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Table of Content

      
    16 November 2008
    Volume 46 Issue 11
    Articles
    Association of the -964A>G polymorphism in the IL-27p28 gene promoter region and asthma in a Chinese Han population
    QIU Rong-fang,LI Ji-sheng,GAO Gui-min,GUO Chen-hong,LI Huai-chen,WEI Chun-hua,LIU Qi-ji
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1021-1023. 
    Abstract ( 1035 )   PDF (352KB) ( 302 )   Save
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    To genotype a single nucleotide polymorphism (SNP), -964A>G, in the IL27p28 gene promoter region and evaluate the association between SNP and asthma susceptibility in a Chinese Han population from Shandong Province. MethodsSNP -964A>G was genotyped in 360 asthma patients and 220 healthy controls by using the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) method. The Chi-square test was used to evaluate the Hardy-Weinberg equilibrium. Allele frequencies and significance values were analyzed by using SPSS13.0 software. ResultsSNP 964A>G was genetically polymorphic in the Chinese Han population. The distribution of this SNP was at Hardy-Weinberg equilibrium in both asthma patients and healthy controls. The frequencies of genotypes AA, GA, and GG at -964A>G site were 0.4028, 0.4205, and 0.1722 in the asthma patients, while 0.4318, 0.4455, and 0.1227 in the healthy controls. No statistically significant difference was observed in the distribution of genotype and allele frequency between asthma patients and healthy controls (all P>0.05). ConclusionsSNP -964A>G in the IL-27p28 gene promoter region is not associated with asthma in the Chinese Han population
    Effects of mechanical ventilation on CC10 protein level in lung tissues in rats
    SONG Xiu-mei,WANG Yue-lan,LI Cheng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1024-1027. 
    Abstract ( 1047 )   PDF (507KB) ( 342 )   Save
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    To investigate the effects of mechanical ventilation on CC10 protein levels in lung tissues of rats and the effects of CC10 on lung inflammation reactions. MethodsThirty normal Wistar rats weighing 200-250g were randomly divided into 3 groups (10 in each):the control group, the low tidal volume group (tidal volume VT=8ml/kg, ventilation time t=4h), and the high tidal volume group (VT=40ml/kg, t=4h). After ventilation, the rats were sacrificed and the lungs were removed. Levels of CC10, TNF-α and MIP-2 were determined by ELISA. The percentage of Clara cells in the bronchiolar epithelium and PMN was determined in the broncho-alveolar lavage fluid (BALF). ResultsHigh tidal volume ventilation induced serious histopathological damage in lung tissues of rats. Both TNF-α and MIP-2 in lung tissues and PMN in BALF significantly increased in the high tidal volume group as compared with the control group and the low tidal volume group (P<0.05). The levels of CC10 in lung tissues and the percentage of Clara cells was significantly lower in the high tidal volume group than those in the control and low tidal volume groups(P<0.05), but they were not statistically different between the control and the low tidal volume groups (P>0.5). ConclusionHigh tidal volume ventilation decreases the expression of CC10 in lung tissues and increases the expressions of TNF-α,nd MIP-2, and induces heavy lung injury.
    Expression profiling construction of human pancreatic juice protein
    TIAN Mei,CUI Ya-zhou,ZONG Mei-juan,CHEN Yu,HAN Jin-xiang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1028-1033. 
    Abstract ( 992 )   PDF (701KB) ( 409 )   Save
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    ObjectiveTo construct an expression profiling of human pancreatic juice protein. MethodsPancreatic juice samples from four patients who were not suffering from cancer of the pancreas or ducts were treated by using an acetone precipitation method. Pancreatic juice proteins were separated and identified by using two-dimensional electrophoresis (2-DE) and matrixassisted laser desorption/ionization-time of flight tandem mass spectrometry (MALDI-TOF-MS/MS). ResultsThe expression profiling with a high resolution of human pancreatic juice proteins was constructed. Ninety-one unique proteins were identified and functional annotation analysis was performed. Conclusions Protein expression profiling can provide a theoretical basis for further understanding the physiological and pathological mechanisms involved in pancreatic diseases, and screening and for finding potential biomarkers for pancreatic cancer.
    Effect of valproic acid on angiogenesis of human ovarian cancer in nude mice
    YANG Gao-yuan,RONG Feng-nian,ZHOU Ting
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1034-1036. 
