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Table of Content

      
    16 February 2008
    Volume 46 Issue 2
    Articles
    Spatial semivariogram models of human population genetic structure
    SHAO Qi,XUE Fu-zhong,WANG Jie-zhen,CHENG Yu,ZHANG Gui-qin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  111-114. 
    Abstract ( 1084 )   PDF (393KB) ( 675 )   Save
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    To establish a spatial semivariogram model of human population genetic structure. MethodsSpatial semivariogram models were set up based on genetic distance by analyzing the relationship between geographic distance and genetic distance. Results The spatial semivariogram model revealed the spatial genetic structures of a human population. Conclusion Spatial semivariogram models of human population genetic structure can effectively reveal the spatial structure of the population genetic structure.
    Construction of the luciferase reporter plasmid containing human Tim-3 promoter
    ZHANG Hong-kun,JU Ying,LUAN Fang,GUO Chun,MA Chun-hong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  115-118. 
    Abstract ( 1088 )   PDF (486KB) ( 456 )   Save
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    To construct a luciferase reporter plasmid containing human T-cell immunoglobulin- and mucin-domain-containing molecule-3(Tim-3) gene promoter. Methods The human Tim-3P2 was amplified by PCR using human genomic DNA as a template and was directionally cloned into pGL3basic multiple cloning sites to construct the luciferase reporter plasmid pGL3-Tim-3P2. To detect the transcriptional activity of human Tim-3P2 in the plasmid, transient transfection was performed in different cell lines. pRL-TK was used to normalize the transfection efficiency. Results DNA sequencing verified the successful construction of the plasmid pGL3-Tim-3P2. The results of transient transfection showed that the recombinant plasmid could be lowly expressed in the murine macrophage cell line RAW264.7 and murine melanoma cell-line-B16, both of which can endogenously express Tim-3, but not in the African green monkey kidney cell line COS-7 in which no endogenous Tim-3 could be detected. ConclusionThe recombinant plasmid containing human Tim3P2 has been successfully constructed which could selectively express in Tim-3 positive cells.
    Recombinational expression of classical pathway secreted human IL-1β in hepatoma H7402 cells
    XIAO Wei-ling,LIN Ya-jie,MU Dong-zhen,SUN Ping,LIANG Shu-juan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  119-122. 
    Abstract ( 1032 )   PDF (389KB) ( 646 )   Save
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    To construct a classical pathway expressing secretory human interleukin 1 beta (shIL-1β) recombinant vector and to analyze its expression level in H7402 hepatoma cells. MethodsThe total RNA was isolated from LPS stimulated healthy donor PBMCs, and the full length human interleukin 1 beta(hIL-1β) gene was obtained by RTPCR. The human EPO signal peptide gene was prepared by primer overlapping extension, and fusion gene encoding both the EPO signal peptide and mature IL-1β protein was synthesized through gene splicing by over lap extension(SOE). The product was directly cloned into pIRES2EGFP to construct the recombinant eukaryotic expression vector pIRES2EGFPshIL-1β which was verified by PCR, restriction enzyme assay(BamH I and EcoR I) and DNA sequencing. Purified pIRES2EGFPshIL-1β was stably transfected into the H7402 hepatoma cells by jetPEI, then the expression of the recombinant vector was verified under fluorescence microscopy, and the level of fusion gene mRNA was determined by RTPCR and of hIL-1β in cellular supernatant was assessed by ELISA. ResultsThe expression vector pIRES2EGFPshIL-1β that could induce the expression of hIL-1β was successfully prepared through the conventional protein secretory pathway. After the recombinant vector was transfected and selected with G418, the human H7402/shIL-1β cells could effectively express the fusion gene mRNA, and the active hIL-1β in the cellular supernatant was also significantly increased compared with the H7402/mock cells. ConclusionThe recombinant pIRES2EGFPshIL-1β vector that could express hIL-1β was successfully established through the conventional protein secretory pathway.
    Expression of p38 mitogen-activated protein kinase in early embryos and the peri-implantation endometrium in mice
    YU Qing-mei,WU Yu-ling,SONG Hai-yan,YIN Hua-wei,ZHUANG Yuan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  123-127. 
