivin　antisense oligonucleotide on survivin mRNA expression and chemosensitivity to Paclitaxel in human breast cancer cells MCF-7
    HAN Fang,LI Li
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  36-39. 
    Abstract ( 960 )   PDF (340KB) ( 477 )   Save
    Related Articles | Metrics
    To investigate the effects of the survivin antisense oligonucleotide(ASODN) on cell proliferation，apoptosis and chemosensitivity to paclitaxel in human breast cancer cells MCF-7. MethodsSurvivinASODN was transfected into MCF-7cells mediated by Lip reagent. The expression of survivin mRNA was determined by RT-PCR, cell proliferation and chemosensitivity to paclitaxel in MCF-7 were determined by MTT assay, and apoptosis and cell cycle were determined by flow cytometry. Results The antisense compound efficiently downregulated survivin mRNA expression in a timedependent manner. The growth inhibition rate of MCF-7 was decreased in a dose and timeindependent manner. The cell cycle was arrested at G2/M phase; apoptosis was obviously found. Paclitaxel could enhance the expression of survivin at an early time, and this effect might play a critical role in the escape to apoptosis induced by paclitaxel, which may promote the resistance of paclitaxel. The antisense compound efficiently downregulated this induction by paclitaxel, and enhanced the chemosensitivity to paclitaxel in MCF7. ConclusionSurvivin antisense oligonucleotide effectively suppresses the expression of survivin mRNA, increases apoptosis and enhances the chemosensitivity to paclitaxel in MCF-7.
    Effects of EPA and DHA on the proliferation and apoptosis of glomerular mesangial cells induced by lipopolysaccharide
    GENG Wen-jing,LIU Fang-e,JIAO Bo,CHENG Yan-na
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  37-59. 
    Abstract ( 1042 )   PDF (237KB) ( 364 )   Save
    Related Articles | Metrics
    To observe the effects of EPA and DHA on the proliferation and apoptosis of glomerular mesangial cells(GMCs) induced by lipopolysaccharide(LPS). MethodsThe GMCs were incubed with EPA and DHA at concentrations of 10μmol／L and 100μmol／L for 24, 48 and 72hs. The proliferation of GMCs was determined by MTT. The apoptosis of GMCs was determined by flow cytometry. ResultsBoth EPA and DHA had no effect on the proliferation of normal GMCs. The proliferation of GMCs induced by LPS was significantly inhibited. DHA was more effective than EPA at the same concentration. The apoptosis of GMCs was promoted by EPA and DHA(100μmol／L). ConclusionEPA and DHA could inhibit the proliferation and promote the apoptosis of GMCs induced by LPS.
    Effect of chronic renal failure on nitric oxide synthase gene expression in the penis of rats
    FU Qiang,DING Ke-jia,WANG Fa-cheng,LV Jia-ju,ZHANG Hui
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  40-42. 
    Abstract ( 1079 )   PDF (337KB) ( 398 )   Save
    Related Articles | Metrics
    To investigate the mechanisms of erection dysfunction(ED) induced by chronic renal failure (CRF). MethodsCRF experimental models were established by removing 5／6 kidney from male spraguedawley (SD) rats in one stage. Both the control group (NCRF group, n=6) and the experimental group (CRF group, n=30) were injected with a low dose ［80μg／(kg·d)］ of apomorphine (APO) at the 12th week after resection surgeries to measure the correspondent penile erections. Reverse transcriptase polymerase chain reaction (RTPCR) was used to determine the gene expression of endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) in the rat corpus cavernosum in the 12th week after the resection surgeries. ResultsThe penile erection ratio of the CRF group was 28％ (7／25) while that of the NCRF group was 100％(5／5) (P＜0.05). The average penile erection frequency of the CRF group was(1.0±0.0) while that of the NCRF group was (2.2±0.8) (P＜0.05). The gene expression of nNOS in the rat corpus cavernosum was (0.21±0.07) of the CRF group and (0.40±0.08) of the NCRF group (P＜0.01). The RTPCR results showed that the gene expression of eNOS in the rat corpus cavernosum of the CRF group was significantly lower than that of the NCRF group［(0.28±0.08) vs(0.67±0.13) (P＜0.01)］. ConclusionsCRF significantly reduces the erectile function of the rats, and CRF-related ED has some correlations with the decrease of the gene expressions of eNOS and nNOS in the rat corpus cavernosum.