    Abstract ( 1062 )   PDF (517KB) ( 296 )   Save
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    To investigate the inhibitory effect of valproic acid(VPA)on angiogenesis of human ovarian cancer in nude mice. MethodsHuman ovarian cancer models were established by subcutaneous transplantation in nude mice, and then they were divided into 4 groups: the control group, the VPA group, the cisplatin (DDP) group and the VPA+DDP group. The anti-tumor efficacy was evaluated by determination of tumor volume, tumor growth and relative tumor curative rate. Also expression levels of CD105 and sFlk-1 were determined by immunocytochemistry (IC). Results① Tumor volumes in the experimental groups were smaller than those in the control group; ② Expression levels of CD105 in the experimental groups were significantly higher than those in the control group(P<0.05); and ③ Expression levels of sFlk-1 in the experimental groups were significantly higher than those in the control group(P<0.05). ConclusionsVPA can inhibit the growth of ovarian cancer by decreasing the expression of CD105 and increasing the expression of sFlk-1 levels.
    Therapeutic role of CD28 monoclonal antibody in mice with viral myocarditis
    ZHANG Yi,HAN Bo,ZHAO Li-jian,LIU Zhen-hu,XI Jun-fang,LU Kang,HAN Xiu-zhen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1037-1040. 
    Abstract ( 1047 )   PDF (461KB) ( 352 )   Save
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    To investigate the effect of the CD28 monoclonal antibody (CD28 mAb) on mortality, viral titers of myocardium and pathologic changes of myocardium in CVB3 myocarditis in mice. MethodsA total of 106 BALB/c male mice were used in the experiments, which were randomly divided into the CD28 mAb group (16 mice), the CVB3 group (40 mice), the IgG group (40 mice) and the control group (10 mice). Mice in the former three groups were intraperitoneally inoculated with 0.15?mL CVB3 (TCID50 10-6/mL) and in the control group with 0.15?mL eagle, and then mice in the IgG and the CD28 mAb groups were inoculated with IgG(0.1mg/kg) and CD28 mAb(0.1mg/kg) at 6 hours and 72 hours post-inoculation(p.i.). The surplus mice in each group were sacrificed on day 7. The heart was taken and pathologic changes of cardiac muscle cells were observed under light microscopy, and expression of CVB3 mRNA of myocardium was determined by the realtime quantitative polymerase chain reaction(RQ-PCR) method. ResultsThe mice mortality rate in the CD28 mAb group was lower than that in the CVB3 group (80% vs 50%, P<0.05); the pathological score(8.88±2.95 vs 5.25±1.91, P<0.05) and the CVB3mRNA expression level(3.48±2.89 vs 0.51±1.80, P<0.05). ConclusionCD28 mAb could decrease the expression of CVB3 mRNA, relieve myocardium inflammation and reduce the mortality rate.
    Expressions of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 after diffuse brain injury and their significance in rats
    SONG Lin-jie,ZHANG Xiu-ping,WANG Chong,DONG Ya-nan,JIANG Yong,ZHAO Hong-yang,ZHU Xian-li
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1041-1044. 
    Abstract ( 979 )   PDF (492KB) ( 245 )   Save
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    To explore whether expressions of matrix metalloproteinase-9 and tissuce inhibitor of metalloproteinase-1 in early diffuse brain injured tissues have a correlation with second brain injury in rats. Methods Real-Time quantitative PCR was used to determine expressions of MMP-9mRNA and TIMP-1mRNA, the dry-wet method to determine the containing water measure in the brain, and histological techniques and electron microscopy to determine the ultrastructural blood brain barrier at different time-points after diffuse brain injury in rats. Results Compared with the sham-operated group, expression of MMP-9mRNA started to increase at the first hour, peaked at the 12th hour(P<0.01) and persisted up to one week. Expression of TIMP-1mRNA also peaked at the 24th hour and persisted up to one week (P<0.01). Ultrastructures of the neurons were injured, edema and necrosis were diffuse and distinct at the 72 th hour under light microscopy and electron microscopy. ConclusionsExpressions of MMP-9mRNA and TIMP-1mRNA are increased after diffuse brain injury in rats, indicating that MMP-9 and TIMP-1 are closely related to brain edema and neuronal damage in early second brain injury in rats.

    Relationship between myocardial acoustic densitometry and matrix metalloproteinase in essential hypertensive patients

    ZHANG Yan-min,DU Nai-li,DU Rui-zhi,HAO En-kui
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1045-1048. 