    Abstract ( 1146 )   PDF (517KB) ( 589 )   Save
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    To explore the expression of p38 mitogenactivated protein kinase(p38MAPK)in early embryos and the endometrium during periimplantation in mice. MethodsEarly embryos at different stages and uterus of the non-pregnant, and pregnant uterus of 1 to 8 days pseudopregnancy in mice were obtained. The expression and changes of p38MAPK in early embryos and the peri-implantation mouse endometrium were examined by immunohistochemistry. An image analysis was also performed. Results① p38MAPKpositive staining was found in the preimplanted embryos and gradually increased with development. ② In the pregnant group, p38MAPKpositive expression was weak at 3 days and peaked at 4 to 5 days in the endometrium of pregnant mice, then declined in the lumina epithelium, but increased in decidual cells. In the pseudopregnant group ,weak staining of p38MAPK was only observed on the 4th day of gestation, and negative staining was observed on the other days. p38MAPK was expressed weakly in the endometrium of non-pregnant mice. Conclusion① p38MAPK activity may be required to support embryo development through the murine pre-implantation interval. ② p38MAPK, in addition to relating to blastocyst implantation,may indirectly participate in the process of endometrial decidualization. ③ The expression of p38MAPK in mouse endometrium is regulated not only by maternal factors (steroid hormones), but also by the implanted embryos.
    Cloning, expression and purification of human spermidine/spermine N1acetyltransferase gene
    SUN Hui,GONG Lei,LIU Bin,YANG Ya-pei,XU Chun-xiao,LIU Xian-xi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  128-131. 
    Abstract ( 1152 )   PDF (380KB) ( 690 )   Save
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    To construct the prokaryotic expression plasmid of human spermidine/spermine N1-acetyltransferase (SSAT) and to induce and purify the recombined SSAT protein in E.coli JM109 (DE3). MethodsThe total RNA was extracted from colon cancer tissues and a 513bp cDNA was amplified by RT-PCR with two primers, which span the coding region of SSAT. The synthesized cDNA was subcloned into the prokaryotic expression vector pTriEx-4. The constructed vector pTriEx-4-SSAT was transformed into competence E. coli JM109 (DE3) and then was induced by IPTG. The protein was verified by SDSPAGE and Western blot with the anti HisTag monoclonal antibody. The fusion protein was purified by the Ni-NTA chromatographic column. ResultsThe prokaryotic expression plasmid pTriEx-4-SSAT was successfully constructed, and identified by digestion and sequence. An approximate 20kD exogenous protein was observed on the SDS-PAGE. The protein was verified by Western blot with an anti His-Tag monoclonal antibody. The fusion protein including 6His-Tag was purified by the NiNTA chromatographic column. ConclusionThe SSAT prokaryote expression vector was successfully constructed and the purified protein can be applied for further research of the immunity of SSAT.
    Package and expression of lentivirus encoding hTERT gene
    CUI Li-ping,CUI Jing,SHI Chang-wen,SUN Qing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  132-135. 
    Abstract ( 1230 )   PDF (352KB) ( 519 )   Save
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    To construct a lentiviral vector encoding human telomerase reverse transcriptase(hTERT)genes. MethodsA fragment of the hTERT gene was amplified by RT-PCR and was inserted into the plasmid L166. The three plasmids L166hTERT, L205 and L311 co-transfected the virus packaging cell line 293T using the CaCl2 method. Then the cell supernatant was harvested and virus titration was determined. Also the expression of hTERT in 293T cells infected by the lentivirus was determined by using the immunocytochemistry method. Results DNA sequencing demonstrated that the recombinant plasmid L166hTERT was successfully constructed. The titration of the un-concentrated lentivirus was 5.25×106IU/mL. hTERT polypeptid immunocytochemistry staining proved positive for the hTERT gene in infected 293T cells. Conclusion Lentivirus encoding hTERT was successfully packaged and hTERT could express in 293T cells for more than 2 months.
    Effects of P2X receptors within the nucleus ambiguous on hypoxian respiration in rabbits
    ZHANG Hui,ZHANG Ming-hua,LI Qin,CHEN Ting-shun,ZHAO Qi,WANG Hui,YU Shu-yan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  136-139. 
    Abstract ( 1191 )   PDF (420KB) ( 433 )   Save
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    To investigate the effects of P2X receptor in the nucleus ambiguous (NA) on acute hypoxic ventilation. Methods Experiments were performed on anesthetized, immobilized and artificially ventilated rabbits. The peripheral chemo-reflex to hypoxia was elicited by ventilating the animals with 10%O2balance 90%N2. Changes in the peak amplitude and frequency of integrated phrenic nerve discharge were recorded. The ventilatory responses of the peripheral chemoreflex following microinjection within the NA of either P2X receptor agonist adenosine triphosphate (ATP) or antagonist pyridoxal-5′-phosphate-6-azo-phenyl-2, 4-disulfonate (PPADS) were observed. ResultsExcitation of the phrenic never discharge was induced by ventilating the animals with hypoxic mixed gases. Microinjection of ATP in NA increased the frequency of acute hypoxic respiration, whereas microinjection of PPADS partially blocked the respiratory effects of ATP microinjected into NA. ConclusionThe present results suggested that ATP acting via the P2X receptor in the NA may be involved in the respiration control of the acute hypoxic ventilatory response.