    Effects of giant knotweed rhizome medicine on the expressions of RAGE and VEGF of rats with diabetic nephropat
    FEI Hong-hua,XIAO Zheng-da,GAO Wei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  43-47. 
    Abstract ( 1101 )   PDF (528KB) ( 413 )   Save
    Related Articles | Metrics
    ObjectiveTo investigate the effects of giant knotweed rhizome medicine on the pathological changes and expressions of receptor for advanced glycation end products (RAGE)、 and vascular endothelial growth factor (VEGF) in nephridial tissues in experimental diabetic rats. Methods36 healthy male Whistar rats were divided into the control group(n=8) and the experimental group (n=28) which were given diets enriched with sucrose(20％w／w), lard(10％ w／w), cholesterol(2.5％ w／w) and cholic acid(0.5％ w／w). After 4 weeks, diabetic rat models were induced by an injection of streptozotocin (STZ) into the vena caudalis and were randomly divided into 3 groups: the model group(fed with distilled water), the giant knotweed rhizome medicine group and the fluvastatin group. Blood glucose(BG), KW／BW(kidney weight／body weigh), creatinine clearance (Ccr), and 24h urinary protein (24 hUP) were determined after 8 weeks. Pathological changes of the kidneys were observed by using light and electron microscopy, and the expressions of receptors for advanced glycation end products(RAGE)and vascular endothelial growth factor(VEGF) in the glomcrulus were determined by using immunohistochemical methods. ResultsAfter interference, compared with the model group, the KW／BW, Ccr, 24?hUP, and the volume of the glomcrulus of the giant knotweed rhizome medicine group were significantly downregulated(P＜0.01 and P＜0.05), also expressions of RAGE and VEGF in the glomcrulus were significantly inhibited(P＜0.05 and P＜0.01). ConclusionGiant knotweed rhizome medicine has a renoprotective effect for diabetic rats, which may relate to the downregulation of expressions of RAGE and VEGF in nephridial tissues.
    Effect of the Tangshen capsule on nephropathy damage in diabetic rats: an experimental study
    ZHANG Xiu-juan,LIU Xian-feng,HE Wen-guang,YANG Jing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  48-51. 
    Abstract ( 1116 )   PDF (368KB) ( 422 )   Save
    Related Articles | Metrics
    To observe the protective effects of the Tangshen capsule on nephropathy damages in diabetic rats and explore the optimal dosage of Astragalus membranaceus in the drug. Methods Diabetic rats were induced by streptozotocin (STZ) and were divided into the diabetic model group（DM）, the highdose treatment group (HDT group), and the lowdose treatment group (LDT group). After an 8week treatment, serum creatinine (Scr), urine albumin (UAlb), sorbitol content in red blood cell (RBCS), fasting blood glucose (FBG), fructosamine (FIM), triglyceride (TG), cholesterol (CHO) and the kidney hypertrophy index were determined. The ultrastructure of the rats′ kidneys was observed after PAS staining. The expression of intercellar adhesion molecule1 (ICAM1) was determined by the immunohistochemical technique. Results① Compared with the DM group, each treatment group had a higher weight, lower Scr, UAlb, RBCS, FBG, FIM and kidney hypertrophy index(P＜0.05); ② Compared with the NC group, the expression of ICAM-1 was upregulated in the other groups. But in treatment groups, the above items were ameliorated(P＜0.05) and they did the best in the HDT group. ConclusionThe Tangshen capsule can offer protection against kidney damage of diabetic rats and the optimal dosage of Astragalus Membranaceus is 120g per powder.
    Role of the unfolded protein response in apoptosis of lens epithelial cells
    YANG Xue-li,CAI Ke-li,GAO Xue,WU Xiao-li,ZHUO Jian,YANG Bo, LIU De-rong,WANG Rong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  52-56. 