    Abstract ( 958 )   PDF (276KB) ( 420 )   Save
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    To evaluate the clinical value of quantitative myocardial acoustic densitometry in myocardial fibrosis in relative to an alteration in serum metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) concentrations in essential hypertensive patients. Methods72 essential hypertensive patients were divided into two groups based on the left ventricular mass index: 40 patients with left ventricular hypertrophy(the LVH group) and 32 patients without left ventricular hypertrophy(the NLVH group), and 30 normal persons served as healthy controls. Calibrated acoustic intensity in the septal(CAl1) and posterior wall(CAI2) and cyclic variation of integrated backscatter in the septal(CVIB1)and posterior wall(CVIB2) were determined by the AD technique. Concentrations of MMP-9 and TIMP-1 were determined by enzyme-linked immunosorbent assays (ELISA). ResultsCAI1 and CAI2 in the LVH group(46.12±4.52 and 45.84±5.71, respectively) were significantly higher than those in the NLVH group (41.61±5.98 and 41.42±5.62, respectively) and the control group (38.30±3.87 and 37.18±3.88, respectively)(P<0.01). However, CVIB1 and CVIB2 in the LVH group(7.19±1.27 and 8.99±1.35, respectively) were significantly lower than those in the NLVH group (8.59±1.89 and 10.23±1.80, respectively) and the control group (9.29±2.11 and 10.92±2.10, respectively)(P<0.01). MMP-9 and TIMP-1 concentrations in the LVH group (32.75±4.95 and 83.21±32.65, respectively) were significantly higher than those in the NLVH group(28.20±5.70 and 60.09±28.51,respectively) and the control group (25.26±5.97 and 38.18±10.61, respectively)(P<0.01), while the MMP-9/TIMP-1 ratio in the LVH group (0.46±0.24) was significantly lower than that in the NLVH group (0.57±0.21) and the control group (0.69±0.19) (P<0.05). MMP-9 level in the LVH group was positively related to CAI1 and CAI2(0.71 and 0.78, respectively)(P<0.01), which was inversely related to CVIB1 and CVIB2(-0.80 and -0.70, respectively)(P<0.01). Also the TIMP-1 level in the LVH group was positively related to CAI1 and CAI2(0.79 and 0.80, respectively)(P<0.01). ConclusionMyocardial acoustic densitometry parameters are significantly related to MMP-9 and TIMP-1, which may be potentially used for evaluation of severity of myocardial fibrosis.

    Stepwise ablation for the treatment of atrial fibrillation based on circumferential pulmonary vein ablation

    LI Xiang-ting,JIAO Feng-zhen,DU Zhong-chun,LIU Tong-bao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1049-1052. 
    Abstract ( 1234 )   PDF (268KB) ( 396 )   Save
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    To investigate the feasibility and efficacy of a stepwise ablation procedure in treatment of atrial fibrillation(AF) on the basis of circumferential pulmonary vein(PV) ablation guided by a three-dimensional mapping system(Carto). MethodsTwelve patients with drug-refractory paroxysmal (n=10) and persistent (n=2) atrial fibrillation were subjected to three-ablation stages during one session: circumferential ablation around the pulmonary vein antrum, segmental pulmonary vein ablation, and ablation of complex fractionated atrial electrograms (CFAEs). Endpoint of the procedure was a non-inducibility of AF at any stage or completion of all three-ablation stages. ResultsTen patients with a paroxysmal AF had a non-inducible AF at the end of the procedure: seven patients after the first stage, two after the second and one after the third. Two patients with a persistent AF who remained AF after all stages required an external cardioversion. Three patients had a recurrent AF within two days, but were free of recurrent AF after further follow-up. One patient with a paroxysmal AF and typical atrial flluter(AFL) had a recurrent AFL and was free of recurrent AF. During 10±4 months of follow-up, all patients were free of recurrent AF or had left atrial arrhythmias without an anti-arrhythmic drug therapy, and no complications occurred. Average radiofrequency energy delivery, fluoroscopy time and procedure time were (38±11) min, (37±11) min and (3.0±0.5) h, respectively. ConclusionA stepwise ablation procedure on the basis of circumferential pulmonary vein ablation with the AF noninducibilty as a endpoint is effective and safe. Circumferential pulmonary vein ablation alone is enough for paroxysmal AF treatment, while it combined with segmental pulmonary vein ablation and CFAEs ablation may result in high successful rates.
    Mentality and behavior of children with congenital ventricular septal defect
    ZHANG Kun,WANG Li-hua,LI Fu-hai,XIA Wei,SUN Ruo-peng,WANG Yi-biao,HAO Fang-zhi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1053-1056. 