    Effect of Captopril on myocardium gap junction remodeling during tachycardia-induced cardiomyopathy
    TAO Wen,ZHONG Jing-quan,ZHANG Wei,LI Yan,HOU Xue-mei,ZHANG Yun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  140-144. 
    Abstract ( 1044 )   PDF (534KB) ( 486 )   Save
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    To investigate the effects of Captopril on left ventricular connexin remodeling during tachycardiainduced cardiomyopathy in canines. Methods Twenty-four dogs were randomly divided into 3 groups. In the pacing group, each dog was implanted with a permanent pacemaker (pacing at 350-450 bpm), the pacing electrode was implanted in the right atrial appendage through the external jugular vein. In the pacing and drug group, each dog was given Captopril 50mg twice a day three days before the pacemaker was implanted and through out the study. The pacing procedure was the same as in the pacing group. In the control group, no pacemakers or drugs were given. Before pacing and 1,4,8 weeks after pacing, the echocardiography and ECG were measured, and also the heart function was measured. After 8 weeks′ pacing, catheterization was used to assess the systolic and diastolic functions and the content of Cx43 was determined by confocal immunofluoroscopy. ResultsThe content of Cx43 was significantly different between the pacing group and the control group(P<0.001)and between the pacing group and the pacing and drug group(P<0.05). Conclusions Captopril may prevent the decrease of the content of Cx43, and prevents gap junction remodeling in dogs with long-term rapid atrial pacing.
    Brain expression of matrix metalloproteinase-9 in focal cerebral is chemia of diabetic rat and its relationship with the vascular endothelial growth factor
    SHANGGUAN Shi-na,ZHU Mei-jia,JIA Qian,BI Xi-zhuang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  145-148. 
    Abstract ( 1183 )   PDF (402KB) ( 635 )   Save
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    To investigate the involvement of matrix metalloproteinase-9 and the vascular endothelial growth factor in diabetic rat brains during different reperfusion times after focal cerebral ischemia. Methods 120 Wistar rats were randomly divided into 4 groups: the normal control group, the operation control group, the simple cerebral ischemic rats(CI group) and the diabetic cerebral ischemic rats(DM+CI group). Rat models of diabetes mellitus were made by high sucrose, fat diet and streptozotion injection. The models of the middle cerebral artery occlusion were made by an improved zealong′s method. The expressions of MMP-9 and VEGF in the brain tissues were determined by immunohistochemistry staining. ResultsMMP-9 and VEGF mainly distributed in the cytoplasm of neuroglia cells,neurons, some vascular endothelial cells, and in the ischemic penumbra zones of the DM+CI group and the CI group. Along with the reperfusion times,the expressions of the cells changed differently. MMP-9 and VEGF significantly increased in the DM+CI group and the CI group compared with the normal control group (P<0.05).MMP-9 was more significantly increased in the DM+CI group than in the CI group (P<0.05) and peaked at 24h; VEGF was more significantly decreased in the DM+CI group than in the CI group (P<0.01) and peaked at 6?h. There was a linear correlation between MMP-9 and VEGF expressions in the DM+CI group and the CI group. In the DM+CI group, MMP-9 and VEGF expressions were negatively correlated at 6-24h after reperfusion. ConclusionsThe increase of MMP-9 expression contributes to the decrease of VEGF and it may participate in the pathology of reperfusion of diabetic infarct brains.
    Effect of cytokines on the trans-differentiation of fibroblasts in pulmonary fibrosis
    申玉瑶,王建平,王永康,窦建明
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  149-153. 
    Abstract ( 1286 )   PDF (595KB) ( 482 )   Save
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    To investigate the TGF-β1, PDGF-AA, PDGF-BB and α-SMA expressions at different times on rats with pulmonary fibrosis (PF). MethodThe expressions of TGF-β1, PDGF-AA, PDGF-BB and α-SMA were determined by immunohistochemical methods. ResultsAfter bleomycin treatment, Fb showed the most positive reaction to anti-α-SMA on the 7th day, and then it significantly decreased. The expression of TGF-β1 continuously increased in the early and middle stages after bleomycin treatment (7-14 days), but decreased in the late stage. The expression of PDGF-AA in Fb and AM was the highest on the 7th day, and then it gradually decreased. The positive staining for PDGF-BB in Fb was similar to that of PDGF-AA, but AM showed a strong positive staining on the 14th day. ConclusionTGF-β1, PDGF-AA and PDGF-BB deriving from autocrine by Fb play primary roles on the trans-differentiation of fibroblasts. AM is an important source of TGF-β1 and PDGF.