    Abstract ( 1144 )   PDF (570KB) ( 485 )   Save
    Related Articles | Metrics
    To investigate the effects of the unfolded protein response(UPR) in endoplasmic reticulum stress on apoptosis of human lens epithelial cells (hLECs) and the relationship between UPR and cataract formation. MethodsCultured hLECs were divided into the control group and stimulated groups A, B, C and D, which were cultured in 1640 with homocysteine(1, 2, 5 and 10mmol／L) for 24h. Inhibition of the cell proliferation was determined by MTT assay; cell apoptosis was determined by Hoechst staining; free glutathione was determined using a Glutathione Quantigication Kit; the levels of cytosolic ROS were determined by adding H2DCFHDA for 20 to 30min followed by imaging with a fluorescent microscope; and the expressions of Bip／GRP78 and Caspase12 in stimulated cells were determined by Western blot. ResultsAfter being exposed to different concentrations of Hcy, LECs showed a dosedependent decrease in cell vitality: 48.2％ declined at 5mmol／L concentration of Hcy and 57.7％ declined at 10mmol／L concentration of Hcy, which showed significant differences compared with the control group (P<001). Also significant apoptotic cells were determined. Compared with the control group, the level of ROS increased in a dosedependent manner and of GSH was decreased in the C and D groups(P<0.05), also the expressions of GRP78 and Caspase-12 were significantly increased in the C and D groups (P<0.05). ConclusionsHigh concentration of endoplasmic reticulum stressors can induce the endoplasmic reticulum and induce apoptosis of LECs through UPR. The conclusion can be drawn that UPR is probably one of the initiating factors of cataracts.
    Effects of antistress on behavior and serum concentrations of IL-6 and cortisol in chronicstressed rats
    FAN Shu-juan,LIU De-xiang,SONG Jing,JIANG Hong,YANG Le-jin,LIU Xin,PAN Fang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  60-63. 
    Abstract ( 1013 )   PDF (295KB) ( 542 )   Save
    Related Articles | Metrics
    To explore the effects of anti-stress on behavior and serum concentrations of Interleukin-6(IL-6) and cortisol in chronic-stressed rats. MethodsThirtytwo healthy Wistar rats were randomly divided into four groups: the control group, the model group, the propranolol group and the phentolamine group, eight in each group. The chronic unpredictable mild stress (CUMS) model was adopted to stimulate depression in humans. Propranolol or phentolamine pretreatment was used as an anti-stress factor. At the end of the experiment, all the rat′s openfield activities were investigated, and serum IL-6 and cortisol concentrations were determined using radioimmunoassays. ResultsOpenfield activities of the model rats had significant depressive changes, with the serum concentrations of IL-6 and cortisol both significantly elevated. Phentolamine pretreatment helped suppress the behavioral changes and cortisol upregulation in stressed rats, whereas there were no obvious effect on elevation of the serum IL-6 levels. Propranolol had no antistress effect. Conclusionα Adrenergic receptor(AR) antagonist pretreatment could protect chronicstressed rats to some extent. The upregulation of stressinduced serum IL-6 may not contribute to the activation of AR in rats.
    Effect of Losartan on the stability of atheromatous plaques in rabbits
    ZHANG Yong-huan,YANG Cai-yun,LI Shi-guo,LI Rui-feng,Dong Bo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  64-67. 
    Abstract ( 1063 )   PDF (529KB) ( 532 )   Save
    Related Articles | Metrics
    To explore the effects of Losartan on the expression of matrix metalloproteinases-3(MMP-3) and the number of macrophages in rabbits with atherosclerotic plaque(AP) and to explore the mechanisms of increasing the stability of AP. MethodsThe experimental atherosclerotic models were induced by endothelial injures on the ventroaorta with a balloon and hyperlipid feeding for four months. Sixteen male New Zealand rabbits were randomly divided into two groups: the model group which was fed with standard diets containing 1％ cholesterol and the Losartan group which was fed with standard diets containing 1％ cholesterol and losartan 25mg／(kg·d). The proteinum expression of MMP-3 and the positive area of macrophage cells in the AP were determined by the immunohistochemistry technique. The structures of the AP of the two groups were observerd with an electron microscope. Realtime RTPCR was used to determine the mRNA expression of MMP-3. ResultsCompared with the model group, the proteinum expression of MMP3 and the number of macrophage cells in the Losartan group were significantly decreased(P＜0.05). The expression of MMP-3 mRNA was more significantly decreased in the Losartan group than that in the model group(P＜0.05). ConclusionLosartan may stabilize vulnerable plaque by suppressing the expression of MMP-3 and reducing the number of macrophage cells.