    Abstract ( 976 )   PDF (284KB) ( 311 )   Save
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    To investigate the mentality and behavior of children with congenital ventricular septal defect(VSD) and to explore the influence of interventional versus surgical procedure of VSD on mentality and behavior. Methods From January 2006 to May 2007, 132 children suffering from VSD, without associated structural cardiac anormalies, were divided into two groups: the interventional group(n=68) and the surgical group(n=64). The Achenbach Child Behavior Checklist(CBCL) edited by Xu Tao-yuan in 1992 was performed on these children before and 6 months after treatment. Sixty sex, age, education and achievement-matched normal children were enrolled as controls. ResultsMentality and behavior of children with VSD were abnormal. The total behavioral problem scores of the VSD children were significantly higher than those of the controls (P<001). Behavior abnormities of boys were depression, social flinch, physical complaints, assaults and violation of rules. Whereas girls presented depression, social flinch, physical complaints and violation of rules, which was significantly different from normal children(P<0.01 or P<0.05). The abnormal rates of mentality and behavior positively correlated with course of disease. The total cursory mark of the postoperative check result of the interventional and surgical groups, both in girls and boys, was evidently lower than those of the pre-operative children (P<0.05). The total and assault cursory mark of the post-operative check result of the interventional boys were lower than those of the surgical ones(P<005). The total and depression, social flinch, violation of rules cursory mark of the post-operative check result of the interventional girls were lower than those of the surgical ones (P<0.05). ConclusionVSD affects mentality and behavior of children, which should be paid more attention to, also early treatment and effective and safe. Circumferential pulmonary vein ablation alone is enough for paroxysmal AF treatment, while it combined with segmental pulmonary vein ablation and CFAEs ablation may result in high success rates.
    Relationship between activin A and hypoxic ischemic encephalopathy in neonate with asphyxiation
    LUO Huan-hua,ZHENG Yuan,AN Li
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1057-1059. 
    Abstract ( 972 )   PDF (255KB) ( 382 )   Save
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    To explore the changes of endogenous activin A (ACT A) in neonates with asphyxia and the relation between ACT A and hypoxicischemic encephalopathy (HIE). MethodsSerum ACT A, FGF and TNF-α were determined at postnatal 1 day (P1, cord blood) and postnatal 7 day (P7) in 58 term infants with asphyxia and 30 normal controls by enzyme-linked immunosorbent assay (ELISA). Clinical grading was performed based upon related clinical data. ResultsConcentrations of serum ACT A, FGF and TNF-α were much higher in neonates with asphyxia than in controls at P1 (P<0.01). There was a negative correlation between the level of serum ACT A and Apgar scores at 1 minute after birth(r=-0.948, P<0.01). Also concentrations of serum ACT A and FGF were higher in neonates with asphyxia than in controls at P7 (P<0.01). However, concentration of serum TNF-α was not significantly different between neonates with asphyxia and normal controls(P>0.05). Based on clinical grading of HIE, related clinical data was scored in neonates with asphyxia. Correlation analysis between the level of serum ACT A and clinical scores showed that there was a positive correlation between the level of serum ACT A and severity of HIE(r=0.962,P<0.01). ConclusionEndogenous ACT A plays a role in the pathogenesis of HIE. Dynamic detection of serum ACT A may be helpful to evaluate the severity and prognosis of HIE.
    Plasma t-PA and PAI-1 levels in patients with type 2 diabetes and the effect of pancreatic kallikrein
    SUN Chao,YIN Xiao, PU Ying, SUN Jin-feng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1060-1062. 
    Abstract ( 1131 )   PDF (239KB) ( 248 )   Save
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    To investigate the relationship of chronic diabetic vascular complication and plasma t-PA and PAI-1 levels in type 2 diabetes and the effect of pancreatic kallikrein(TPK)on them. Methods88 patients with type 2 diabetes and 30 healthy controls were recruited into the study. Plasma t-PA and PAI-1 levels were determined by ELISA. Measurements were also carried out before and after the interfered treatment of TPK. ResultsCompared with the healthy controls, the diabetes patients showed a decreased plasma t-PA level and an increased PAI-1 level (P<0.05 or<0.01). The plasma t-PA level in diabetes patients with vascular complications was lower and the PAI-1 level was higher than those without vascular complications (both P<0.01). The plasma t-PA level increased and the PAI-1 level decreased in diabetes patients after the treatment of TPK. tPA had a negative relationship with plasma TG and LDL-c levels, and PAI-1 had positive relationships with BMI, plasma TG and LDL-c levels, FBG, SBP and HbA1c. ConclusionPlasminogen dysfunction is an essential factor in the occurrence and development of diabetic vascular complications. The interfered treatment of TPK may prevent the development of type 2 diabetes and its vascular complications.