    Effect of FLIP-siRNA-mediated gene silencing on bionomics and growth inhibition in the ovarian cancer cell line A2780
    WANG Yu,LIU Pei-shu,MAO Hong-luan,ZHOU Li-guang,SUN Yu-lan,JIANG Hong,WANG Rong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  154-158. 
    Abstract ( 1191 )   PDF (386KB) ( 400 )   Save
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    To investigate the effects of FLIP-siRNA-mediated gene silencing on bionomics and growth inhibition in the ovarian cancer cell line A2780. Methods Based on the sequence of FLIP mRNA, three siRNAs were designed and chemically synthesized. They were transfected into the ovarian cancer cell line A2780 with LipofectamineTM2000. The FLIP mRNA and protein levels were determined by reverse transcriptionpolymerase chain reaction (RT-PCR) and Western blot. The FLIP-siRNA having the most powerful inhibition was selected. Cell growth inhibition and apoptosis were determined by MTT assay and flow cytometry. Results Short siRNAs targeting FLIP down-regulated the mRNA and the protein level of the FLIP oncogene(P<0.01), and the inhibition rate reached to 77.4% and 66.1%, respectively. The proliferation of the ovarian cancer cell line A2780 was inhibited(P<0.05)and the ratio of apoptosis significantly increased after transfection(P<0.05). Conclusion FLIP-siRNAs can significantly downregulate FLIP expression in transcriptional and translational levels, inhibit cell growth and induce cell apoptosis in the ovarian cancer cell line A2780.
    In vitro differentiation of rabbit bone marrow derived mesenchymal stem cells into chondrocytes
    LI Zhong-hua,LUAN Bao-hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  159-162. 
    Abstract ( 1072 )   PDF (508KB) ( 502 )   Save
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    To explore chondrogenic differentiation of rabbit bone marrow derived mesenchymal stem cells(BMSCs)in experimental culture medium. Methods BMSCs, isolated from rabbit bone marrow aspirates by density gradient centrifugation,were cultured, expanded and induced into chondrocytes in defined culture medium containing bone morphogenetic protein(BMP)-2 in monolayer culture in vitro. Toliudine blue staining, Masson staining, immunohistochemical staining, and Alcian blue chromatomery of glycosaminoglycan (GAG) were used to determine the differentiation. Statistical analysis of the proliferation and chondrogenic differentiation abilities of the induced BMSCs was deployed among the 2nd, 4th and 7th passages. ResultsInduced BMSCs had characteristics of the chondrocyte. There was no statistical difference in the proliferation and chondrogenic differentiation abilities between the P2 and P4 BMSCs (P>0.05). Compared with the P2 and P4 BMSCs, the proliferation and chondrogenic differentiation abilities of the P7 BMSCs was decreased(P<0.01). Conclusion BMP-2 can induce BMSCs to differentiate into chondrocytes in monolayer culture in vitro. Cell passage is an important factor in the chondrogenesis differentiation of BMSCs.
    Effects of human amniotic homogenate on blebs and expressions of TGFβ1 and CTGF after experimental trabeculectomies in rabbits
    GAO Ji-yun, ZHANG Wei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  163-166. 
    Abstract ( 1036 )   PDF (402KB) ( 560 )   Save
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    To investigate the effects of human amniotic homogenate on blebs and the expressions of TGFβ1 and CTGF following trabeculectomy in rabbits. MethodsThirty New Zealand white rabbits were randomly divided into two groups: the control group and the experimental group. The right eyes were subjected to standard trabeculectomies. After the operations, human amniotic homogenate was topically used in the experimental group four times daily for 14 days and was not used in the control group. The clinical features and the pathological changes were observed on different days after surgeries. Results14 eyes in the experimental group had functional blebs and 8 in the control group(P<0.05). The intraocular pressure of the control group was higher than that of the experimental group after 2 days(P<0.05). The fibroblasts in the experimental group were less than those in the control group(P<0.05). The expressions of TGFβ1 and CTGF were lower in the experimental group than those in the control group(P<0.05). Conclusions The supernatant of a homogenized amnion has no side effects, and it can inhibit the expressions of TGFβ1 and CTGF and improves the filtering function and decreases the intraocular pressure.
    Coping styles, emotion regulation and posttraumatic stress response in medical students
    DU Juan,ZHANG Hong-jing,LI Yun,NIU Juan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  167-170. 