    Construction and expression of expressional vectors of envelope glycoprotein genes of hantavirus, GM04-38 strain
    WANG ping-hua,TAO Ze-xin,WANG Li-juan,CAO Hai-xia,WANG Zhi-yu,
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  68-71. 
    Abstract ( 1066 )   PDF (335KB) ( 604 )   Save
    Related Articles | Metrics
    To construct expressional vector of glycoprotein G1 and G2 of Hantavirus, GM04-38 strain, express it in Vero E6 cells, and to observe cell fusions. MethodsBy PCR and the gene cloning method, Hantavirus GM0438 strain G1 and G2 genes were cloned into plasmid pCAGGS／MCS. After being confirmed by enzyme digestion and sequence analysis, the pCAGGS-G1 and pCAGGS-G2 were co-transfected into Vero E6 cells. After being treated with acidic MEM, the cells were fixed and stained by Giemsa and were observed under microscopy. IFA was also used to observe the expression efficiencies. ResultsIFA showed the G1 and G2 genes were successfully expressed, and the fluorescent signal was robust and concentrated in the perinuclear region of the transfected cells. Giemsa staining showed that cellcell fusion could be induced under the co-expression of G1 and G2 under acidic conditions, while the coexpression of the NP gene could not enhance the fusion. ConclusionThe expressional vectors of glycoprotein G1 and G2 are successfully constructed and expressed in Vero E6 cells,
    Expression of estrogen receptors α and β in introductal proliferative lesions and invasive carcinoma of the breast: A comparative study
    XU Jia-wen, ZHANG Qing-hui, SUN Ye, ZHOU Cheng-jun, WANG Yan, LIU Wen-jun,ZHOU Zhi-qiang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  72-75. 
    Abstract ( 1310 )   PDF (283KB) ( 524 )   Save
    Related Articles | Metrics
    ObjectiveTo explore the expressions of estrogen receptors α and β in introductal proliferative lesions and invasive carcinoma of the breast. MethodsImmunohistochemistry was used to determine the expressions of ER α and β in 11 cases of usual ductal hyperplasia(UDH), 7 atypical ductal hyperplasia(ADH), 23 ductal carcinomas in situ(DICS)（8 DCISⅠ,11 DCISⅡ,4 DCIS Ⅲ) and 11 invasive breast carcinomas（IBC）. ResultsThe expression level of ERα increased in UDH, ADH and low grade DCIS, while it decreased in high grade DCIS and IBC. The expression of ERα was higher in UDH than in DCIS（P<0.05）. The expression of ERβ decreased in the transformation from normal breast tissues to UDH, ADH, DCIS and IBC, and there were significant differences between normal breast tissues and ADH, DCIS, IBC, as well as among UDH and DCIS, IBC（P<0.05）. No correlation was found between the expressions of ER α and ERβ（P>0.05）. ConclusionsThe expressions of ER α and β are different in the transformation from introductal proliferative lesions to breast carcinoma, and they may act by different mechanisms in carcinogenesis.
    Genetic polymorphisms of CYP17 and risks of cirrhosis or hepatocellular carcinoma in hepatitis B-infected patients
    ZHU Ming-ming,SUN Cheng-gang,LIU Xiao-hua,YANG Ai-xin,SUN Dan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  76-79. 