    Diagnostic value of transbronchial needle aspiration in paratracheal mediastinal lesions

    LI Wei,LI Dao-wei,YE Qian,GAO Shun-cui,JIANG Shu-juan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1063-1065. 
    Abstract ( 1074 )   PDF (445KB) ( 352 )   Save
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    To investigate the significance of transbronchial needle aspiration (TBNA) in diagnosing paratracheal mediastinal lesions. MethodsTBNA procedures were performed by using a flexible bronchoscope in 168 consecutive patients who had a mediastinal mass diagnosed by CT. ResultsThe positive diagnostic rate was 92.9% for TBNA with a tumor diagnosis rate of 81.0%. A few patients had a little bleeding at the puncture site without mediastinal emphysema or infection. ConclusionsTBNA is an effective and safe way for obtaining cytologic specimens from intrathoracic lymph nodes and can rapidly provide diagnosis for paratracheal mediastinal lesions.
    Clinical features and surgical treatments for primary middle lobe carcinoma of the lung
    LI Jun,LI Yu,WANG Zhou,WANG Huan-yuan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1066-1068. 
    Abstract ( 1135 )   PDF (255KB) ( 385 )   Save
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    To investigate the features of clinical performances and surgical treatments for patients with primary middle lobe lung carcinoma so as to enhance the diagnostic and survival rate. MethodsThe clinical data and therapeutic results of 106 patients with middle lobe lung cancer who had undergone surgical treatment were retrospectively studied. ResultsThere were 62 patients with cough, 48 with bloody sputum, 29 with fever, 16 with chest pains and 8 with joint pains. Thirty patients were subjected to antiinflammatory treatments for more than 30 days and 9 anti-tuberculosis treatments. Sixtyfour cases were diagnosed within 1 month, 25 within 1 to 6 months, 13 within 7 to 12 months and 4 within 13 to 18 months. Three patients underwent exploratory thoracotomy and 103 patients underwent resection including middle lobectomy in 32 patients, middle-lower lobectomy in 51, middleupper lobectomy in 13, and right pneumonectomy in 7. Altogether 333 N1 and 378 N2 lymph nodes were cleared up. Five patients underwent lateral wall resection of the right lung lower artery and 2 patients underwent artery sleeve resections. One patient died of respiratory failure after the operation. Based on pathologic results, positive lymph nodes of PN1 were identified in 8 cases, PN2 in 6 cases, and both PN1 and PN2 in 21 cases. There were 14 cases in pathological stage I, 34 cases in pathological stage Ⅱ, 51 cases in pathological stage ⅢA, 3 cases in pathological stage ⅢB and 1 case in pathological stage Ⅳ. Followup: The 5year survival rate of the 97 patients was 29.9%. ConclusionsMiddle lobe pulmonary carcinoma sometimes is misdiagnosed as inflammation or tuberculosis, so a reasonable checkup should be performed in time to make a final diagnosis. Because of the anatomic features of middle lobar bronchus, lobectomy in combination with clearance of the mediastinal lymph node should be performed.
    Effects of probiotics supplementation on intestinal symptoms and immunity of preterm infants
    ZHANG Feng-juan,SUN Zheng-yun,CHENG Bei-lei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1069-1071. 
    Abstract ( 1054 )   PDF (297KB) ( 378 )   Save
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    To investigate the effects of probiotics supplementation on intestinal symptoms and immunity of preterm infants. MethodsFifty preterm infants born and hospitalized in the neonatal intensive care unit of our Hospital from June 2007 to December 2007 were randomly divided into the control group and the probiotic group. The probiotic group received probiotics on day 0 to 14 after birth. Intestinal symptoms were observed during hospitalization and blood samples were collected on day 14 to 16 after birth. ResultsThe number of warm brine enema of the probiotic group was lower than that of the control group [(0.36±0.70) vs (0.92±1.12), P<0.05]. The proportion of CD4+ T lymphocytes and the ratio of CD4+/CD8+ were increased in the probiotic group, but the proportions of CD3+ and CD8+ T lymphocytes were not statistically changed in the probiotic group. The levels of immunoglobulin M and interlukine4 were increased in the probiotic group, but the level of IFNγand the ratio of IFNγ/ IL4 were not statistically changed in the probiotic group. ConclusionProbiotics could improve intestinal symptoms of preterm infants and have an obvious effect on their immunity.