    Abstract ( 1294 )   PDF (284KB) ( 554 )   Save
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    To explore the correlations between post-traumatic stress response and coping styles and emotion regulation in medical students. Methods 405 college students accepted examinations with Symptom Selfrating Scale of Posttraumatic Stress Response, Emotion Regulation Questionnaire, Coping Styles Questionnaire and Positive and Negative Affects(PANAS). Based on the self-rating scale standard, they were divided into the symptom group and the control group. Results Compared with the control group, the symptom group had more avoidance and suppression in positive emotion, and also had more rumination in negative emotion(P<0.001 or P<0.05), while they had less positive emotion than the control group. In the coping styles, the symptom group preferred fancy, selfreproach, keeping out of the way and rationalization. The posttraumatic stress responses were positively correlated with avoidance in positive emotion, rumination in negative emotion, and self-reproach(P<0.01),  while negatively correlated with suppression in negative emotion, problem solving and asking for help(P< 0.05 or P< 0.01). Stepwise linear regression showed that rumination in negative emotion, frequency of experiencing negative emotion and fancy were independently predictive variables of posttraumatic stress responses. ConclusionsThere are close relationships between the posttraumatic stress response and inappropriately coping styles and emotion regulation.
    Relationship between hepatitis C virus quasi-species complexity and its response to interferon therapy
    ZHANG Xiao-guo,WANG Min,CHEN Jian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  171-173. 
    Abstract ( 999 )   PDF (297KB) ( 548 )   Save
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    To investigate the relationship between hepatitis C virus(HCV)hypervariable region 1(HVR1) quasispecies complexity and the response to interferon (IFN) therapy. MethodsThe types of HCV were determined by the gene chip technique and the HVR1 quasispecies complexity was determined by the polymerase chain reactionmediated single strand conformation polymorphism (SSCP) method. ResultsAfter IFN treatment,the HCV RNA negative transformation rates in SSCP bands of HCV HVR1≤3 and >3(detected prior to the IFN treatment) were 77.78% and 21.43%, and there were significant differences between them(P<0.01). The number of SSCP bands was 2.44±0.73 in the effective group while it was 4.50±0.65 in the ineffective group, also there were significant differences between them(P<0.01). ConclusionThe high complexity of HCV HVR1 quasispecies indicates nonresponse to IFN therapy in patients with HCV 1 b infection, and it is an important predictive factor for the effectiveness of IFN therapy.
    Expressions and significance of p53 proteins in simple intestinal metaplasia, atypical intestinal
    ZHAO Zhu-mei,ZHANG Jian-ping,YANG Jing-yan,LIU Wen-jun,WANG Yan,ZHOU Zhi-qiang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  178-180. 
    Abstract ( 1162 )   PDF (529KB) ( 476 )   Save
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    To study the expressions of p53 in simple intestinal metaplasia(SIM), atypical intestinal metaplasia(AIM)and gastric adenocarcinoma. MethodsThe immunohistochemical EnVision method was used to determine the expressions of p53 in simple intestinal metaplasia(SIM), atypical intestinal metaplasia(AIM)and gastric adenocarcinoma. ResultsThe positive rates of p53 in SIM, AIM and gastric adenocarcinoma was 13.6%, 40.0% and 55.0%. The expression of p53 in AIM was significantly higher than that in SIM. There was no significant differences between AIM and gastric adenocarcinoma. Conclusionp53 may play an important role in intestinal meteplasia and gastric carcinoma.
    Relationship between the -420C>G polymorphism of the resistin and cerebral infarction
    YANG Li,JI Zhong-guo
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  181-184. 
    Abstract ( 1044 )   PDF (304KB) ( 472 )   Save
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    To investigate the correlation between the -420C>G polymorphism of the resistin gene and cerebral infarction. MethodsThe single nucleotide polymorphism (SNP) of resistin was demonstrated by means of PCR and digested by restriction endonucleases in 176 cases of cerebral infarction and 140 healthy controls. The study group was subdivided into two groups: the cerebral thrombosis group(94 cases) and the cerebral embolism group(82 cases). ResultsThere were three genotypes at the site of -420 of resistin gene: CC, CG and GG. Also there were significant differences of the CC/(CG+GG) genotype(P=0.016?2) and the C/G allelic frequencies(P=0.014?3) between patients with cerebral thrombosis and healthy subjects. Subjects with cerebral infarction showed a significant association between cardiovascular diseases and SNP-420C>G(P=0.014). The CG+GG genotype had a high frequency. There were no associations between type 2 diabetes and insulin resistance or SNP-420C>G. ConclusionSNP-420C>G of the resistin gene may play a role in cerebral infarction and cardiovascular diseases probably by accelerating atherosclerosis.