    Abstract ( 1293 )   PDF (330KB) ( 503 )   Save
    Related Articles | Metrics
    To explore the relationship between genetic polymorphisms of CYP17 MspA1I and cirrhosis or hepatocellular carcinoma in hepatitis B infected patients of Shandong Province. Methods80 healthy controls and 376 HBV patients including 96 hepatocelluar carcinomas(HCC), 107 cirrhosis, 82 hepatitis and 91 HBsAg carriers were determined the T→C in CYP17 MspA1I by polymerase chain reaction and restriction fragment length polymorphism (PCRRFLP). ResultsCompared with the carriers, the (-34) A2/A2 genotype of CYP17 significantly increased the risks of HCC patients. Females mostly contributed to this association (OR＝5.93, P＝0.002), and the OR values increased in postmenopausal women (OR＝6.18, P＝0.003) but not in male subjects. There were no significant differences in the distribution of CYP 17 genotypes between carriers and cirrhosis (P>0.05). ConclusionCYP17 MspA1I genotypes A2/A2 may be related to liver cancer risk in HBVinfected women in Shandong Province.
    Calcium dobesilate in the treatment of early diabetic nephropathy
    DONG Jing,LIANG Cui-ge,ZHANG Xiao-qian,LI Ming-long,LIU Zhan-feng,ZHAO Jia-jun,YU Gui-na
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  80-83. 
    Abstract ( 1277 )   PDF (263KB) ( 485 )   Save
    Related Articles | Metrics
    To investigate the clinical effects of calcium dobesilate on early diabetic nephropathy. Methods42 patients with early diabetic nephropathy were randomly divided into the treatment and control groups. All the patients were given treatment to control blood glucose and 21 patients in the treatment group were treated with calcium dobesilate (500?mg, three times per day) for 12 weeks. Urinary albumin excretion, plasma endothelin, serum nitric oxide, serum glucose, glycosylated heamoglobin A1c, hepatic and renal function were determined before and after the treatment. ResultsAfter 12 weeks of treatment, in the treatment group the levels of UAE were significantly decreased（P＜0.01), the levels of ET were decreased, the levels of NO were increased, and the levels of serum glucose and HbA1c had no changes. However, the levels of UAE, ET, NO, serum glucose and HbA1c had no changes in the control group. ConclusionCalcium dobesilate can decrease the levels of UAE in patients with early diabetic nephropathy and postpone the development of diabetic nephropathy, which may be due to its correction of abnormal renal hemodynamics through decreasing ET and increasing NO.
    Clinical effect of concurrent chemoradiotherapy foradvanced esophageal cancer
    FU Wei-jiang,YANG Qiu-an,HAN Wen-fei,FU Lei,GAO Shuang,PAN Zhen-hua,CHENG Xiao-guo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  84-87. 
    Abstract ( 1125 )   PDF (279KB) ( 480 )   Save
    Related Articles | Metrics
    To explore the effects, toxicity and safety of concurrent chemoradiotherapy for esophageal cancer, which remains controversial, especially for advanced patients. Methods A total of 75 patients with esophageal cancer having T4 tumor or N1 regional lymph node invasion or distant lymph node metastasis(M1a) were enrolled in this study. FT207 800mg/d and CDDP 20mg/d were administered on days 1-5 and 22-26. Fractionated radiotherapy was performed from day 1 and a total dose of 60-70Gy was delivered at the rate of 2Gy per fraction. A t-test was used to compare the size of lesions before and after therapies. The overall survival time was determined by the KaplanMeier method. Results The overall response rate was 64% and the complete response rate was 14.7%. The size of lesions that could be determined in two directions was (10.53±2.87)cm2 before therapies and (4.69±1.25)cm2 after therapies　(P=0.012). The size of lesions that could be measured only in one direction was (7.86±2.01)cm before therapies and (3.83±1.08)cm after therapies (P=0.007). The toxicity of the most common grade 3 was endoesophagitis(8%) and nausea and vomiting (21.3%) and of grade 4 was not observed. The 2-year survival rate was 30.7%. Conclusion Based on the overall response rate and the toxicity of treatments, the results obtained from the present trial are promising. Concurrent chemoradiotherapy is currently suitable for patients with advanced esophageal cancer.
    Effect of conventional and laparoscopic surgery on peritoneal fibrinolytic capacity： a prospective randomized clinical trial
    HU Xing-qian,ZHAO Han-lin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  88-90. 