    Bone grafting in total hip replacement in patients with acetabulum deficiency
    DUAN Yuan-tao,WANG Shao-jin,LIU Wen-guang,WANG Cheng,LIU Sheng-hou
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1072-1074. 
    Abstract ( 965 )   PDF (457KB) ( 313 )   Save
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    To explore the clinical effect of bone grafting in total hip replacement(THR) for hips with acetabulum deficiency. Methods58 patients (75 hips) with acetabulum deficiency had THR performed. In the operations, an ural bone graft, a morselized bone graft or their combination were used based on the definition. After the operations, 46 patients (59 hips) were followed up and evaluated by X-rays and the Harris score. ResultDuring a followup of 45.6 months, 54 cases (91.53%) were cured, and 5 cases (8.47%) had bone graft absorption. The hip functions were excellent in 23 cases (28 hips), good in 20 cases (26 hips) and fair in 3 cases(5 hips). Conclusion Acetabulum reconstruction with auto graft in THA will bring good stability in patients with acetabulum deficiency, and also it is of significance in maintaining longterm function in the replaced hip.
    High resolution CT measurements of the normal inner ear
    YANY Ying,XIA Ming,BIAN Zhi-qiang,XU An-ting
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1075-1079. 
    Abstract ( 1127 )   PDF (587KB) ( 481 )   Save
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    To explore the clinical value of normal inner ear structures. Methods75 recipients without inner ear abnormalities were divided into three groups based on age. All underwent HRCT, the images were transferred to an online workstation for analysis. All cochlear, vestibular, internal auditory cannel (IAC), and the LSCC bony island width were determined. ResultsStructures of the inner ear and internal auditory cannel could be clearly visualized by HRCT. Excluding differences of IAC length in age, no age, side or race-related differences in other inner ear structures were found. The cochlea width and height were (6.27±0.96)mm and (3.47±0.41)mm, the basal turn of cochlea length and lumen diameter were (6.82±0.69)mm and (1.66±0.26)mm, the vestibule width was (2.81±0.42)mm, the vestibule height was (5.76±0.44)mm, the aperture of the inner ear auditory meatus was (6.36±1.66)mm, the width of the internal auditory meatus was (4.99±0.98)mm and the LSCC bony island width was (3.16±0.52)mm. ConclusionThe HRCT measurement criterion of normal inner ear structures was established.
    Relationship between loss of residual renal function and malnutrition in peritoneal dialysis patients
    FAN Xiao-hong,CHENG Li-tao,WANG Tao,HU Zhao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1080-1083. 
    Abstract ( 1154 )   PDF (503KB) ( 471 )   Save
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    Abstract: ObjectiveTo evaluate the effect of declining residual renal function (RRF) on peritoneal dialysis patients′ nutritional status. Method32 patients whose glomerular filtration rate (GFR) had decreased by more than 50% in one year were selected for the present analysis. Patients′ residual renal function was assessed by the average renal urea and creatinine clearances. Serum albumin(ALB), dietary protein intake(DPI) and subjective global assessment(SGA) were closely followed up. ResultsRRF was significantly decreased (P<0.05) with a significant decrease of urine volume(P<0.01), while serum ALB was significantly increased (P<0.01), and also DPI was significantly increased. The prevalence of malnutrition(by SGA) was decreased from 62.5% at the start of dialysis to 18.8% at the end of this study(P<0.05). ConclusionThe present study suggests that rapid decline of residual renal function in PD patients does not necessarily lead to a decreased dietary protein intake and deteriorated nutritional status.
    Imaging parameters of multislice spiral CT venography in cerebral veins
    JIANG Bao-dong,MA Xiang-xing,WANG Qing,WANG Qian,FENG Xiao-yuan,LI Ke,YU Fu-hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1084-1086. 
    Abstract ( 1468 )   PDF (459KB) ( 653 )   Save
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    To investigate the optimal delay time and slice thickness of CT venography(CTV) of the head in order to get better images and to improve the diagnosis for cerebral venous system diseases. Methods Plane dynamic enhancement scanning was performed and a timedensity curve of the internal cerebral vein and the superior sagittal sinus was produced at the workstation in order to get the optimal delay time of the cerebral CTV. The data obtained by a scanning thickness of 1.250mm were retrospectively reconstructed with a slice thickness of 0.625mm. ResultsOptimal delay time of CTV in the head was 36s. Quality of images with a slice thickness of 0.625mm was superior to that with a slice thickness of 1.250mm. ConclusionSatisfactory CTV images of the head can be produced with an optimal delay time of 36 s and a slice thickness of 0.625mm.