    The relationships between first febrile convulsions and herpesvirus 6 infection
    ZHANG Dong-qing,SUN Ruo-peng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  185-187. 
    Abstract ( 1151 )   PDF (239KB) ( 352 )   Save
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    To explore the relationships between the first febrile convulsions and herpesvirus 6 (HHV-6) infection. Methods HHV-6 infection was determined by PCR in 75 children. The clinical characteristics of the first febrile convulsion were compared between those with and without HHV-6 infection. Results HHV-6 infection was found in 26 of 75 patients with the first febrile convulsions. The median age of patients with the first febrile convulsion and HHV-6 infection was significantly lower than those without HHV-6 infection(P<0.01). The frequency of partial seizures, clustering seizures, long lasting seizures, and postictal paralysis was significantly higher among those with primary HHV-6 infection than that among those without(P<0.05). The frequency of the first febrile convulsions in infants younger than 1 year and with HHV6 infection was also significantly higher than those without HHV6 infection(P<0.05). Conclusions HHV-6 infection has a close relationship with febrile convulsions in infants and young children and it often induces more severe convulsions and might be a risk factor for subsequent development of epilepsy.
    The QRS interval for cardiac insufficiency following acute myocardial infarction
    MIAO Jun-dong,LIU Wei-hua,REN Man-yi,SUI Shu-jian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  188-191. 
    Abstract ( 1444 )   PDF (275KB) ( 857 )   Save
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    To explore the role of the QRS interval for cardiac insufficiency in the immediate future following acute myocardial infarction (AMI). Methods120 patients with AMI for the first time were enrolled in the study and divided into four groups based on the heart function classification of Killip recorded within 72 hours after hospitalization. The density of brain natriuretic peptide (BNP) was determined in the plasma and the QRS interval was determined by electrocardiography. All patients were reviewed and followed up for 6 weeks and at the 6th week, the LVEF was determined by echocardiography. ResultThe QRS interval and level of BNP were increased by turns based on the heart function classified by Killip grade 1-4 and the differences were significant in each group(P<0.01). The heart function classification of Killip in the acute stage following AMI positively correlated with the BNP and QRS interval(r=0.556 and r=0.385). The log BNP and QRS interval were significantly higher in patients with heart failure than those in the control group(P<0.01). LVEF of the recovery phase negatively correlated with the BNP and QRS interval(r=-0.577 and r=-0.502). The BNP>596pg/mL and QRS interval>100ms in the acute phase were both independent prognostic factors of heart failure in the immediate future following AMI(BNP OR=13.512, P=0.009; QRS interval OR=4.696, P=0.055).
    Effect of total hip arthroplasty in ankylosing spondylitis with tetanic deformity of the hip
    LIU Wen-guang,WANG Shao-jin,WANG Xing-shan,DUAN Yuan-tao,LIU Sheng-hou
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  192-195. 
    Abstract ( 1203 )   PDF (398KB) ( 685 )   Save
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    To explore the effect of total hip arthroplasty (THA) in patients with ankylosing spondylitis (AS). Methods9 patients with AS underwent total hip arthroplasty on both lateral hips at the same time with a lateral incision(bone cement prosthesis was used in 2 patients and no cement prosthesis in 7 patients), after the operations they received systemic healing exercises and were followed up for 6 to 26 (mean 18) months. The Harris grade system and Xray examination were used to evaluate the curative effects. ResultsThe postoperational movement range was significantly improved, and all of the flexion deformities were nearly rectified. According to their X-rays, there were also no dislocations, aseptic loosening or fractures of the prosthesis. The recent Harris hip scores of these patients after THA were 59 to 90 and the average scores was 76.4. ConclusionTHA is effective in treating patients with ankylosing spondylitis.
    Detection of lung surfactant proteinA and D in pleural effusions due to lung adenocarcinoma and tubercular pleurisy
    CUI Ji-yun,TIAN Guang-yan,LIN Dian-jie,LONG Cheng-feng,CHU Kui-sheng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  196-199. 