    Abstract ( 962 )   PDF (244KB) ( 413 )   Save
    Related Articles | Metrics
    To investigate the fibrinolytic changes in peritoneal biopsies during laparoscopic and conventional abdominal surgeries. MethodsIn laparotomies, peritoneal biopsies were taken when the abdomen was opened and just before being closed. In laparoscopies, peritoneal biopsies were opened after carbon dioxide insufflation and were closed just before exsufflation. Concentrations of tissuetype plasminogen activator (tPA), plasminogen activator inhibitor type 1 (PAI-1) and the resulting tPA activity were determined. ResultsConcentrations of tPA in peritoneal tissues decreased during operations in both groups, but was more significantly in the laparotomy group (P<0.05). PAI-1 levels in opening biopsies were higher in the laparoscopy group than that in the laparotomy group (P<0.05). PAI-1 concentrations were also increased in laparotomies but not in laparoscopies. At the end of the operations, there were no differences between the two groups. The resulting tPA activity did not differ between the two groups at opening or closure, but it was decreased in both groups during operations (P<0.05). ConclusionThese findings indicate that the peritoneal response to open and laparoscopic surgeries is similar. The initial rise in peritoneal PAI-1 concentration during laparoscopies suggests adverse effects of carbon dioxide insufflation, which might affect peritoneal repair.
    Correlation between coronary artery plaque and acutecoronary syndrome by MSCT
    GAN Jie,LIU Ping,WANG Ying,ZHU Jun,pU Chun-qing,LI Da-hua,WEI Wei-wei,LI Bin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  91-93. 
    Abstract ( 1039 )   PDF (334KB) ( 585 )   Save
    Related Articles | Metrics
    To evaluate the correlation between coronary artery plaque and acute coronary syndrome (ACS) by multislices spiral computed tomography (MSCT). Methods104 patients suspected of coronary heart disease were subjected to MSCT. The results were compared with the clinical appearance. ResultsFatty plaque had correlations with ACS (P<0.05), but calcified and fibre plaque had no close correlations with ACS (P>0.05). ConclusionMSCT is considered suitable for evaluation of the correlations between coronary artery plaque and ACS.
    Proportional changes of HBeAg negative chronic hepatitis B and liver cirrhosis B and the HBV genotype in Shandong Province
    LIU You-de,WANG Lei,ZHOU Pei-jing,ZHUANG Hui
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  94-96. 
    Abstract ( 1001 )   PDF (238KB) ( 584 )   Save
    Related Articles | Metrics
    To investigate the proportional changes of HBeAg negative chronic hepatitis B(CHB) and liver cirrhosis of hepatitis B (LC-B) and the HBV genotype in Shandong Province. MethodsThe clinical data of 2?561 hospitalized cases of CHB and LC-B from 1996, 2001 and 2006 were retrospectively analyzed. The HBV genotypes were determined in 220 patients with CHB or LC-B by using the nested polymerase chain reaction based on genotype specific primers. ResultsThe proportion of HBeAg-negative LC-B(50.0%) was higher than that of CHB(23.8%), P＝0.000. It rose from 46.1% in 1996 to 54.4% in 2006, P＝0.047. The percentages of genotype C and B/C mixed genotypes in the HBeAg negative CHB and LC-B groups were 94.7%, 5.3% and 90.0% 10.0% respectively, which were not statistically different from the percentage in the HBeAg positive group. No genotype B or other genotypes were found. ConclusionsIn Shandong Province, HBeA positive is still the predominant form of hospitalized CHB, the proportion of HBeAgnegative LCB has been recently more than 50%. Genotype C is the predominant genotype in both HBeAg negative and positive patients. No genotype B is found in HBeAg negative patients.
    Clinical trial of human albumin microspheres，injectable suspension octfluoropropane formulation for improvement ofendocardial border delineation
    WANG Zhi-yun, CHEN Liang, ZHANG Mei,JIANG Gui-hua,YAO Gui-hua,BU Pei-li,JI Xiao-ping,ZHANG Yun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  97-99. 