    Relationship between the oestrogen receptor α gene polymorphisms and the Th1/Th2 cytokine drift in systemic lupus erythematosus
    WANG Zi-e,CHE Zhi-xiang,WU Hao,FENG Rong-jie,LIU Yi-qing,SHAO Song,CUI Xiang-fa,CHEN Guo-jian,LU Zhi-ming
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1087-1091. 
    Abstract ( 1105 )   PDF (462KB) ( 246 )   Save
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    To analyze the association of the estrogen receptor α (ERα) gene polymorphisms with the Th1/Th2 cytokine drift in systemic lupus erythematosus (SLE). MethodsGenomic DNA was extracted and polymorphisms of Xba Ⅰ and Pvu Ⅱ in intron 1 of the ERα gene were determined by the polymerase chain reactionrestriction fragment length polymorphisms (PCR-RFLP) method. The mRNA levels of IL-10, IL-4, IFN-γ and IL-2 were determined by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). ResultsThe IL-10 and IL-4 mRNA expressions of SLE patients with the PpXx genotype were higher than those of controls (P<0.01, P<0.05), however the IFN-γ and IL-2 mRNA expressions of SLE patients with the Ppxx genotype were lower than those of controls (P<0.05). The mRNA expression of four cytokines had no significant differences in various genotypes among the controls. ConclusionThe various ERα genotypes are relevant to cytokine expression in different individuals. The ERα gene polymorphisms may influence cytokine expression to some extent based on the gene background.
    Influential factors of the quality of life of adult patients with epilepsy
    XU Hong,LONG Fa-qing,ZHANG Ming-zhi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1092-1094. 
    Abstract ( 1090 )   PDF (224KB) ( 297 )   Save
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    To explore the quality of life of patients with epilepsy and to identify factors affecting the quality of life. MethodsThe Quality of Life in the Epilepsy 31 Inventory, the MOS item short from health survey 36, Self-rating Depression Scale, Self-rating Anxiety Scale were used to determine the quality of life of patients with epilepsy. ResultsScores of the quality of life were significantly lower in epilepsy patients than in normal people. Depression, anxiety, duration of epilepsy, seizure frequency, education level, seizure sum, age of epilepsy onset, and family education were the most significant factors affecting the quality of life(P<0.05). Gender and marital status were not factors influencing the quality of life(P>0.05). ConclusionThe quality of life of patients with epilepsy is significantly poorer than that of normal people. Depression and anxiety are the most significant factors affecting the quality of life and the most significant predictors for the quality of life.
    Expression of soluble endoglin and sFlt-1 in peripheral blood of pre-eclampsia
    XING Jing,CHEN Li-jun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1095-1097. 
    Abstract ( 1031 )   PDF (233KB) ( 311 )   Save
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    To investigate the expression and significance of serum soluble endoglin(sEng) and soluble fmslike tyrosine kinase-1 (sFlt-1) in pre-eclampsia. MethodsSerum levels of sEng 1 and sFlt-1 were determined by enzyme linked immunosorbent assay. ResultsThe serum level of sEng of the preeclampsia group (5.27±1.05)ng/mL was significantly higher than that of the controls [(1.26±0.40)ng/mL,P<0.01], especially of early-onset severe pre-eclampsia. The serum level of sFlt1 of the preeclampsia group (391.14±70.25)pg/mL was significantly higher than that of the third trimester group (27.57±4.36)pg/mL, but sFlt-1 did not correlate with the most severe forms of the disease. ConclusionSerum levels of sEng and sFlt-1 are significantly increased in preeclampsia, and the serum level of sEng is even higher in severe pre-eclampsia patients.
    Changes of the maxillary dentoalveolar and dental arch produced by In-Ovation selfligating system during the leveling andaligning phases of treatment
    ZHANG Wei-wei,MAO Yu-long,WEI Lin,GUO Jing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1098-1101. 
    Abstract ( 1037 )   PDF (445KB) ( 385 )   Save
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    To evaluate changes in the maxillary dentoalveolar and dental arch produced by the In-Ovation self-ligating system during the leveling and aligning phases of treatment. MethodsThe In-Ovation self-ligating system was used in 20 patients (13 females and 7 males, 17 to 35 years old) with mild crowding in the maxillary arch to level and align the maxillary dentition. Dental casts of the maxillary arch were taken at T1(immediately before treatment)and T2(at the end of leveling and aligning phase with 0.016in Ni-Ti archwires). All dental casts were scanned by a special laser scanner and treated with Image ware software to install digital models, and then many landmarks of dentoalveolar and dental arch were selected in the digital models. All data were analyzed by SPSS12.0 software, and changes before and after treatment in dentoalveolar and the dental arch widths, and the depth and perimeter of the maxillary arch were determined. ResultsCompared with T1, statistically significant increases were recorded in all dentoalveolar and dental arch widths, except for the intermolar width measured fossa, and also arch perimeter at T2 (P<0.05). No significant changes were found in the arch depth. ConclusionDuring the initial phase of leveling and aligning, the In-Ovation selfligating system produces significant increases in the transverse dentoalveolar, the dental arch width and the perimeter of the maxillary arch.
    Epidemiological survey of sleep-disordered school children in the Jinan district
    FU Li,WANG Yan,LI Yan-zhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1102-1104. 
    Abstract ( 1307 )   PDF (250KB) ( 317 )   Save
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    AbstractObjectiveTo explore the prevalence of sleep disorder in school children in the Jinan district. MethodsDuring October 2006 to September 2007, 1995 children aged 7 to 12 were selected by random and investigated by giving questionnaires to their parents. Result1596 efficient questionnaires were used for analysis, 762 (47.7%) of them never snore, 488(30.6%) occasionally snore (1 or 2 nights per month), 282 (17.7%) snore 1 or 2 nights per week, 64 (4.0%) snore every night, and 31(1.9%) was found with apnea. The relevant dangerous factors included bad nose respiration, male gender and parent who snore. The relevant dangerous factors of apnea include snore, bad nose respiration and tonsillitis. ConclusionSleep disorders of children in the Jinan distract should draw the attention of parents and doctors.
    Study on methodology and application of spatial heterogeneity of disease boundaries
    TANG Fang,XUE Hao,WANG Zhi-qiang,KANG Dian-min,WANG Yi,XUE Fu-zhong,WANG Jie-zhen,
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1105-1109. 
    Abstract ( 1040 )   PDF (905KB) ( 307 )   Save
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    To explore “Monmonier′s algorithm” and its application on spatial structures of natural focal disease. MethodsThe improved Monmonier′s algorithm model based on Monmonier′s maximum-distance algorithm and distance matrix between the corresponding data, which were connected using a Delaunay triangulation, was built. Structures and boundaries of Hemorrhagic fever with renal syndrome (HFRS) were identified. Results The improved Monmonier′s algorithm model well showed the edges associated with the highest rate of changes in the given distance measure, namely the areas where differences between locations of disease are largest. The bootstrap test was used to assess the robustness of computed boundaries and infer the spatial connection and arrangement of boundaries. Conclusion The improved Monmonier′s algorithm model is useful in detecting spatial heterogeneity of disease boundary.
    HPLC-MS determination and pharmacokinetic study of glycyrrhetic acid in human plasma
    ZHAO Wen-jing,WANG Ben-jie,WEI Chun-min,YUAN Gui-yan,BU Fan-long,GUO Rui-chen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(11):  1110-1112. 
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    To develop a LC-MS method for the determination of glycyrrhetic acid in human plasma and for evaluation of its pharmacokinetic characteristics.Methods Glycyrrhetic acid in plasma was extracted with ethyl acetate, separated on a C18 column with a mobile phase of methanol(5mmol/L ammonium acetate)water(85∶15,V/V) and analyzed by the LCMS method with ursolic acid(UA) as the internal standard.The target ions were m/z 469.5 for glycyrrhetic acid and m/z 455.6 for internal standard. The fragmentor voltages were 200V for glycyrrhetic acid and 100V for the internal standard. ResultsThe calibration curve was linear over the range of 0.5 to 200ng/mL (r=0.9974). The quantitative limit for glycyrrhetic acid in plasma was 0.5ng/mL, the recovery rate was 76.0% to 80.0%, and the interday and intraday derivations (RSD) were less than 12%. The Cmax and Tmax of glycyrrhetic acid were (95.57±43.06)ng/mL and (10.95±1.32)h after oral administration. Conclusion The assay proved to be sensitive,accurate and convenient and can be applied in the determination of glycyrrhetic acid in human plasma and its pharmacokinetic study.