    Abstract ( 1026 )   PDF (288KB) ( 631 )   Save
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    To study the value of combined determinations of surfactant -associated protein-D(SP-D) and -A (SP-A) in the pleural fluid in distinguishing lung adenocarcinoma and tubercular pleurisy. Methods SP-A and SP-D were determined in the pleural effusions from 36 patients with lung adenocarcinoma and 28 with tubercular pleurisy by western blotting. Results SP-A concentrations in pleural effusions due to lung adenocarcinoma and tubercular pleurisy were 265.1±141.16 and 180.0±64.1 respectively, and SP-D concentrations were 299.9±140.6 and 206.8±86.8, respectively. SP-A>250 was found in 23 of 36 lung adenocarcinomas,SP-D>250 in 16 of 36 lung adenocarcinomas, while increased values of SP-A and/or SP-D were found in pleural effusions from 29 of 36 lung adenocarcinomas. SP-A and -D values did not exceed 250 in any of 28 patients with tubercular pleurisy. Conclusions SP-A is a useful immunohistochemical marker for lung adenocarcinoma, and SP-A in pleural effusion is valuable in diagnosis of primary lung adenocarcinoma and tubercular pleurisy, however, a combination of SP-D and -A assays in pleural effusions will be better for discriminating lung adenocarcinoma from tubercular pleurisy.
    Expressions of kallikrein 7 and 14 in human epithelial ovarian cancer and its clinical significance
    GU Shuang,WANG Qing,ZHANG Yi-ming,YUAN Cai-xia,ZHANG Xiang-ning
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  200-203. 
    Abstract ( 1109 )   PDF (353KB) ( 580 )   Save
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    To evaluate the expressions of kallikrein 7(KLK7) and 14(KLK14) in human epithelial ovarian cancers and to discuss its clinical significance in malignant tumors. Methods RT-PCR and Westernblot were used to determine the expressions of KLK7 and KLK14 mRNA and proteins in 50 epithelial ovarian cancers with different clinical stages and different histological grades. ResultsThe expressions of KLK7 mRNA and protein in ovarian cancers were significantly higher than those in benign tumors(P<0.01)and were markedly associated with the histological differentiation (P<0.01), also the expressions of KLK14 mRNA and protein in ovarian cancers were higher than those in benign tumors(P<0.05)and were associated with the histological differentiation(P<0.05), and the expression of KLK14 in advanced stages (Ⅲ and Ⅳ stage) was higher than that in early stages (Ⅰ and Ⅱ stage, P< 0.01). ConclusionKLK7 and KLK14 play potential roles in the pathogenesis and metastasis of ovarian cancer.
    Reinforcing the kidney and activating the blood circulation in treating luteinized un-ruptured follicle syndrome
    WANG Jiu-ling,ZHU Min-hua,LI Shu-ling,LI Hui
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  204-206. 
    Abstract ( 1296 )   PDF (246KB) ( 720 )   Save
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    To explore the effects of herbs that reinforce the kidney and activate blood circulation in treating patients with luteinized un-ruptured follicle syndrome(LUFS). Methods60 patients with the polycy polycystic ovarian syndrome after facilitating ovulation appeared LUFS were divided into the treatment group and the control group. When the follicle matured, the treatment group was given Cupailuan decoction while the control group was given human chorionic gonadotropin (HCG) for two periods of treatment. The luteinizing hormone(LH)level, the Estradiol(E2) level, ovarian artery blood flow, ovulation percentage and gestation percentage were determined. ResultThe treatment group had a better effect in improving the LH level, the E2 level, the ovulation percentage and the gestation percentage than the control group(P<0.05). The pulsating index and the resistance index of ovarian artery blood flow both decreased in the two groups (P<0.05). Conclusions Herbs that reinforce the kidney and activate blood circulation have better effects in patients with LUFS without the ovarian hyper-stimulation syndrome and it is a method worth popularizing.
    Expressions of Elastin and Lysyl oxidase in the cardinal ligament of premenopausal pelvic organ prolapse
    ZHANG Lin-lin,YU Hao,ZHANG Shi-qian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  207-209. 
    Abstract ( 1450 )   PDF (337KB) ( 620 )   Save
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    To investigate the Elastin and 1ysyl oxidase(LOX)expressions in the cardinal ligament of premenopausal women with pelvic organ prolapse(POP)so as to determine the alteration that contributes to POP. MethodsCardinal ligament samples were obtained from 42 premenopausal POP subjects and 27 non POP control women that underwent a hysterectomy. Reverse transcription polymerase chain reaction(RT-PCR)was used to verify the mRNA level of Elastin and LOX. The concentration of Elastin and LOX was determined by the Western blotting technique, electrophoretic separation and quantification. Results The POP group demonstrated a significant decrease of expressions of Elastin and LOX in the cardinal ligament than in the control group (P<0.05). There was an identical tendency in protein level of these two groups (P<0.05). Also, there was a positive correlation between the two groups(P<0.05). ConclusionThe significant decrease in Elastin production in the cardinal ligaments may lead to a loss of functional tissue elasticity, a condition that may progress to the development of POP, and collagen and elastin metabolism in POP is altered by LOX. This alteration results in less flexible connective tissue, which is probably more likely to break. LOX might be involved in the pathogenesis of POP.
    Protective effects of Cinepazide Maleate on myocardial ischemia-reperfusion injury during open heart surgery
    WANG Er-min,JIANG Jian-ge,LI Zhi-kui
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  210-213. 
    Abstract ( 1131 )   PDF (288KB) ( 569 )   Save
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    To investigate the protective effects of Cinepazide Maleate on myocardial ischemia-reperfusion injury during open heart surgery. Methods Thirty adult patients subjected to open heart surgery were randomly divided into the experimental group (group E, n=15) and the control group (group C, n=15). Each group underwent mild hypothermic extracorporeal circulation, and 160mg (4mL)Cinepazide Maleate was added into the cold bloody cardioplegia solution in group E and physiological saline (4mL) was added into group C. The creatine kinaseMB isoenzyme (CKMB) and cardiac Troponin I (cTnI) levels at 0.5, 5 and 24h after aortic crossclamp removal were determined and the corresponding changes in hemodynamics were observed. Results① The self-rebeating rate of hearts was significantly higher in group E than in group C (73% vs 33%) (P<0.05). ② Changes in hemodynamics were not significantly different between the two groups, but the dose of dopamine in 24 hours was significantly lower in group E than in group C[(382±28)mg vs (404±23)mg] (P<0.05). ③ Periods of postoperative mechanical ventilation, and hospital ICU stay were significantly shorter in group E (P<0.05), also the postoperative stay of group E was shorter than that of group C, but there was no obvious difference between the them. ④ After aortic crossclamp removal, CKMB levels at 0.5, 5, 24h and cTnI levels at 0.5h were significantly lower in group E than in group C (P<0.05), even more, ConclusionCinepazide Maleate could enhance myocardial protective effects on ischemia-reperfusion injury during open heart surgery, and it significantly decreases the CKMB and cTnI levels and improves cardiac functional restoration.
    Nitroglycerin combined with esmolol in controlled hypotention in spinal tumor surgery
    WANG Shu-qin,MENG Qing-li,LIU De-jie,YU Jin-gui
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  214-216. 
    Abstract ( 1282 )   PDF (285KB) ( 376 )   Save
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    To compare the anesthesia effects of nitroglycerin and esmolol in inducing controlled hypotention during spinal surgery. MethodsThirty patients undergoing elective spinal tumor surgery were randomly divided into three groups(n=10): the control group(A), the nitroglycerin group(B) and the nitroglycerin+esmolol group(C). Group A only received normal saline without controlled hypotention, group B initially received nitroglycerin at 2.5μg/(kg·min) and group C initially received nitroglycerin[2.5μg/(kg·min) ]and esmolol[12.5μg/(kg·min)]. The nitroglycerin dosage was adjusted to maintain the MAP level at 6070 mmHg in groups B and C. Hemodynamic parameters were recorded before controlled hypotention, just at the controlled hypotention, 30?min after controlled hypotention, at the end of controlled hypotention and at the end of the operations. Also the operation time, intraoperative blood loss, blood transfusion amounts and postoperative drainage from the wound were recorded. ResultsThe intraoperative blood loss and blood transfusion cases in groups B and C were much less than those in group A(P<0.05). The MAP level of groups B and C significantly decreased during controlled hypotention(P<0.01). The HR level of group B significantly increased during controlled hypotention(P<0.05)and of group C was less than that in group B (P<0.05). The duration of the operation was significantly shorter in group C than in group A.
    Effect of MARS on severe hepatitis and its related factors
    ZHAO Xiu-hua,ZHANG Jing-yao,SONY Xiu-guang,WANG Xiao-hua,SHAO Fei,CHEN Shi-jun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(2):  217-220. 
    Abstract ( 1099 )   PDF (259KB) ( 575 )   Save
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    To analyze the treatment effects of the molecular adsorbent recycling system (MARS) for severe hepatitis and the related factors. Method123 patients with severe hepatitis were subjected to MARS as well as basic treatments. The effects of MARS and the related factors, such as the clinical types and stages of severe hepatitis, age of patients, and treatment times were analyzed. Result 48 patients were cured or improved (39%). The cure rates of early, mediate and advanced severe hepatitis were 80.8%, 46.5% and 13% respectively and of acute, subacute and chronic severe hepatitis were 14.3%, 55.6% and 42.9% respectively. The treatment effects of MARS had no positive relationship with the treatment times, but had some relationship with age. Conclusion MARS is effective in treating severe hepatitis, which is related to the age of patients and the clinical type and stage of severe hepatitis but not to the treatment times.