    Abstract ( 1319 )   PDF (262KB) ( 411 )   Save
    Related Articles | Metrics
    To explore the safety and efficacy of human albumin microspheres，injectable suspension octfluoropropane formulation for left ventricular endocardial border delineation（LVEBD）. MethodsFortyfive patients with suboptimal echocardiography were enrolled in this study. Each patient received 0.01ml/kg human albumin microspheres，injectable suspension octfluoropropane formulation. Twelve segments in apical fourand twochamber views were scored for LVEBD. ResultsLVEBD was significantly improved in 96.9% segments. There were no significant changes of electrocardiogram， heart rates， blood pressure，respiratory，blood routine，urine routine，liver function and kidney function observed after treatments. ConclusionsAlbumin microspheres，injectable suspension octfluoropropane formulation is safe and valuable for LVEBD assessment.
    Correlation between arterial stiffness and acute coronary syndrome in aged patients
    ZHANG Zhi-mian,YANG Xiao-yun,LIANG Ji-le,DAI Jun,GAO Xi-mei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  100-103. 
    Abstract ( 1317 )   PDF (261KB) ( 514 )   Save
    Related Articles | Metrics
    To explore the changes of arterial stiffness and their relative factors in aged patients with acute coronary syndrome (ACS). Methods308 aged patients with ACS, aged 62 to 91 years (average 66.78±9.70 years, 203 males, and 105 females) and 138 aged subjects with normal coronary arteries, aged 61 to 89 (average 64.57±9.37 years, 86 males, and 52 females) were enrolled in this study. Brachial systolic and diastolic blood pressures were determined. ResultsCompared with the control group, the PWV value was significantly increased in the ACS patients（12.16±2.08 vs 10.21±2.36, P＜0.01）, and the systolic blood pressure and pulse pressure were significantly higher in the ACS patients (both P＜0.01). ConclusionArterial stiffness is significantly increased in patients with ACS, and SBP and PP are closely related to arterial stiffness.
    Remifentanil combined with lornoxicam make extubation comfortable and painless during recovery fromgeneral anesthesia
    ZHANG Zhao-hang,DU Hong-mei,ZHANG Li,LI Dong-liang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(1):  104-107. 
    Abstract ( 1172 )   PDF (288KB) ( 418 )   Save
    Related Articles | Metrics
    To study the efficacy of remifentanil or/and lornoxicam in reducing the adverse effects during recovery from general anesthesia. MethodsSixty ASA Ⅰ to Ⅱ patients aged 18 to 60 years who experienced oral or maxillofacial operations, were divided into four groups(n=15): group Ⅰ (remifentanil infusion was stopped at the end of the operations), group Ⅱ(remifentanil infusion turned into 0.05μg/(kg·min) after the completion of the operations and remained till extubation), group Ⅲ (8 mg lornoxicam was intravenously administered at 20 min before introduction and 30 min before the completion of the operations), and group Ⅳ( remifentanil was used as group Ⅱ and lornoxicam was used as group Ⅲ). Anesthetic induction and maintenance were the same in the four groups. The rate of restlessness and cough during recovery, the time of opening eyes when called, the time of extubation, blood pressure (BP), heart rate (HR) at completion of the operations, extubation, and 5 and 10min after extubation, and the scores of verbal rating scale (VRS) at 5 and 10 min after extubation were determined. ResultsThe cough rate in groups Ⅱ and Ⅳ was lower than that in group Ⅰ（P＜0.05）， the times of opening eyes and of extubation were also longer than those in group Ⅰ（P＜0.05）. The BP and HR in groups Ⅱ, Ⅲ and Ⅳ were significantly lower than those in group Ⅰ at extubation and 5 min after extubation （P＜0.05 or P＜0.01）, also they were lower in groups Ⅲ and than in groups Ⅰ at 10 min after extubation (P＜0.05 or P＜0.01）, but they were steady in group Ⅳ during the recovery. The VRS scores were remarkably lower in group Ⅱ, Ⅲ and Ⅳ than in group Ⅰ at 5 min after extubation（P＜0.05）and were significantly lower in group Ⅲ and Ⅳ than in group Ⅰ and Ⅱ at 10 min after extubation（P＜0.05）. ConclusionAdministered remifentanil or lornoxicam can reduce adverse effects during recovery from general anesthesia, and their combination makes extubation comfortable, painless and safe.
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd